Nuclear pore complex protein Nup98-Nup96 precursor

Sequence or UniProt identifier

>sp|Q6PFD9|NUP98_MOUSE Nuclear pore complex protein Nup98-Nup96 OS=Mus musculus GN=Nup98 PE=1 SV=2 MFNKSFGTPFGGSTGGFGTTSTFGQNTGFGTTSGGAFGTSAFGSSNNTGGLFGNSQTKPG GLFGTSSFSQPATSTSTGFGFGTSTGTSNSLFGTASTGTSLFSSQNNAFAQNKPTGFGNF GTSTSSGGLFGTTNTTSNPFGSTSGSLFGPSSFTAAPTGTTIKFNPPTGTDTMVKAGVST NISTKHQCITAMKEYESKSLEELRLEDYQANRKGPQNQVGGGTTAGLFGSSPATSSATGL FSSSTTNSAFSYGQNKTAFGTSTTGFGTNPGGLFGQQNQQTTSLFSKPFGQATTTPNTGF SFGNTSTLGQPSTNTMGLFGVTQASQPGGLFGTATNTSTGTAFGTGTGLFGQPNTGFGAV GSTLFGNNKLTTFGTSTTSAPSFGTTSGGLFGNKPTLTLGTNTNTSNFGFGTNNSGSSIF GSKPAAGTLGTGLGTGFGTALGAGQASLFGNNQPKIGGPLGTGAFGAPGFNTSTAILGFG APQAPVALTDPNASAAQQAVLQQHLNSLTYSPFGDSPLFRNPMSDPKKKEERLKPTNPAA QKALTTPTHYKLTPRPATRVRPKALQTTGTAKSHLFDGLDDDEPSLANGAFMPKKSIKKL VLKNLNNSNLFSPVNHDSEDLASPSEYPENGERFSFLSKPVDENNQQDGEDDSLVSRFYT NPIAKPIPQTPESVGNKNNSSSNVEDTIVALNMRAALRNGLEGSSEETSFHDESLQDDRE EIENNAYHIHPAGIVLTKVGYYTIPSMDDLAKITNEKGECIVSDFTIGRKGYGSIYFEGD VNLTNLNLDDIVHIRRKEVIVYVDDNQKPPVGEGLNRKAEVTLDGVWPTDKTSRCLIKSP DRLADINYEGRLEAVSRKQGAQFKEYRPETGSWVFKVSHFSKYGLQDSDEEEEEHPPKTT SKKLKTAPLPPAGQATTFQMTLNGKPAPPPQSQSPEVEQLGRVVELDSDMVDITQEPVPD SVLEESVPEDQEPVSASTHIASSLGINPHVLQIMKASLLVDEEDVDAMDQRFGHIPSKGE TVQEICSPRLPISASHSSKSRSIVGGLLQSKFASGTFLSPSASVQECRTPRTSSRMNIPS TSPWSVPLPLATVFTVPSPAPEVQLKTVGIRRQPGLVPLEKSITYGKGKLLMDMALFMGR SFRVGWGPNWTLANSGEQLHGSHELENHQVADSMEYGFLPNPVAVKSLSESPFKVHLEKL GLRQRKLDEDLQLYQTPLELKLKHSTVHVDELCPLIVPNPGVSVIHDYADWVKDSPGDFL ELPIVKHWSLTWTLCEALWGHLKELDGQLDEPSEYIQTLERRRAFSRWLSHTAAPQIEEE VSLTRRDSPVEAVFSYLTGSRISGACCLAQQSGDHRLALLLSQLVGSQSVRELLTMQLAD WHQLQADSFIHDERLRIFALLAGKPVWQLSEQKQINVCSQLDWKRTLAIHLWYLLPPTAS ISRALSMYEEAFQNTPEGDKYACSPLPSYLEGCGCMVEEEKDSRRPLQDVCFHLLKLYSD RHYELNQLLEPRSITADPLDYRLSWHLWEVLRALNYTHLSEQCEGVLQASYAGQLESEGL WEWAIFVFLHIDNSGMREKAVRELLTRHCQLSETPESWAKEAFLTQKLCVPAEWIHEAKA VRAHMESNKHLEALYLFKAGHWNRCHKLVIRHLASDAIINENYDYLKGFLEDLAPPERSS LIQDWETSGLVYLDYIRVIEMLHRIQQVDCSGYELEHLHTKVTSLCNRIEQIPCYNAKDR LAQSDMAKRVANLLRVVLSLQHAPDATSNSTPDPQRVPLRLLAPHIGRLPMPEDYALEEL RGLTQSYLRELTVGSQ

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Q6PFD9 (NUP98_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 68. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Nuclear pore complex protein Nup98-Nup96

Cleaved into the following 2 chains:

Nuclear pore complex protein Nup98
Alternative name(s):
98 kDa nucleoporin
Nucleoporin Nup98
Short name=Nup98
Nuclear pore complex protein Nup96
Alternative name(s):
96 kDa nucleoporin
Nucleoporin Nup96
Short name=Nup96

Gene names

Name:

Nup98

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	1816 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays a role in the nuclear pore complex (NPC) assembly and/or maintenance. Involved in the bidirectional transport across the NPC. May anchor NUP153 and TPR to the NPC By similarity.
Subunit structure	Part of the nuclear pore complex (NPC). Nup98 interacts directly with Nup96. Nup96 is part of the Nup160 subcomplex in the nuclear pore which is composed of NUP160, NUP133, NUP107 and Nup96. This complex plays a role in RNA export and in tethering Nup98 and NUP153 to the nucleus. Interacts with RAE1. Does not interact with TPR By similarity. Nup98 interacts directly with NUP88 and NUP214 (orthologs of yeast Nup82 and Nup159), subunits of the cytoplasmic filaments of the NPC. Ref.3
Subcellular location	Nucleus By similarity. Nucleus › nuclear pore complex By similarity. Nucleus membrane; Peripheral membrane protein; Nucleoplasmic side By similarity. Note: Nup96 is localized to the nucleoplasmic side of the nuclear pore complex (NPC), at or near the nucleoplasmic basket. Dissociates from the dissasembled NPC structure early during prophase of mitosis. Colocalized with NUP153 and TPR to the nuclear basket of NPC. Detected in diffuse and discrete intranuclear foci By similarity.
Domain	Contains G-L-F-G repeats. The FG repeat domains have a direct role in the transport By similarity.
Post-translational modification	Autoproteolytically cleaved to yield Nup98 and Nup96 or Nup98 only, respectively. Cleaved Nup98 is necessary for the targeting of Nup98 to the nuclear pore and the interaction with Nup96 By similarity.
Sequence similarities	Belongs to the nucleoporin GLFG family. Contains 1 peptidase S59 domain.
Sequence caution	The sequence AAH50911.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence. The sequence AAH57608.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence. The sequence AAI12913.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence.

Ontologies

Keywords
Biological process	Protein transport Translocation Transport mRNA transport
Cellular component	Membrane Nuclear pore complex Nucleus
Domain	Repeat
PTM	Acetylation Autocatalytic cleavage Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA replication Inferred from electronic annotation. Source: Compara mRNA transport Inferred from electronic annotation. Source: UniProtKB-KW protein import into nucleus, docking Inferred from electronic annotation. Source: Compara
Cellular_component	kinetochore Inferred from electronic annotation. Source: Compara nuclear membrane Inferred from sequence or structural similarity. Source: UniProtKB nuclear pore Inferred from electronic annotation. Source: UniProtKB-SubCell nucleoplasm Inferred from electronic annotation. Source: Compara
Molecular_function	peptide binding Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 880

880

Nuclear pore complex protein Nup98

PRO_0000421837

Chain

881 – 1816

936

Nuclear pore complex protein Nup96

PRO_0000421838

Regions

Domain

738 – 880

143

Peptidase S59

Region

1 – 156

156

FG repeats 1

Region

157 – 213

GLEBS; interaction with RAE1 By similarity

Region

214 – 480

267

FG repeats 2

Compositional bias

7 – 481

475

Gly/Thr-rich

Compositional bias

890 – 894

Poly-Glu

Amino acid modifications

Modified residue

524

Phosphoserine By similarity

Modified residue

603

N6-acetyllysine By similarity

Modified residue

608

Phosphoserine By similarity

Modified residue

612

Phosphoserine By similarity

Modified residue

618

Phosphoserine By similarity

Modified residue

623

Phosphoserine By similarity

Modified residue

625

Phosphoserine By similarity

Modified residue

670

Phosphothreonine By similarity

Modified residue

673

Phosphoserine By similarity

Modified residue

681

Phosphoserine By similarity

Modified residue

839

Phosphoserine By similarity

Modified residue

888

Phosphoserine By similarity

Modified residue

934

Phosphoserine By similarity

Modified residue

1027

Phosphoserine By similarity

Modified residue

1042

Phosphoserine By similarity

Modified residue

1059

Phosphoserine By similarity

Modified residue

1069

Phosphothreonine By similarity

Experimental info

Mutagenesis

831

K → A: Reduces interaction with NUP88. Ref.3

Sequences

Sequence

Length

Mass (Da)

Tools

Q6PFD9 [UniParc].

Last modified April 3, 2013. Version 2.
Checksum: CEB86B4A447E9750
Show »

FASTA

1,816

197,241

        10         20         30         40         50         60 
MFNKSFGTPF GGSTGGFGTT STFGQNTGFG TTSGGAFGTS AFGSSNNTGG LFGNSQTKPG 

        70         80         90        100        110        120 
GLFGTSSFSQ PATSTSTGFG FGTSTGTSNS LFGTASTGTS LFSSQNNAFA QNKPTGFGNF 

       130        140        150        160        170        180 
GTSTSSGGLF GTTNTTSNPF GSTSGSLFGP SSFTAAPTGT TIKFNPPTGT DTMVKAGVST 

       190        200        210        220        230        240 
NISTKHQCIT AMKEYESKSL EELRLEDYQA NRKGPQNQVG GGTTAGLFGS SPATSSATGL 

       250        260        270        280        290        300 
FSSSTTNSAF SYGQNKTAFG TSTTGFGTNP GGLFGQQNQQ TTSLFSKPFG QATTTPNTGF 

       310        320        330        340        350        360 
SFGNTSTLGQ PSTNTMGLFG VTQASQPGGL FGTATNTSTG TAFGTGTGLF GQPNTGFGAV 

       370        380        390        400        410        420 
GSTLFGNNKL TTFGTSTTSA PSFGTTSGGL FGNKPTLTLG TNTNTSNFGF GTNNSGSSIF 

       430        440        450        460        470        480 
GSKPAAGTLG TGLGTGFGTA LGAGQASLFG NNQPKIGGPL GTGAFGAPGF NTSTAILGFG 

       490        500        510        520        530        540 
APQAPVALTD PNASAAQQAV LQQHLNSLTY SPFGDSPLFR NPMSDPKKKE ERLKPTNPAA 

       550        560        570        580        590        600 
QKALTTPTHY KLTPRPATRV RPKALQTTGT AKSHLFDGLD DDEPSLANGA FMPKKSIKKL 

       610        620        630        640        650        660 
VLKNLNNSNL FSPVNHDSED LASPSEYPEN GERFSFLSKP VDENNQQDGE DDSLVSRFYT 

       670        680        690        700        710        720 
NPIAKPIPQT PESVGNKNNS SSNVEDTIVA LNMRAALRNG LEGSSEETSF HDESLQDDRE 

       730        740        750        760        770        780 
EIENNAYHIH PAGIVLTKVG YYTIPSMDDL AKITNEKGEC IVSDFTIGRK GYGSIYFEGD 

       790        800        810        820        830        840 
VNLTNLNLDD IVHIRRKEVI VYVDDNQKPP VGEGLNRKAE VTLDGVWPTD KTSRCLIKSP 

       850        860        870        880        890        900 
DRLADINYEG RLEAVSRKQG AQFKEYRPET GSWVFKVSHF SKYGLQDSDE EEEEHPPKTT 

       910        920        930        940        950        960 
SKKLKTAPLP PAGQATTFQM TLNGKPAPPP QSQSPEVEQL GRVVELDSDM VDITQEPVPD 

       970        980        990       1000       1010       1020 
SVLEESVPED QEPVSASTHI ASSLGINPHV LQIMKASLLV DEEDVDAMDQ RFGHIPSKGE 

      1030       1040       1050       1060       1070       1080 
TVQEICSPRL PISASHSSKS RSIVGGLLQS KFASGTFLSP SASVQECRTP RTSSRMNIPS 

      1090       1100       1110       1120       1130       1140 
TSPWSVPLPL ATVFTVPSPA PEVQLKTVGI RRQPGLVPLE KSITYGKGKL LMDMALFMGR 

      1150       1160       1170       1180       1190       1200 
SFRVGWGPNW TLANSGEQLH GSHELENHQV ADSMEYGFLP NPVAVKSLSE SPFKVHLEKL 

      1210       1220       1230       1240       1250       1260 
GLRQRKLDED LQLYQTPLEL KLKHSTVHVD ELCPLIVPNP GVSVIHDYAD WVKDSPGDFL 

      1270       1280       1290       1300       1310       1320 
ELPIVKHWSL TWTLCEALWG HLKELDGQLD EPSEYIQTLE RRRAFSRWLS HTAAPQIEEE 

      1330       1340       1350       1360       1370       1380 
VSLTRRDSPV EAVFSYLTGS RISGACCLAQ QSGDHRLALL LSQLVGSQSV RELLTMQLAD 

      1390       1400       1410       1420       1430       1440 
WHQLQADSFI HDERLRIFAL LAGKPVWQLS EQKQINVCSQ LDWKRTLAIH LWYLLPPTAS 

      1450       1460       1470       1480       1490       1500 
ISRALSMYEE AFQNTPEGDK YACSPLPSYL EGCGCMVEEE KDSRRPLQDV CFHLLKLYSD 

      1510       1520       1530       1540       1550       1560 
RHYELNQLLE PRSITADPLD YRLSWHLWEV LRALNYTHLS EQCEGVLQAS YAGQLESEGL 

      1570       1580       1590       1600       1610       1620 
WEWAIFVFLH IDNSGMREKA VRELLTRHCQ LSETPESWAK EAFLTQKLCV PAEWIHEAKA 

      1630       1640       1650       1660       1670       1680 
VRAHMESNKH LEALYLFKAG HWNRCHKLVI RHLASDAIIN ENYDYLKGFL EDLAPPERSS 

      1690       1700       1710       1720       1730       1740 
LIQDWETSGL VYLDYIRVIE MLHRIQQVDC SGYELEHLHT KVTSLCNRIE QIPCYNAKDR 

      1750       1760       1770       1780       1790       1800 
LAQSDMAKRV ANLLRVVLSL QHAPDATSNS TPDPQRVPLR LLAPHIGRLP MPEDYALEEL 

      1810 
RGLTQSYLRE LTVGSQ

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Brain.
[3]	"Molecular basis for the anchoring of proto-oncoprotein Nup98 to the cytoplasmic face of the nuclear pore complex." Stuwe T., von Borzyskowski L.S., Davenport A.M., Hoelz A. J. Mol. Biol. 419:330-346(2012) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.40 ANGSTROMS) OF 732-880 IN COMPLEX WITH YEAST NUP82 AND NUP159, INTERACTION WITH NUP88, MUTAGENESIS OF LYS-831, SUBUNIT.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AC118592 Genomic DNA. No translation available.
BC050911 mRNA. Translation: AAH50911.1. Sequence problems.
BC057608 mRNA. Translation: AAH57608.1. Sequence problems.
BC078630 mRNA. Translation: AAH78630.1.
BC112912 mRNA. Translation: AAI12913.1. Sequence problems.

IPI

IPI00464141.

RefSeq

NP_075355.1. NM_022979.1.

UniGene

Mm.439800.
Mm.486276.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3TKN	X-ray	3.40	C/F/I	732-880	[»]

ProteinModelPortal

Q6PFD9.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q6PFD9. 11 interactions.

Protein family/group databases

MEROPS

S59.001.

Proteomic databases

PRIDE

Q6PFD9.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMUST00000070165; ENSMUSP00000068530; ENSMUSG00000063550.

GeneID

269966.

KEGG

mmu:269966.

UCSC

uc009iqy.1. mouse.

Organism-specific databases

CTD

4928.

MGI

MGI:109404. Nup98.

Phylogenomic databases

GeneTree

ENSGT00550000074799.

HOGENOM

HOG000044579.

HOVERGEN

HBG052702.

K14297.

OMA

LESISAM.

Gene expression databases

ArrayExpress

Q6PFD9.

Genevestigator

Q6PFD9.

Family and domain databases

InterPro

IPR021967. Nup96.
IPR007230. Peptidase_S59.
[Graphical view]

Pfam

PF04096. Nucleoporin2. 1 hit.
PF12110. Nup96. 1 hit.
[Graphical view]

SUPFAM

SSF82215. Peptidase_S59. 1 hit.

PROSITE

PS51434. NUP_C. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

393133.

SOURCE

Search...

Entry information

Entry name

NUP98_MOUSE

Accession

Primary (citable) accession number: Q6PFD9
Secondary accession number(s): Q68G59

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 3, 2013
Last sequence update:	April 3, 2013
Last modified:	May 1, 2013
This is version 68 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

Peptidase families

Classification of peptidase families and list of entries

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Cleaved into the following 2 chains:

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents