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Q6PEM8 (PCFT_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Proton-coupled folate transporter
Alternative name(s):
Heme carrier protein 1
PCFT/HCP1
Solute carrier family 46 member 1
Gene names
Name:Slc46a1
Synonyms:D11Ertd18e, Hcp1, Pcft
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length459 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Has been shown to act both as an intestinal proton-coupled high-affinity folate transporter and as an intestinal heme transporter which mediates heme uptake from the gut lumen into duodenal epithelial cells. The iron is then released from heme and may be transported into the bloodstream. Dietary heme iron is an important nutritional source of iron. Shows a higher affinity for folate than heme. Ref.5

Subunit structure

Monomer By similarity.

Subcellular location

Apical cell membrane; Multi-pass membrane protein. Cytoplasm. Note: Localizes to the apical membrane of intestinal cells in iron-deficient cells, while it resides in internal cellular compartments in iron-replete cells. Ref.5

Tissue specificity

Highly expressed in duodenum, especially in duodenal mucosa, the main site of intestinal heme absorption. Weakly expressed in the kidney. Not expressed in duodenum before weaning or in placenta. Weakly or not expressed in brain, heart, lung, skeletal muscle, testis and neonatal liver. Ref.5

Induction

Up-regulated in response to hypoxia, it is however unclear whether such up-regulation is direct or not. Not induced in the duodenum of iron-deficient mice. Ref.5

Sequence similarities

Belongs to the major facilitator superfamily. SLC46A family.

Sequence caution

The sequence BAD90126.1 differs from that shown. Reason: Erroneous initiation.

The sequence CAI25543.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 459459Proton-coupled folate transporter
PRO_0000084852

Regions

Transmembrane28 – 4821Helical; Potential
Transmembrane87 – 10721Helical; Potential
Transmembrane115 – 13521Helical; Potential
Transmembrane149 – 16921Helical; Potential
Transmembrane183 – 20321Helical; Potential
Transmembrane211 – 23121Helical; Potential
Transmembrane267 – 28721Helical; Potential
Transmembrane303 – 32523Helical; Potential
Transmembrane337 – 35721Helical; Potential
Transmembrane390 – 41021Helical; Potential
Transmembrane423 – 44321Helical; Potential

Amino acid modifications

Modified residue11N-acetylmethionine By similarity
Modified residue4581Phosphoserine Ref.6
Glycosylation581N-linked (GlcNAc...) Ref.7

Experimental info

Sequence conflict4021A → V in BAB22685. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q6PEM8 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: A251124B3B9846AF

FASTA45950,089
        10         20         30         40         50         60 
MEGRVSSVGS PHSFLNAPVL FRGPVEPLVF LANFALVLQG PLTTQYLWHR FSTELGYNGT 

        70         80         90        100        110        120 
RHRENCGNQS ADPLMKEVET LTSHWTLYMN VGGFLVGLFW STLLGAWSDR VGRRPLLVLA 

       130        140        150        160        170        180 
SLGLLLQAVV SIFVVQLELH VGFFVLGRAL CALLGDFNGL LAASFASVAD VSSNHSRTFR 

       190        200        210        220        230        240 
MALLEACIGV AGTLASLLGG HWLRAQGYAN PFWLALALLI VMALYAAFCF GETVKEPKST 

       250        260        270        280        290        300 
RLFTLRHHRS IARLYVVPAP EKSRMHLALY SLAIFVVVTV HFGAQDILTL YELSAPLCWD 

       310        320        330        340        350        360 
SKLIGYGSAA QHLPYLTSLL GLRLLQFCLA DTWVAEIGLA FNILGMVVFA FATITPLMFT 

       370        380        390        400        410        420 
GYGLLFLSLV TTPVIRAKLS KLVSESEQGA LFSAVACVNS LAMLMASGIF NSIYPATLNF 

       430        440        450 
MKGFPFLLGA GLLFIPAILI GVLEKVNPHP EFQQFPQSP 

« Hide

References

« Hide 'large scale' references
[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J and NOD.
[2]Okazaki N., Kikuno F.R., Nagase T., Ohara O., Koga H.
Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Spleen.
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Colon and Liver.
[5]"Identification of an intestinal heme transporter."
Shayeghi M., Latunde-Dada G.O., Oakhill J.S., Laftah A.H., Takeuchi K., Halliday N., Khan Y., Warley A., McCann F.E., Hider R.C., Frazer D.M., Anderson G.J., Vulpe C.D., Simpson R.J., McKie A.T.
Cell 122:789-801(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION.
[6]"The phagosomal proteome in interferon-gamma-activated macrophages."
Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-458, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[7]"Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-58.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK003278 mRNA. Translation: BAB22685.1.
AK170505 mRNA. Translation: BAE41843.1.
AK220201 mRNA. Translation: BAD90126.1. Different initiation.
AL591177 Genomic DNA. Translation: CAI25542.1.
AL591177 Genomic DNA. Translation: CAI25543.1. Sequence problems.
BC024522 mRNA. Translation: AAH24522.1.
BC057976 mRNA. Translation: AAH57976.1.
CCDSCCDS25104.1.
RefSeqNP_081016.2. NM_026740.2.
UniGeneMm.131618.

3D structure databases

ProteinModelPortalQ6PEM8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000001126.

PTM databases

PhosphoSiteQ6PEM8.

Proteomic databases

PRIDEQ6PEM8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000001126; ENSMUSP00000001126; ENSMUSG00000020829.
GeneID52466.
KEGGmmu:52466.
UCSCuc007kjg.2. mouse.

Organism-specific databases

CTD113235.
MGIMGI:1098733. Slc46a1.

Phylogenomic databases

eggNOGNOG306680.
GeneTreeENSGT00530000063076.
HOGENOMHOG000054191.
HOVERGENHBG055334.
InParanoidQ6PEM8.
KOK14613.
OMAQRGGCSN.
OrthoDBEOG7DNNVC.
PhylomeDBQ6PEM8.
TreeFamTF315701.

Gene expression databases

ArrayExpressQ6PEM8.
BgeeQ6PEM8.
CleanExMM_SLC46A1.
GenevestigatorQ6PEM8.

Family and domain databases

InterProIPR011701. MFS.
IPR016196. MFS_dom_general_subst_transpt.
IPR005829. Sugar_transporter_CS.
[Graphical view]
PfamPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMSSF103473. SSF103473. 1 hit.
ProtoNetSearch...

Other

ChiTaRSSLC46A1. mouse.
NextBio308995.
PROQ6PEM8.
SOURCESearch...

Entry information

Entry namePCFT_MOUSE
AccessionPrimary (citable) accession number: Q6PEM8
Secondary accession number(s): Q571I8 expand/collapse secondary AC list , Q5SYG0, Q8R1H7, Q9D1P1
Entry history
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: July 5, 2004
Last modified: July 9, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot