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Protein

Proton-coupled folate transporter

Gene

Slc46a1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Has been shown to act both as an intestinal proton-coupled high-affinity folate transporter and as an intestinal heme transporter which mediates heme uptake from the gut lumen into duodenal epithelial cells. The iron is then released from heme and may be transported into the bloodstream. Dietary heme iron is an important nutritional source of iron. Shows a higher affinity for folate than heme.1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processTransport
LigandFolate-binding

Enzyme and pathway databases

ReactomeiR-MMU-196757 Metabolism of folate and pterines
R-MMU-917937 Iron uptake and transport

Names & Taxonomyi

Protein namesi
Recommended name:
Proton-coupled folate transporter
Alternative name(s):
Heme carrier protein 1
PCFT/HCP1
Solute carrier family 46 member 1
Gene namesi
Name:Slc46a1
Synonyms:D11Ertd18e, Hcp1, Pcft
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1098733 Slc46a1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 24CytoplasmicSequence analysisAdd BLAST24
Transmembranei25 – 48HelicalSequence analysisAdd BLAST24
Topological domaini49 – 84ExtracellularSequence analysisAdd BLAST36
Transmembranei85 – 107HelicalSequence analysisAdd BLAST23
Topological domaini108 – 113CytoplasmicSequence analysis6
Transmembranei114 – 137HelicalSequence analysisAdd BLAST24
Topological domaini138 – 145ExtracellularSequence analysis8
Transmembranei146 – 168HelicalSequence analysisAdd BLAST23
Topological domaini169 – 180CytoplasmicSequence analysisAdd BLAST12
Transmembranei181 – 203HelicalSequence analysisAdd BLAST23
Topological domaini204 – 212ExtracellularSequence analysis9
Transmembranei213 – 236HelicalSequence analysisAdd BLAST24
Topological domaini237 – 265CytoplasmicSequence analysisAdd BLAST29
Transmembranei266 – 288HelicalSequence analysisAdd BLAST23
Topological domaini289 – 309ExtracellularSequence analysisAdd BLAST21
Transmembranei310 – 328HelicalSequence analysisAdd BLAST19
Topological domaini329 – 331CytoplasmicSequence analysis3
Transmembranei332 – 356HelicalSequence analysisAdd BLAST25
Topological domaini357 – 359ExtracellularSequence analysis3
Transmembranei360 – 381HelicalSequence analysisAdd BLAST22
Topological domaini382 – 393CytoplasmicSequence analysisAdd BLAST12
Transmembranei394 – 412HelicalSequence analysisAdd BLAST19
Topological domaini413 – 424ExtracellularSequence analysisAdd BLAST12
Transmembranei425 – 449HelicalSequence analysisAdd BLAST25
Topological domaini450 – 459CytoplasmicSequence analysis10

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000848521 – 459Proton-coupled folate transporterAdd BLAST459

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei6PhosphoserineBy similarity1
Glycosylationi58N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi68N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei458PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ6PEM8
PaxDbiQ6PEM8
PRIDEiQ6PEM8

PTM databases

iPTMnetiQ6PEM8
PhosphoSitePlusiQ6PEM8

Expressioni

Tissue specificityi

Highly expressed in duodenum, especially in duodenal mucosa, the main site of intestinal heme absorption. Weakly expressed in the kidney. Not expressed in duodenum before weaning or in placenta. Weakly or not expressed in brain, heart, lung, skeletal muscle, testis and neonatal liver.1 Publication

Inductioni

Up-regulated in response to hypoxia, it is however unclear whether such up-regulation is direct or not. Not induced in the duodenum of iron-deficient mice.1 Publication

Gene expression databases

BgeeiENSMUSG00000020829
CleanExiMM_SLC46A1
ExpressionAtlasiQ6PEM8 baseline and differential
GenevisibleiQ6PEM8 MM

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000001126

Structurei

3D structure databases

ProteinModelPortaliQ6PEM8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2816 Eukaryota
ENOG410ZVCA LUCA
GeneTreeiENSGT00530000063076
HOGENOMiHOG000054191
HOVERGENiHBG055334
InParanoidiQ6PEM8
KOiK14613
OMAiVQLYVTP
OrthoDBiEOG091G08MP
PhylomeDBiQ6PEM8
TreeFamiTF315701

Family and domain databases

CDDicd06174 MFS, 1 hit
InterProiView protein in InterPro
IPR011701 MFS
IPR020846 MFS_dom
IPR036259 MFS_trans_sf
IPR005829 Sugar_transporter_CS
PfamiView protein in Pfam
PF07690 MFS_1, 1 hit
SUPFAMiSSF103473 SSF103473, 1 hit

Sequencei

Sequence statusi: Complete.

Q6PEM8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEGRVSSVGS PHSFLNAPVL FRGPVEPLVF LANFALVLQG PLTTQYLWHR
60 70 80 90 100
FSTELGYNGT RHRENCGNQS ADPLMKEVET LTSHWTLYMN VGGFLVGLFW
110 120 130 140 150
STLLGAWSDR VGRRPLLVLA SLGLLLQAVV SIFVVQLELH VGFFVLGRAL
160 170 180 190 200
CALLGDFNGL LAASFASVAD VSSNHSRTFR MALLEACIGV AGTLASLLGG
210 220 230 240 250
HWLRAQGYAN PFWLALALLI VMALYAAFCF GETVKEPKST RLFTLRHHRS
260 270 280 290 300
IARLYVVPAP EKSRMHLALY SLAIFVVVTV HFGAQDILTL YELSAPLCWD
310 320 330 340 350
SKLIGYGSAA QHLPYLTSLL GLRLLQFCLA DTWVAEIGLA FNILGMVVFA
360 370 380 390 400
FATITPLMFT GYGLLFLSLV TTPVIRAKLS KLVSESEQGA LFSAVACVNS
410 420 430 440 450
LAMLMASGIF NSIYPATLNF MKGFPFLLGA GLLFIPAILI GVLEKVNPHP

EFQQFPQSP
Length:459
Mass (Da):50,089
Last modified:July 5, 2004 - v1
Checksum:iA251124B3B9846AF
GO

Sequence cautioni

The sequence BAD90126 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAI25543 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti402A → V in BAB22685 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK003278 mRNA Translation: BAB22685.1
AK170505 mRNA Translation: BAE41843.1
AK220201 mRNA Translation: BAD90126.1 Different initiation.
AL591177 Genomic DNA Translation: CAI25542.1
AL591177 Genomic DNA Translation: CAI25543.1 Sequence problems.
BC024522 mRNA Translation: AAH24522.1
BC057976 mRNA Translation: AAH57976.1
CCDSiCCDS25104.1
RefSeqiNP_081016.2, NM_026740.2
UniGeneiMm.131618

Genome annotation databases

EnsembliENSMUST00000001126; ENSMUSP00000001126; ENSMUSG00000020829
GeneIDi52466
KEGGimmu:52466
UCSCiuc007kjg.2 mouse

Similar proteinsi

Entry informationi

Entry nameiPCFT_MOUSE
AccessioniPrimary (citable) accession number: Q6PEM8
Secondary accession number(s): Q571I8
, Q5SYG0, Q8R1H7, Q9D1P1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: July 5, 2004
Last modified: May 23, 2018
This is version 122 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health