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Protein

Ras GTPase-activating protein 4

Gene

Rasa4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Ca2+-dependent Ras GTPase-activating protein, that switches off the Ras-MAPK pathway following a stimulus that elevates intracellular calcium. Functions as an adaptor for Cdc42 and Rac1 during FcR-mediated phagocytosis. Isoform 2 activates the Ras pathway and promotes RANKL shedding by modulating the expression of MMP14.2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri674 – 710Btk-typePROSITE-ProRule annotationAdd BLAST37

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGTPase activation
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-5658442 Regulation of RAS by GAPs

Names & Taxonomyi

Protein namesi
Recommended name:
Ras GTPase-activating protein 4
Alternative name(s):
Calcium-promoted Ras inactivator
Ras p21 protein activator 4
RasGAP-activating-like protein 2
Gene namesi
Name:Rasa4
Synonyms:Capri, Kiaa0538
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1858600 Rasa4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003236061 – 802Ras GTPase-activating protein 4Add BLAST802

Proteomic databases

PaxDbiQ6PFQ7
PeptideAtlasiQ6PFQ7
PRIDEiQ6PFQ7

PTM databases

iPTMnetiQ6PFQ7
PhosphoSitePlusiQ6PFQ7

Expressioni

Tissue specificityi

Isoform 2 is expressed in osteoblasts.1 Publication

Gene expression databases

BgeeiENSMUSG00000004952
CleanExiMM_RASA4
ExpressionAtlasiQ6PFQ7 baseline and differential
GenevisibleiQ6PFQ7 MM

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000037869

Structurei

3D structure databases

ProteinModelPortaliQ6PFQ7
SMRiQ6PFQ7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 88C2 1PROSITE-ProRule annotationAdd BLAST88
Domaini120 – 216C2 2PROSITE-ProRule annotationAdd BLAST97
Domaini301 – 511Ras-GAPPROSITE-ProRule annotationAdd BLAST211
Domaini565 – 672PHPROSITE-ProRule annotationAdd BLAST108

Domaini

The PH domain does not bind phosphatidylinositol 4,5-bisphosphate or phosphatidylinositol 3,4,5-trisphosphate. This lack of binding activity is due to Leu-591, compared to Arg found in other family members (By similarity).By similarity

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri674 – 710Btk-typePROSITE-ProRule annotationAdd BLAST37

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG2059 Eukaryota
ENOG410Y128 LUCA
GeneTreeiENSGT00760000119092
HOGENOMiHOG000234324
HOVERGENiHBG106587
InParanoidiQ6PFQ7
KOiK17630
OMAiDLGCDKT
OrthoDBiEOG091G04T8
PhylomeDBiQ6PFQ7
TreeFamiTF105302

Family and domain databases

CDDicd13372 PH_CAPRI, 1 hit
Gene3Di2.30.29.30, 1 hit
2.60.40.150, 2 hits
InterProiView protein in InterPro
IPR000008 C2_dom
IPR035892 C2_domain_sf
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
IPR037777 RASA4_PH
IPR023152 RasGAP_CS
IPR001936 RasGAP_dom
IPR008936 Rho_GTPase_activation_prot
IPR001562 Znf_Btk_motif
PfamiView protein in Pfam
PF00779 BTK, 1 hit
PF00168 C2, 2 hits
PF00169 PH, 1 hit
PF00616 RasGAP, 1 hit
PRINTSiPR00360 C2DOMAIN
SMARTiView protein in SMART
SM00107 BTK, 1 hit
SM00239 C2, 2 hits
SM00233 PH, 1 hit
SM00323 RasGAP, 1 hit
SUPFAMiSSF48350 SSF48350, 1 hit
PROSITEiView protein in PROSITE
PS50004 C2, 2 hits
PS50003 PH_DOMAIN, 1 hit
PS00509 RAS_GTPASE_ACTIV_1, 1 hit
PS50018 RAS_GTPASE_ACTIV_2, 1 hit
PS51113 ZF_BTK, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6PFQ7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAKRSSLSIR IVEGKNLPAK DITGSSDPYC IVKVDNEPII RTATVWKTLC
60 70 80 90 100
PFWGEDYQVH LPPTFHTVAF YVMDEDALSR DDVIGKVCLT RDALASHPKG
110 120 130 140 150
FSGWTHLVEV DPNEEVQGEI HLRLEVVPGV HASRLRCAVL EARDLAPKDR
160 170 180 190 200
NGASDPFVRV HYNGRTQETS VVKKSCYPRW NETFDFELEK GASEALLVEA
210 220 230 240 250
WDWDLVSRND FLGKVAVNVQ RLCSAQQEEG WFRLQPDQSK SRQGKGNLGS
260 270 280 290 300
LQLEVRLRDE TVLPSVCYQP LVQLLCQEVK LGTQGPGRLI PVIEETTSAE
310 320 330 340 350
CRQEVATTLL KLFLGQGLAK DFLDLLFQLE LGRTSEANTL FRSNSLASKS
360 370 380 390 400
MESFLKVAGM RYLHGILGPI IDRVFEEKKY VELDPSKVEV KDVGCSGLHR
410 420 430 440 450
PQTEAEVLEQ SAQTLRAHLV ALLSAICRSV RTCPAIIRAT FRQLFRRVRE
460 470 480 490 500
RFPNAQHQNV PFIAVTSFLC LRFFSPAILS PKLFHLRERH ADARTSRTLL
510 520 530 540 550
LLAKAVQNIG NMDTPVSRAK ESWMEPLQPT VRQGVAQLKD FIMKLVDIEE
560 570 580 590 600
KEELDLQRAL NSQAPPVKEG PLFIHRTKGK GPLASSSFKK LYFSLTTEAL
610 620 630 640 650
SFAKTSSSKK STFIKLASIR AAEKVEEKSF GSSHIMQVIY ADDVGRAQTV
660 670 680 690 700
YLQCKCVNEL NQWLSALRKA STNNRGLLRS YHPGIFRGDK WSCCHQKDKT
710 720 730 740 750
DQGCDKTHSR VTLQEWNDPL DHDLEAQLIY RHLLGVEAAL RERYQLLRGA
760 770 780 790 800
TEAGVSPTGC DGAPEDSLAQ LLRVLQDLRE AHGSSLASPA AREPHHLLEL

QT
Length:802
Mass (Da):90,060
Last modified:July 5, 2004 - v1
Checksum:i185EBA11B2384099
GO
Isoform 2 (identifier: Q6PFQ7-2) [UniParc]FASTAAdd to basket
Also known as: deltaCAPRI

The sequence of this isoform differs from the canonical sequence as follows:
     459-504: Missing.

Show »
Length:756
Mass (Da):84,810
Checksum:i85CC40D183044F04
GO

Sequence cautioni

The sequence BAD90143 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti208R → Q in BAD90143 (Ref. 3) Curated1
Sequence conflicti216A → V in BAD90143 (Ref. 3) Curated1
Sequence conflicti298S → I in BAE33076 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_032042459 – 504Missing in isoform 2. 1 PublicationAdd BLAST46

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY591339 mRNA Translation: AAT00515.1
DQ317932 mRNA Translation: ABC47038.1
AK220218 mRNA Translation: BAD90143.1 Different initiation.
AK154335 mRNA Translation: BAE32521.1
AK155146 mRNA Translation: BAE33076.1
BC057460 mRNA Translation: AAH57460.1
BC092143 mRNA Translation: AAH92143.1
CCDSiCCDS39324.1 [Q6PFQ7-1]
CCDS39325.1 [Q6PFQ7-2]
RefSeqiNP_001034192.1, NM_001039103.3 [Q6PFQ7-2]
NP_598675.2, NM_133914.3 [Q6PFQ7-1]
UniGeneiMm.290655

Genome annotation databases

EnsembliENSMUST00000042135; ENSMUSP00000037869; ENSMUSG00000004952 [Q6PFQ7-1]
ENSMUST00000100570; ENSMUSP00000098136; ENSMUSG00000004952 [Q6PFQ7-2]
GeneIDi54153
KEGGimmu:54153
UCSCiuc008zzt.2 mouse [Q6PFQ7-1]
uc008zzu.2 mouse [Q6PFQ7-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiRASL2_MOUSE
AccessioniPrimary (citable) accession number: Q6PFQ7
Secondary accession number(s): Q2PMI6
, Q3U2R4, Q571H1, Q58DY7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: July 5, 2004
Last modified: March 28, 2018
This is version 128 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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