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Protein

N-acetylaspartylglutamate synthase A

Gene

Rimkla

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the synthesis of N-acetyl-L-aspartyl-L-glutamate (NAAG) and N-acetyl-L-aspartyl-L-glutamyl-L-glutamate.2 Publications

Catalytic activityi

ATP + N-acetyl-L-aspartate + L-glutamate = ADP + phosphate + N-acetyl-L-aspartyl-L-glutamate.2 Publications
2 ATP + N-acetyl-L-aspartate + 2 L-glutamate = 2 ADP + 2 phosphate + N-acetyl-L-aspartyl-L-glutamyl-L-glutamate.1 Publication

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium or manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei154 – 1541ATPBy similarity
Binding sitei215 – 2151ATPBy similarity
Metal bindingi260 – 2601Magnesium or manganese 1PROSITE-ProRule annotation
Metal bindingi273 – 2731Magnesium or manganese 1PROSITE-ProRule annotation
Metal bindingi273 – 2731Magnesium or manganese 2PROSITE-ProRule annotation
Metal bindingi275 – 2751Magnesium or manganese 2PROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi189 – 19911ATPPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW
  • N-acetyl-L-aspartate-L-glutamate ligase activity Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi6.3.2.41. 3474.
6.3.2.42. 3474.

Names & Taxonomyi

Protein namesi
Recommended name:
N-acetylaspartylglutamate synthase A (EC:6.3.2.412 Publications)
Short name:
NAAG synthetase A
Short name:
NAAGS
Alternative name(s):
N-acetylaspartylglutamylglutamate synthase A (EC:6.3.2.421 Publication)
Ribosomal protein S6 modification-like protein A
Gene namesi
Name:Rimkla
Synonyms:Fam80a, Rimk
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:3040686. Rimkla.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 380380N-acetylaspartylglutamate synthase APRO_0000282569Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei319 – 3191PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ6PFX8.
PaxDbiQ6PFX8.
PRIDEiQ6PFX8.

PTM databases

iPTMnetiQ6PFX8.
PhosphoSiteiQ6PFX8.

Expressioni

Tissue specificityi

Highly expressed in spinal cord and brain.1 Publication

Gene expression databases

BgeeiENSMUSG00000048899.
GenevisibleiQ6PFX8. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000058996.

Structurei

3D structure databases

ProteinModelPortaliQ6PFX8.
SMRiQ6PFX8. Positions 68-293.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini115 – 300186ATP-graspPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the RimK family.Curated
Contains 1 ATP-grasp domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IGDC. Eukaryota.
COG0189. LUCA.
GeneTreeiENSGT00390000014577.
HOGENOMiHOG000043112.
HOVERGENiHBG108408.
InParanoidiQ6PFX8.
KOiK18311.
OMAiCHLVRHD.
OrthoDBiEOG091G09CF.
PhylomeDBiQ6PFX8.
TreeFamiTF332035.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
InterProiIPR011761. ATP-grasp.
IPR013651. ATP-grasp_RimK-type.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR004666. RpS6_RimK/Lys_biosynth_LsyX.
[Graphical view]
PfamiPF08443. RimK. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00768. rimK_fam. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6PFX8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCAQVWLLTD RLIREDYPQV QILRALRQRC SEQDVGFRAV FLDQIAVTVV
60 70 80 90 100
GGHLGLQLSQ KPLTTFPDVV VVRVSTPSVQ SDSEITILRH LEKLGCRLVN
110 120 130 140 150
RPQSILNCIN KFWTFQELAG HGVPMPDTFS YGGHEDFSKM IDEAEPLGYP
160 170 180 190 200
VVVKSTRGHR GKAVFLARDK HHLSDICHLV RHDVPYLFQK YVKESHGKDI
210 220 230 240 250
RVVVVGGQVI GSMLRCSTDG RMQSNCFLGG VGVKCPLTEQ GKQLAIQVSN
260 270 280 290 300
ILGMDFCGID LLIMDDGSFT VCEANANVGF LAFDQACNLD VGAIIADYAM
310 320 330 340 350
SLLPNRQTGK MAILPGLASP REKNEPNGCV SAQGVAESVY AITNGSTSSE
360 370 380
SEPELGEARD SSVKTMGAPP AHVAQAWLQH
Length:380
Mass (Da):41,504
Last modified:July 5, 2004 - v1
Checksum:i437D6477E6E9EE9B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti369 – 3691P → R in BAC32976 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK047164 mRNA. Translation: BAC32976.1.
AL645563 Genomic DNA. Translation: CAM14659.1.
BC057371 mRNA. Translation: AAH57371.1.
CCDSiCCDS18583.1.
RefSeqiNP_808240.2. NM_177572.4.
UniGeneiMm.206201.

Genome annotation databases

EnsembliENSMUST00000049994; ENSMUSP00000058996; ENSMUSG00000048899.
GeneIDi194237.
KEGGimmu:194237.
UCSCiuc008umo.3. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK047164 mRNA. Translation: BAC32976.1.
AL645563 Genomic DNA. Translation: CAM14659.1.
BC057371 mRNA. Translation: AAH57371.1.
CCDSiCCDS18583.1.
RefSeqiNP_808240.2. NM_177572.4.
UniGeneiMm.206201.

3D structure databases

ProteinModelPortaliQ6PFX8.
SMRiQ6PFX8. Positions 68-293.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000058996.

PTM databases

iPTMnetiQ6PFX8.
PhosphoSiteiQ6PFX8.

Proteomic databases

MaxQBiQ6PFX8.
PaxDbiQ6PFX8.
PRIDEiQ6PFX8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000049994; ENSMUSP00000058996; ENSMUSG00000048899.
GeneIDi194237.
KEGGimmu:194237.
UCSCiuc008umo.3. mouse.

Organism-specific databases

CTDi284716.
MGIiMGI:3040686. Rimkla.

Phylogenomic databases

eggNOGiENOG410IGDC. Eukaryota.
COG0189. LUCA.
GeneTreeiENSGT00390000014577.
HOGENOMiHOG000043112.
HOVERGENiHBG108408.
InParanoidiQ6PFX8.
KOiK18311.
OMAiCHLVRHD.
OrthoDBiEOG091G09CF.
PhylomeDBiQ6PFX8.
TreeFamiTF332035.

Enzyme and pathway databases

BRENDAi6.3.2.41. 3474.
6.3.2.42. 3474.

Miscellaneous databases

PROiQ6PFX8.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000048899.
GenevisibleiQ6PFX8. MM.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
InterProiIPR011761. ATP-grasp.
IPR013651. ATP-grasp_RimK-type.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR004666. RpS6_RimK/Lys_biosynth_LsyX.
[Graphical view]
PfamiPF08443. RimK. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00768. rimK_fam. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRIMKA_MOUSE
AccessioniPrimary (citable) accession number: Q6PFX8
Secondary accession number(s): Q8BXH0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: July 5, 2004
Last modified: September 7, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

N-acetyl-L-aspartyl-L-glutamate (NAAG) is the most abundant dipeptide present in vertebrate central nervous system (CNS).1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.