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Protein

Ras GTPase-activating protein 4

Gene

Rasa4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Ca2+-dependent Ras GTPase-activating protein, that switches off the Ras-MAPK pathway following a stimulus that elevates intracellular calcium. Functions as an adaptor for Cdc42 and Rac1 during FcR-mediated phagocytosis. Isoform 2 activates the Ras pathway and promotes RANKL shedding by modulating the expression of MMP14.2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri674 – 71037Btk-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-5658442. Regulation of RAS by GAPs.
R-MMU-8849471. PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases.

Names & Taxonomyi

Protein namesi
Recommended name:
Ras GTPase-activating protein 4
Alternative name(s):
Calcium-promoted Ras inactivator
Ras p21 protein activator 4
RasGAP-activating-like protein 2
Gene namesi
Name:Rasa4
Synonyms:Capri, Kiaa0538
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1858600. Rasa4.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 802802Ras GTPase-activating protein 4PRO_0000323606Add
BLAST

Proteomic databases

MaxQBiQ6PFQ7.
PaxDbiQ6PFQ7.
PeptideAtlasiQ6PFQ7.
PRIDEiQ6PFQ7.

PTM databases

iPTMnetiQ6PFQ7.
PhosphoSiteiQ6PFQ7.

Expressioni

Tissue specificityi

Isoform 2 is expressed in osteoblasts.1 Publication

Gene expression databases

BgeeiENSMUSG00000004952.
CleanExiMM_RASA4.
ExpressionAtlasiQ6PFQ7. baseline and differential.
GenevisibleiQ6PFQ7. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000037869.

Structurei

3D structure databases

ProteinModelPortaliQ6PFQ7.
SMRiQ6PFQ7. Positions 7-560, 572-706.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 8888C2 1PROSITE-ProRule annotationAdd
BLAST
Domaini120 – 21697C2 2PROSITE-ProRule annotationAdd
BLAST
Domaini301 – 511211Ras-GAPPROSITE-ProRule annotationAdd
BLAST
Domaini565 – 672108PHPROSITE-ProRule annotationAdd
BLAST

Domaini

The PH domain does not bind phosphatidylinositol 4,5-bisphosphate or phosphatidylinositol 3,4,5-trisphosphate. This lack of binding activity is due to Leu-591, compared to Arg found in other family members (By similarity).By similarity

Sequence similaritiesi

Contains 1 Btk-type zinc finger.PROSITE-ProRule annotation
Contains 2 C2 domains.PROSITE-ProRule annotation
Contains 1 PH domain.PROSITE-ProRule annotation
Contains 1 Ras-GAP domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri674 – 71037Btk-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG2059. Eukaryota.
ENOG410Y128. LUCA.
GeneTreeiENSGT00760000119092.
HOGENOMiHOG000234324.
HOVERGENiHBG106587.
InParanoidiQ6PFQ7.
KOiK17630.
OMAiDLGCDKT.
OrthoDBiEOG091G04T8.
PhylomeDBiQ6PFQ7.
TreeFamiTF105302.

Family and domain databases

Gene3Di1.10.506.10. 1 hit.
2.30.29.30. 1 hit.
2.60.40.150. 2 hits.
InterProiIPR000008. C2_dom.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR023152. RasGAP_CS.
IPR001936. RasGAP_dom.
IPR008936. Rho_GTPase_activation_prot.
IPR001562. Znf_Btk_motif.
[Graphical view]
PfamiPF00779. BTK. 1 hit.
PF00168. C2. 2 hits.
PF00169. PH. 1 hit.
PF00616. RasGAP. 1 hit.
[Graphical view]
PRINTSiPR00360. C2DOMAIN.
SMARTiSM00107. BTK. 1 hit.
SM00239. C2. 2 hits.
SM00233. PH. 1 hit.
SM00323. RasGAP. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
SSF49562. SSF49562. 2 hits.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50004. C2. 2 hits.
PS50003. PH_DOMAIN. 1 hit.
PS00509. RAS_GTPASE_ACTIV_1. 1 hit.
PS50018. RAS_GTPASE_ACTIV_2. 1 hit.
PS51113. ZF_BTK. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6PFQ7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAKRSSLSIR IVEGKNLPAK DITGSSDPYC IVKVDNEPII RTATVWKTLC
60 70 80 90 100
PFWGEDYQVH LPPTFHTVAF YVMDEDALSR DDVIGKVCLT RDALASHPKG
110 120 130 140 150
FSGWTHLVEV DPNEEVQGEI HLRLEVVPGV HASRLRCAVL EARDLAPKDR
160 170 180 190 200
NGASDPFVRV HYNGRTQETS VVKKSCYPRW NETFDFELEK GASEALLVEA
210 220 230 240 250
WDWDLVSRND FLGKVAVNVQ RLCSAQQEEG WFRLQPDQSK SRQGKGNLGS
260 270 280 290 300
LQLEVRLRDE TVLPSVCYQP LVQLLCQEVK LGTQGPGRLI PVIEETTSAE
310 320 330 340 350
CRQEVATTLL KLFLGQGLAK DFLDLLFQLE LGRTSEANTL FRSNSLASKS
360 370 380 390 400
MESFLKVAGM RYLHGILGPI IDRVFEEKKY VELDPSKVEV KDVGCSGLHR
410 420 430 440 450
PQTEAEVLEQ SAQTLRAHLV ALLSAICRSV RTCPAIIRAT FRQLFRRVRE
460 470 480 490 500
RFPNAQHQNV PFIAVTSFLC LRFFSPAILS PKLFHLRERH ADARTSRTLL
510 520 530 540 550
LLAKAVQNIG NMDTPVSRAK ESWMEPLQPT VRQGVAQLKD FIMKLVDIEE
560 570 580 590 600
KEELDLQRAL NSQAPPVKEG PLFIHRTKGK GPLASSSFKK LYFSLTTEAL
610 620 630 640 650
SFAKTSSSKK STFIKLASIR AAEKVEEKSF GSSHIMQVIY ADDVGRAQTV
660 670 680 690 700
YLQCKCVNEL NQWLSALRKA STNNRGLLRS YHPGIFRGDK WSCCHQKDKT
710 720 730 740 750
DQGCDKTHSR VTLQEWNDPL DHDLEAQLIY RHLLGVEAAL RERYQLLRGA
760 770 780 790 800
TEAGVSPTGC DGAPEDSLAQ LLRVLQDLRE AHGSSLASPA AREPHHLLEL

QT
Length:802
Mass (Da):90,060
Last modified:July 5, 2004 - v1
Checksum:i185EBA11B2384099
GO
Isoform 2 (identifier: Q6PFQ7-2) [UniParc]FASTAAdd to basket
Also known as: deltaCAPRI

The sequence of this isoform differs from the canonical sequence as follows:
     459-504: Missing.

Show »
Length:756
Mass (Da):84,810
Checksum:i85CC40D183044F04
GO

Sequence cautioni

The sequence BAD90143 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti208 – 2081R → Q in BAD90143 (Ref. 3) Curated
Sequence conflicti216 – 2161A → V in BAD90143 (Ref. 3) Curated
Sequence conflicti298 – 2981S → I in BAE33076 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei459 – 50446Missing in isoform 2. 1 PublicationVSP_032042Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY591339 mRNA. Translation: AAT00515.1.
DQ317932 mRNA. Translation: ABC47038.1.
AK220218 mRNA. Translation: BAD90143.1. Different initiation.
AK154335 mRNA. Translation: BAE32521.1.
AK155146 mRNA. Translation: BAE33076.1.
BC057460 mRNA. Translation: AAH57460.1.
BC092143 mRNA. Translation: AAH92143.1.
CCDSiCCDS39324.1. [Q6PFQ7-1]
CCDS39325.1. [Q6PFQ7-2]
RefSeqiNP_001034192.1. NM_001039103.3. [Q6PFQ7-2]
NP_598675.2. NM_133914.3. [Q6PFQ7-1]
UniGeneiMm.290655.

Genome annotation databases

EnsembliENSMUST00000042135; ENSMUSP00000037869; ENSMUSG00000004952. [Q6PFQ7-1]
ENSMUST00000100570; ENSMUSP00000098136; ENSMUSG00000004952. [Q6PFQ7-2]
GeneIDi54153.
KEGGimmu:54153.
UCSCiuc008zzt.2. mouse. [Q6PFQ7-1]
uc008zzu.2. mouse. [Q6PFQ7-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY591339 mRNA. Translation: AAT00515.1.
DQ317932 mRNA. Translation: ABC47038.1.
AK220218 mRNA. Translation: BAD90143.1. Different initiation.
AK154335 mRNA. Translation: BAE32521.1.
AK155146 mRNA. Translation: BAE33076.1.
BC057460 mRNA. Translation: AAH57460.1.
BC092143 mRNA. Translation: AAH92143.1.
CCDSiCCDS39324.1. [Q6PFQ7-1]
CCDS39325.1. [Q6PFQ7-2]
RefSeqiNP_001034192.1. NM_001039103.3. [Q6PFQ7-2]
NP_598675.2. NM_133914.3. [Q6PFQ7-1]
UniGeneiMm.290655.

3D structure databases

ProteinModelPortaliQ6PFQ7.
SMRiQ6PFQ7. Positions 7-560, 572-706.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000037869.

PTM databases

iPTMnetiQ6PFQ7.
PhosphoSiteiQ6PFQ7.

Proteomic databases

MaxQBiQ6PFQ7.
PaxDbiQ6PFQ7.
PeptideAtlasiQ6PFQ7.
PRIDEiQ6PFQ7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000042135; ENSMUSP00000037869; ENSMUSG00000004952. [Q6PFQ7-1]
ENSMUST00000100570; ENSMUSP00000098136; ENSMUSG00000004952. [Q6PFQ7-2]
GeneIDi54153.
KEGGimmu:54153.
UCSCiuc008zzt.2. mouse. [Q6PFQ7-1]
uc008zzu.2. mouse. [Q6PFQ7-2]

Organism-specific databases

CTDi10156.
MGIiMGI:1858600. Rasa4.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG2059. Eukaryota.
ENOG410Y128. LUCA.
GeneTreeiENSGT00760000119092.
HOGENOMiHOG000234324.
HOVERGENiHBG106587.
InParanoidiQ6PFQ7.
KOiK17630.
OMAiDLGCDKT.
OrthoDBiEOG091G04T8.
PhylomeDBiQ6PFQ7.
TreeFamiTF105302.

Enzyme and pathway databases

ReactomeiR-MMU-5658442. Regulation of RAS by GAPs.
R-MMU-8849471. PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases.

Miscellaneous databases

PROiQ6PFQ7.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000004952.
CleanExiMM_RASA4.
ExpressionAtlasiQ6PFQ7. baseline and differential.
GenevisibleiQ6PFQ7. MM.

Family and domain databases

Gene3Di1.10.506.10. 1 hit.
2.30.29.30. 1 hit.
2.60.40.150. 2 hits.
InterProiIPR000008. C2_dom.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR023152. RasGAP_CS.
IPR001936. RasGAP_dom.
IPR008936. Rho_GTPase_activation_prot.
IPR001562. Znf_Btk_motif.
[Graphical view]
PfamiPF00779. BTK. 1 hit.
PF00168. C2. 2 hits.
PF00169. PH. 1 hit.
PF00616. RasGAP. 1 hit.
[Graphical view]
PRINTSiPR00360. C2DOMAIN.
SMARTiSM00107. BTK. 1 hit.
SM00239. C2. 2 hits.
SM00233. PH. 1 hit.
SM00323. RasGAP. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
SSF49562. SSF49562. 2 hits.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50004. C2. 2 hits.
PS50003. PH_DOMAIN. 1 hit.
PS00509. RAS_GTPASE_ACTIV_1. 1 hit.
PS50018. RAS_GTPASE_ACTIV_2. 1 hit.
PS51113. ZF_BTK. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRASL2_MOUSE
AccessioniPrimary (citable) accession number: Q6PFQ7
Secondary accession number(s): Q2PMI6
, Q3U2R4, Q571H1, Q58DY7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: July 5, 2004
Last modified: September 7, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.