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Q6PFE3 (RA54B_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA repair and recombination protein RAD54B

EC=3.6.4.-
Alternative name(s):
RAD54 homolog B
Gene names
Name:Rad54b
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length886 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Involved in DNA repair and mitotic recombination. May play an active role in recombination processes in concert with other members of the RAD52 epistasis group. Ref.3

Subunit structure

Interacts with RAD51 through the NH2-terminal domain By similarity.

Subcellular location

Nucleus Probable.

Sequence similarities

Belongs to the SNF2/RAD54 helicase family.

Contains 1 helicase ATP-binding domain.

Contains 1 helicase C-terminal domain.

Ontologies

Keywords
   Biological processDNA damage
DNA repair
   Cellular componentNucleus
   LigandATP-binding
DNA-binding
Nucleotide-binding
   Molecular functionHelicase
Hydrolase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processdouble-strand break repair via homologous recombination

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

DNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

DNA translocase activity

Inferred from electronic annotation. Source: Ensembl

helicase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 886886DNA repair and recombination protein RAD54B
PRO_0000074341

Regions

Domain291 – 458168Helicase ATP-binding
Domain627 – 788162Helicase C-terminal
Nucleotide binding304 – 3118ATP Potential
Motif409 – 4124DEGH box

Sequences

Sequence LengthMass (Da)Tools
Q6PFE3 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: A723501E5D5723A6

FASTA88699,338
        10         20         30         40         50         60 
MRRSAAPSQV QGKSFKKTRF IPPGRSNADV SKEITKMSPD PKLFQGAEQS QNDPGVCSSN 

        70         80         90        100        110        120 
PCPSEGIPRE VGDGTRVDPL PPVHSASKEI TESKAQEEEA SSLLKYFSVV WCKASKKKHK 

       130        140        150        160        170        180 
KWEGDAILIV RGRSFTLKDL EGKDIGRGIG YKFKDLENVE EGQTLIIGGK EIEILGTISS 

       190        200        210        220        230        240 
DDFNSGKCFQ HGSGSPAVPS SQAARKCFSN PFKSVCQSTQ AQGKRWNDCR PRHNPCTPNA 

       250        260        270        280        290        300 
LVMPRPDENH QRMFNRHCSP IVDVVIDPHL VHHLRPHQKD GIIFLYECVM GMRAVGKCGA 

       310        320        330        340        350        360 
ILADEMGLGK TLQCISLIWT LQCQGPYGGK PVIKKTLIVT PGSLVNNWRK EFQKWLGSER 

       370        380        390        400        410        420 
IKIFTVDQDH KVEEFINSTF HSVLIISYEM LLRSLDQIKT IPFGLLICDE GHRLKNSSIK 

       430        440        450        460        470        480 
TTTALSSLSC EKTVILTGTP VQNDLQEFFA LVDFVNPGIL GSLSSYRKIY EEPIIISREP 

       490        500        510        520        530        540 
SSSKEERELG ERRATELTRL TGRFILRRTQ EVINKYLPPK IENVVFCRPG ALQIELYRKL 

       550        560        570        580        590        600 
LRSQSVRFCL QGLLENSAHL ICIGALKKLC NHPCLLFSSV KGKEFSSSCE ENEERNLCQG 

       610        620        630        640        650        660 
LLSVFPAGYN PLQFSEEESG KLQVLVKLLA VIHELRPTEK VILVSNYRQT LNVLEEVCKR 

       670        680        690        700        710        720 
HGYACARLDG QTPVSQRQHI VDSFNSKYST DFIFLLSSKA GGVGLNLIGG SHLILYDIDW 

       730        740        750        760        770        780 
NPATDIQAMS RVWRDGQKHP VHIYRLLTTG TIEEKIYQRQ ISKQGLSGAV VDLTRSSEHI 

       790        800        810        820        830        840 
QFSVEELKNL FTLHESSHCV THDLLDCECT GEKGHTEDAS EGPVASRQCQ FGPQKSDALR 

       850        860        870        880 
PLSMSQLKQW KHFSGDHLNL PDPFLERIRE NVSFFFQNIT NQAPAV 

« Hide

References

« Hide 'large scale' references
[1]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.
Tissue: Embryonic brain.
[3]"Somatic hypermutation does not require Rad54 and Rad54B-mediated homologous recombination."
Bross L., Wesoly J., Buerstedde J.-M., Kanaar R., Jacobs H.
Eur. J. Immunol. 33:352-357(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL732538 Genomic DNA. Translation: CAM18403.1.
BC057604 mRNA. Translation: AAH57604.1.
RefSeqNP_001034645.1. NM_001039556.3.
UniGeneMm.491200.

3D structure databases

ProteinModelPortalQ6PFE3.
SMRQ6PFE3. Positions 228-883.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteQ6PFE3.

Proteomic databases

PRIDEQ6PFE3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000070755; ENSMUSP00000066977; ENSMUSG00000078773.
GeneID623474.
KEGGmmu:623474.
UCSCuc008rzs.2. mouse.

Organism-specific databases

CTD25788.
MGIMGI:3605986. Rad54b.

Phylogenomic databases

eggNOGCOG0553.
GeneTreeENSGT00550000074619.
HOGENOMHOG000204521.
HOVERGENHBG058654.
InParanoidA2AIV0.
KOK10877.
OMAVYHLRPH.
OrthoDBEOG7DJSKN.
PhylomeDBQ6PFE3.
TreeFamTF101223.

Gene expression databases

ArrayExpressQ6PFE3.
BgeeQ6PFE3.
GenevestigatorQ6PFE3.

Family and domain databases

Gene3D3.40.50.300. 2 hits.
InterProIPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000330. SNF2_N.
[Graphical view]
PfamPF00271. Helicase_C. 1 hit.
PF00176. SNF2_N. 1 hit.
[Graphical view]
SMARTSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 2 hits.
PROSITEPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio417142.
PROQ6PFE3.
SOURCESearch...

Entry information

Entry nameRA54B_MOUSE
AccessionPrimary (citable) accession number: Q6PFE3
Secondary accession number(s): A2AIV0
Entry history
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: July 5, 2004
Last modified: April 16, 2014
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot