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Protein

Tankyrase-1

Gene

Tnks

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Poly-ADP-ribosyltransferase involved in various processes such as Wnt signaling pathway, telomere length and vesicle trafficking. Acts as an activator of the Wnt signaling pathway by mediating poly-ADP-ribosylation (PARsylation) of AXIN1 and AXIN2, 2 key components of the beta-catenin destruction complex: poly-ADP-ribosylated target proteins are recognized by RNF146, which mediates their ubiquitination and subsequent degradation. Also mediates PARsylation of BLZF1 and CASC3, followed by recruitment of RNF146 and subsequent ubiquitination. Mediates PARsylation of TERF1, thereby contributing to the regulation of telomere length. Involved in centrosome maturation during prometaphase by mediating PARsylation of HEPACAM2/MIKI. May also regulate vesicle trafficking and modulate the subcellular distribution of SLC2A4/GLUT4-vesicles. May be involved in spindle pole assembly through PARsylation of NUMA1. Stimulates 26S proteasome activity (By similarity).By similarity

Catalytic activityi

NAD+ + (ADP-D-ribosyl)(n)-acceptor = nicotinamide + (ADP-D-ribosyl)(n+1)-acceptor.PROSITE-ProRule annotation

Enzyme regulationi

Specifically inhibited by XAV939, a small molecule, leading to inhibit the Wnt signaling pathway by stabilizing AXIN1 and AXIN2.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi1227ZincBy similarity1
Metal bindingi1230ZincBy similarity1
Metal bindingi1235ZincBy similarity1
Metal bindingi1238ZincBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processCell cycle, Cell division, Mitosis, mRNA transport, Protein transport, Translocation, Transport, Wnt signaling pathway
LigandMetal-binding, NAD, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-201681 TCF dependent signaling in response to WNT
R-MMU-4641257 Degradation of AXIN
R-MMU-5689880 Ub-specific processing proteases
R-MMU-8948751 Regulation of PTEN stability and activity

Names & Taxonomyi

Protein namesi
Recommended name:
Tankyrase-1 (EC:2.4.2.30)
Short name:
TANK1
Alternative name(s):
ADP-ribosyltransferase diphtheria toxin-like 5
Short name:
ARTD5
TRF1-interacting ankyrin-related ADP-ribose polymerase 1
Short name:
Tankyrase I
Gene namesi
Name:Tnks
Synonyms:Tnks1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1341087 Tnks

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Centromere, Chromosome, Cytoplasm, Cytoskeleton, Golgi apparatus, Membrane, Nuclear pore complex, Nucleus, Telomere

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3232702

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004095111 – 1320Tankyrase-1Add BLAST1320

Post-translational modificationi

Phosphorylated on serine residues by MAPK kinases upon insulin stimulation. Phosphorylated during mitosis (By similarity).By similarity
Ubiquitinated by RNF146 when auto-poly-ADP-ribosylated, leading to its degradation.By similarity
ADP-ribosylated (-auto). Poly-ADP-ribosylated protein is recognized by RNF146, followed by ubiquitination (By similarity).By similarity

Keywords - PTMi

ADP-ribosylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ6PFX9
PeptideAtlasiQ6PFX9
PRIDEiQ6PFX9

PTM databases

iPTMnetiQ6PFX9
PhosphoSitePlusiQ6PFX9

Expressioni

Gene expression databases

BgeeiENSMUSG00000031529
ExpressionAtlasiQ6PFX9 baseline and differential
GenevisibleiQ6PFX9 MM

Interactioni

Subunit structurei

Oligomerizes and associates with TNKS2. Interacts with the cytoplasmic domain of LNPEP/Otase in SLC2A4/GLUT4-vesicles. Binds to the N-terminus of telomeric TERF1 via the ANK repeats. Found in a complex with POT1; TERF1 and TINF2. Interacts with AXIN1, AXIN2, BLZF1 and CASC3. Interacts with NUMA1 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi204263, 19 interactors
DIPiDIP-61463N
IntActiQ6PFX9, 19 interactors
STRINGi10090.ENSMUSP00000033929

Chemistry databases

BindingDBiQ6PFX9

Structurei

Secondary structure

11320
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi317 – 338Combined sources22
Helixi341 – 347Combined sources7
Turni350 – 354Combined sources5
Helixi365 – 371Combined sources7
Helixi375 – 383Combined sources9
Helixi398 – 404Combined sources7
Helixi408 – 416Combined sources9
Helixi431 – 437Combined sources7
Helixi441 – 449Combined sources9
Helixi464 – 467Combined sources4
Helixi471 – 491Combined sources21
Helixi495 – 501Combined sources7
Turni502 – 504Combined sources3
Turni512 – 514Combined sources3
Helixi518 – 524Combined sources7
Helixi530 – 539Combined sources10
Helixi554 – 560Combined sources7
Helixi564 – 572Combined sources9
Helixi587 – 594Combined sources8
Helixi597 – 605Combined sources9
Helixi620 – 623Combined sources4
Helixi626 – 634Combined sources9
Beta strandi1100 – 1103Combined sources4
Helixi1109 – 1120Combined sources12
Turni1126 – 1132Combined sources7
Beta strandi1138 – 1147Combined sources10
Helixi1149 – 1165Combined sources17
Beta strandi1172 – 1177Combined sources6
Helixi1182 – 1188Combined sources7
Helixi1192 – 1194Combined sources3
Beta strandi1203 – 1209Combined sources7
Helixi1211 – 1215Combined sources5
Turni1216 – 1219Combined sources4
Helixi1221 – 1223Combined sources3
Turni1228 – 1230Combined sources3
Beta strandi1236 – 1238Combined sources3
Beta strandi1240 – 1248Combined sources9
Beta strandi1251 – 1256Combined sources6
Beta strandi1269 – 1273Combined sources5
Beta strandi1284 – 1288Combined sources5
Helixi1290 – 1292Combined sources3
Beta strandi1293 – 1303Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3UTMX-ray2.00A/B308-655[»]
4N4TX-ray2.32A/B1097-1307[»]
5HKPX-ray2.20A/B308-655[»]
ProteinModelPortaliQ6PFX9
SMRiQ6PFX9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati174 – 202ANK 1Add BLAST29
Repeati208 – 237ANK 2Add BLAST30
Repeati241 – 270ANK 3Add BLAST30
Repeati274 – 303ANK 4Add BLAST30
Repeati361 – 390ANK 5Add BLAST30
Repeati394 – 423ANK 6Add BLAST30
Repeati427 – 456ANK 7Add BLAST30
Repeati514 – 546ANK 8Add BLAST33
Repeati550 – 579ANK 9Add BLAST30
Repeati583 – 612ANK 10Add BLAST30
Repeati676 – 705ANK 11Add BLAST30
Repeati709 – 738ANK 12Add BLAST30
Repeati742 – 771ANK 13Add BLAST30
Repeati775 – 803ANK 14Add BLAST29
Repeati829 – 858ANK 15Add BLAST30
Repeati862 – 891ANK 16Add BLAST30
Repeati895 – 924ANK 17Add BLAST30
Repeati928 – 957ANK 18Add BLAST30
Domaini1019 – 1082SAMPROSITE-ProRule annotationAdd BLAST64
Domaini1105 – 1310PARP catalyticPROSITE-ProRule annotationAdd BLAST206

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi9 – 14Poly-His6
Compositional biasi20 – 83Pro-richAdd BLAST64
Compositional biasi112 – 118Poly-Gly7
Compositional biasi121 – 146Ser-richAdd BLAST26

Keywords - Domaini

ANK repeat, Repeat

Phylogenomic databases

eggNOGiKOG4177 Eukaryota
COG0666 LUCA
GeneTreeiENSGT00840000129677
HOGENOMiHOG000246964
HOVERGENiHBG059472
InParanoidiQ6PFX9
KOiK10799
OMAiDSTSCCS
OrthoDBiEOG091G00W8
PhylomeDBiQ6PFX9
TreeFamiTF326036

Family and domain databases

CDDicd00204 ANK, 5 hits
Gene3Di1.25.40.20, 5 hits
InterProiView protein in InterPro
IPR002110 Ankyrin_rpt
IPR020683 Ankyrin_rpt-contain_dom
IPR036770 Ankyrin_rpt-contain_sf
IPR012317 Poly(ADP-ribose)pol_cat_dom
IPR001660 SAM
IPR013761 SAM/pointed_sf
IPR028731 TNKS
PANTHERiPTHR24180:SF3 PTHR24180:SF3, 1 hit
PfamiView protein in Pfam
PF00023 Ank, 2 hits
PF12796 Ank_2, 4 hits
PF13606 Ank_3, 1 hit
PF00644 PARP, 1 hit
PF07647 SAM_2, 1 hit
PRINTSiPR01415 ANKYRIN
SMARTiView protein in SMART
SM00248 ANK, 17 hits
SM00454 SAM, 1 hit
SUPFAMiSSF47769 SSF47769, 1 hit
SSF48403 SSF48403, 4 hits
PROSITEiView protein in PROSITE
PS50297 ANK_REP_REGION, 1 hit
PS50088 ANK_REPEAT, 15 hits
PS51059 PARP_CATALYTIC, 1 hit
PS50105 SAM_DOMAIN, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6PFX9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAASRRSQHH HHHHQQQLQP APGASAPPPP PPPPLSPGLA PGPTPASPTA
60 70 80 90 100
GGLAPFASPR HGLALPEGDG SRDPPDRPRS PDPVDGAVCT VAAPAAVPAA
110 120 130 140 150
SAAVGVAPTP AGGGGGGGNN SASSASSPTS SSSSSPSSPG SSLAESPEAA
160 170 180 190 200
GVGSTATLGA GAAGLGPGVP AVSGALRELL EACRNGDVSR VKRLVDAANV
210 220 230 240 250
NAKDMAGRKS SPLHFAAGFG RKDVVEHLLQ MGANVHARDD GGLIPLHNAC
260 270 280 290 300
SFGHAEVVSL LLCQGADPNA RDNWNYTPLH EAAIKGKIDV CIVLLQHGAD
310 320 330 340 350
PNIRNTDGKS ALDLADPSAK AVLTGEYKKD ELLEAARSGN EEKLMALLTP
360 370 380 390 400
LNVNCHASDG RKSTPLHLAA GYNRVRIVQL LLQHGADVHA KDKGGLVPLH
410 420 430 440 450
NACSYGHYEV TELLLKHGAC VNAMDLWQFT PLHEAASKNR VEVCSLLLSH
460 470 480 490 500
GADPTLVNCH GKSAVDMAPT PELRERLTYE FKGHSLLQAA READLAKVKK
510 520 530 540 550
TLALEIINFK QPQSHETALH CAVASLHPKR KQVAELLLRK GANVNEKNKD
560 570 580 590 600
FMTPLHVAAE RAHNDVMEVL HKHGAKMNAL DSLGQTALHR AALAGHLQTC
610 620 630 640 650
RLLLSYGSDP SIISLQGFTA AQMGNEAVQQ ILSESTPMRT SDVDYRLLEA
660 670 680 690 700
SKAGDLETVK QLCSPQNVNC RDLEGRHSTP LHFAAGYNRV SVVEYLLHHG
710 720 730 740 750
ADVHAKDKGG LVPLHNACSY GHYEVAELLV RHGASVNVAD LWKFTPLHEA
760 770 780 790 800
AAKGKYEICK LLLKHGADPT KKNRDGNTPL DLVKEGDTDI QDLLRGDAAL
810 820 830 840 850
LDAAKKGCLA RVQKLCTPEN INCRDTQGRN STPLHLAAGY NNLEVAEYLL
860 870 880 890 900
EHGADVNAQD KGGLIPLHNA ASYGHVDIAA LLIKYNTCVN ATDKWAFTPL
910 920 930 940 950
HEAAQKGRTQ LCALLLAHGA DPTMKNQEGQ TPLDLATADD IRALLIDAMP
960 970 980 990 1000
PEALPTCFKP QATVVSASLI SPASTPSCLS AASSIDNLTG PLTDLAVGGA
1010 1020 1030 1040 1050
SNAGDGAAGA ERKEGEVAGL DMNISQFLKS LGLEHLRDIF ETEQITLDVL
1060 1070 1080 1090 1100
ADMGHEELKE IGINAYGHRH KLIKGVERLL GGQQGTNPYL TFHCVNQGTI
1110 1120 1130 1140 1150
LLDLAPEDKE YQSVEEEMQS TIREHRDGGN AGGIFNRYNV IRIQKVVNKK
1160 1170 1180 1190 1200
LRERFCHRQK EVSEENHNHH NERMLFHGSP FINAIIHKGF DERHAYIGGM
1210 1220 1230 1240 1250
FGAGIYFAEN SSKSNQYVYG IGGGTGCPTH KDRSCYICHR QMLFCRVTLG
1260 1270 1280 1290 1300
KSFLQFSTMK MAHAPPGHHS VIGRPSVNGL AYAEYVIYRG EQAYPEYLIT
1310 1320
YQIMKPEAPS QTATAAEQKT
Length:1,320
Mass (Da):140,944
Last modified:July 5, 2004 - v1
Checksum:iA90360DC665FFCC0
GO
Isoform 2 (identifier: Q6PFX9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-344: Missing.

Note: No experimental confirmation available.
Show »
Length:976
Mass (Da):106,651
Checksum:i8DE71F21652C3D4A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti969L → V in BAC33475 (PubMed:16141072).Curated1
Sequence conflicti1025S → I in BAC33475 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0413201 – 344Missing in isoform 2. 1 PublicationAdd BLAST344

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK048860 mRNA Translation: BAC33475.1
AC122458 Genomic DNA No translation available.
BC057370 mRNA Translation: AAH57370.1
CCDSiCCDS22242.1 [Q6PFX9-1]
RefSeqiNP_780300.2, NM_175091.3 [Q6PFX9-1]
UniGeneiMm.88364

Genome annotation databases

EnsembliENSMUST00000033929; ENSMUSP00000033929; ENSMUSG00000031529 [Q6PFX9-1]
GeneIDi21951
KEGGimmu:21951
UCSCiuc009lku.1 mouse [Q6PFX9-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiTNKS1_MOUSE
AccessioniPrimary (citable) accession number: Q6PFX9
Secondary accession number(s): Q8BX62
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 31, 2011
Last sequence update: July 5, 2004
Last modified: May 23, 2018
This is version 132 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health