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Q6PG34 (CDKAL_DANRE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Threonylcarbamoyladenosine tRNA methylthiotransferase

EC=2.-.-.-
Alternative name(s):
CDK5 regulatory subunit-associated protein 1-like 1
tRNA-t(6)A37 methylthiotransferase
Gene names
Name:cdkal1
ORF Names:zgc:65864
OrganismDanio rerio (Zebrafish) (Brachydanio rerio) [Reference proteome]
Taxonomic identifier7955 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio

Protein attributes

Sequence length547 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the methylthiolation of N6-threonylcarbamoyladenosine (t6A), leading to the formation of 2-methylthio-N6-threonylcarbamoyladenosine (ms2t6A) at position 37 in tRNAs that read codons beginning with adenine By similarity.

Cofactor

Binds 2 4Fe-4S clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Subcellular location

Endoplasmic reticulum membrane; Single-pass membrane protein By similarity.

Sequence similarities

Belongs to the methylthiotransferase family. CDKAL1 subfamily.

Contains 1 MTTase N-terminal domain.

Contains 1 TRAM domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 547547Threonylcarbamoyladenosine tRNA methylthiotransferase
PRO_0000298672

Regions

Transmembrane527 – 54721Helical; Potential
Domain59 – 167109MTTase N-terminal
Domain426 – 48863TRAM

Sites

Metal binding681Iron-sulfur (4Fe-4S) By similarity
Metal binding1041Iron-sulfur (4Fe-4S) By similarity
Metal binding1331Iron-sulfur (4Fe-4S) By similarity
Metal binding2091Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Metal binding2131Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Metal binding2161Iron-sulfur (4Fe-4S-S-AdoMet) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q6PG34 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 0642C4290129E46C

FASTA54761,938
        10         20         30         40         50         60 
MMALVCDTAV DDIEDMVRIT DVTPLQRQAA RKSVVPRARK HKQETGEQMQ TDSVIPGMQK 

        70         80         90        100        110        120 
VWLKTWGCSH NSSDGEYMAG QLAVAGYQIT EDSSDADLWL LNSCTVKSPA EDHFRNAIRK 

       130        140        150        160        170        180 
AQEQNKKVVL AGCVPQAQPR MDYIKDLSII GVQQIDRVVE VVDEAIKGHS VRLLGQKKEK 

       190        200        210        220        230        240 
GKRLGGARLD LPKIRKNPLI EIISINTGCL NACTYCKTKH ARGDLASYPV EELVERVRQS 

       250        260        270        280        290        300 
FQEGVCEIWL TSEDTGAYGR DIGSDLPTLL WRLVEEIPEG AMLRLGMTNP PYILEHLEEM 

       310        320        330        340        350        360 
SRILQHPRVF SFLHVPLQSA SDSVLMEMRR EYCCADFTHL VDYLKERVPG ITIATDIICG 

       370        380        390        400        410        420 
FPGETDEDFE QTLALVRRYR FPSLFINQFY PRPGTPAALM QQLPAHVKKQ RTKELSALFH 

       430        440        450        460        470        480 
SYRPYDHKMG EQQQVLVTEE SFDSQYYVAH NKFYEQVLVP KRPEYLGKMV QVEVYECGKH 

       490        500        510        520        530        540 
YMKGRPLEEA PLRTAYTTAP LLKGQVSGHT QAGVCEPQCW MPDGMRILAV VLLLSAVLLA 


LLMEKLL 

« Hide

References

[1]NIH - Zebrafish Gene Collection (ZGC) project
Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Embryo.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC057248 mRNA. Translation: AAH57248.1.
RefSeqNP_956921.1. NM_200627.1.
UniGeneDr.118384.

3D structure databases

ProteinModelPortalQ6PG34.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ6PG34.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID393600.
KEGGdre:393600.

Organism-specific databases

CTD54901.
ZFINZDB-GENE-040426-1443. cdkal1.

Phylogenomic databases

HOGENOMHOG000224768.
HOVERGENHBG097712.
InParanoidQ6PG34.
KOK15865.
PhylomeDBQ6PG34.

Family and domain databases

Gene3D3.80.30.20. 1 hit.
InterProIPR006638. Elp3/MiaB/NifB.
IPR023970. MeThioTfrase/rSAM.
IPR005839. Methylthiotransferase.
IPR020612. Methylthiotransferase_CS.
IPR013848. Methylthiotransferase_N.
IPR006466. MiaB-like_B.
IPR007197. rSAM.
IPR023404. rSAM_horseshoe.
IPR002792. TRAM_dom.
[Graphical view]
PANTHERPTHR11918. PTHR11918. 1 hit.
PfamPF04055. Radical_SAM. 1 hit.
PF00919. UPF0004. 1 hit.
[Graphical view]
SMARTSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR01578. MiaB-like-B. 1 hit.
TIGR00089. TIGR00089. 1 hit.
PROSITEPS51449. MTTASE_N. 1 hit.
PS01278. MTTASE_RADICAL. 1 hit.
PS50926. TRAM. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20814618.
PROQ6PG34.

Entry information

Entry nameCDKAL_DANRE
AccessionPrimary (citable) accession number: Q6PG34
Entry history
Integrated into UniProtKB/Swiss-Prot: August 21, 2007
Last sequence update: July 5, 2004
Last modified: April 16, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families