Reviewed, UniProtKB/Swiss-Prot Q6PGD0 (APR3_MOUSE)

Names and origin

Protein names

Recommended name:
    Apoptosis-related protein 3
      Short name=APR-3

Gene names

Name:

Apr3

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	223 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	May play a critical role in inducing the cell cycle arrest By similarity.
Subcellular location	Cell membrane; Single-pass membrane protein Potential.
Sequence similarities	Contains 1 EGF-like domain.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing
Domain	EGF-like domain Signal Transmembrane
PTM	Disulfide bond
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q6PGD0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q6PGD0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-61: Missing.
     62-68: QKGTILG → MHPVSGR

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	By similarity
	Chain	26 – 223	198	Apoptosis-related protein 3		PRO_0000020753
Regions
	Topological domain	26 – 193	168	Extracellular Potential
	Transmembrane	194 – 214	21	Potential
	Topological domain	215 – 223	9	Cytoplasmic Potential
	Domain	146 – 187	42	EGF-like
Amino acid modifications
	Disulfide bond	150 ↔ 165		By similarity
	Disulfide bond	159 ↔ 175		By similarity
	Disulfide bond	177 ↔ 186		By similarity
Natural variations
	Alternative sequence	1 – 61	61	Missing in isoform 2.		VSP_014109
	Alternative sequence	62 – 68	7	QKGTILG → MHPVSGR in isoform 2.		VSP_014110
Experimental info
	Sequence conflict	23	1	A → S in BAB21981. Ref.1
	Sequence conflict	28	1	E → K in BAB21981. Ref.1
	Sequence conflict	65	1	T → P in BAB21981. Ref.1
	Sequence conflict	70	1	D → N in BAB21981. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 11, 2004. Version 2. Checksum: 1CB921F5F6D3E595	FASTA	223	23,858
	10 20 30 40 50 60 MASRESGGSR AAALLLVLGV ERALALPEIC TLCPGGMHNL SRVAAYCEDT SKLMQARCCL 70 80 90 100 110 120 NQKGTILGLD LQNCSLKDPG PNFLQAYTAI IIDLQANPLK DDLANTFRGF TQLQTLILPQ 130 140 150 160 170 180 DVPCPGGSNA WDNVTSFKDK QICQGQRDLC NSTGSPEMCP ENGSCASDGP GLLQCVCADG 190 200 210 220 FHGYKCMRQG SFSLLMFFGI LGSTTLAISI LLWGTQRRKA KAS
	Isoform 2. Checksum: AD9498698BA1B71D	FASTA	162	17,519
	10 20 30 40 50 60 MHPVSGRLDL QNCSLKDPGP NFLQAYTAII IDLQANPLKD DLANTFRGFT QLQTLILPQD 70 80 90 100 110 120 VPCPGGSNAW DNVTSFKDKQ ICQGQRDLCN STGSPEMCPE NGSCASDGPG LLQCVCADGF 130 140 150 160 HGYKCMRQGS FSLLMFFGIL GSTTLAISIL LWGTQRRKAK AS

References

[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6J. Tissue: Bone marrow, Heart and Kidney.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: C57BL/6. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AK002276 mRNA. Translation: BAB21981.1. AK151307 mRNA. Translation: BAE30289.1. AK151366 mRNA. Translation: BAE30340.1. AK169267 mRNA. Translation: BAE41028.1. BC049637 mRNA. Translation: AAH49637.1. BC057097 mRNA. Translation: AAH57097.2.
IPI	IPI00415136. IPI00471273.
RefSeq	NP_082131.2.
UniGene	Mm.28144
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	Q6PGD0.
Genome annotation databases
Ensembl	ENSMUST00000013766; ENSMUSP00000013766; ENSMUSG00000013622; Mus musculus. [Genome view]
GeneID	381629.
KEGG	mmu:381629.
UCSC	uc008www.1. mouse. uc008wwx.1. mouse.
Organism-specific databases
MGI	MGI:1918918. 0610007C21Rik.
Phylogenomic databases
HOGENOM	HBG755345.
HOVERGEN	Q6PGD0.
InParanoid	Q6PGD0.
OMA	FRGFTQL.
OrthoDB	EOG9F4VXM.
Gene expression databases
ArrayExpress	Q6PGD0.
Bgee	Q6PGD0.
CleanEx	MM_0610007C21RIK.
Genevestigator	Q6PGD0.
GermOnline	ENSMUSG00000013622. Mus musculus.
Family and domain databases
InterPro	IPR006210. EGF-like. IPR013032. EGF-like_reg_CS. IPR000742. EGF_3. [Graphical view]
SMART	SM00181. EGF. 1 hit. [Graphical view]
PROSITE	PS00022. EGF_1. 1 hit. PS01186. EGF_2. 1 hit. PS50026. EGF_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	402331.
SOURCE	Search...

Entry information

Entry name

APR3_MOUSE

Accession

Primary (citable) accession number: Q6PGD0
Secondary accession number(s): Q3UAH4, Q810Q3, Q9DD14

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2005
Last sequence update:	October 11, 2004
Last modified:	December 15, 2009
This is version 52 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)