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Protein

Microtubule-associated protein RP/EB family member 3

Gene

Mapre3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds to the plus end of microtubules and regulates the dynamics of the microtubule cytoskeleton. Promotes microtubule growth. May be involved in spindle function by stabilizing microtubules and anchoring them at centrosomes. May play a role in cell migration (By similarity).By similarity

GO - Molecular functioni

  1. microtubule binding Source: BHF-UCL

GO - Biological processi

  1. cell division Source: UniProtKB-KW
  2. mitotic nuclear division Source: UniProtKB-KW
  3. positive regulation of cyclin-dependent protein serine/threonine kinase activity Source: BHF-UCL
  4. positive regulation of microtubule plus-end binding Source: MGI
  5. positive regulation of transcription, DNA-templated Source: BHF-UCL
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Names & Taxonomyi

Protein namesi
Recommended name:
Microtubule-associated protein RP/EB family member 3
Alternative name(s):
EB1 protein family member 3
Short name:
EBF3
End-binding protein 3
Short name:
EB3
RP3
Gene namesi
Name:Mapre3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 5

Organism-specific databases

MGIiMGI:2140967. Mapre3.

Subcellular locationi

Cytoplasm By similarity. Cytoplasmcytoskeleton By similarity
Note: Associated with the microtubule network. Detected at the plus end of microtubules (By similarity).By similarity

GO - Cellular componenti

  1. cytoplasm Source: BHF-UCL
  2. cytoplasmic microtubule Source: Ensembl
  3. microtubule cytoskeleton Source: BHF-UCL
  4. midbody Source: BHF-UCL
  5. perinuclear region of cytoplasm Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 281280Microtubule-associated protein RP/EB family member 3PRO_0000213429Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei124 – 1241PhosphotyrosineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ6PER3.
PaxDbiQ6PER3.
PRIDEiQ6PER3.

PTM databases

PhosphoSiteiQ6PER3.

Expressioni

Gene expression databases

BgeeiQ6PER3.
ExpressionAtlasiQ6PER3. baseline and differential.
GenevestigatoriQ6PER3.

Interactioni

Subunit structurei

Homodimer. Heterodimer with MAPRE1. Interacts with DCTN1 and SRCIN1. Binds to the C-terminal domain of APC. Binds monomeric and polymerized tubulin. Interacts (via C-terminus) with CLIP1. Interacts with SLAIN2 (By similarity). Interacts with APC2.By similarity1 Publication

Protein-protein interaction databases

IntActiQ6PER3. 1 interaction.
MINTiMINT-4101277.

Structurei

3D structure databases

ProteinModelPortaliQ6PER3.
SMRiQ6PER3. Positions 1-147, 204-260.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini14 – 116103CHPROSITE-ProRule annotationAdd
BLAST
Domaini194 – 26471EB1 C-terminalPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni217 – 28165DCTN1-bindingBy similarityAdd
BLAST
Regioni217 – 26044APC-bindingBy similarityAdd
BLAST

Domaini

Composed of two functionally independent domains. The N-terminal domain forms a hydrophobic cleft involved in microtubule binding and the C-terminal is involved in the formation of mutually exclusive complexes with APC and DCTN1 (By similarity).By similarity

Sequence similaritiesi

Belongs to the MAPRE family.Curated
Contains 1 CH (calponin-homology) domain.PROSITE-ProRule annotation
Contains 1 EB1 C-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5217.
GeneTreeiENSGT00490000043329.
HOGENOMiHOG000198048.
HOVERGENiHBG052410.
InParanoidiQ6PER3.
KOiK10436.
OMAiILSWIND.
OrthoDBiEOG7ZD1W5.
PhylomeDBiQ6PER3.
TreeFamiTF313620.

Family and domain databases

Gene3Di1.10.418.10. 1 hit.
InterProiIPR001715. CH-domain.
IPR004953. EB1_C.
IPR027738. EB3_vertebrate.
IPR027328. MAPRE.
[Graphical view]
PANTHERiPTHR10623. PTHR10623. 1 hit.
PTHR10623:SF10. PTHR10623:SF10. 1 hit.
PfamiPF00307. CH. 1 hit.
PF03271. EB1. 1 hit.
[Graphical view]
SUPFAMiSSF140612. SSF140612. 1 hit.
SSF47576. SSF47576. 1 hit.
PROSITEiPS50021. CH. 1 hit.
PS51230. EB1_C. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6PER3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVNVYSTSV TSENLSRHDM LAWVNDSLHL NYTKIEQLCS GAAYCQFMDM
60 70 80 90 100
LFPGCVHLRK VKFQAKLEHE YIHNFKVLQA AFKKMGVDKI IPVEKLVKGK
110 120 130 140 150
FQDNFEFIQW FKKFFDANYD GKDYNPLLAR QGQDVAPPPN PGDQIFNKSK
160 170 180 190 200
KLIGTAVPQR TSPTGPKNMQ TSGRLSNVAP PCILRKNPPS ARNGGHEADA
210 220 230 240 250
QILELNQQLL DLKLTVDGLE KERDFYFSKL RDIELICQEH ESENSPVISG
260 270 280
IIGILYATEE GFAPPEDDEI EEHQQEDQDE Y
Length:281
Mass (Da):31,966
Last modified:July 5, 2004 - v1
Checksum:i6713427C480838DC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC057918 mRNA. Translation: AAH57918.1.
U51204 mRNA. Translation: AAA96321.1.
CCDSiCCDS19163.1.
RefSeqiNP_579928.1. NM_133350.1.
XP_006503686.1. XM_006503623.1.
UniGeneiMm.332295.
Mm.471154.

Genome annotation databases

EnsembliENSMUST00000031058; ENSMUSP00000031058; ENSMUSG00000029166.
GeneIDi100732.
KEGGimmu:100732.
UCSCiuc008wvz.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC057918 mRNA. Translation: AAH57918.1.
U51204 mRNA. Translation: AAA96321.1.
CCDSiCCDS19163.1.
RefSeqiNP_579928.1. NM_133350.1.
XP_006503686.1. XM_006503623.1.
UniGeneiMm.332295.
Mm.471154.

3D structure databases

ProteinModelPortaliQ6PER3.
SMRiQ6PER3. Positions 1-147, 204-260.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ6PER3. 1 interaction.
MINTiMINT-4101277.

PTM databases

PhosphoSiteiQ6PER3.

Proteomic databases

MaxQBiQ6PER3.
PaxDbiQ6PER3.
PRIDEiQ6PER3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000031058; ENSMUSP00000031058; ENSMUSG00000029166.
GeneIDi100732.
KEGGimmu:100732.
UCSCiuc008wvz.1. mouse.

Organism-specific databases

CTDi22924.
MGIiMGI:2140967. Mapre3.

Phylogenomic databases

eggNOGiCOG5217.
GeneTreeiENSGT00490000043329.
HOGENOMiHOG000198048.
HOVERGENiHBG052410.
InParanoidiQ6PER3.
KOiK10436.
OMAiILSWIND.
OrthoDBiEOG7ZD1W5.
PhylomeDBiQ6PER3.
TreeFamiTF313620.

Miscellaneous databases

ChiTaRSiMapre3. mouse.
NextBioi354616.
PROiQ6PER3.
SOURCEiSearch...

Gene expression databases

BgeeiQ6PER3.
ExpressionAtlasiQ6PER3. baseline and differential.
GenevestigatoriQ6PER3.

Family and domain databases

Gene3Di1.10.418.10. 1 hit.
InterProiIPR001715. CH-domain.
IPR004953. EB1_C.
IPR027738. EB3_vertebrate.
IPR027328. MAPRE.
[Graphical view]
PANTHERiPTHR10623. PTHR10623. 1 hit.
PTHR10623:SF10. PTHR10623:SF10. 1 hit.
PfamiPF00307. CH. 1 hit.
PF03271. EB1. 1 hit.
[Graphical view]
SUPFAMiSSF140612. SSF140612. 1 hit.
SSF47576. SSF47576. 1 hit.
PROSITEiPS50021. CH. 1 hit.
PS51230. EB1_C. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Liver.
  2. Hoffman N.G., Kay B.K.
    Submitted (MAR-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 57-281.
  3. "p53 downstream target DDA3 is a novel microtubule-associated protein that interacts with end-binding protein EB3 and activates beta-catenin pathway."
    Hsieh P.-C., Chang J.-C., Sun W.-T., Hsieh S.-C., Wang M.-C., Wang F.-F.
    Oncogene 26:4928-4940(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH APC2.

Entry informationi

Entry nameiMARE3_MOUSE
AccessioniPrimary (citable) accession number: Q6PER3
Secondary accession number(s): Q61167
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: July 5, 2004
Last modified: March 4, 2015
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.