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Q6PF93

- PK3C3_MOUSE

UniProt

Q6PF93 - PK3C3_MOUSE

Protein

Phosphatidylinositol 3-kinase catalytic subunit type 3

Gene

Pik3c3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 96 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    Catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate which plays a key role in initiation and maturation of autophagosomes. Involved in the transport of lysosomal enzyme precursors to lysosomes. Required for the abcission step in cytokinesis. Required for transport from early to late endosomes By similarity.By similarity

    Catalytic activityi

    ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 3-phosphate.

    Cofactori

    Manganese.By similarity

    GO - Molecular functioni

    1. 1-phosphatidylinositol-3-kinase activity Source: UniProtKB
    2. ATP binding Source: UniProtKB-KW
    3. protein binding Source: MGI
    4. protein kinase activity Source: MGI

    GO - Biological processi

    1. autophagic vacuole assembly Source: Ensembl
    2. cytokinesis Source: UniProtKB
    3. early endosome to late endosome transport Source: UniProtKB
    4. endosome organization Source: Ensembl
    5. phosphatidylinositol-mediated signaling Source: Ensembl
    6. protein phosphorylation Source: MGI
    7. protein processing Source: Ensembl
    8. regulation of protein secretion Source: Ensembl
    9. response to leucine Source: Ensembl

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Autophagy, Cell cycle, Cell division

    Keywords - Ligandi

    ATP-binding, Manganese, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_198970. Synthesis of PIPs at the Golgi membrane.
    REACT_198972. Synthesis of PIPs at the late endosome membrane.
    REACT_198975. Synthesis of PIPs at the early endosome membrane.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphatidylinositol 3-kinase catalytic subunit type 3 (EC:2.7.1.137)
    Short name:
    PI3-kinase type 3
    Short name:
    PI3K type 3
    Short name:
    PtdIns-3-kinase type 3
    Alternative name(s):
    Phosphoinositide-3-kinase class 3
    Gene namesi
    Name:Pik3c3
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 18

    Organism-specific databases

    MGIiMGI:2445019. Pik3c3.

    Subcellular locationi

    Midbody By similarity. Late endosome By similarity
    Note: Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme.1 Publication

    GO - Cellular componenti

    1. late endosome Source: UniProtKB
    2. midbody Source: UniProtKB
    3. phagocytic vesicle Source: MGI
    4. phosphatidylinositol 3-kinase complex Source: RefGenome

    Keywords - Cellular componenti

    Endosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 887887Phosphatidylinositol 3-kinase catalytic subunit type 3PRO_0000088803Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei261 – 2611PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ6PF93.
    PaxDbiQ6PF93.
    PRIDEiQ6PF93.

    PTM databases

    PhosphoSiteiQ6PF93.

    Expressioni

    Gene expression databases

    ArrayExpressiQ6PF93.
    BgeeiQ6PF93.
    CleanExiMM_PIK3C3.
    GenevestigatoriQ6PF93.

    Interactioni

    Subunit structurei

    Heterodimer. This subunit, part of a complex composed of regulatory and catalytic subunits, associates with regulatory subunit PIK3R4 By similarity. Interacts with AMBRA1. Forms a complex with BECN1, PIK3R4 and either UVRAG and KIAA0226/Rubicon, or with ATG14. In this complex, presence of UVRAG and ATG14 are mutually exclusive By similarity. Part of a complex composed of PIK3R4 and PIK3CB. Interacts with RAB7A in the presence of PIK3R4. Interacts with BECN1P1/BECN2 By similarity.By similarity

    Protein-protein interaction databases

    BioGridi230382. 2 interactions.
    DIPiDIP-57222N.
    IntActiQ6PF93. 1 interaction.
    MINTiMINT-4608698.
    STRINGi10090.ENSMUSP00000111479.

    Structurei

    3D structure databases

    ProteinModelPortaliQ6PF93.
    SMRiQ6PF93. Positions 57-147, 282-871.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini35 – 184150C2 PI3K-typePROSITE-ProRule annotationAdd
    BLAST
    Domaini283 – 520238PIK helicalPROSITE-ProRule annotationAdd
    BLAST
    Domaini631 – 885255PI3K/PI4KPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the PI3/PI4-kinase family.PROSITE-ProRule annotation
    Contains 1 C2 PI3K-type domain.PROSITE-ProRule annotation
    Contains 1 PI3K/PI4K domain.PROSITE-ProRule annotation
    Contains 1 PIK helical domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5032.
    GeneTreeiENSGT00620000087805.
    HOGENOMiHOG000174003.
    HOVERGENiHBG082145.
    InParanoidiQ6PF93.
    KOiK00914.
    OMAiYLMVEFP.
    OrthoDBiEOG7PP55Z.
    PhylomeDBiQ6PF93.
    TreeFamiTF102032.

    Family and domain databases

    Gene3Di1.10.1070.11. 1 hit.
    1.25.40.70. 2 hits.
    2.60.40.150. 1 hit.
    InterProiIPR016024. ARM-type_fold.
    IPR000008. C2_dom.
    IPR011009. Kinase-like_dom.
    IPR000403. PI3/4_kinase_cat_dom.
    IPR018936. PI3/4_kinase_CS.
    IPR002420. PI3K_C2_dom.
    IPR008290. PI3K_Vps34.
    IPR015433. PI_Kinase.
    IPR001263. PInositide-3_kin_accessory_dom.
    [Graphical view]
    PANTHERiPTHR10048. PTHR10048. 1 hit.
    PfamiPF00454. PI3_PI4_kinase. 1 hit.
    PF00792. PI3K_C2. 1 hit.
    PF00613. PI3Ka. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000587. PI3K_Vps34. 1 hit.
    SMARTiSM00239. C2. 1 hit.
    SM00142. PI3K_C2. 1 hit.
    SM00145. PI3Ka. 1 hit.
    SM00146. PI3Kc. 1 hit.
    [Graphical view]
    SUPFAMiSSF48371. SSF48371. 2 hits.
    SSF49562. SSF49562. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEiPS00915. PI3_4_KINASE_1. 1 hit.
    PS00916. PI3_4_KINASE_2. 1 hit.
    PS50290. PI3_4_KINASE_3. 1 hit.
    PS51547. PI3K_C2. 1 hit.
    PS51545. PIK_HELICAL. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q6PF93-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MGEAEKFHYI YSCDLDINVQ LKIGSLEGKR EQKSYKAVLE DPMLKFSGLY    50
    QETCSDLYVT CQVFAEGKPL ALPVRTSYKA FSTRWNWNEW LKLPVKYPDL 100
    PRNAQVALTI WDVYGPGSAV PVGGTTVSLF GKYGMFRQGM HDLKVWPNVE 150
    ADGSEPTRTP GRTSSTLSED QMSRLAKLTK AHRQGHMVKV DWLDRLTFRE 200
    IEMINESEKR SSNFMYLMVE FRCVKCDDKE YGIVYYEKDG DESSPILTSF 250
    ELVKVPDPQM SMENLVESKH HKLARSLRSG PSDHDLKPNA TTRDQLNIIV 300
    SYPPTKQLTY EEQDLVWKFR YYLTNQEKAL TKFLKCVNWD LPQEAKQALE 350
    LLGKWKPMDV EDSLELLSSH YTNPTVRRYA VARLRQADDE DLLMYLLQLV 400
    QALKYENFDD IKNGLEPTKK DSQTSASESL SNSGVSSGDI DSSQIITNPL 450
    PPVASPPPAS KAKEVSDGEN LEQDLCTFLI SRACKNSTLA NYLYWYVIVE 500
    CEDQDTQQRD PKTHEMYLNV MRRFSQALLK GDKSVRVMRS LLAAQQTFVD 550
    RLVHLMKAVQ RESGNRKKKN ERLQALLGDN EKMNLSDVEL IPLPLEPQVK 600
    IRGIIPETAT LFKSALMPAQ LFFKTEDGGK YPVIFKHGDD LRQDQLILQI 650
    ISLMDKLLRK ENLDLKLTPY KVLATSTKHG FMQFIQSVPV AEVLDTEGSI 700
    QNFFRKYAPS ETGPYGISAE VMDTYVKSCA GYCVITYILG VGDRHLDNLL 750
    LTKTGKLFHI DFGYILGRDP KPLPPPMKLN KEMVEGMGGT QSEQYQEFRK 800
    QCYTAFLHLR RYSNLILNLF SLMVDANIPD IALEPDKTVK KVQDKFRLDL 850
    SDEEAVHYMQ SLIDESVHAL FAAVVEQIHK FAQYWRK 887
    Length:887
    Mass (Da):101,487
    Last modified:July 5, 2004 - v1
    Checksum:i80E8EF9EF3AF74D7
    GO
    Isoform 2 (identifier: Q6PF93-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         842-848: VQDKFRL → ISCHRKM
         849-887: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:848
    Mass (Da):96,869
    Checksum:i26219164AC269CFB
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti455 – 4551Missing in AAH57678. (PubMed:15489334)Curated
    Sequence conflicti470 – 4701N → S in AAH57678. (PubMed:15489334)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei842 – 8487VQDKFRL → ISCHRKM in isoform 2. 1 PublicationVSP_016459
    Alternative sequencei849 – 88739Missing in isoform 2. 1 PublicationVSP_016460Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC024675 mRNA. Translation: AAH24675.1.
    BC057678 mRNA. Translation: AAH57678.1.
    CCDSiCCDS37752.1. [Q6PF93-1]
    RefSeqiNP_852079.2. NM_181414.5. [Q6PF93-1]
    XP_006525902.1. XM_006525839.1. [Q6PF93-1]
    UniGeneiMm.194127.

    Genome annotation databases

    EnsembliENSMUST00000115812; ENSMUSP00000111479; ENSMUSG00000033628. [Q6PF93-1]
    GeneIDi225326.
    KEGGimmu:225326.
    UCSCiuc008ehw.1. mouse. [Q6PF93-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC024675 mRNA. Translation: AAH24675.1 .
    BC057678 mRNA. Translation: AAH57678.1 .
    CCDSi CCDS37752.1. [Q6PF93-1 ]
    RefSeqi NP_852079.2. NM_181414.5. [Q6PF93-1 ]
    XP_006525902.1. XM_006525839.1. [Q6PF93-1 ]
    UniGenei Mm.194127.

    3D structure databases

    ProteinModelPortali Q6PF93.
    SMRi Q6PF93. Positions 57-147, 282-871.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 230382. 2 interactions.
    DIPi DIP-57222N.
    IntActi Q6PF93. 1 interaction.
    MINTi MINT-4608698.
    STRINGi 10090.ENSMUSP00000111479.

    PTM databases

    PhosphoSitei Q6PF93.

    Proteomic databases

    MaxQBi Q6PF93.
    PaxDbi Q6PF93.
    PRIDEi Q6PF93.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000115812 ; ENSMUSP00000111479 ; ENSMUSG00000033628 . [Q6PF93-1 ]
    GeneIDi 225326.
    KEGGi mmu:225326.
    UCSCi uc008ehw.1. mouse. [Q6PF93-1 ]

    Organism-specific databases

    CTDi 5289.
    MGIi MGI:2445019. Pik3c3.

    Phylogenomic databases

    eggNOGi COG5032.
    GeneTreei ENSGT00620000087805.
    HOGENOMi HOG000174003.
    HOVERGENi HBG082145.
    InParanoidi Q6PF93.
    KOi K00914.
    OMAi YLMVEFP.
    OrthoDBi EOG7PP55Z.
    PhylomeDBi Q6PF93.
    TreeFami TF102032.

    Enzyme and pathway databases

    Reactomei REACT_198970. Synthesis of PIPs at the Golgi membrane.
    REACT_198972. Synthesis of PIPs at the late endosome membrane.
    REACT_198975. Synthesis of PIPs at the early endosome membrane.

    Miscellaneous databases

    ChiTaRSi PIK3C3. mouse.
    NextBioi 377617.
    PROi Q6PF93.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q6PF93.
    Bgeei Q6PF93.
    CleanExi MM_PIK3C3.
    Genevestigatori Q6PF93.

    Family and domain databases

    Gene3Di 1.10.1070.11. 1 hit.
    1.25.40.70. 2 hits.
    2.60.40.150. 1 hit.
    InterProi IPR016024. ARM-type_fold.
    IPR000008. C2_dom.
    IPR011009. Kinase-like_dom.
    IPR000403. PI3/4_kinase_cat_dom.
    IPR018936. PI3/4_kinase_CS.
    IPR002420. PI3K_C2_dom.
    IPR008290. PI3K_Vps34.
    IPR015433. PI_Kinase.
    IPR001263. PInositide-3_kin_accessory_dom.
    [Graphical view ]
    PANTHERi PTHR10048. PTHR10048. 1 hit.
    Pfami PF00454. PI3_PI4_kinase. 1 hit.
    PF00792. PI3K_C2. 1 hit.
    PF00613. PI3Ka. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000587. PI3K_Vps34. 1 hit.
    SMARTi SM00239. C2. 1 hit.
    SM00142. PI3K_C2. 1 hit.
    SM00145. PI3Ka. 1 hit.
    SM00146. PI3Kc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48371. SSF48371. 2 hits.
    SSF49562. SSF49562. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEi PS00915. PI3_4_KINASE_1. 1 hit.
    PS00916. PI3_4_KINASE_2. 1 hit.
    PS50290. PI3_4_KINASE_3. 1 hit.
    PS51547. PI3K_C2. 1 hit.
    PS51545. PIK_HELICAL. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Tissue: Mammary gland.
    2. Cited for: INTERACTION WITH BECN1 AND AMBRA1.
    3. "The class IA phosphatidylinositol 3-kinase p110-beta subunit is a positive regulator of autophagy."
      Dou Z., Chattopadhyay M., Pan J.-A., Guerriero J.L., Jiang Y.-P., Ballou L.M., Yue Z., Lin R.Z., Zong W.-X.
      J. Cell Biol. 191:827-843(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN A COMPLEX WITH PIK3R4 AND PIK3CB.
    4. Cited for: SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiPK3C3_MOUSE
    AccessioniPrimary (citable) accession number: Q6PF93
    Secondary accession number(s): Q8R3S8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 6, 2005
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 96 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3