Q6PFJ7 (NSMA3_DANRE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sphingomyelin phosphodiesterase 4
EC=3.1.4.12

Alternative name(s):
Neutral sphingomyelinase 3
Short name=nSMase-3
Short name=nSMase3
Neutral sphingomyelinase III

Gene names

Name:	smpd4
ORF Names:	zgc:66367

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	791 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the hydrolysis of membrane sphingomyelin to form phosphorylcholine and ceramide By similarity.
Catalytic activity	Sphingomyelin + H2O = N-acylsphingosine + choline phosphate.
Cofactor	Magnesium By similarity.
Subcellular location	Endoplasmic reticulum membrane; Single-pass membrane protein. Golgi apparatus membrane; Single-pass membrane protein By similarity.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Golgi apparatus Membrane
Domain	Transmembrane Transmembrane helix
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW sphingomyelin phosphodiesterase D activity Inferred from electronic annotation. Source: InterPro sphingomyelin phosphodiesterase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 791	791	Sphingomyelin phosphodiesterase 4		PRO_0000273165
Regions
	Transmembrane	755 – 775	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	Q6PFJ7 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 6EFCAD6148CF7D78	FASTA	791	89,990
	10 20 30 40 50 60 MAASALQQPS YLLANLKADW TNKPLHQRCH ELCKIIDDYP AKELHAIFPW LVECVFGSLD 70 80 90 100 110 120 GILTGWNLRF LQARSAEYSI AMEFLDPSGP MMKLVYKLQA EEYKYEFPIS YLPGPIKSSI 130 140 150 160 170 180 HAGVLPDCPL FHNKIQFPMS GLLFLNPFEY YMFNFASSLI APKNYPQGQH GSSSDSAYFV 190 200 210 220 230 240 LVDTYLKYFL PTEGNVPPSP FSDTRGTVAS PAPRSTNVPY VGYGGHSTSL LKRHITHQSS 250 260 270 280 290 300 VNADPAAQEI WRSETLLQVF VEMWLHHYSL EMYQKLQSPQ VKEPFMPSEE HVLVVRLLVK 310 320 330 340 350 360 HLHTFSSSLK PESISSSPSA HSHSSPLEEL KRVVVQRFVQ QKLYVFLQHC FGHWPLDASF 370 380 390 400 410 420 RAVLETWLSY IQPWRYTGDK NNTQTDGPNR TVPDKWASFV QENLLLYTKL FQGFLNRAMR 430 440 450 460 470 480 TDLVNAKNAL MVFRVAKVFA QPSLSEMIQK GEQLFLEPEH AILQRHNRVF LTPSHGGSFL 490 500 510 520 530 540 SARQPMGTDN VFKVKSHVYS LEGQDCQYNL MFGPDQRKNV LKLIQIIAQA RQTAKRISDH 550 560 570 580 590 600 STEMAANNSF LSWFGVGSPD HNSTFTGGEM DEMGGEGVKK THEFLDKALD YLCQIFRLNA 610 620 630 640 650 660 GQLSQLISNV ASVDNNGASK QLPDCIPSEN GLVLTDLGRL QIINGLRRFE IEYQGDPELQ 670 680 690 700 710 720 PIRSYENAFL VRLLFQISSF INERLGEHME VLCSRQDFLG SVGRHYLSSS SAVVEQRRKS 730 740 750 760 770 780 PVTRQMRDRP QRARLSLRAL ASYRTLLTLL LLYMLFALLS FGLFSSTGLI LIISFLYELL 790 SNFFHEKLKT H

References

[1]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: SJD.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC057525 mRNA. Translation: AAH57525.1.
IPI	IPI00494494.
RefSeq	NP_998520.1. NM_213355.1.
UniGene	Dr.76789.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q6PFJ7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	793294.
KEGG	dre:793294.
Organism-specific databases
CTD	55627.
ZFIN	ZDB-GENE-040426-2672. smpd4.
Phylogenomic databases
eggNOG	fiNOG13032.
GeneTree	ENSGT00390000006044.
HOGENOM	HBG357187.
HOVERGEN	HBG082060.
InParanoid	Q6PFJ7.
OrthoDB	EOG4S7JPM.
Gene expression databases
Bgee	Q6PFJ7.
Family and domain databases
InterPro	IPR024129. Sphingomy_SMPD4. [Graphical view]
KO	K12353.
PANTHER	PTHR12988. PTHR12988. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NSMA3_DANRE

Accession

Primary (citable) accession number: Q6PFJ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2007
Last sequence update:	July 5, 2004
Last modified:	November 16, 2011
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program