Q6PFD5 (DLGP3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Disks large-associated protein 3
Short name=DAP-3

Alternative name(s):
PSD-95/SAP90-binding protein 3
SAP90/PSD-95-associated protein 3
Short name=SAPAP3

Gene names

Name:	Dlgap3
Synonyms:	Dap3

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	977 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May play a role in the molecular organization of synapses and neuronal cell signaling. Could be an adapter protein linking ion channel to the subsynaptic cytoskeleton. May induce enrichment of PSD-95/SAP90 at the plasma membrane.
Subunit structure	Interacts with DLG4/PSD-95 By similarity.
Subcellular location	Cell membrane; Peripheral membrane protein. Cell junction › synapse › postsynaptic cell membrane › postsynaptic density. Cell junction › synapse. Note: Postsynaptic density of neuronal cells. Ref.1
Tissue specificity	Highly expressed in central and peripherical nervous system (at protein level). Ref.1
Sequence similarities	Belongs to the SAPAP family.

Ontologies

Keywords
Cellular component	Cell junction Cell membrane Membrane Postsynaptic cell membrane Synapse
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cell-cell signaling Inferred from electronic annotation. Source: InterPro
Cellular component	cell junction Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell postsynaptic density Inferred from electronic annotation. Source: UniProtKB-SubCell postsynaptic membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	beta-amyloid binding Inferred from direct assay. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 977	977	Disks large-associated protein 3		PRO_0000174295
Regions
	Compositional bias	222 – 233	12	Poly-His
Amino acid modifications
	Modified residue	58	1	Phosphoserine Ref.3 Ref.4
	Modified residue	394	1	Phosphoserine Ref.3
	Modified residue	404	1	Phosphoserine Ref.3
	Modified residue	407	1	Phosphoserine Ref.3
	Modified residue	410	1	Phosphoserine Ref.3
	Modified residue	414	1	Phosphoserine Ref.3
	Modified residue	500	1	Phosphoserine Ref.4
	Modified residue	930	1	Phosphoserine Ref.4
	Modified residue	965	1	Phosphoserine Ref.4
Experimental info
	Sequence conflict	436	1	N → S in AAO89219. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q6PFD5 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 26665D623D49E4C3	FASTA	977	105,873
	10 20 30 40 50 60 MRGYHGDRGS HPRPARFADQ QHMDVGPAAR APYLLGSREA FSTEPRFCAP RAGLGHLSPE 70 80 90 100 110 120 GPLSLSEGPS SVGPEGGPGG VGAGGGSSTF PRMYPGQGPF DTCEDCVGHP QGKGATRLPP 130 140 150 160 170 180 TLLDQFEKQL PVQQDGFHTL PYQRGPAGPG PGPGSGAAPE ARSESPSRIR HLVHSVQKLF 190 200 210 220 230 240 AKSHSLEAPG KRDYNGPKAD GRGSSGGDSY SGPGSGGTPT SHHHHHHHHH HHHQSRHGKR 250 260 270 280 290 300 SKSKDRKGDG RHQTKATGWW SSDDNLDSDS GFLGGRPPGE PGGPFCLDAP DGSYRDLSFK 310 320 330 340 350 360 GRSGGSEGRC LACTGMSMSL DGQSVKRSAW HTMMVSQGRD GYPGAGPGKG LLGPETKAKA 370 380 390 400 410 420 RTYHYLQVPQ DDWGGYPTGG KDGEIPCRRM RSGSYIKAMG DEESGDSDGS PKTSPKALAR 430 440 450 460 470 480 RFASRRSSSV DTARINCCVP PRIHPRSSIP GYSRSLTTGQ LSEEFNQQLE AVCGSVFGEL 490 500 510 520 530 540 ESQAVDALDL PGCFRMRSHS YLRAIQAGCS QDDDCLPLLA APASVSGRPG SSFNFRKAPP 550 560 570 580 590 600 PIPPGSQAPP RISITAQSST DSAHESFTAA EGPARRCSSA DGLDGPTMGA RTLELAPVPP 610 620 630 640 650 660 RASPKPPTLI IKTIPGREEL RSLARQRKWR PSIGVQVETI SDSDTENRSR REFHSIGVQV 670 680 690 700 710 720 EEDKRRARFK RSNSVTAGVQ ADLELEGLAG LATVATEDKA LQFGRSFQRH ASEPQPGPRA 730 740 750 760 770 780 PTYSVFRTVH TQGQWAYREG YPLPYEPPAT DGSPGPTPVP APGPGSGRRD SWMERGSRSL 790 800 810 820 830 840 PDSGRTSPCP RDGEWFIKML RAEVEKLEHW CQQMEREAED YELPEEILEK IRSAVGSTQL 850 860 870 880 890 900 LLSQKVQQFF RLCQQSLDPT AFPVPTFQDL AGFWDLLQLS IEDVTLKFLE LQQLKANSWK 910 920 930 940 950 960 LLEPKEEKKV PPPIPKKPSR GRGVPVKERS LDSVDRQRQE ARKRLLAAKR AASFRHSSAT 970 ESADSIEIYI PEAQTRL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Differential mRNA expression and protein localization of the SAP90/PSD-95-associated proteins (SAPAPs) in the nervous system of the mouse." Welch J.M., Wang D., Feng G. J. Comp. Neurol. 472:24-39(2004) [PubMed: 15024750] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, SUBCELLULAR LOCATION. Strain: ICR.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6.
[3]	"Proteomic analysis of in vivo phosphorylated synaptic proteins." Collins M.O., Yu L., Coba M.P., Husi H., Campuzano I., Blackstock W.P., Choudhary J.S., Grant S.G. J. Biol. Chem. 280:5972-5982(2005) [PubMed: 15572359] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-58; SER-394; SER-404; SER-407; SER-410 AND SER-414, MASS SPECTROMETRY. Tissue: Forebrain.
[4]	"Comprehensive identification of phosphorylation sites in postsynaptic density preparations." Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L. Mol. Cell. Proteomics 5:914-922(2006) [PubMed: 16452087] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-58; SER-500; SER-930 AND SER-965, MASS SPECTROMETRY. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY243848 mRNA. Translation: AAO89219.2. BC057615 mRNA. Translation: AAH57615.1. BC058433 mRNA. Translation: AAH58433.1.
IPI	IPI00134997.
RefSeq	NP_941020.1. NM_198618.4.
UniGene	Mm.331885.
3D structure databases
ProteinModelPortal	Q6PFD5.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q6PFD5. 1 interaction.
MINT	MINT-4789697.
STRING	Q6PFD5.
PTM databases
PhosphoSite	Q6PFD5.
Proteomic databases
PRIDE	Q6PFD5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000046659; ENSMUSP00000039724; ENSMUSG00000042388. ENSMUST00000106094; ENSMUSP00000101700; ENSMUSG00000042388.
GeneID	242667.
KEGG	mmu:242667.
Organism-specific databases
CTD	58512.
MGI	MGI:3039563. Dlgap3.
Phylogenomic databases
GeneTree	ENSGT00550000074473.
HOGENOM	HBG444624.
HOVERGEN	HBG018957.
InParanoid	Q6PFD5.
OMA	GFHTLPY.
OrthoDB	EOG4STS3Z.
PhylomeDB	Q6PFD5.
Gene expression databases
ArrayExpress	Q6PFD5.
Bgee	Q6PFD5.
CleanEx	MM_DAP3.
Genevestigator	Q6PFD5.
GermOnline	ENSMUSG00000042388. Mus musculus.
Family and domain databases
InterPro	IPR005026. GKAP. [Graphical view]
Pfam	PF03359. GKAP. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	385480.
SOURCE	Search...

Entry information

Entry name

DLGP3_MOUSE

Accession

Primary (citable) accession number: Q6PFD5
Secondary accession number(s): Q6PDX0, Q6XBF2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	July 5, 2004
Last modified:	October 19, 2011
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program