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Protein

E3 ubiquitin-protein ligase arih1

Gene

arih1

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

E3 ubiquitin-protein ligase, which catalyzes polyubiquitination of target proteins together with ubiquitin-conjugating enzyme E2 ube2l3.By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi314 – 3141ZincBy similarity
Metal bindingi317 – 3171ZincBy similarity
Metal bindingi332 – 3321ZincBy similarity
Metal bindingi337 – 3371ZincBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri156 – 20651RING-type 1; atypicalPROSITE-ProRule annotationAdd
BLAST
Zinc fingeri226 – 28762IBR-typeAdd
BLAST
Zinc fingeri314 – 34532RING-type 2PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-DRE-1169408. ISG15 antiviral mechanism.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase arih1 (EC:6.3.2.-)
Alternative name(s):
Protein ariadne-1 homolog
Short name:
ARI-1
Gene namesi
Name:arih1
ORF Names:si:dkey-151p17.4, si:dkey-73n10.2
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Chromosome 18

Organism-specific databases

ZFINiZDB-GENE-030131-5213. arih1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 527527E3 ubiquitin-protein ligase arih1PRO_0000410895Add
BLAST

Proteomic databases

PaxDbiQ6PFJ9.

Expressioni

Gene expression databases

BgeeiQ6PFJ9.

Interactioni

Protein-protein interaction databases

STRINGi7955.ENSDARP00000006476.

Structurei

3D structure databases

ProteinModelPortaliQ6PFJ9.
SMRiQ6PFJ9. Positions 306-364.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili403 – 41917Sequence analysisAdd
BLAST

Domaini

The RING-type 2 zinc finger is atypical: it only binds 1 zinc ion instead of 2 and uses a different hydrophobic network compared to classical RING-types.By similarity

Sequence similaritiesi

Belongs to the RBR family. Ariadne subfamily.Curated
Contains 1 IBR-type zinc finger.Curated
Contains 2 RING-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri156 – 20651RING-type 1; atypicalPROSITE-ProRule annotationAdd
BLAST
Zinc fingeri226 – 28762IBR-typeAdd
BLAST
Zinc fingeri314 – 34532RING-type 2PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Coiled coil, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1815. Eukaryota.
ENOG410XP9Y. LUCA.
GeneTreeiENSGT00840000129790.
HOGENOMiHOG000216612.
HOVERGENiHBG018737.
InParanoidiQ6PFJ9.
KOiK11968.
OrthoDBiEOG7NKKJX.
PhylomeDBiQ6PFJ9.
TreeFamiTF300805.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR031127. E3_UB_ligase_RBR.
IPR002867. IBR_dom.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PANTHERiPTHR11685. PTHR11685. 1 hit.
PfamiPF01485. IBR. 2 hits.
[Graphical view]
SMARTiSM00647. IBR. 2 hits.
[Graphical view]
PROSITEiPS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6PFJ9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDSDEGYNYE FDDEEEECSE DSGEEETADD TLELGEVELV DPVVAGGERD
60 70 80 90 100
DCGETGGSGL GPGQDEEDYR FEVLTAEQIL QHMVECIREV NEVIQNPATI
110 120 130 140 150
TRILLSHFNW DKEKLMERYF DGNLDKLFSE CHVINPSKKS RTRLMNTRSS
160 170 180 190 200
AQDMPCQICY LNYPNSYFTG LECGHKFCMQ CWGDYLTTKI IEEGMGQTIS
210 220 230 240 250
CPAHSCDILV DDNTVMRLIT DSKVKLKYQH LITNSFVECN RLLKWCPAPD
260 270 280 290 300
CHHVVKVQYP DAKPVRCKCG RQFCFNCGEN WHDPVKCKWL RKWIKKCDDD
310 320 330 340 350
SETSNWIAAN TKECPKCHVT IEKDGGCNHM VCRNQNCKAE FCWVCLGPWE
360 370 380 390 400
PHGSAWYNCN RYNEDDAKAA RDAQERSRAA LQRYLFYCNR YMNHMQSLRF
410 420 430 440 450
EHKLYAQVKQ KMEEMQQHNM SWIEVQFLKK AVDVLCQCRS TLMFTYVFAF
460 470 480 490 500
YLKKNNQSII FENNQADLEN ATEVLSGYLE RDISQDSLQD IKQKVQDKYR
510 520
YCESRRRVLL QHVHEGYDKD LWEYIED
Length:527
Mass (Da):61,654
Last modified:July 5, 2004 - v1
Checksum:iF3997B436DECD003
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX571681 Genomic DNA. Translation: CAM56728.1.
BX571883 Genomic DNA. Translation: CAQ13584.1.
BC057523 mRNA. Translation: AAH57523.1.
RefSeqiNP_956052.1. NM_199758.1.
UniGeneiDr.75869.

Genome annotation databases

EnsembliENSDART00000019581; ENSDARP00000006476; ENSDARG00000003616.
GeneIDi327005.
KEGGidre:327005.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX571681 Genomic DNA. Translation: CAM56728.1.
BX571883 Genomic DNA. Translation: CAQ13584.1.
BC057523 mRNA. Translation: AAH57523.1.
RefSeqiNP_956052.1. NM_199758.1.
UniGeneiDr.75869.

3D structure databases

ProteinModelPortaliQ6PFJ9.
SMRiQ6PFJ9. Positions 306-364.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7955.ENSDARP00000006476.

Proteomic databases

PaxDbiQ6PFJ9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSDART00000019581; ENSDARP00000006476; ENSDARG00000003616.
GeneIDi327005.
KEGGidre:327005.

Organism-specific databases

CTDi25820.
ZFINiZDB-GENE-030131-5213. arih1.

Phylogenomic databases

eggNOGiKOG1815. Eukaryota.
ENOG410XP9Y. LUCA.
GeneTreeiENSGT00840000129790.
HOGENOMiHOG000216612.
HOVERGENiHBG018737.
InParanoidiQ6PFJ9.
KOiK11968.
OrthoDBiEOG7NKKJX.
PhylomeDBiQ6PFJ9.
TreeFamiTF300805.

Enzyme and pathway databases

UniPathwayiUPA00143.
ReactomeiR-DRE-1169408. ISG15 antiviral mechanism.

Miscellaneous databases

PROiQ6PFJ9.

Gene expression databases

BgeeiQ6PFJ9.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR031127. E3_UB_ligase_RBR.
IPR002867. IBR_dom.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PANTHERiPTHR11685. PTHR11685. 1 hit.
PfamiPF01485. IBR. 2 hits.
[Graphical view]
SMARTiSM00647. IBR. 2 hits.
[Graphical view]
PROSITEiPS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The zebrafish reference genome sequence and its relationship to the human genome."
    Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M., Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I., Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.
    , White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y., Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B., Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S., Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M., Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J., Clee C., Oliver K., Clark R., Riddle C., Eliott D., Threadgold G., Harden G., Ware D., Mortimer B., Kerry G., Heath P., Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S., Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J., Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J., Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D., McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S., Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E., Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A., Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P., Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J., Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E., Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C., Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C., Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M., Oberlander M., Rudolph-Geiger S., Teucke M., Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M., Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M., de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C., Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.
    Nature 496:498-503(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Tuebingen.
  2. NIH - Zebrafish Gene Collection (ZGC) project
    Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Entry informationi

Entry nameiARI1_DANRE
AccessioniPrimary (citable) accession number: Q6PFJ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 28, 2011
Last sequence update: July 5, 2004
Last modified: June 8, 2016
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Members of the RBR family are atypical E3 ligases. They interact with the E2 conjugating enzyme ube2l3 and function like HECT-type E3 enzymes: they bind E2s via the first RING domain, but require an obligate trans-thiolation step during the ubiquitin transfer, requiring a conserved cysteine residue in the second RING domain (By similarity).By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.