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Protein

Guanine nucleotide-binding protein-like 3-like protein

Gene

Gnl3l

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Stabilizes TERF1 telomeric association by preventing TERF1 recruitment by PML. Stabilizes TERF1 protein by preventing its ubiquitination and hence proteasomal degradation. Does so by interfering with TERF1-binding to FBXO4 E3 ubiquitin-protein ligase. Required for cell proliferation. By stabilizing TRF1 protein during mitosis, promotes metaphase-to-anaphase transition. Stabilizes MDM2 protein by preventing its ubiquitination, and hence proteasomal degradation. By acting on MDM2, may affect TP53 activity. Required for normal processing of ribosomal pre-rRNA. Binds GTP (By similarity).By similarity2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi166 – 1694GTPSequence analysis
Nucleotide bindingi253 – 2608GTPSequence analysis
Nucleotide bindingi297 – 3004GTPSequence analysis

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Ribosome biogenesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine nucleotide-binding protein-like 3-like protein
Gene namesi
Name:Gnl3l
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:2448557. Gnl3l.

Subcellular locationi

  • Nucleusnucleolus 1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi166 – 1661N → I: No effect on TERF1-binding. Loss of ability to stabilize TERF1 protein. 1 Publication
Mutagenesisi253 – 2531G → V: Mislocalized to the nucleoplasm. No effect on the reduction of MDM2 ubiquitination. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 577577Guanine nucleotide-binding protein-like 3-like proteinPRO_0000284382Add
BLAST

Proteomic databases

EPDiQ6PGG6.
MaxQBiQ6PGG6.
PaxDbiQ6PGG6.
PRIDEiQ6PGG6.

PTM databases

iPTMnetiQ6PGG6.
PhosphoSiteiQ6PGG6.

Expressioni

Gene expression databases

BgeeiQ6PGG6.
ExpressionAtlasiQ6PGG6. baseline and differential.
GenevisibleiQ6PGG6. MM.

Interactioni

Subunit structurei

Interacts with MDM2; this interaction, which occurs in the nucleoplasm, stabilizes MDM2. Indirectly interacts with TP53, via MDM2-binding. Interacts with TERF1; this interaction probably occurs in the nucleoplasm and is increased during mitosis, when the nucleolus is disassembled. This binding may promote TERF1 homodimerization. Interacts with TERT.2 Publications

Protein-protein interaction databases

BioGridi231842. 2 interactions.
IntActiQ6PGG6. 1 interaction.
STRINGi10090.ENSMUSP00000026297.

Structurei

3D structure databases

ProteinModelPortaliQ6PGG6.
SMRiQ6PGG6. Positions 119-399.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini118 – 304187CP-type GPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni9 – 2820Required for nucleolar localizationBy similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili43 – 10361Sequence analysisAdd
BLAST

Domaini

In contrast to other GTP-binding proteins, this family is characterized by a circular permutation of the GTPase motifs described by a G4-G1-G3 pattern.

Sequence similaritiesi

Belongs to the TRAFAC class YlqF/YawG GTPase family.PROSITE-ProRule annotation
Contains 1 CP-type G (guanine nucleotide-binding) domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG2484. Eukaryota.
COG1161. LUCA.
GeneTreeiENSGT00810000125472.
HOGENOMiHOG000207716.
HOVERGENiHBG051747.
InParanoidiQ6PGG6.
KOiK14538.
OMAiMESYCQD.
OrthoDBiEOG7SXW2W.
PhylomeDBiQ6PGG6.
TreeFamiTF313085.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR030378. G_CP_dom.
IPR006073. GTP_binding_domain.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01926. MMR_HSR1. 1 hit.
[Graphical view]
PRINTSiPR00326. GTP1OBG.
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS51721. G_CP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6PGG6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMKIRHKNKK PGKGSKGCKK PARQNGKKVT SRPSSAPQIV HGNDHASREA
60 70 80 90 100
ELKKKRVEEM REKQQVAREQ ERQRHRTMES YCQDVLKRQQ EFEQKEEVLQ
110 120 130 140 150
ELNMFPQLDD EATRKAYYKE FRKVVEYSDV ILEVLDARDP LGCRCFQMEE
160 170 180 190 200
TVLRAEGNKK LVLVLNKIDL VPKEIVEKWL EYLLNELPTV AFKASTQHHQ
210 220 230 240 250
VKNLTRCKVP VDQASESLLK SRACFGAENL MRVLGNYCRL GEVRGHIRVG
260 270 280 290 300
VVGLPNVGKS SLINSLKRSR ACSVGAVPGV TKFMQEVYLD KFIRLLDAPG
310 320 330 340 350
IVPGPNSEVG TILRNCIHVQ KLADPVTPVE TILQRCNLEE ISSYYGVSGF
360 370 380 390 400
QTTEHFLTAV AHRLGKKKKG GVYSQEQAAK AVLADWVSGK ISFYTLPPPT
410 420 430 440 450
HTLPTHLSAE IVKEMTEVFD IEDTEHANED TMECLAVGES DELLGDMDPQ
460 470 480 490 500
EMEVRWLHSP LVKIADAIEN RSTVYKIGNL TGYCTKPNRN QMGWPKRNVD
510 520 530 540 550
HHCPQNNRVV EVSSVDRRPM LQRILETDPL QQGQALASAL KNKKKLQKRS
560 570
DKIATKLSDS MMSMLDLSGN SDDCAGD
Length:577
Mass (Da):65,195
Last modified:July 5, 2004 - v1
Checksum:iC2F6E1FE5EED9815
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL805937 Genomic DNA. Translation: CAM14868.1.
BC057033 mRNA. Translation: AAH57033.1.
BC079653 mRNA. Translation: AAH79653.1.
CCDSiCCDS41174.1.
RefSeqiNP_001162071.1. NM_001168600.1.
NP_932778.1. NM_198110.2.
XP_006528868.1. XM_006528805.2.
UniGeneiMm.45987.

Genome annotation databases

EnsembliENSMUST00000026297; ENSMUSP00000026297; ENSMUSG00000025266.
ENSMUST00000112691; ENSMUSP00000108311; ENSMUSG00000025266.
GeneIDi237107.
KEGGimmu:237107.
UCSCiuc009uou.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL805937 Genomic DNA. Translation: CAM14868.1.
BC057033 mRNA. Translation: AAH57033.1.
BC079653 mRNA. Translation: AAH79653.1.
CCDSiCCDS41174.1.
RefSeqiNP_001162071.1. NM_001168600.1.
NP_932778.1. NM_198110.2.
XP_006528868.1. XM_006528805.2.
UniGeneiMm.45987.

3D structure databases

ProteinModelPortaliQ6PGG6.
SMRiQ6PGG6. Positions 119-399.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi231842. 2 interactions.
IntActiQ6PGG6. 1 interaction.
STRINGi10090.ENSMUSP00000026297.

PTM databases

iPTMnetiQ6PGG6.
PhosphoSiteiQ6PGG6.

Proteomic databases

EPDiQ6PGG6.
MaxQBiQ6PGG6.
PaxDbiQ6PGG6.
PRIDEiQ6PGG6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000026297; ENSMUSP00000026297; ENSMUSG00000025266.
ENSMUST00000112691; ENSMUSP00000108311; ENSMUSG00000025266.
GeneIDi237107.
KEGGimmu:237107.
UCSCiuc009uou.2. mouse.

Organism-specific databases

CTDi54552.
MGIiMGI:2448557. Gnl3l.

Phylogenomic databases

eggNOGiKOG2484. Eukaryota.
COG1161. LUCA.
GeneTreeiENSGT00810000125472.
HOGENOMiHOG000207716.
HOVERGENiHBG051747.
InParanoidiQ6PGG6.
KOiK14538.
OMAiMESYCQD.
OrthoDBiEOG7SXW2W.
PhylomeDBiQ6PGG6.
TreeFamiTF313085.

Miscellaneous databases

ChiTaRSiGnl3l. mouse.
NextBioi383226.
PROiQ6PGG6.
SOURCEiSearch...

Gene expression databases

BgeeiQ6PGG6.
ExpressionAtlasiQ6PGG6. baseline and differential.
GenevisibleiQ6PGG6. MM.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR030378. G_CP_dom.
IPR006073. GTP_binding_domain.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01926. MMR_HSR1. 1 hit.
[Graphical view]
PRINTSiPR00326. GTP1OBG.
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS51721. G_CP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Brain.
  3. "GNL3L stabilizes the TRF1 complex and promotes mitotic transition."
    Zhu Q., Meng L., Hsu J.K., Lin T., Teishima J., Tsai R.Y.
    J. Cell Biol. 185:827-839(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH TERF1 AND TERT, MUTAGENESIS OF ASN-166.
  4. "GNL3L depletion destabilizes MDM2 and induces p53-dependent G2/M arrest."
    Meng L., Hsu J.K., Tsai R.Y.
    Oncogene 30:1716-1726(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH MDM2 AND TP53, SUBCELLULAR LOCATION, MUTAGENESIS OF GLY-253.

Entry informationi

Entry nameiGNL3L_MOUSE
AccessioniPrimary (citable) accession number: Q6PGG6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 17, 2007
Last sequence update: July 5, 2004
Last modified: May 11, 2016
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.