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Q6PF93

- PK3C3_MOUSE

UniProt

Q6PF93 - PK3C3_MOUSE

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Protein

Phosphatidylinositol 3-kinase catalytic subunit type 3

Gene

Pik3c3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate which plays a key role in initiation and maturation of autophagosomes. Involved in the transport of lysosomal enzyme precursors to lysosomes. Required for the abcission step in cytokinesis. Required for transport from early to late endosomes (By similarity).By similarity

Catalytic activityi

ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 3-phosphate.

Cofactori

Mn2+By similarity

GO - Molecular functioni

  1. 1-phosphatidylinositol-3-kinase activity Source: UniProtKB
  2. ATP binding Source: UniProtKB-KW
  3. protein kinase activity Source: MGI

GO - Biological processi

  1. autophagic vacuole assembly Source: Ensembl
  2. cytokinesis Source: UniProtKB
  3. early endosome to late endosome transport Source: UniProtKB
  4. endosome organization Source: Ensembl
  5. phosphatidylinositol-3-phosphate biosynthetic process Source: GOC
  6. phosphatidylinositol-mediated signaling Source: Ensembl
  7. protein phosphorylation Source: MGI
  8. protein processing Source: Ensembl
  9. regulation of protein secretion Source: Ensembl
  10. response to leucine Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Autophagy, Cell cycle, Cell division

Keywords - Ligandi

ATP-binding, Manganese, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_198970. Synthesis of PIPs at the Golgi membrane.
REACT_198972. Synthesis of PIPs at the late endosome membrane.
REACT_198975. Synthesis of PIPs at the early endosome membrane.
REACT_240139. PI3K Cascade.
REACT_249409. Toll Like Receptor 9 (TLR9) Cascade.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylinositol 3-kinase catalytic subunit type 3 (EC:2.7.1.137)
Short name:
PI3-kinase type 3
Short name:
PI3K type 3
Short name:
PtdIns-3-kinase type 3
Alternative name(s):
Phosphoinositide-3-kinase class 3
Gene namesi
Name:Pik3c3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 18

Organism-specific databases

MGIiMGI:2445019. Pik3c3.

Subcellular locationi

Midbody By similarity. Late endosome By similarity
Note: Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme.1 Publication

GO - Cellular componenti

  1. late endosome Source: UniProtKB
  2. midbody Source: UniProtKB
  3. phagocytic vesicle Source: MGI
  4. phosphatidylinositol 3-kinase complex Source: RefGenome
Complete GO annotation...

Keywords - Cellular componenti

Endosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 887887Phosphatidylinositol 3-kinase catalytic subunit type 3PRO_0000088803Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei261 – 2611PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ6PF93.
PaxDbiQ6PF93.
PRIDEiQ6PF93.

PTM databases

PhosphoSiteiQ6PF93.

Expressioni

Gene expression databases

BgeeiQ6PF93.
CleanExiMM_PIK3C3.
ExpressionAtlasiQ6PF93. baseline and differential.
GenevestigatoriQ6PF93.

Interactioni

Subunit structurei

Heterodimer. This subunit, part of a complex composed of regulatory and catalytic subunits, associates with regulatory subunit PIK3R4 (By similarity). Interacts with AMBRA1. Forms a complex with BECN1, PIK3R4 and either UVRAG and KIAA0226/Rubicon, or with ATG14. In this complex, presence of UVRAG and ATG14 are mutually exclusive (By similarity). Part of a complex composed of PIK3R4 and PIK3CB. Interacts with RAB7A in the presence of PIK3R4. Interacts with BECN1P1/BECN2 (By similarity).By similarity

Protein-protein interaction databases

BioGridi230382. 2 interactions.
DIPiDIP-57222N.
IntActiQ6PF93. 2 interactions.
MINTiMINT-4608698.
STRINGi10090.ENSMUSP00000111479.

Structurei

3D structure databases

ProteinModelPortaliQ6PF93.
SMRiQ6PF93. Positions 12-881.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini35 – 184150C2 PI3K-typePROSITE-ProRule annotationAdd
BLAST
Domaini283 – 520238PIK helicalPROSITE-ProRule annotationAdd
BLAST
Domaini631 – 885255PI3K/PI4KPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the PI3/PI4-kinase family.PROSITE-ProRule annotation
Contains 1 C2 PI3K-type domain.PROSITE-ProRule annotation
Contains 1 PI3K/PI4K domain.PROSITE-ProRule annotation
Contains 1 PIK helical domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5032.
GeneTreeiENSGT00760000119110.
HOGENOMiHOG000174003.
HOVERGENiHBG082145.
InParanoidiQ6PF93.
KOiK00914.
OMAiYLMVEFP.
OrthoDBiEOG7PP55Z.
PhylomeDBiQ6PF93.
TreeFamiTF102032.

Family and domain databases

Gene3Di1.10.1070.11. 1 hit.
1.25.40.70. 2 hits.
2.60.40.150. 1 hit.
InterProiIPR016024. ARM-type_fold.
IPR000008. C2_dom.
IPR011009. Kinase-like_dom.
IPR000403. PI3/4_kinase_cat_dom.
IPR018936. PI3/4_kinase_CS.
IPR002420. PI3K_C2_dom.
IPR008290. PI3K_Vps34.
IPR015433. PI_Kinase.
IPR001263. PInositide-3_kin_accessory_dom.
[Graphical view]
PANTHERiPTHR10048. PTHR10048. 1 hit.
PfamiPF00454. PI3_PI4_kinase. 1 hit.
PF00792. PI3K_C2. 1 hit.
PF00613. PI3Ka. 1 hit.
[Graphical view]
PIRSFiPIRSF000587. PI3K_Vps34. 1 hit.
SMARTiSM00239. C2. 1 hit.
SM00142. PI3K_C2. 1 hit.
SM00145. PI3Ka. 1 hit.
SM00146. PI3Kc. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 2 hits.
SSF49562. SSF49562. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00915. PI3_4_KINASE_1. 1 hit.
PS00916. PI3_4_KINASE_2. 1 hit.
PS50290. PI3_4_KINASE_3. 1 hit.
PS51547. PI3K_C2. 1 hit.
PS51545. PIK_HELICAL. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q6PF93-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGEAEKFHYI YSCDLDINVQ LKIGSLEGKR EQKSYKAVLE DPMLKFSGLY
60 70 80 90 100
QETCSDLYVT CQVFAEGKPL ALPVRTSYKA FSTRWNWNEW LKLPVKYPDL
110 120 130 140 150
PRNAQVALTI WDVYGPGSAV PVGGTTVSLF GKYGMFRQGM HDLKVWPNVE
160 170 180 190 200
ADGSEPTRTP GRTSSTLSED QMSRLAKLTK AHRQGHMVKV DWLDRLTFRE
210 220 230 240 250
IEMINESEKR SSNFMYLMVE FRCVKCDDKE YGIVYYEKDG DESSPILTSF
260 270 280 290 300
ELVKVPDPQM SMENLVESKH HKLARSLRSG PSDHDLKPNA TTRDQLNIIV
310 320 330 340 350
SYPPTKQLTY EEQDLVWKFR YYLTNQEKAL TKFLKCVNWD LPQEAKQALE
360 370 380 390 400
LLGKWKPMDV EDSLELLSSH YTNPTVRRYA VARLRQADDE DLLMYLLQLV
410 420 430 440 450
QALKYENFDD IKNGLEPTKK DSQTSASESL SNSGVSSGDI DSSQIITNPL
460 470 480 490 500
PPVASPPPAS KAKEVSDGEN LEQDLCTFLI SRACKNSTLA NYLYWYVIVE
510 520 530 540 550
CEDQDTQQRD PKTHEMYLNV MRRFSQALLK GDKSVRVMRS LLAAQQTFVD
560 570 580 590 600
RLVHLMKAVQ RESGNRKKKN ERLQALLGDN EKMNLSDVEL IPLPLEPQVK
610 620 630 640 650
IRGIIPETAT LFKSALMPAQ LFFKTEDGGK YPVIFKHGDD LRQDQLILQI
660 670 680 690 700
ISLMDKLLRK ENLDLKLTPY KVLATSTKHG FMQFIQSVPV AEVLDTEGSI
710 720 730 740 750
QNFFRKYAPS ETGPYGISAE VMDTYVKSCA GYCVITYILG VGDRHLDNLL
760 770 780 790 800
LTKTGKLFHI DFGYILGRDP KPLPPPMKLN KEMVEGMGGT QSEQYQEFRK
810 820 830 840 850
QCYTAFLHLR RYSNLILNLF SLMVDANIPD IALEPDKTVK KVQDKFRLDL
860 870 880
SDEEAVHYMQ SLIDESVHAL FAAVVEQIHK FAQYWRK
Length:887
Mass (Da):101,487
Last modified:July 5, 2004 - v1
Checksum:i80E8EF9EF3AF74D7
GO
Isoform 2 (identifier: Q6PF93-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     842-848: VQDKFRL → ISCHRKM
     849-887: Missing.

Note: No experimental confirmation available.

Show »
Length:848
Mass (Da):96,869
Checksum:i26219164AC269CFB
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti455 – 4551Missing in AAH57678. (PubMed:15489334)Curated
Sequence conflicti470 – 4701N → S in AAH57678. (PubMed:15489334)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei842 – 8487VQDKFRL → ISCHRKM in isoform 2. 1 PublicationVSP_016459
Alternative sequencei849 – 88739Missing in isoform 2. 1 PublicationVSP_016460Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC024675 mRNA. Translation: AAH24675.1.
BC057678 mRNA. Translation: AAH57678.1.
CCDSiCCDS37752.1. [Q6PF93-1]
RefSeqiNP_852079.2. NM_181414.5. [Q6PF93-1]
XP_006525902.1. XM_006525839.1. [Q6PF93-1]
UniGeneiMm.194127.

Genome annotation databases

EnsembliENSMUST00000115812; ENSMUSP00000111479; ENSMUSG00000033628. [Q6PF93-1]
GeneIDi225326.
KEGGimmu:225326.
UCSCiuc008ehw.1. mouse. [Q6PF93-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC024675 mRNA. Translation: AAH24675.1 .
BC057678 mRNA. Translation: AAH57678.1 .
CCDSi CCDS37752.1. [Q6PF93-1 ]
RefSeqi NP_852079.2. NM_181414.5. [Q6PF93-1 ]
XP_006525902.1. XM_006525839.1. [Q6PF93-1 ]
UniGenei Mm.194127.

3D structure databases

ProteinModelPortali Q6PF93.
SMRi Q6PF93. Positions 12-881.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 230382. 2 interactions.
DIPi DIP-57222N.
IntActi Q6PF93. 2 interactions.
MINTi MINT-4608698.
STRINGi 10090.ENSMUSP00000111479.

PTM databases

PhosphoSitei Q6PF93.

Proteomic databases

MaxQBi Q6PF93.
PaxDbi Q6PF93.
PRIDEi Q6PF93.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000115812 ; ENSMUSP00000111479 ; ENSMUSG00000033628 . [Q6PF93-1 ]
GeneIDi 225326.
KEGGi mmu:225326.
UCSCi uc008ehw.1. mouse. [Q6PF93-1 ]

Organism-specific databases

CTDi 5289.
MGIi MGI:2445019. Pik3c3.

Phylogenomic databases

eggNOGi COG5032.
GeneTreei ENSGT00760000119110.
HOGENOMi HOG000174003.
HOVERGENi HBG082145.
InParanoidi Q6PF93.
KOi K00914.
OMAi YLMVEFP.
OrthoDBi EOG7PP55Z.
PhylomeDBi Q6PF93.
TreeFami TF102032.

Enzyme and pathway databases

Reactomei REACT_198970. Synthesis of PIPs at the Golgi membrane.
REACT_198972. Synthesis of PIPs at the late endosome membrane.
REACT_198975. Synthesis of PIPs at the early endosome membrane.
REACT_240139. PI3K Cascade.
REACT_249409. Toll Like Receptor 9 (TLR9) Cascade.

Miscellaneous databases

ChiTaRSi Pik3c3. mouse.
NextBioi 377617.
PROi Q6PF93.
SOURCEi Search...

Gene expression databases

Bgeei Q6PF93.
CleanExi MM_PIK3C3.
ExpressionAtlasi Q6PF93. baseline and differential.
Genevestigatori Q6PF93.

Family and domain databases

Gene3Di 1.10.1070.11. 1 hit.
1.25.40.70. 2 hits.
2.60.40.150. 1 hit.
InterProi IPR016024. ARM-type_fold.
IPR000008. C2_dom.
IPR011009. Kinase-like_dom.
IPR000403. PI3/4_kinase_cat_dom.
IPR018936. PI3/4_kinase_CS.
IPR002420. PI3K_C2_dom.
IPR008290. PI3K_Vps34.
IPR015433. PI_Kinase.
IPR001263. PInositide-3_kin_accessory_dom.
[Graphical view ]
PANTHERi PTHR10048. PTHR10048. 1 hit.
Pfami PF00454. PI3_PI4_kinase. 1 hit.
PF00792. PI3K_C2. 1 hit.
PF00613. PI3Ka. 1 hit.
[Graphical view ]
PIRSFi PIRSF000587. PI3K_Vps34. 1 hit.
SMARTi SM00239. C2. 1 hit.
SM00142. PI3K_C2. 1 hit.
SM00145. PI3Ka. 1 hit.
SM00146. PI3Kc. 1 hit.
[Graphical view ]
SUPFAMi SSF48371. SSF48371. 2 hits.
SSF49562. SSF49562. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEi PS00915. PI3_4_KINASE_1. 1 hit.
PS00916. PI3_4_KINASE_2. 1 hit.
PS50290. PI3_4_KINASE_3. 1 hit.
PS51547. PI3K_C2. 1 hit.
PS51545. PIK_HELICAL. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Mammary gland.
  2. Cited for: INTERACTION WITH BECN1 AND AMBRA1.
  3. "The class IA phosphatidylinositol 3-kinase p110-beta subunit is a positive regulator of autophagy."
    Dou Z., Chattopadhyay M., Pan J.-A., Guerriero J.L., Jiang Y.-P., Ballou L.M., Yue Z., Lin R.Z., Zong W.-X.
    J. Cell Biol. 191:827-843(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN A COMPLEX WITH PIK3R4 AND PIK3CB.
  4. Cited for: SUBCELLULAR LOCATION.

Entry informationi

Entry nameiPK3C3_MOUSE
AccessioniPrimary (citable) accession number: Q6PF93
Secondary accession number(s): Q8R3S8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: July 5, 2004
Last modified: November 26, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3