Reviewed, UniProtKB/Swiss-Prot Q6PF21 (SYMC_XENLA)

Names and origin

Protein names

Recommended name:
    Methionyl-tRNA synthetase, cytoplasmic
    EC=6.1.1.10
Alternative name(s):
    Methionine--tRNA ligase
      Short name=MetRS

Gene names

Name:

mars

Organism

Xenopus laevis (African clawed frog)

Taxonomic identifier

8355 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Mesobatrachia › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length	905 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met).
Subunit structure	Component of the multisynthetase complex which is comprised of a bifunctional glutamyl-prolyl-tRNA synthetase, the monospecific isoleucyl, leucyl, glutaminyl, methionyl, lysyl, arginyl, and aspartyl-tRNA synthetases as well as three auxiliary proteins By similarity.
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain. Contains 1 WHEP-TRS domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding RNA-binding tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Gene Ontology (GO)
Biological process	methionyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW methionine-tRNA ligase activity Inferred from electronic annotation. Source: EC tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 905	905	Methionyl-tRNA synthetase, cytoplasmic		PRO_0000139264
Regions
	Domain	1 – 75	75	GST N-terminal
	Domain	72 – 199	128	GST C-terminal
	Domain	844 – 900	57	WHEP-TRS
	Motif	271 – 281	11	"HIGH" region
	Motif	591 – 595	5	"KMSKS" region
Sites
	Binding site	594	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6PF21-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: C79BFA3254468964	FASTA	905	102,359
	10 20 30 40 50 60 MKLFVGEGNP QGVKVLAAAA LWAQHVQIDR LQQEEKIVPF MSQPRLPVLD LENGNYLFLS 70 80 90 100 110 120 NAICRYFYLS SGHDMCDLSN QWLEWEAAEL QPALSAALYA HVVQGKKKED VMATISASLK 130 140 150 160 170 180 HLDQSLAGKS SPYLIKDALT VVDIVVWGSI YPLIVDASNL PEEMASLKRW FQNVSQLEQC 190 200 210 220 230 240 QKAASSLLKD KGSSVFKPFL QKQPAPITPP GKSVCKEQEG EDMPSLSEED IQAAAEAWAK 250 260 270 280 290 300 GLTGASKPKQ RPHPILPVEG EKNVLITSAL PYVNNVPHLG NIIGSVLSAD VFARYCRLRN 310 320 330 340 350 360 WNTLYICGTD EYGTATETKA MEEGLTPQQI CDKYNAIHTA IYQWFNISFD YFGRTTTQHQ 370 380 390 400 410 420 TTISQDIFHR LLEREFLLTD TVEQLRCEKC QRFLADRFVE GICPFCNYEE ARGDQCDKCG 430 440 450 460 470 480 KLINAVELKK PQCKICKQSP VIKSSKHLFL DLPKLEKRLE QWLEQSFSTG DWTSNARFIT 490 500 510 520 530 540 RSWIRDGLKP RCITRDLKWG TPVPLDGFRD KVFYVWFDAP IGYISITANY TDQWEKWWKS 550 560 570 580 590 600 PQQVQLYNFM AKDNVPFHSV VFPSCLLGAE DNYTLVNHLV ATEYLNYEDG KFSKSRGVGV 610 620 630 640 650 660 FGDMAKDTGI PADIWRFYLL YVRPEGQDSA FSWSDLMLKN NSELLNNLGN FVNRAGMFVQ 670 680 690 700 710 720 KFFNGCVPEM ELLSEDKRLL AQVAAELQQY NLLLEKVRIR DALRCILNIS RHGNQYIQVN 730 740 750 760 770 780 EPWKCIKGNQ QEQKRAGTVT GVAVNMAALL SIMLHPYMPT ISSVIQEQLL MPQESKVLTT 790 800 810 820 830 840 DFCCCLQSGH QIGNVSPLFQ KLENDQIESL RKRFGGGQVK TESKVSPSQE APEQQAPKAS 850 860 870 880 890 900 GPERVKELMQ ELEKQGNHVR ELKGKKAEKS VIDPEVQKLL ALKKELALAE GKSPDPPTQK GKKKK

References

[1]	NIH - Xenopus Gene Collection (XGC) project Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Ovary.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC057757 mRNA. Translation: AAH57757.1.
RefSeq	NP_001079984.1.
UniGene	Xl.25606
3D structure databases
SMR	Q6PF21. Positions 1-199, 224-770, 262-812, 836-903.
ModBase	Search...
Genome annotation databases
GeneID	379675.
KEGG	xla:379675.
Phylogenomic databases
HOVERGEN	Q6PF21.
Enzyme and pathway databases
BRENDA	6.1.1.10. 648.
Family and domain databases
InterPro	IPR015413. aa-tRNA-synt_I. IPR001412. aa-tRNA-synth_I_CS. IPR010987. Glutathione-S-Trfase_C-like. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR002304. Met-tRNA-synth_Ia. IPR014729. Rossmann-like_a/b/a_fold. IPR009068. S15_NS1_RNA_bd. IPR012336. Thioredoxin-like_fold. IPR014758. tRNA-synt_met_N. IPR009080. tRNAsynth_1a_anticodon-bd. IPR013155. V/L/I-tRNA-synth_anticodon-bd. IPR000738. WHEP-TRS. [Graphical view]
Gene3D	G3DSA:1.20.1050.10. GST_C_like. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit. G3DSA:1.10.287.10. S15_NS1_RNA_bd. 1 hit.
Pfam	PF08264. Anticodon_1. 1 hit. PF09334. tRNA-synt_1g. 1 hit. PF00458. WHEP-TRS. 1 hit. [Graphical view]
PRINTS	PR01041. TRNASYNTHMET.
TIGRFAMs	TIGR00398. metG. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. PS50405. GST_CTER. 1 hit. PS50404. GST_NTER. 1 hit. PS00762. WHEP_TRS_1. 1 hit. PS51185. WHEP_TRS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYMC_XENLA

Accession

Primary (citable) accession number: Q6PF21

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2005
Last sequence update:	July 5, 2004
Last modified:	February 9, 2010
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Xenopus annotation project