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Q6PF93 (PK3C3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphatidylinositol 3-kinase catalytic subunit type 3

Short name=PI3-kinase type 3
Short name=PI3K type 3
Short name=PtdIns-3-kinase type 3
EC=2.7.1.137
Alternative name(s):
Phosphoinositide-3-kinase class 3
Gene names
Name:Pik3c3
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length887 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate which plays a key role in initiation and maturation of autophagosomes. Involved in the transport of lysosomal enzyme precursors to lysosomes. Required for the abcission step in cytokinesis. Required for transport from early to late endosomes By similarity.

Catalytic activity

ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 3-phosphate.

Cofactor

Manganese By similarity.

Subunit structure

Heterodimer. This subunit, part of a complex composed of regulatory and catalytic subunits, associates with regulatory subunit PIK3R4 By similarity. Interacts with AMBRA1. Forms a complex with BECN1, PIK3R4 and either UVRAG and KIAA0226/Rubicon, or with ATG14. In this complex, presence of UVRAG and ATG14 are mutually exclusive By similarity. Part of a complex composed of PIK3R4 and PIK3CB. Interacts with RAB7A in the presence of PIK3R4. Interacts with BECN1P1/BECN2 By similarity. Ref.2 Ref.3

Subcellular location

Midbody By similarity. Late endosome By similarity. Note: Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme. Ref.4

Sequence similarities

Belongs to the PI3/PI4-kinase family.

Contains 1 C2 PI3K-type domain.

Contains 1 PI3K/PI4K domain.

Contains 1 PIK helical domain.

Ontologies

Keywords
   Biological processAutophagy
Cell cycle
Cell division
   Cellular componentEndosome
   Coding sequence diversityAlternative splicing
   LigandATP-binding
Manganese
Nucleotide-binding
   Molecular functionKinase
Transferase
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processautophagic vacuole assembly

Inferred from electronic annotation. Source: Ensembl

cytokinesis

Inferred from sequence or structural similarity. Source: UniProtKB

early endosome to late endosome transport

Inferred from sequence or structural similarity. Source: UniProtKB

endosome organization

Inferred from electronic annotation. Source: Ensembl

phosphatidylinositol-mediated signaling

Inferred from electronic annotation. Source: Ensembl

protein phosphorylation

Inferred from mutant phenotype PubMed 15010863. Source: MGI

protein processing

Inferred from electronic annotation. Source: Ensembl

regulation of protein secretion

Inferred from electronic annotation. Source: Ensembl

response to leucine

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentlate endosome

Inferred from sequence or structural similarity. Source: UniProtKB

midbody

Inferred from sequence or structural similarity. Source: UniProtKB

phagocytic vesicle

Inferred from direct assay PubMed 22493499. Source: MGI

phosphatidylinositol 3-kinase complex

Inferred from Biological aspect of Ancestor. Source: RefGenome

   Molecular_function1-phosphatidylinositol-3-kinase activity

Inferred from sequence or structural similarity. Source: UniProtKB

ATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

protein kinase activity

Inferred from mutant phenotype PubMed 15010863. Source: MGI

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q6PF93-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q6PF93-2)

The sequence of this isoform differs from the canonical sequence as follows:
     842-848: VQDKFRL → ISCHRKM
     849-887: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 887887Phosphatidylinositol 3-kinase catalytic subunit type 3
PRO_0000088803

Regions

Domain35 – 184150C2 PI3K-type
Domain283 – 520238PIK helical
Domain631 – 885255PI3K/PI4K

Amino acid modifications

Modified residue2611Phosphoserine By similarity

Natural variations

Alternative sequence842 – 8487VQDKFRL → ISCHRKM in isoform 2.
VSP_016459
Alternative sequence849 – 88739Missing in isoform 2.
VSP_016460

Experimental info

Sequence conflict4551Missing in AAH57678. Ref.1
Sequence conflict4701N → S in AAH57678. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 80E8EF9EF3AF74D7

FASTA887101,487
        10         20         30         40         50         60 
MGEAEKFHYI YSCDLDINVQ LKIGSLEGKR EQKSYKAVLE DPMLKFSGLY QETCSDLYVT 

        70         80         90        100        110        120 
CQVFAEGKPL ALPVRTSYKA FSTRWNWNEW LKLPVKYPDL PRNAQVALTI WDVYGPGSAV 

       130        140        150        160        170        180 
PVGGTTVSLF GKYGMFRQGM HDLKVWPNVE ADGSEPTRTP GRTSSTLSED QMSRLAKLTK 

       190        200        210        220        230        240 
AHRQGHMVKV DWLDRLTFRE IEMINESEKR SSNFMYLMVE FRCVKCDDKE YGIVYYEKDG 

       250        260        270        280        290        300 
DESSPILTSF ELVKVPDPQM SMENLVESKH HKLARSLRSG PSDHDLKPNA TTRDQLNIIV 

       310        320        330        340        350        360 
SYPPTKQLTY EEQDLVWKFR YYLTNQEKAL TKFLKCVNWD LPQEAKQALE LLGKWKPMDV 

       370        380        390        400        410        420 
EDSLELLSSH YTNPTVRRYA VARLRQADDE DLLMYLLQLV QALKYENFDD IKNGLEPTKK 

       430        440        450        460        470        480 
DSQTSASESL SNSGVSSGDI DSSQIITNPL PPVASPPPAS KAKEVSDGEN LEQDLCTFLI 

       490        500        510        520        530        540 
SRACKNSTLA NYLYWYVIVE CEDQDTQQRD PKTHEMYLNV MRRFSQALLK GDKSVRVMRS 

       550        560        570        580        590        600 
LLAAQQTFVD RLVHLMKAVQ RESGNRKKKN ERLQALLGDN EKMNLSDVEL IPLPLEPQVK 

       610        620        630        640        650        660 
IRGIIPETAT LFKSALMPAQ LFFKTEDGGK YPVIFKHGDD LRQDQLILQI ISLMDKLLRK 

       670        680        690        700        710        720 
ENLDLKLTPY KVLATSTKHG FMQFIQSVPV AEVLDTEGSI QNFFRKYAPS ETGPYGISAE 

       730        740        750        760        770        780 
VMDTYVKSCA GYCVITYILG VGDRHLDNLL LTKTGKLFHI DFGYILGRDP KPLPPPMKLN 

       790        800        810        820        830        840 
KEMVEGMGGT QSEQYQEFRK QCYTAFLHLR RYSNLILNLF SLMVDANIPD IALEPDKTVK 

       850        860        870        880 
KVQDKFRLDL SDEEAVHYMQ SLIDESVHAL FAAVVEQIHK FAQYWRK 

« Hide

Isoform 2 [UniParc].

Checksum: 26219164AC269CFB
Show »

FASTA84896,869

References

« Hide 'large scale' references
[1]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Tissue: Mammary gland.
[2]"Ambra1 regulates autophagy and development of the nervous system."
Maria Fimia G., Stoykova A., Romagnoli A., Giunta L., Di Bartolomeo S., Nardacci R., Corazzari M., Fuoco C., Ucar A., Schwartz P., Gruss P., Piacentini M., Chowdhury K., Cecconi F.
Nature 447:1121-1125(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH BECN1 AND AMBRA1.
[3]"The class IA phosphatidylinositol 3-kinase p110-beta subunit is a positive regulator of autophagy."
Dou Z., Chattopadhyay M., Pan J.-A., Guerriero J.L., Jiang Y.-P., Ballou L.M., Yue Z., Lin R.Z., Zong W.-X.
J. Cell Biol. 191:827-843(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN A COMPLEX WITH PIK3R4 AND PIK3CB.
[4]"Functional interaction between autophagy and ciliogenesis."
Pampliega O., Orhon I., Patel B., Sridhar S., Diaz-Carretero A., Beau I., Codogno P., Satir B.H., Satir P., Cuervo A.M.
Nature 502:194-200(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC024675 mRNA. Translation: AAH24675.1.
BC057678 mRNA. Translation: AAH57678.1.
RefSeqNP_852079.2. NM_181414.5.
XP_006525902.1. XM_006525839.1.
UniGeneMm.194127.

3D structure databases

ProteinModelPortalQ6PF93.
SMRQ6PF93. Positions 12-881.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid230382. 2 interactions.
IntActQ6PF93. 1 interaction.
MINTMINT-4608698.
STRING10090.ENSMUSP00000111479.

PTM databases

PhosphoSiteQ6PF93.

Proteomic databases

PaxDbQ6PF93.
PRIDEQ6PF93.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000115812; ENSMUSP00000111479; ENSMUSG00000033628. [Q6PF93-1]
GeneID225326.
KEGGmmu:225326.
UCSCuc008ehw.1. mouse. [Q6PF93-1]

Organism-specific databases

CTD5289.
MGIMGI:2445019. Pik3c3.

Phylogenomic databases

eggNOGCOG5032.
GeneTreeENSGT00620000087805.
HOGENOMHOG000174003.
HOVERGENHBG082145.
InParanoidQ6PF93.
KOK00914.
OMAYLMVEFP.
OrthoDBEOG7PP55Z.
PhylomeDBQ6PF93.
TreeFamTF102032.

Gene expression databases

ArrayExpressQ6PF93.
BgeeQ6PF93.
CleanExMM_PIK3C3.
GenevestigatorQ6PF93.

Family and domain databases

Gene3D1.10.1070.11. 1 hit.
1.25.40.70. 2 hits.
2.60.40.150. 1 hit.
InterProIPR016024. ARM-type_fold.
IPR000008. C2_dom.
IPR011009. Kinase-like_dom.
IPR000403. PI3/4_kinase_cat_dom.
IPR018936. PI3/4_kinase_CS.
IPR002420. PI3K_C2_dom.
IPR008290. PI3K_Vps34.
IPR015433. PI_Kinase.
IPR001263. PInositide-3_kin_accessory_dom.
[Graphical view]
PANTHERPTHR10048. PTHR10048. 1 hit.
PfamPF00454. PI3_PI4_kinase. 1 hit.
PF00792. PI3K_C2. 1 hit.
PF00613. PI3Ka. 1 hit.
[Graphical view]
PIRSFPIRSF000587. PI3K_Vps34. 1 hit.
SMARTSM00239. C2. 1 hit.
SM00142. PI3K_C2. 1 hit.
SM00145. PI3Ka. 1 hit.
SM00146. PI3Kc. 1 hit.
[Graphical view]
SUPFAMSSF48371. SSF48371. 2 hits.
SSF49562. SSF49562. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEPS00915. PI3_4_KINASE_1. 1 hit.
PS00916. PI3_4_KINASE_2. 1 hit.
PS50290. PI3_4_KINASE_3. 1 hit.
PS51547. PI3K_C2. 1 hit.
PS51545. PIK_HELICAL. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSPIK3C3. mouse.
NextBio377617.
PROQ6PF93.
SOURCESearch...

Entry information

Entry namePK3C3_MOUSE
AccessionPrimary (citable) accession number: Q6PF93
Secondary accession number(s): Q8R3S8
Entry history
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: July 5, 2004
Last modified: April 16, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot