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Protein

E3 ubiquitin-protein ligase arih1

Gene

arih1

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

E3 ubiquitin-protein ligase, which catalyzes ubiquitination of target proteins together with ubiquitin-conjugating enzyme E2 ube2l3. Acts as an atypical E3 ubiquitin-protein ligase by working together with cullin-RING ubiquitin ligase (CRL) complexes and initiating ubiquitination of CRL substrates: associates with CRL complexes and specifically mediates addition of the first ubiquitin on CRLs targets. The initial ubiquitin is then elongated. E3 ubiquitin-protein ligase activity is activated upon binding to neddylated cullin-RING ubiquitin ligase complexes.By similarity

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.By similarity

Enzyme regulationi

Autoinhibited by the ariadne domain, which masks the second RING-type zinc finger that contains the active site and inhibits the E3 activity. Inhibition is relieved upon binding to neddylated cullin-RING ubiquitin ligase complexes, which activate the E3 ligase activity of ARIH1.By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi156Zinc 1By similarity1
Metal bindingi159Zinc 1By similarity1
Metal bindingi173Zinc 2By similarity1
Metal bindingi175Zinc 2; via pros nitrogenBy similarity1
Metal bindingi178Zinc 1By similarity1
Metal bindingi181Zinc 1By similarity1
Metal bindingi201Zinc 2By similarity1
Metal bindingi206Zinc 2By similarity1
Metal bindingi246Zinc 3By similarity1
Metal bindingi251Zinc 3By similarity1
Metal bindingi267Zinc 3By similarity1
Metal bindingi269Zinc 3By similarity1
Metal bindingi274Zinc 4By similarity1
Metal bindingi277Zinc 4By similarity1
Metal bindingi282Zinc 4; via tele nitrogenBy similarity1
Metal bindingi287Zinc 4By similarity1
Metal bindingi314Zinc 5By similarity1
Metal bindingi317Zinc 5By similarity1
Active sitei327By similarity1
Metal bindingi332Zinc 5By similarity1
Metal bindingi337Zinc 5By similarity1
Metal bindingi342Zinc 6By similarity1
Metal bindingi345Zinc 6By similarity1
Metal bindingi352Zinc 6; via tele nitrogenBy similarity1
Metal bindingi359Zinc 6By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri156 – 206RING-type 1; atypicalPROSITE-ProRule annotationAdd BLAST51
Zinc fingeri226 – 287IBR-typeAdd BLAST62
Zinc fingeri314 – 345RING-type 2PROSITE-ProRule annotationAdd BLAST32

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-DRE-1169408. ISG15 antiviral mechanism.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase arih1 (EC:2.3.2.-By similarity)
Alternative name(s):
Protein ariadne-1 homolog
Short name:
ARI-1
RING-type E3 ubiquitin transferase arih1Curated
Gene namesi
Name:arih1
ORF Names:si:dkey-151p17.4, si:dkey-73n10.2
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Chromosome 18

Organism-specific databases

ZFINiZDB-GENE-030131-5213. arih1.

Subcellular locationi

  • Cytoplasm By similarity
  • Nucleus By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004108951 – 527E3 ubiquitin-protein ligase arih1Add BLAST527

Proteomic databases

PaxDbiQ6PFJ9.
PRIDEiQ6PFJ9.

Expressioni

Gene expression databases

BgeeiENSDARG00000003616.
ExpressionAtlasiQ6PFJ9. differential.

Interactioni

Subunit structurei

Interacts (via the first RING-type zinc finger) with ube2l3. Associates with cullin-RING ubiquitin ligase (CRL) complexes containing neddylated cullin.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi7955.ENSDARP00000006476.

Structurei

3D structure databases

ProteinModelPortaliQ6PFJ9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni75 – 123UBA-likeBy similarityAdd BLAST49
Regioni378 – 527Ariadne domainBy similarityAdd BLAST150

Domaini

Members of the RBR family are atypical E3 ligases. They interact with the E2 conjugating enzyme UBE2L3 and function like HECT-type E3 enzymes: they bind E2s via the first RING-type zinc finger, but require an obligate trans-thiolation step during the ubiquitin transfer, requiring a conserved active site Cys residue in the second RING-type zinc finger. The active site probably forms a thioester intermediate with ubiquitin taken from the active-site cysteine of the E2 before ultimately transferring it to a Lys residue on the substrate.By similarity
The Ariadne domain inhibits activity by masking the second RING-type zinc finger that contains the active site.By similarity

Sequence similaritiesi

Belongs to the RBR family. Ariadne subfamily.Curated
Contains 1 IBR-type zinc finger.Curated
Contains 2 RING-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri156 – 206RING-type 1; atypicalPROSITE-ProRule annotationAdd BLAST51
Zinc fingeri226 – 287IBR-typeAdd BLAST62
Zinc fingeri314 – 345RING-type 2PROSITE-ProRule annotationAdd BLAST32

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1815. Eukaryota.
ENOG410XP9Y. LUCA.
GeneTreeiENSGT00840000129790.
HOGENOMiHOG000216612.
HOVERGENiHBG018737.
InParanoidiQ6PFJ9.
KOiK11968.
PhylomeDBiQ6PFJ9.
TreeFamiTF300805.

Family and domain databases

Gene3Di3.30.40.10. 2 hits.
InterProiIPR031127. E3_UB_ligase_RBR.
IPR002867. IBR_dom.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PANTHERiPTHR11685. PTHR11685. 1 hit.
PfamiPF01485. IBR. 2 hits.
[Graphical view]
SMARTiSM00647. IBR. 2 hits.
[Graphical view]
PROSITEiPS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6PFJ9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDSDEGYNYE FDDEEEECSE DSGEEETADD TLELGEVELV DPVVAGGERD
60 70 80 90 100
DCGETGGSGL GPGQDEEDYR FEVLTAEQIL QHMVECIREV NEVIQNPATI
110 120 130 140 150
TRILLSHFNW DKEKLMERYF DGNLDKLFSE CHVINPSKKS RTRLMNTRSS
160 170 180 190 200
AQDMPCQICY LNYPNSYFTG LECGHKFCMQ CWGDYLTTKI IEEGMGQTIS
210 220 230 240 250
CPAHSCDILV DDNTVMRLIT DSKVKLKYQH LITNSFVECN RLLKWCPAPD
260 270 280 290 300
CHHVVKVQYP DAKPVRCKCG RQFCFNCGEN WHDPVKCKWL RKWIKKCDDD
310 320 330 340 350
SETSNWIAAN TKECPKCHVT IEKDGGCNHM VCRNQNCKAE FCWVCLGPWE
360 370 380 390 400
PHGSAWYNCN RYNEDDAKAA RDAQERSRAA LQRYLFYCNR YMNHMQSLRF
410 420 430 440 450
EHKLYAQVKQ KMEEMQQHNM SWIEVQFLKK AVDVLCQCRS TLMFTYVFAF
460 470 480 490 500
YLKKNNQSII FENNQADLEN ATEVLSGYLE RDISQDSLQD IKQKVQDKYR
510 520
YCESRRRVLL QHVHEGYDKD LWEYIED
Length:527
Mass (Da):61,654
Last modified:July 5, 2004 - v1
Checksum:iF3997B436DECD003
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX571681 Genomic DNA. Translation: CAM56728.1.
BX571883 Genomic DNA. Translation: CAQ13584.1.
BC057523 mRNA. Translation: AAH57523.1.
RefSeqiNP_956052.1. NM_199758.1.
UniGeneiDr.75869.

Genome annotation databases

EnsembliENSDART00000019581; ENSDARP00000006476; ENSDARG00000003616.
GeneIDi327005.
KEGGidre:327005.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX571681 Genomic DNA. Translation: CAM56728.1.
BX571883 Genomic DNA. Translation: CAQ13584.1.
BC057523 mRNA. Translation: AAH57523.1.
RefSeqiNP_956052.1. NM_199758.1.
UniGeneiDr.75869.

3D structure databases

ProteinModelPortaliQ6PFJ9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7955.ENSDARP00000006476.

Proteomic databases

PaxDbiQ6PFJ9.
PRIDEiQ6PFJ9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSDART00000019581; ENSDARP00000006476; ENSDARG00000003616.
GeneIDi327005.
KEGGidre:327005.

Organism-specific databases

CTDi25820.
ZFINiZDB-GENE-030131-5213. arih1.

Phylogenomic databases

eggNOGiKOG1815. Eukaryota.
ENOG410XP9Y. LUCA.
GeneTreeiENSGT00840000129790.
HOGENOMiHOG000216612.
HOVERGENiHBG018737.
InParanoidiQ6PFJ9.
KOiK11968.
PhylomeDBiQ6PFJ9.
TreeFamiTF300805.

Enzyme and pathway databases

UniPathwayiUPA00143.
ReactomeiR-DRE-1169408. ISG15 antiviral mechanism.

Miscellaneous databases

PROiQ6PFJ9.

Gene expression databases

BgeeiENSDARG00000003616.
ExpressionAtlasiQ6PFJ9. differential.

Family and domain databases

Gene3Di3.30.40.10. 2 hits.
InterProiIPR031127. E3_UB_ligase_RBR.
IPR002867. IBR_dom.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PANTHERiPTHR11685. PTHR11685. 1 hit.
PfamiPF01485. IBR. 2 hits.
[Graphical view]
SMARTiSM00647. IBR. 2 hits.
[Graphical view]
PROSITEiPS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiARI1_DANRE
AccessioniPrimary (citable) accession number: Q6PFJ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 28, 2011
Last sequence update: July 5, 2004
Last modified: November 30, 2016
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.