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Q6PG34

- CDKAL_DANRE

UniProt

Q6PG34 - CDKAL_DANRE

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Protein

Threonylcarbamoyladenosine tRNA methylthiotransferase

Gene

cdkal1

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Catalyzes the methylthiolation of N6-threonylcarbamoyladenosine (t6A), leading to the formation of 2-methylthio-N6-threonylcarbamoyladenosine (ms2t6A) at position 37 in tRNAs that read codons beginning with adenine.By similarity

Catalytic activityi

N(6)-L-threonylcarbamoyladenine(37) in tRNA + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = 2-methylthio-N(6)-L-threonylcarbamoyladenine(37) in tRNA + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine.

Cofactori

Binds 2 4Fe-4S clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi68 – 681Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation
Metal bindingi104 – 1041Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation
Metal bindingi133 – 1331Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation
Metal bindingi209 – 2091Iron-sulfur (4Fe-4S-S-AdoMet)PROSITE-ProRule annotation
Metal bindingi213 – 2131Iron-sulfur (4Fe-4S-S-AdoMet)PROSITE-ProRule annotation
Metal bindingi216 – 2161Iron-sulfur (4Fe-4S-S-AdoMet)PROSITE-ProRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. transferase activity Source: UniProtKB-KW

GO - Biological processi

  1. RNA modification Source: InterPro
  2. tRNA processing Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Threonylcarbamoyladenosine tRNA methylthiotransferase (EC:2.8.4.5)
Alternative name(s):
CDK5 regulatory subunit-associated protein 1-like 1
tRNA-t(6)A37 methylthiotransferase
Gene namesi
Name:cdkal1
ORF Names:zgc:65864
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
ProteomesiUP000000437: Unplaced

Organism-specific databases

ZFINiZDB-GENE-040426-1443. cdkal1.

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB-KW
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 547547Threonylcarbamoyladenosine tRNA methylthiotransferasePRO_0000298672Add
BLAST

Proteomic databases

PRIDEiQ6PG34.

Structurei

3D structure databases

ProteinModelPortaliQ6PG34.
ModBaseiSearch...
MobiDBiSearch...

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei527 – 54721HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini59 – 167109MTTase N-terminalPROSITE-ProRule annotationAdd
BLAST
Domaini426 – 48863TRAMPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 MTTase N-terminal domain.PROSITE-ProRule annotation
Contains 1 TRAM domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000224768.
HOVERGENiHBG097712.
InParanoidiQ6PG34.
KOiK15865.
PhylomeDBiQ6PG34.

Family and domain databases

Gene3Di3.80.30.20. 1 hit.
InterProiIPR006638. Elp3/MiaB/NifB.
IPR023970. MeThioTfrase/rSAM.
IPR005839. Methylthiotransferase.
IPR020612. Methylthiotransferase_CS.
IPR013848. Methylthiotransferase_N.
IPR006466. MiaB-like_B.
IPR007197. rSAM.
IPR023404. rSAM_horseshoe.
IPR002792. TRAM_dom.
[Graphical view]
PANTHERiPTHR11918. PTHR11918. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
PF00919. UPF0004. 1 hit.
[Graphical view]
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01578. MiaB-like-B. 1 hit.
TIGR00089. TIGR00089. 1 hit.
PROSITEiPS51449. MTTASE_N. 1 hit.
PS01278. MTTASE_RADICAL. 1 hit.
PS50926. TRAM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6PG34-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MMALVCDTAV DDIEDMVRIT DVTPLQRQAA RKSVVPRARK HKQETGEQMQ
60 70 80 90 100
TDSVIPGMQK VWLKTWGCSH NSSDGEYMAG QLAVAGYQIT EDSSDADLWL
110 120 130 140 150
LNSCTVKSPA EDHFRNAIRK AQEQNKKVVL AGCVPQAQPR MDYIKDLSII
160 170 180 190 200
GVQQIDRVVE VVDEAIKGHS VRLLGQKKEK GKRLGGARLD LPKIRKNPLI
210 220 230 240 250
EIISINTGCL NACTYCKTKH ARGDLASYPV EELVERVRQS FQEGVCEIWL
260 270 280 290 300
TSEDTGAYGR DIGSDLPTLL WRLVEEIPEG AMLRLGMTNP PYILEHLEEM
310 320 330 340 350
SRILQHPRVF SFLHVPLQSA SDSVLMEMRR EYCCADFTHL VDYLKERVPG
360 370 380 390 400
ITIATDIICG FPGETDEDFE QTLALVRRYR FPSLFINQFY PRPGTPAALM
410 420 430 440 450
QQLPAHVKKQ RTKELSALFH SYRPYDHKMG EQQQVLVTEE SFDSQYYVAH
460 470 480 490 500
NKFYEQVLVP KRPEYLGKMV QVEVYECGKH YMKGRPLEEA PLRTAYTTAP
510 520 530 540
LLKGQVSGHT QAGVCEPQCW MPDGMRILAV VLLLSAVLLA LLMEKLL
Length:547
Mass (Da):61,938
Last modified:July 5, 2004 - v1
Checksum:i0642C4290129E46C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC057248 mRNA. Translation: AAH57248.1.
RefSeqiNP_956921.1. NM_200627.1.
UniGeneiDr.118384.

Genome annotation databases

GeneIDi393600.
KEGGidre:393600.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC057248 mRNA. Translation: AAH57248.1 .
RefSeqi NP_956921.1. NM_200627.1.
UniGenei Dr.118384.

3D structure databases

ProteinModelPortali Q6PG34.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi Q6PG34.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 393600.
KEGGi dre:393600.

Organism-specific databases

CTDi 54901.
ZFINi ZDB-GENE-040426-1443. cdkal1.

Phylogenomic databases

HOGENOMi HOG000224768.
HOVERGENi HBG097712.
InParanoidi Q6PG34.
KOi K15865.
PhylomeDBi Q6PG34.

Miscellaneous databases

NextBioi 20814618.
PROi Q6PG34.

Family and domain databases

Gene3Di 3.80.30.20. 1 hit.
InterProi IPR006638. Elp3/MiaB/NifB.
IPR023970. MeThioTfrase/rSAM.
IPR005839. Methylthiotransferase.
IPR020612. Methylthiotransferase_CS.
IPR013848. Methylthiotransferase_N.
IPR006466. MiaB-like_B.
IPR007197. rSAM.
IPR023404. rSAM_horseshoe.
IPR002792. TRAM_dom.
[Graphical view ]
PANTHERi PTHR11918. PTHR11918. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
PF00919. UPF0004. 1 hit.
[Graphical view ]
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01578. MiaB-like-B. 1 hit.
TIGR00089. TIGR00089. 1 hit.
PROSITEi PS51449. MTTASE_N. 1 hit.
PS01278. MTTASE_RADICAL. 1 hit.
PS50926. TRAM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. NIH - Zebrafish Gene Collection (ZGC) project
    Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Embryo.

Entry informationi

Entry nameiCDKAL_DANRE
AccessioniPrimary (citable) accession number: Q6PG34
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 21, 2007
Last sequence update: July 5, 2004
Last modified: October 29, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3