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Protein

All-trans retinoic acid-induced differentiation factor

Gene

Atraid

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Promotes osteoblast cell differentiation and terminal mineralization. Plays a role in inducing the cell cycle arrest via inhibiting CCND1 expression in all-trans-retinoic acid (ATRA) signal pathway (By similarity).By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Differentiation

Names & Taxonomyi

Protein namesi
Recommended name:
All-trans retinoic acid-induced differentiation factor
Alternative name(s):
Apoptosis-related protein 3
Short name:
APR-3
Gene namesi
Name:Atraid
Synonyms:Apr3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1918918. Atraid.

Subcellular locationi

  • Nucleus envelope By similarity
  • Cell membrane By similarity; Single-pass membrane protein By similarity

  • Note: Colocalizes with NELL1 on the nuclear envelope and the perinuclear region.By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini26 – 193ExtracellularSequence analysisAdd BLAST168
Transmembranei194 – 214HelicalSequence analysisAdd BLAST21
Topological domaini215 – 223CytoplasmicSequence analysis9

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 25By similarityAdd BLAST25
ChainiPRO_000002075326 – 223All-trans retinoic acid-induced differentiation factorAdd BLAST198

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi150 ↔ 165PROSITE-ProRule annotation
Disulfide bondi159 ↔ 175PROSITE-ProRule annotation
Disulfide bondi177 ↔ 186PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiQ6PGD0.
PRIDEiQ6PGD0.

PTM databases

iPTMnetiQ6PGD0.
PhosphoSitePlusiQ6PGD0.

Expressioni

Gene expression databases

BgeeiENSMUSG00000013622.
CleanExiMM_0610007C21RIK.
ExpressionAtlasiQ6PGD0. baseline and differential.
GenevisibleiQ6PGD0. MM.

Interactioni

Subunit structurei

Interacts with NELL1; the interaction promotes osteoblastic differentiation and mineralization.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000013766.

Structurei

3D structure databases

ProteinModelPortaliQ6PGD0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini146 – 187EGF-likePROSITE-ProRule annotationAdd BLAST42

Sequence similaritiesi

Contains 1 EGF-like domain.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IYNU. Eukaryota.
ENOG4111PJW. LUCA.
GeneTreeiENSGT00390000017252.
HOGENOMiHOG000010110.
HOVERGENiHBG054041.
InParanoidiQ6PGD0.
OMAiGINAWNT.
OrthoDBiEOG091G0PFL.
PhylomeDBiQ6PGD0.
TreeFamiTF335766.

Family and domain databases

Gene3Di3.80.10.10. 1 hit.
InterProiIPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR032675. L_dom-like.
[Graphical view]
PROSITEiPS00022. EGF_1. 1 hit.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6PGD0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASRESGGSR AAALLLVLGV ERALALPEIC TLCPGGMHNL SRVAAYCEDT
60 70 80 90 100
SKLMQARCCL NQKGTILGLD LQNCSLKDPG PNFLQAYTAI IIDLQANPLK
110 120 130 140 150
DDLANTFRGF TQLQTLILPQ DVPCPGGSNA WDNVTSFKDK QICQGQRDLC
160 170 180 190 200
NSTGSPEMCP ENGSCASDGP GLLQCVCADG FHGYKCMRQG SFSLLMFFGI
210 220
LGSTTLAISI LLWGTQRRKA KAS
Length:223
Mass (Da):23,858
Last modified:October 11, 2004 - v2
Checksum:i1CB921F5F6D3E595
GO
Isoform 2 (identifier: Q6PGD0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     37-223: Missing.

Note: No experimental confirmation available. May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.
Show »
Length:36
Mass (Da):3,583
Checksum:i12001B7098C55C61
GO

Sequence cautioni

The sequence AAH49637 differs from that shown. Reason: Erroneous translation. Wrong choice of CDS.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti23A → S in BAB21981 (PubMed:16141072).Curated1
Sequence conflicti28E → K in BAB21981 (PubMed:16141072).Curated1
Sequence conflicti65T → P in BAB21981 (PubMed:16141072).Curated1
Sequence conflicti70D → N in BAB21981 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_03997037 – 223Missing in isoform 2. 1 PublicationAdd BLAST187

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK002276 mRNA. Translation: BAB21981.1.
AK151307 mRNA. Translation: BAE30289.1.
AK151366 mRNA. Translation: BAE30340.1.
AK169267 mRNA. Translation: BAE41028.1.
BC049637 mRNA. Translation: AAH49637.1. Sequence problems.
BC057097 mRNA. Translation: AAH57097.2.
CCDSiCCDS19170.1. [Q6PGD0-1]
RefSeqiNP_082131.2. NM_027855.4. [Q6PGD0-1]
NP_997635.1. NM_212470.3.
UniGeneiMm.28144.

Genome annotation databases

EnsembliENSMUST00000013766; ENSMUSP00000013766; ENSMUSG00000013622. [Q6PGD0-1]
ENSMUST00000200942; ENSMUSP00000144431; ENSMUSG00000013622. [Q6PGD0-2]
GeneIDi381629.
KEGGimmu:381629.
UCSCiuc008www.3. mouse. [Q6PGD0-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK002276 mRNA. Translation: BAB21981.1.
AK151307 mRNA. Translation: BAE30289.1.
AK151366 mRNA. Translation: BAE30340.1.
AK169267 mRNA. Translation: BAE41028.1.
BC049637 mRNA. Translation: AAH49637.1. Sequence problems.
BC057097 mRNA. Translation: AAH57097.2.
CCDSiCCDS19170.1. [Q6PGD0-1]
RefSeqiNP_082131.2. NM_027855.4. [Q6PGD0-1]
NP_997635.1. NM_212470.3.
UniGeneiMm.28144.

3D structure databases

ProteinModelPortaliQ6PGD0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000013766.

PTM databases

iPTMnetiQ6PGD0.
PhosphoSitePlusiQ6PGD0.

Proteomic databases

PaxDbiQ6PGD0.
PRIDEiQ6PGD0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000013766; ENSMUSP00000013766; ENSMUSG00000013622. [Q6PGD0-1]
ENSMUST00000200942; ENSMUSP00000144431; ENSMUSG00000013622. [Q6PGD0-2]
GeneIDi381629.
KEGGimmu:381629.
UCSCiuc008www.3. mouse. [Q6PGD0-1]

Organism-specific databases

CTDi51374.
MGIiMGI:1918918. Atraid.

Phylogenomic databases

eggNOGiENOG410IYNU. Eukaryota.
ENOG4111PJW. LUCA.
GeneTreeiENSGT00390000017252.
HOGENOMiHOG000010110.
HOVERGENiHBG054041.
InParanoidiQ6PGD0.
OMAiGINAWNT.
OrthoDBiEOG091G0PFL.
PhylomeDBiQ6PGD0.
TreeFamiTF335766.

Miscellaneous databases

PROiQ6PGD0.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000013622.
CleanExiMM_0610007C21RIK.
ExpressionAtlasiQ6PGD0. baseline and differential.
GenevisibleiQ6PGD0. MM.

Family and domain databases

Gene3Di3.80.10.10. 1 hit.
InterProiIPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR032675. L_dom-like.
[Graphical view]
PROSITEiPS00022. EGF_1. 1 hit.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiARAID_MOUSE
AccessioniPrimary (citable) accession number: Q6PGD0
Secondary accession number(s): Q3UAH4, Q810Q3, Q9DD14
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: October 11, 2004
Last modified: November 30, 2016
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.