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Protein

Runt-related transcription factor 1

Gene

runx1

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in primitive hematopoiesis in the embryo.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei112Chloride 1PROSITE-ProRule annotationBy similarity1
Binding sitei116Chloride 1; via amide nitrogenPROSITE-ProRule annotationBy similarity1
Binding sitei139Chloride 2PROSITE-ProRule annotationBy similarity1
Binding sitei170Chloride 2; via amide nitrogenPROSITE-ProRule annotationBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein, DNA-binding
Biological processTranscription, Transcription regulation
LigandChloride

Names & Taxonomyi

Protein namesi
Recommended name:
Runt-related transcription factor 1By similarity
Alternative name(s):
Acute myeloid leukemia 1 protein1 Publication
Short name:
XAMLImported
Core-binding factor subunit alpha-21 Publication
Short name:
CBF-alpha-22 Publications
Gene namesi
Name:runx1By similarity
Synonyms:amlImported, cbfa21 Publication
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-864949. runx1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003512071 – 462Runt-related transcription factor 1Add BLAST462

Expressioni

Tissue specificityi

Shows a complex and dynamic expression pattern. In stage 14-24 embryos, expressed in a subset of neuroblasts in the lateral stripe of the neural plate. In late neurula stages, expression begins in the olfactory placodes. Also expressed in structures that play a role in blood formation: at stage 14, expressed on the anterior ventral side of the embryo in the anterior endomesoderm. As the embryo elongates, expression shifts gradually to a V-shaped expression pattern in the presumptive ventral blood island.1 Publication

Developmental stagei

Expressed both maternally and zygotically. Shows a small amount of maternal expression. Zygotic expression begins at stage 8 and steadily increases after this point.1 Publication

Inductioni

By low levels of the dorsalizing factor LiCl. Inhibited by high doses of LiCl. In order to temporally control hematopoiesis, fgf-signaling inhibits expression in posterior regions of the embryo by antagonizing bmp-signaling.2 Publications

Interactioni

Subunit structurei

Heterodimer with cbfb. runx1 binds DNA as a monomer and through the Runt domain. DNA-binding is increased by heterodimerization (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

ELMiQ6PF39.

Structurei

3D structure databases

SMRiQ6PF39.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini50 – 178RuntPROSITE-ProRule annotationAdd BLAST129

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni80 – 84Interaction with DNABy similarity5
Regioni135 – 143Interaction with DNABy similarity9
Regioni168 – 177Interaction with DNABy similarity10

Phylogenomic databases

HOVERGENiHBG060268.
KOiK08367.

Family and domain databases

Gene3Di2.60.40.720. 1 hit.
4.10.770.10. 1 hit.
InterProiView protein in InterPro
IPR000040. AML1_Runt.
IPR008967. p53-like_TF_DNA-bd.
IPR012346. p53/RUNT-type_TF_DNA-bd_sf.
IPR013524. Runt_dom.
IPR027384. Runx_central_dom_sf.
IPR013711. RunxI_C_dom.
IPR016554. TF_Runt-rel_RUNX.
PANTHERiPTHR11950. PTHR11950. 1 hit.
PfamiView protein in Pfam
PF00853. Runt. 1 hit.
PF08504. RunxI. 1 hit.
PIRSFiPIRSF009374. TF_Runt-rel_RUNX. 1 hit.
PRINTSiPR00967. ONCOGENEAML1.
SUPFAMiSSF49417. SSF49417. 1 hit.
PROSITEiView protein in PROSITE
PS51062. RUNT. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 11 Publication (identifier: Q6PF39-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRIPVDTSTS RRFTPPSTTL SPGKMSEPIP LNIADSSAAL VGKLRSTDRN
60 70 80 90 100
MVEVLSDHPG ELVRTDSPNF LCSVLPTHWR CNKTLPIAFK VVALGEVPDG
110 120 130 140 150
TLVTVMAGND ENYSAELRNA TAAMKSQVAR FNDLRFVGRS GRGKSFTLTI
160 170 180 190 200
TVFTNPPQVA TYHRAIKITV DGPREPRRHR QKLDEQTKPG NLSFSERLSE
210 220 230 240 250
LEHFRRTAMR VSPHHPNPMP NPRATLNHSA AFNPQPQGQI QVADTRQVQA
260 270 280 290 300
SPPWSYDQSY QYLGSIATQS VHPATPISPG RASSMTSLSA ELSSRLSGAS
310 320 330 340 350
DLTAFSDPRV GIDRQFSTLP SISDPRMHYP GAFTYTPTPV TSGIGIGMSA
360 370 380 390 400
MTSATRYHTY LPPPYPGSSQ AQSNPFQTSS PSYHLYYGTS AGSYHQFSMM
410 420 430 440 450
SGGERSPPRI LPPCTNASTG STLLNPNLPN QSDVVEAEGS HSNSPTNMGS
460
TPRLEEAVWR PY
Length:462
Mass (Da):50,369
Last modified:September 23, 2008 - v2
Checksum:iE6A38D9B8ED07B3A
GO
Isoform 2 (identifier: Q6PF39-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-5: MRIPV → MASHSAFQSFPLYPPCFFR

Note: No experimental confirmation available.Curated
Show »
Length:476
Mass (Da):51,988
Checksum:i2ED277BEE99A9ADC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti74V → L in AAC41269 (PubMed:9502719).Curated1
Sequence conflicti195S → P in AAC41269 (PubMed:9502719).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0529471 – 5MRIPV → MASHSAFQSFPLYPPCFFR in isoform 2. 1 Publication5

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF035446 mRNA. Translation: AAC41269.1.
BC057739 mRNA. Translation: AAH57739.1.
RefSeqiNP_001079966.1. NM_001086497.1. [Q6PF39-2]
XP_018100278.1. XM_018244789.1. [Q6PF39-2]
UniGeneiXl.2874.

Genome annotation databases

GeneIDi379657.
KEGGixla:379657.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiRUNX1_XENLA
AccessioniPrimary (citable) accession number: Q6PF39
Secondary accession number(s): O73725
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 23, 2008
Last sequence update: September 23, 2008
Last modified: November 22, 2017
This is version 71 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi