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Protein

Holliday junction recognition protein

Gene

Hjurp

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Centromeric protein that plays a central role in the incorporation and maintenance of histone H3-like variant CENPA at centromeres. Acts as a specific chaperone for CENPA and is required for the incorporation of newly synthesized CENPA molecules into nucleosomes at replicated centromeres. Prevents CENPA-H4 tetramerization and prevents premature DNA binding by the CENPA-H4 tetramer. Directly binds Holliday junctions (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

Cell cycle

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Holliday junction recognition protein
Alternative name(s):
Fetal liver expressing gene 1 protein homolog
Short name:
mFleg1
Gene namesi
Name:Hjurp
Synonyms:Fleg1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:2685821. Hjurp.

Subcellular locationi

  • Nucleusnucleolus
  • Chromosomecentromere

  • Note: Localizes in centromeres during late telophase and early G1, when CENPA nucleosomes are assembled. Localizes to nucleolus during S phase, nucleolus site being often related to storage (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 667667Holliday junction recognition proteinPRO_0000378154Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei169 – 1691PhosphoserineBy similarity
Modified residuei185 – 1851PhosphoserineBy similarity
Modified residuei195 – 1951PhosphoserineBy similarity
Modified residuei388 – 3881PhosphoserineCombined sources
Modified residuei424 – 4241PhosphoserineBy similarity
Modified residuei449 – 4491PhosphoserineBy similarity
Modified residuei462 – 4621PhosphoserineBy similarity
Modified residuei567 – 5671PhosphoserineBy similarity
Modified residuei613 – 6131PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ6PG16.
MaxQBiQ6PG16.
PaxDbiQ6PG16.
PRIDEiQ6PG16.

PTM databases

iPTMnetiQ6PG16.
PhosphoSiteiQ6PG16.

Interactioni

Subunit structurei

Interacts with CENPA (via CATD domain); the interaction is direct and specific for CENPA since it does not interact with H3.1- or H3.3-containing nucleosomes. Heterotrimer composed of HJURP, CENPA and histone H4, where HJURP interacts with the dimer formed by CENPA and histone H4 and prevents tetramerization of CENPA and H4. Interacts with 14-3-3 family members in a phosphorylation-dependent manner. Interacts with MSH5 and NBN (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000054263.

Structurei

3D structure databases

ProteinModelPortaliQ6PG16.
SMRiQ6PG16. Positions 7-66.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

eggNOGiENOG410IW66. Eukaryota.
ENOG4111C4D. LUCA.
HOGENOMiHOG000112909.
InParanoidiQ6PG16.
PhylomeDBiQ6PG16.

Family and domain databases

InterProiIPR021052. HJURP.
IPR022102. HJURP_C.
IPR018465. Scm3/HJURP.
[Graphical view]
PfamiPF12347. HJURP_C. 2 hits.
PF12346. HJURP_mid. 1 hit.
PF10384. Scm3. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6PG16-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MESMGRQDRR LHQQLKESSS RFQTLMKRLI AKYNQPFEDD PLVEMRTLTY
60 70 80 90 100
ETPQGLRVWG GKLMKKEDKE YTQVIDRLNG QAPEGDSESS GADTSLEENW
110 120 130 140 150
PSCSSAMREA SGDPRQRQPA VPGNTLETDL RRKYLTQVDI LPQDEEYFKN
160 170 180 190 200
AEKRGGKDTV MTWVPSVTSS VTPASGCQDA ISAKSSGGPE VSALSSRGQG
210 220 230 240 250
PSYPCPADMA IVARSDGLSL LGTSSNSVSS QSFEVDDLCN VTISDLYEGM
260 270 280 290 300
MHSMSRLLRS KPSCIISTKT YINQSWKLRR RPSRKQGLHK NRTHCPRSKP
310 320 330 340 350
SQRSARKGPA SCSEPGKEAG ILRDYGNLLH VAPHKTGLEL KSVSLEGSKR
360 370 380 390 400
QVHKSSPAWK ELQMMPQKDL DLNRERENRV MTLQWLISPV KVVPRPRMLP
410 420 430 440 450
SQVEKWYREI KIKFDKLHQE YCLSSGKQPR LTDPTESWAV DVYRSGSKSP
460 470 480 490 500
GSRQDVETCR PSSPFGREKT ERPGEALEDL RGNGKSVKTK SCLLRSCPSP
510 520 530 540 550
EGSPSRSPSH SQLSSGLQEH NSEPTGKAVW PSTAISAPSI GSPGCGKDNY
560 570 580 590 600
YELKKEFNRL YQKYCLSPQR AKVTSCGRVS PMKAAAALPC QSEHLKRLNP
610 620 630 640 650
DSPQQSSQKR SISPGCHRRV LQDSTAQTAS TLVRDSWLPT KRCKLSYPVA
660
CAHQAKFHDT SGASGWP
Length:667
Mass (Da):74,199
Last modified:July 5, 2004 - v1
Checksum:i258747B28A1123C9
GO
Isoform 2 (identifier: Q6PG16-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     286-302: Missing.

Note: No experimental confirmation available.
Show »
Length:650
Mass (Da):72,243
Checksum:i2F5259C4C49B91B1
GO
Isoform 3 (identifier: Q6PG16-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     74-149: Missing.

Show »
Length:591
Mass (Da):65,763
Checksum:iC0F34D5D7552D7A3
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti12 – 121H → Q in BAC31301 (PubMed:16141072).Curated
Sequence conflicti12 – 121H → Q in AAI31920 (PubMed:15489334).Curated
Sequence conflicti16 – 161K → N in BAC31301 (PubMed:16141072).Curated
Sequence conflicti16 – 161K → N in AAI31920 (PubMed:15489334).Curated
Sequence conflicti19 – 191S → R in BAC31301 (PubMed:16141072).Curated
Sequence conflicti19 – 191S → R in AAI31920 (PubMed:15489334).Curated
Sequence conflicti46 – 461R → T in BAC31301 (PubMed:16141072).Curated
Sequence conflicti46 – 461R → T in AAI31920 (PubMed:15489334).Curated
Sequence conflicti190 – 1901E → G in BAC31301 (PubMed:16141072).Curated
Sequence conflicti190 – 1901E → G in AAI31920 (PubMed:15489334).Curated
Sequence conflicti245 – 2451D → N in BAC31301 (PubMed:16141072).Curated
Sequence conflicti245 – 2451D → N in AAI31920 (PubMed:15489334).Curated
Sequence conflicti262 – 2621P → L in BAC31301 (PubMed:16141072).Curated
Sequence conflicti262 – 2621P → L in AAI31920 (PubMed:15489334).Curated
Sequence conflicti315 – 3151P → H in BAC31301 (PubMed:16141072).Curated
Sequence conflicti315 – 3151P → H in AAI31920 (PubMed:15489334).Curated
Sequence conflicti355 – 3551S → F in BAC31301 (PubMed:16141072).Curated
Sequence conflicti355 – 3551S → F in AAI31920 (PubMed:15489334).Curated
Sequence conflicti380 – 3801V → G in BAC31301 (PubMed:16141072).Curated
Sequence conflicti380 – 3801V → G in AAI31920 (PubMed:15489334).Curated
Sequence conflicti539 – 5391S → C in BAC31301 (PubMed:16141072).Curated
Sequence conflicti539 – 5391S → C in AAI31920 (PubMed:15489334).Curated
Sequence conflicti551 – 5511Y → D in BAD36742 (Ref. 1) Curated
Sequence conflicti551 – 5511Y → D in BAC31301 (PubMed:16141072).Curated
Sequence conflicti551 – 5511Y → D in AAI31920 (PubMed:15489334).Curated
Sequence conflicti626 – 6261A → P in BAC31301 (PubMed:16141072).Curated
Sequence conflicti626 – 6261A → P in AAI31920 (PubMed:15489334).Curated
Sequence conflicti635 – 6362DS → EP in BAC31301 (PubMed:16141072).Curated
Sequence conflicti635 – 6362DS → EP in AAI31920 (PubMed:15489334).Curated
Sequence conflicti643 – 6431C → R in BAC31301 (PubMed:16141072).Curated
Sequence conflicti643 – 6431C → R in AAI31920 (PubMed:15489334).Curated
Sequence conflicti657 – 6593FHD → SHN in BAC31301 (PubMed:16141072).Curated
Sequence conflicti657 – 6593FHD → SHN in AAI31920 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei74 – 14976Missing in isoform 3. 2 PublicationsVSP_037515Add
BLAST
Alternative sequencei286 – 30217Missing in isoform 2. 1 PublicationVSP_037516Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB162219 mRNA. Translation: BAD36742.1.
AK042589 mRNA. Translation: BAC31301.1.
BC057309 mRNA. Translation: AAH57309.1.
BC131919 mRNA. Translation: AAI31920.1.
CCDSiCCDS15144.1. [Q6PG16-1]
RefSeqiNP_766093.1. NM_172505.4.
NP_941054.2. NM_198652.2.
XP_006529783.1. XM_006529720.2.
UniGeneiMm.384762.

Genome annotation databases

GeneIDi212427.
381280.
KEGGimmu:212427.
mmu:381280.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB162219 mRNA. Translation: BAD36742.1.
AK042589 mRNA. Translation: BAC31301.1.
BC057309 mRNA. Translation: AAH57309.1.
BC131919 mRNA. Translation: AAI31920.1.
CCDSiCCDS15144.1. [Q6PG16-1]
RefSeqiNP_766093.1. NM_172505.4.
NP_941054.2. NM_198652.2.
XP_006529783.1. XM_006529720.2.
UniGeneiMm.384762.

3D structure databases

ProteinModelPortaliQ6PG16.
SMRiQ6PG16. Positions 7-66.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000054263.

PTM databases

iPTMnetiQ6PG16.
PhosphoSiteiQ6PG16.

Proteomic databases

EPDiQ6PG16.
MaxQBiQ6PG16.
PaxDbiQ6PG16.
PRIDEiQ6PG16.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi212427.
381280.
KEGGimmu:212427.
mmu:381280.

Organism-specific databases

CTDi55355.
MGIiMGI:2685821. Hjurp.

Phylogenomic databases

eggNOGiENOG410IW66. Eukaryota.
ENOG4111C4D. LUCA.
HOGENOMiHOG000112909.
InParanoidiQ6PG16.
PhylomeDBiQ6PG16.

Miscellaneous databases

PROiQ6PG16.
SOURCEiSearch...

Family and domain databases

InterProiIPR021052. HJURP.
IPR022102. HJURP_C.
IPR018465. Scm3/HJURP.
[Graphical view]
PfamiPF12347. HJURP_C. 2 hits.
PF12346. HJURP_mid. 1 hit.
PF10384. Scm3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Impaired cytoplasmic localization and nuclear accumulation of a novel gene product, hFLEG1, associated with hepatocellular carcinoma development."
    Koike N., Sumii M., Ikura T., Masuda Y., Wakida K., Uchida T., Asahara T., Usui T., Shimamoto F., Chayama K., Fukumoto M., Kamiya K.
    Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
    Strain: C57BL/6J.
    Tissue: Cerebellum.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
    Strain: C57BL/6J.
    Tissue: Brain.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-388, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Spleen and Testis.

Entry informationi

Entry nameiHJURP_MOUSE
AccessioniPrimary (citable) accession number: Q6PG16
Secondary accession number(s): Q6BCZ4, Q8C9A7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 16, 2009
Last sequence update: July 5, 2004
Last modified: July 6, 2016
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.