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Protein

DNA repair and recombination protein RAD54B

Gene

Rad54b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in DNA repair and mitotic recombination. May play an active role in recombination processes in concert with other members of the RAD52 epistasis group.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi304 – 3118ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA binding Source: UniProtKB-KW
  3. DNA translocase activity Source: Ensembl
  4. helicase activity Source: UniProtKB-KW

GO - Biological processi

  1. double-strand break repair via homologous recombination Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA repair and recombination protein RAD54B (EC:3.6.4.-)
Alternative name(s):
RAD54 homolog B
Gene namesi
Name:Rad54b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:3605986. Rad54b.

Subcellular locationi

Nucleus Curated

GO - Cellular componenti

  1. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 886886DNA repair and recombination protein RAD54BPRO_0000074341Add
BLAST

Proteomic databases

MaxQBiQ6PFE3.
PRIDEiQ6PFE3.

PTM databases

PhosphoSiteiQ6PFE3.

Expressioni

Gene expression databases

BgeeiQ6PFE3.
ExpressionAtlasiQ6PFE3. baseline and differential.
GenevestigatoriQ6PFE3.

Interactioni

Subunit structurei

Interacts with RAD51 through the NH2-terminal domain.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ6PFE3.
SMRiQ6PFE3. Positions 300-813.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini291 – 458168Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini627 – 788162Helicase C-terminalPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi409 – 4124DEGH box

Sequence similaritiesi

Belongs to the SNF2/RAD54 helicase family.Curated
Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0553.
GeneTreeiENSGT00780000122039.
HOGENOMiHOG000204521.
HOVERGENiHBG058654.
InParanoidiQ6PFE3.
KOiK10877.
OMAiVYHLRPH.
OrthoDBiEOG7DJSKN.
PhylomeDBiQ6PFE3.
TreeFamiTF101223.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000330. SNF2_N.
[Graphical view]
PfamiPF00271. Helicase_C. 1 hit.
PF00176. SNF2_N. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6PFE3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRRSAAPSQV QGKSFKKTRF IPPGRSNADV SKEITKMSPD PKLFQGAEQS
60 70 80 90 100
QNDPGVCSSN PCPSEGIPRE VGDGTRVDPL PPVHSASKEI TESKAQEEEA
110 120 130 140 150
SSLLKYFSVV WCKASKKKHK KWEGDAILIV RGRSFTLKDL EGKDIGRGIG
160 170 180 190 200
YKFKDLENVE EGQTLIIGGK EIEILGTISS DDFNSGKCFQ HGSGSPAVPS
210 220 230 240 250
SQAARKCFSN PFKSVCQSTQ AQGKRWNDCR PRHNPCTPNA LVMPRPDENH
260 270 280 290 300
QRMFNRHCSP IVDVVIDPHL VHHLRPHQKD GIIFLYECVM GMRAVGKCGA
310 320 330 340 350
ILADEMGLGK TLQCISLIWT LQCQGPYGGK PVIKKTLIVT PGSLVNNWRK
360 370 380 390 400
EFQKWLGSER IKIFTVDQDH KVEEFINSTF HSVLIISYEM LLRSLDQIKT
410 420 430 440 450
IPFGLLICDE GHRLKNSSIK TTTALSSLSC EKTVILTGTP VQNDLQEFFA
460 470 480 490 500
LVDFVNPGIL GSLSSYRKIY EEPIIISREP SSSKEERELG ERRATELTRL
510 520 530 540 550
TGRFILRRTQ EVINKYLPPK IENVVFCRPG ALQIELYRKL LRSQSVRFCL
560 570 580 590 600
QGLLENSAHL ICIGALKKLC NHPCLLFSSV KGKEFSSSCE ENEERNLCQG
610 620 630 640 650
LLSVFPAGYN PLQFSEEESG KLQVLVKLLA VIHELRPTEK VILVSNYRQT
660 670 680 690 700
LNVLEEVCKR HGYACARLDG QTPVSQRQHI VDSFNSKYST DFIFLLSSKA
710 720 730 740 750
GGVGLNLIGG SHLILYDIDW NPATDIQAMS RVWRDGQKHP VHIYRLLTTG
760 770 780 790 800
TIEEKIYQRQ ISKQGLSGAV VDLTRSSEHI QFSVEELKNL FTLHESSHCV
810 820 830 840 850
THDLLDCECT GEKGHTEDAS EGPVASRQCQ FGPQKSDALR PLSMSQLKQW
860 870 880
KHFSGDHLNL PDPFLERIRE NVSFFFQNIT NQAPAV
Length:886
Mass (Da):99,338
Last modified:July 4, 2004 - v1
Checksum:iA723501E5D5723A6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL732538 Genomic DNA. Translation: CAM18403.1.
BC057604 mRNA. Translation: AAH57604.1.
CCDSiCCDS17970.1.
RefSeqiNP_001034645.1. NM_001039556.3.
UniGeneiMm.491200.

Genome annotation databases

EnsembliENSMUST00000070755; ENSMUSP00000066977; ENSMUSG00000078773.
GeneIDi623474.
KEGGimmu:623474.
UCSCiuc008rzs.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL732538 Genomic DNA. Translation: CAM18403.1.
BC057604 mRNA. Translation: AAH57604.1.
CCDSiCCDS17970.1.
RefSeqiNP_001034645.1. NM_001039556.3.
UniGeneiMm.491200.

3D structure databases

ProteinModelPortaliQ6PFE3.
SMRiQ6PFE3. Positions 300-813.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

PhosphoSiteiQ6PFE3.

Proteomic databases

MaxQBiQ6PFE3.
PRIDEiQ6PFE3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000070755; ENSMUSP00000066977; ENSMUSG00000078773.
GeneIDi623474.
KEGGimmu:623474.
UCSCiuc008rzs.2. mouse.

Organism-specific databases

CTDi25788.
MGIiMGI:3605986. Rad54b.

Phylogenomic databases

eggNOGiCOG0553.
GeneTreeiENSGT00780000122039.
HOGENOMiHOG000204521.
HOVERGENiHBG058654.
InParanoidiQ6PFE3.
KOiK10877.
OMAiVYHLRPH.
OrthoDBiEOG7DJSKN.
PhylomeDBiQ6PFE3.
TreeFamiTF101223.

Miscellaneous databases

ChiTaRSiRad54b. mouse.
NextBioi417142.
PROiQ6PFE3.
SOURCEiSearch...

Gene expression databases

BgeeiQ6PFE3.
ExpressionAtlasiQ6PFE3. baseline and differential.
GenevestigatoriQ6PFE3.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000330. SNF2_N.
[Graphical view]
PfamiPF00271. Helicase_C. 1 hit.
PF00176. SNF2_N. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Embryonic brain.
  3. "Somatic hypermutation does not require Rad54 and Rad54B-mediated homologous recombination."
    Bross L., Wesoly J., Buerstedde J.-M., Kanaar R., Jacobs H.
    Eur. J. Immunol. 33:352-357(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiRA54B_MOUSE
AccessioniPrimary (citable) accession number: Q6PFE3
Secondary accession number(s): A2AIV0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 15, 2004
Last sequence update: July 4, 2004
Last modified: March 31, 2015
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.