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Protein

Mediator of RNA polymerase II transcription subunit 16

Gene

Med16

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-442533. Transcriptional Regulation of Adipocyte Differentiation in 3T3-L1 Pre-adipocytes.

Names & Taxonomyi

Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 16
Alternative name(s):
Mediator complex subunit 16
Thyroid hormone receptor-associated protein 5
Thyroid hormone receptor-associated protein complex 95 kDa component
Short name:
Trap95
Vitamin D3 receptor-interacting protein complex 92 kDa component
Short name:
DRIP92
Gene namesi
Name:Med16
Synonyms:Thrap5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:2158394. Med16.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 828828Mediator of RNA polymerase II transcription subunit 16PRO_0000051293Add
BLAST

Proteomic databases

EPDiQ6PGF3.
MaxQBiQ6PGF3.
PaxDbiQ6PGF3.
PRIDEiQ6PGF3.

PTM databases

PhosphoSiteiQ6PGF3.

Expressioni

Gene expression databases

BgeeiQ6PGF3.
CleanExiMM_MED16.

Interactioni

Subunit structurei

Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi229709. 18 interactions.
DIPiDIP-48408N.
IntActiQ6PGF3. 3 interactions.
MINTiMINT-7984911.
STRINGi10090.ENSMUSP00000129375.

Structurei

3D structure databases

ProteinModelPortaliQ6PGF3.
SMRiQ6PGF3. Positions 17-131.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati67 – 10640WD 1Add
BLAST
Repeati198 – 24043WD 2Add
BLAST
Repeati263 – 30745WD 3Add
BLAST
Repeati621 – 66242WD 4Add
BLAST
Repeati777 – 81640WD 5Add
BLAST

Sequence similaritiesi

Belongs to the Mediator complex subunit 16 family.Curated
Contains 5 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiENOG410IDXV. Eukaryota.
ENOG410ZRDM. LUCA.
HOGENOMiHOG000113526.
HOVERGENiHBG054317.
InParanoidiQ6PGF3.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
InterProiIPR021665. Mediator_Med16.
IPR011041. Quinoprot_gluc/sorb_DH.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF11635. Med16. 1 hit.
[Graphical view]
SMARTiSM00320. WD40. 2 hits.
[Graphical view]
SUPFAMiSSF50952. SSF50952. 3 hits.
SSF50978. SSF50978. 1 hit.
PROSITEiPS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6PGF3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDLAYVCEWE KWAKSTYCPS LPLACAWSCR NLIAFTTDLR NDDQDLTHMI
60 70 80 90 100
HILDTEHPWE VHSVSSGHSE AITCLEWDQS GSRLLSADAD GQIKCWSMAD
110 120 130 140 150
HLANSWESSV GSQVEGDPIV ALSWLHNGVK LALHVEKSGA SSFGEKFSRV
160 170 180 190 200
KFSPSLTLFG GKPMEGWIAV TVSGLVTVSL LKPSGQVLTS TESLCRLRGR
210 220 230 240 250
VALADIAFTG GGNIVVAAAD GSSASPVKFY KVCVSVVSEK CRIDTEILPS
260 270 280 290 300
LFMRCTTDPN RKDRFPAITH LKFLARDMSE QVLLCASSQT SSLVECWSLR
310 320 330 340 350
KEGLPVNNIF QQISPVVGDK QPMILKWRIL SATNDLDRVS AVALPKLPIS
360 370 380 390 400
LTNTDLKVAS DTQFYPGLGL ALAFQDGSVH MVHRLSLQTM AVFYSSAPRS
410 420 430 440 450
LDEPALKRPR TTCPAVHFKA MQLSWTSLAL VGIDNHGKLS MLRISPSLGH
460 470 480 490 500
PLEPKLALQH LLFLLEYCMV TGYDWWDILL HVQPGMVQSL VERLHEEYTR
510 520 530 540 550
QKPALQQVLS TRILAMKASL CKLSPCTVAR VCDYHTKLFL MAITSTLKSL
560 570 580 590 600
LRPHFLNTPD KSPGDRLAEI CAKITDVDID KVMINLKTEE FVLDMNTLQA
610 620 630 640 650
LQQLLQWVGD FVLYLLVSLP NQGSPLRPGH SFLRDGTSLG MLRELMVVIR
660 670 680 690 700
IWGLLKPSCL PVYTATSDTQ DSMSLLFRLL TKLWICCRDE GAASEPDEGL
710 720 730 740 750
VDECCLLPSQ LLVPNLDWLP ASDGLVSRLQ PKQPLRLRFG RAPTLPSSTS
760 770 780 790 800
TLQLDGLTRA PGQPKIDHLR RLHLGAYPTE ECKACTRCGC VTMLKSPNKT
810 820
TAVTQWEQRW IKNCLCGGLW RRVPLSCS
Length:828
Mass (Da):91,786
Last modified:July 27, 2011 - v2
Checksum:i6388AEF86575997D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti33 – 331I → T in AAH57056 (PubMed:15489334).Curated
Sequence conflicti390 – 3901M → L in AAH57056 (PubMed:15489334).Curated
Sequence conflicti409 – 4091P → L in AAH57056 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC151846 Genomic DNA. No translation available.
BC057056 mRNA. Translation: AAH57056.1.
RefSeqiXP_006513550.1. XM_006513487.2.
XP_006513551.1. XM_006513488.2.
UniGeneiMm.260089.

Genome annotation databases

GeneIDi216154.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC151846 Genomic DNA. No translation available.
BC057056 mRNA. Translation: AAH57056.1.
RefSeqiXP_006513550.1. XM_006513487.2.
XP_006513551.1. XM_006513488.2.
UniGeneiMm.260089.

3D structure databases

ProteinModelPortaliQ6PGF3.
SMRiQ6PGF3. Positions 17-131.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi229709. 18 interactions.
DIPiDIP-48408N.
IntActiQ6PGF3. 3 interactions.
MINTiMINT-7984911.
STRINGi10090.ENSMUSP00000129375.

PTM databases

PhosphoSiteiQ6PGF3.

Proteomic databases

EPDiQ6PGF3.
MaxQBiQ6PGF3.
PaxDbiQ6PGF3.
PRIDEiQ6PGF3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi216154.

Organism-specific databases

CTDi10025.
MGIiMGI:2158394. Med16.

Phylogenomic databases

eggNOGiENOG410IDXV. Eukaryota.
ENOG410ZRDM. LUCA.
HOGENOMiHOG000113526.
HOVERGENiHBG054317.
InParanoidiQ6PGF3.

Enzyme and pathway databases

ReactomeiR-MMU-442533. Transcriptional Regulation of Adipocyte Differentiation in 3T3-L1 Pre-adipocytes.

Miscellaneous databases

ChiTaRSiMed16. mouse.
PROiQ6PGF3.
SOURCEiSearch...

Gene expression databases

BgeeiQ6PGF3.
CleanExiMM_MED16.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
InterProiIPR021665. Mediator_Med16.
IPR011041. Quinoprot_gluc/sorb_DH.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF11635. Med16. 1 hit.
[Graphical view]
SMARTiSM00320. WD40. 2 hits.
[Graphical view]
SUPFAMiSSF50952. SSF50952. 3 hits.
SSF50978. SSF50978. 1 hit.
PROSITEiPS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6JImported.
    Tissue: BrainImported.

Entry informationi

Entry nameiMED16_MOUSE
AccessioniPrimary (citable) accession number: Q6PGF3
Secondary accession number(s): E9QM81
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 12, 2005
Last sequence update: July 27, 2011
Last modified: May 11, 2016
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.