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Protein

ATP-dependent RNA helicase Dhx29

Gene

Dhx29

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

ATP-binding RNA helicase involved in translation initiation. Part of the 43S preinitiation complex that is required for efficient initiation on mammalian mRNAs with structured 5'-UTRs by promoting efficient NTPase-dependent 48S complex formation. Specifically binds to the 40S ribosome near the mRNA entrance. Does not possess a processive helicase activity (By similarity).By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi594 – 6018ATPPROSITE-ProRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase, Initiation factor

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent RNA helicase Dhx29 (EC:3.6.4.13)
Alternative name(s):
DEAH box protein 29
Gene namesi
Name:Dhx29
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:2145374. Dhx29.

Subcellular locationi

GO - Cellular componenti

  • eukaryotic 43S preinitiation complex Source: UniProtKB
  • mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 13651365ATP-dependent RNA helicase Dhx29PRO_0000245536Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei69 – 691PhosphoserineBy similarity
Modified residuei190 – 1901PhosphoserineBy similarity
Modified residuei198 – 1981PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ6PGC1.
MaxQBiQ6PGC1.
PaxDbiQ6PGC1.
PRIDEiQ6PGC1.

PTM databases

PhosphoSiteiQ6PGC1.

Expressioni

Gene expression databases

BgeeiQ6PGC1.
CleanExiMM_DHX29.
GenevisibleiQ6PGC1. MM.

Interactioni

Subunit structurei

Part of the 43S preinitiation complex that contains at least Met-tRNA, EIF1, EIF1A (EIF1AX or EIF1AY), EIF2S1, EIF2S2, EIF2S3, EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L, EIF3M, DHX29 and the 40S ribosomal subunit.By similarity

Protein-protein interaction databases

IntActiQ6PGC1. 1 interaction.
MINTiMINT-4112300.
STRINGi10090.ENSMUSP00000035244.

Structurei

3D structure databases

ProteinModelPortaliQ6PGC1.
SMRiQ6PGC1. Positions 519-753, 1042-1288.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini581 – 754174Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini848 – 1025178Helicase C-terminalPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili264 – 30946Sequence analysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi701 – 7044DEAH box

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi10 – 5344Ala-richAdd
BLAST
Compositional biasi499 – 5079Poly-Gln
Compositional biasi788 – 7925Poly-Glu

Sequence similaritiesi

Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0920. Eukaryota.
COG1643. LUCA.
GeneTreeiENSGT00760000119189.
HOGENOMiHOG000247063.
HOVERGENiHBG081436.
InParanoidiQ6PGC1.
KOiK18995.
OMAiQHSENKR.
OrthoDBiEOG7SV0TS.
PhylomeDBiQ6PGC1.
TreeFamiTF324744.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR002464. DNA/RNA_helicase_DEAH_CS.
IPR011709. DUF1605.
IPR007502. Helicase-assoc_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF04408. HA2. 1 hit.
PF00271. Helicase_C. 1 hit.
PF07717. OB_NTP_bind. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00847. HA2. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS00690. DEAH_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6PGC1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGGKNKKHKA PGAAAMRAAV SASRARSAEA GAVGEAQSKK PVARPAPAVP
60 70 80 90 100
TGAREPRVKQ GPKIYSFNSA NDSGGSANLD KSILKVVINN KLEQRIIGVI
110 120 130 140 150
NEHKKQNSDR GAISGRLSAK KLQDLYMALQ AFSFKTKDIE DAMTNTLLHG
160 170 180 190 200
GDLHSALDWL CLNLSDDALP EGFSQEFEEQ QPKSRPKFQS VQIQATLSPP
210 220 230 240 250
QQTKTKRQEE DPKIKPKKEE TTVEVNMKEW ILRYAEQQDE EEKGEGSKGL
260 270 280 290 300
EEEEKFDPNQ RYLNLAARLL DAKEQAAAFK LEKNKQGQKE AQEKIRKFQR
310 320 330 340 350
EMETLEDHPI FNPAIKISHQ QNEKKKPAPA TEAESALNLN LFEKSAAATE
360 370 380 390 400
EEKGKKKEPH DVRNFDYTAR SWTGKSPKQF LIDWVRKNLP KSPNPSFEKV
410 420 430 440 450
AVGRYWKCRV RVVRSEDDVL VVCPTILTED GMQAQHLGAT LALYRLVKGQ
460 470 480 490 500
SVHQLLPPTY RDVWLEWSDE EKKREELNKM ETNKPRDLFI AKLLNKLKQQ
510 520 530 540 550
QQQQQQQRPE SEKGGSEDPE ESWENLVSDE DLAALSLEPT SAEDLAPVRS
560 570 580 590 600
LFRRLQSTPK YQRLLKERQQ LPVFKHRDSI VETLKRHRVV VVAGETGSGK
610 620 630 640 650
STQVPHFLLE DLLLDECGAR KCNIVCTQPR RISAVSLATR VCEELGCESG
660 670 680 690 700
PGGRNSLCGY QIRMESRASE STRLLYCTTG VLLRKLQEDG LLADVSHVIV
710 720 730 740 750
DEVHERSVQS DFLLVILKEI LQKRSDLHLI LMSATVDSDK FSTYFTHCPI
760 770 780 790 800
LRISGRSYPV EVFHLEDIVE ETGFVLEKDS EYCQKFLEEE EEITINVTSK
810 820 830 840 850
AGGVKKYQEY IPVQSGASPE LNPFYQKYSS RTQHAILYMN PHKINLDLIL
860 870 880 890 900
ELLVYLDKSP QFRNIEGAVL IFLPGLAHIQ QLYDLLSSDR RFYSERYQVI
910 920 930 940 950
ALHSVLSTQD QAAAFMFPPP GVRKIVLATN IAETGITIPD VVFVIDTGRT
960 970 980 990 1000
KENKYHESSQ MSSLVETFVS KASALQRQGR AGRVRDGFCF RLYTRERFEG
1010 1020 1030 1040 1050
FLDYSVPEIL RVPLEELCLH IMKCDLGSPE DFLSKALDPP QLQVISNAMN
1060 1070 1080 1090 1100
LLRKIGACEP NEPKLTPLGQ HLAALPVNVK IGKMLIFGAI FGCLEPVATL
1110 1120 1130 1140 1150
AAVMTEKSPF ITPIGRKDEA DLAKSSLAVA DSDHLTIYNA YLGWKKAQQE
1160 1170 1180 1190 1200
GGFRSEISYC QRNFLNRTSL LTLEDVKQEL MKLVKAAGFS SSPSWEGRKG
1210 1220 1230 1240 1250
PQTLSFQDIA LLKAVLAAGL YDSVGKIMCT KSVDVTEKLA CMVETAQGKA
1260 1270 1280 1290 1300
QVHPSSVNRD LQTYGWLLYQ EKVRYTRVYL RETTLITPFP VLLFGGDIEV
1310 1320 1330 1340 1350
QHRERLLSVD GWIYFQAPVK IAVIFKQLRV LIDSVLRKKL ENPKMSLEND
1360
KILQIITELI KTENN
Length:1,365
Mass (Da):153,975
Last modified:July 5, 2004 - v1
Checksum:i4143A705CB092280
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC057112 mRNA. Translation: AAH57112.1.
BC082319 mRNA. Translation: AAH82319.1.
AK028342 mRNA. Translation: BAC25894.1.
AK042066 mRNA. Translation: BAC31148.2.
AK042497 mRNA. Translation: BAC31274.2.
AK049530 mRNA. Translation: BAC33796.1.
CCDSiCCDS26779.1.
RefSeqiNP_766182.2. NM_172594.2.
UniGeneiMm.35094.
Mm.471395.

Genome annotation databases

EnsembliENSMUST00000038574; ENSMUSP00000035244; ENSMUSG00000042426.
GeneIDi218629.
KEGGimmu:218629.
UCSCiuc007rwx.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC057112 mRNA. Translation: AAH57112.1.
BC082319 mRNA. Translation: AAH82319.1.
AK028342 mRNA. Translation: BAC25894.1.
AK042066 mRNA. Translation: BAC31148.2.
AK042497 mRNA. Translation: BAC31274.2.
AK049530 mRNA. Translation: BAC33796.1.
CCDSiCCDS26779.1.
RefSeqiNP_766182.2. NM_172594.2.
UniGeneiMm.35094.
Mm.471395.

3D structure databases

ProteinModelPortaliQ6PGC1.
SMRiQ6PGC1. Positions 519-753, 1042-1288.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ6PGC1. 1 interaction.
MINTiMINT-4112300.
STRINGi10090.ENSMUSP00000035244.

PTM databases

PhosphoSiteiQ6PGC1.

Proteomic databases

EPDiQ6PGC1.
MaxQBiQ6PGC1.
PaxDbiQ6PGC1.
PRIDEiQ6PGC1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000038574; ENSMUSP00000035244; ENSMUSG00000042426.
GeneIDi218629.
KEGGimmu:218629.
UCSCiuc007rwx.1. mouse.

Organism-specific databases

CTDi54505.
MGIiMGI:2145374. Dhx29.

Phylogenomic databases

eggNOGiKOG0920. Eukaryota.
COG1643. LUCA.
GeneTreeiENSGT00760000119189.
HOGENOMiHOG000247063.
HOVERGENiHBG081436.
InParanoidiQ6PGC1.
KOiK18995.
OMAiQHSENKR.
OrthoDBiEOG7SV0TS.
PhylomeDBiQ6PGC1.
TreeFamiTF324744.

Miscellaneous databases

NextBioi376359.
PROiQ6PGC1.
SOURCEiSearch...

Gene expression databases

BgeeiQ6PGC1.
CleanExiMM_DHX29.
GenevisibleiQ6PGC1. MM.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR002464. DNA/RNA_helicase_DEAH_CS.
IPR011709. DUF1605.
IPR007502. Helicase-assoc_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF04408. HA2. 1 hit.
PF00271. Helicase_C. 1 hit.
PF07717. OB_NTP_bind. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00847. HA2. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS00690. DEAH_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Brain.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-469 AND 745-1365.
    Strain: C57BL/6J.
    Tissue: Embryo and Thymus.
  3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-198, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiDHX29_MOUSE
AccessioniPrimary (citable) accession number: Q6PGC1
Secondary accession number(s): Q8BQJ4
, Q8BT01, Q8C9B7, Q8C9H9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: July 5, 2004
Last modified: May 11, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.