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Protein

Holliday junction recognition protein

Gene

Hjurp

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Centromeric protein that plays a central role in the incorporation and maintenance of histone H3-like variant CENPA at centromeres. Acts as a specific chaperone for CENPA and is required for the incorporation of newly synthesized CENPA molecules into nucleosomes at replicated centromeres. Prevents CENPA-H4 tetramerization and prevents premature DNA binding by the CENPA-H4 tetramer. Directly binds Holliday junctions.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

Cell cycle

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Holliday junction recognition protein
Alternative name(s):
Fetal liver expressing gene 1 protein homolog
Short name:
mFleg1
Gene namesi
Name:Hjurp
Synonyms:Fleg1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:2685821. Hjurp.

Subcellular locationi

  • Nucleusnucleolus
  • Chromosomecentromere

  • Note: Localizes in centromeres during late telophase and early G1, when CENPA nucleosomes are assembled. Localizes to nucleolus during S phase, nucleolus site being often related to storage (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003781541 – 667Holliday junction recognition proteinAdd BLAST667

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei169PhosphoserineBy similarity1
Modified residuei185PhosphoserineBy similarity1
Modified residuei195PhosphoserineBy similarity1
Modified residuei388PhosphoserineCombined sources1
Modified residuei424PhosphoserineBy similarity1
Modified residuei449PhosphoserineBy similarity1
Modified residuei462PhosphoserineBy similarity1
Modified residuei567PhosphoserineBy similarity1
Modified residuei613PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ6PG16.
PRIDEiQ6PG16.

PTM databases

iPTMnetiQ6PG16.
PhosphoSitePlusiQ6PG16.

Interactioni

Subunit structurei

Interacts with CENPA (via CATD domain); the interaction is direct and specific for CENPA since it does not interact with H3.1- or H3.3-containing nucleosomes. Heterotrimer composed of HJURP, CENPA and histone H4, where HJURP interacts with the dimer formed by CENPA and histone H4 and prevents tetramerization of CENPA and H4. Identified in a centromere complex containing histones H2A, H2B and H4, and at least CENPA, CENPB, CENPC, CENPT, CENPN, HJURP, SUPT16H, SSRP1 and RSF1. Interacts with 14-3-3 family members in a phosphorylation-dependent manner. Interacts with MSH5 and NBN.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000054263.

Structurei

3D structure databases

ProteinModelPortaliQ6PG16.
SMRiQ6PG16.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

eggNOGiENOG410IW66. Eukaryota.
ENOG4111C4D. LUCA.
HOGENOMiHOG000112909.
InParanoidiQ6PG16.
PhylomeDBiQ6PG16.

Family and domain databases

InterProiIPR021052. HJURP.
IPR022102. HJURP_C.
IPR018465. Scm3/HJURP.
[Graphical view]
PfamiPF12347. HJURP_C. 2 hits.
PF12346. HJURP_mid. 1 hit.
PF10384. Scm3. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6PG16-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MESMGRQDRR LHQQLKESSS RFQTLMKRLI AKYNQPFEDD PLVEMRTLTY
60 70 80 90 100
ETPQGLRVWG GKLMKKEDKE YTQVIDRLNG QAPEGDSESS GADTSLEENW
110 120 130 140 150
PSCSSAMREA SGDPRQRQPA VPGNTLETDL RRKYLTQVDI LPQDEEYFKN
160 170 180 190 200
AEKRGGKDTV MTWVPSVTSS VTPASGCQDA ISAKSSGGPE VSALSSRGQG
210 220 230 240 250
PSYPCPADMA IVARSDGLSL LGTSSNSVSS QSFEVDDLCN VTISDLYEGM
260 270 280 290 300
MHSMSRLLRS KPSCIISTKT YINQSWKLRR RPSRKQGLHK NRTHCPRSKP
310 320 330 340 350
SQRSARKGPA SCSEPGKEAG ILRDYGNLLH VAPHKTGLEL KSVSLEGSKR
360 370 380 390 400
QVHKSSPAWK ELQMMPQKDL DLNRERENRV MTLQWLISPV KVVPRPRMLP
410 420 430 440 450
SQVEKWYREI KIKFDKLHQE YCLSSGKQPR LTDPTESWAV DVYRSGSKSP
460 470 480 490 500
GSRQDVETCR PSSPFGREKT ERPGEALEDL RGNGKSVKTK SCLLRSCPSP
510 520 530 540 550
EGSPSRSPSH SQLSSGLQEH NSEPTGKAVW PSTAISAPSI GSPGCGKDNY
560 570 580 590 600
YELKKEFNRL YQKYCLSPQR AKVTSCGRVS PMKAAAALPC QSEHLKRLNP
610 620 630 640 650
DSPQQSSQKR SISPGCHRRV LQDSTAQTAS TLVRDSWLPT KRCKLSYPVA
660
CAHQAKFHDT SGASGWP
Length:667
Mass (Da):74,199
Last modified:July 5, 2004 - v1
Checksum:i258747B28A1123C9
GO
Isoform 2 (identifier: Q6PG16-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     286-302: Missing.

Note: No experimental confirmation available.
Show »
Length:650
Mass (Da):72,243
Checksum:i2F5259C4C49B91B1
GO
Isoform 3 (identifier: Q6PG16-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     74-149: Missing.

Show »
Length:591
Mass (Da):65,763
Checksum:iC0F34D5D7552D7A3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti12H → Q in BAC31301 (PubMed:16141072).Curated1
Sequence conflicti12H → Q in AAI31920 (PubMed:15489334).Curated1
Sequence conflicti16K → N in BAC31301 (PubMed:16141072).Curated1
Sequence conflicti16K → N in AAI31920 (PubMed:15489334).Curated1
Sequence conflicti19S → R in BAC31301 (PubMed:16141072).Curated1
Sequence conflicti19S → R in AAI31920 (PubMed:15489334).Curated1
Sequence conflicti46R → T in BAC31301 (PubMed:16141072).Curated1
Sequence conflicti46R → T in AAI31920 (PubMed:15489334).Curated1
Sequence conflicti190E → G in BAC31301 (PubMed:16141072).Curated1
Sequence conflicti190E → G in AAI31920 (PubMed:15489334).Curated1
Sequence conflicti245D → N in BAC31301 (PubMed:16141072).Curated1
Sequence conflicti245D → N in AAI31920 (PubMed:15489334).Curated1
Sequence conflicti262P → L in BAC31301 (PubMed:16141072).Curated1
Sequence conflicti262P → L in AAI31920 (PubMed:15489334).Curated1
Sequence conflicti315P → H in BAC31301 (PubMed:16141072).Curated1
Sequence conflicti315P → H in AAI31920 (PubMed:15489334).Curated1
Sequence conflicti355S → F in BAC31301 (PubMed:16141072).Curated1
Sequence conflicti355S → F in AAI31920 (PubMed:15489334).Curated1
Sequence conflicti380V → G in BAC31301 (PubMed:16141072).Curated1
Sequence conflicti380V → G in AAI31920 (PubMed:15489334).Curated1
Sequence conflicti539S → C in BAC31301 (PubMed:16141072).Curated1
Sequence conflicti539S → C in AAI31920 (PubMed:15489334).Curated1
Sequence conflicti551Y → D in BAD36742 (Ref. 1) Curated1
Sequence conflicti551Y → D in BAC31301 (PubMed:16141072).Curated1
Sequence conflicti551Y → D in AAI31920 (PubMed:15489334).Curated1
Sequence conflicti626A → P in BAC31301 (PubMed:16141072).Curated1
Sequence conflicti626A → P in AAI31920 (PubMed:15489334).Curated1
Sequence conflicti635 – 636DS → EP in BAC31301 (PubMed:16141072).Curated2
Sequence conflicti635 – 636DS → EP in AAI31920 (PubMed:15489334).Curated2
Sequence conflicti643C → R in BAC31301 (PubMed:16141072).Curated1
Sequence conflicti643C → R in AAI31920 (PubMed:15489334).Curated1
Sequence conflicti657 – 659FHD → SHN in BAC31301 (PubMed:16141072).Curated3
Sequence conflicti657 – 659FHD → SHN in AAI31920 (PubMed:15489334).Curated3

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_03751574 – 149Missing in isoform 3. 2 PublicationsAdd BLAST76
Alternative sequenceiVSP_037516286 – 302Missing in isoform 2. 1 PublicationAdd BLAST17

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB162219 mRNA. Translation: BAD36742.1.
AK042589 mRNA. Translation: BAC31301.1.
BC057309 mRNA. Translation: AAH57309.1.
BC131919 mRNA. Translation: AAI31920.1.
CCDSiCCDS15144.1. [Q6PG16-1]
RefSeqiNP_766093.1. NM_172505.4.
NP_941054.2. NM_198652.2.
XP_006529783.1. XM_006529720.3.
UniGeneiMm.384762.

Genome annotation databases

GeneIDi212427.
381280.
KEGGimmu:212427.
mmu:381280.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB162219 mRNA. Translation: BAD36742.1.
AK042589 mRNA. Translation: BAC31301.1.
BC057309 mRNA. Translation: AAH57309.1.
BC131919 mRNA. Translation: AAI31920.1.
CCDSiCCDS15144.1. [Q6PG16-1]
RefSeqiNP_766093.1. NM_172505.4.
NP_941054.2. NM_198652.2.
XP_006529783.1. XM_006529720.3.
UniGeneiMm.384762.

3D structure databases

ProteinModelPortaliQ6PG16.
SMRiQ6PG16.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000054263.

PTM databases

iPTMnetiQ6PG16.
PhosphoSitePlusiQ6PG16.

Proteomic databases

PaxDbiQ6PG16.
PRIDEiQ6PG16.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi212427.
381280.
KEGGimmu:212427.
mmu:381280.

Organism-specific databases

CTDi55355.
MGIiMGI:2685821. Hjurp.

Phylogenomic databases

eggNOGiENOG410IW66. Eukaryota.
ENOG4111C4D. LUCA.
HOGENOMiHOG000112909.
InParanoidiQ6PG16.
PhylomeDBiQ6PG16.

Miscellaneous databases

PROiQ6PG16.
SOURCEiSearch...

Family and domain databases

InterProiIPR021052. HJURP.
IPR022102. HJURP_C.
IPR018465. Scm3/HJURP.
[Graphical view]
PfamiPF12347. HJURP_C. 2 hits.
PF12346. HJURP_mid. 1 hit.
PF10384. Scm3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHJURP_MOUSE
AccessioniPrimary (citable) accession number: Q6PG16
Secondary accession number(s): Q6BCZ4, Q8C9A7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 16, 2009
Last sequence update: July 5, 2004
Last modified: November 2, 2016
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.