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Protein

Proton-coupled folate transporter

Gene

Slc46a1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Has been shown to act both as an intestinal proton-coupled high-affinity folate transporter and as an intestinal heme transporter which mediates heme uptake from the gut lumen into duodenal epithelial cells. The iron is then released from heme and may be transported into the bloodstream. Dietary heme iron is an important nutritional source of iron. Shows a higher affinity for folate than heme.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transport

Keywords - Ligandi

Folate-binding

Enzyme and pathway databases

ReactomeiR-MMU-196757. Metabolism of folate and pterines.
R-MMU-917937. Iron uptake and transport.

Names & Taxonomyi

Protein namesi
Recommended name:
Proton-coupled folate transporter
Alternative name(s):
Heme carrier protein 1
PCFT/HCP1
Solute carrier family 46 member 1
Gene namesi
Name:Slc46a1
Synonyms:D11Ertd18e, Hcp1, Pcft
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1098733. Slc46a1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 24CytoplasmicSequence analysisAdd BLAST24
Transmembranei25 – 48HelicalSequence analysisAdd BLAST24
Topological domaini49 – 84ExtracellularSequence analysisAdd BLAST36
Transmembranei85 – 107HelicalSequence analysisAdd BLAST23
Topological domaini108 – 113CytoplasmicSequence analysis6
Transmembranei114 – 137HelicalSequence analysisAdd BLAST24
Topological domaini138 – 145ExtracellularSequence analysis8
Transmembranei146 – 168HelicalSequence analysisAdd BLAST23
Topological domaini169 – 180CytoplasmicSequence analysisAdd BLAST12
Transmembranei181 – 203HelicalSequence analysisAdd BLAST23
Topological domaini204 – 212ExtracellularSequence analysis9
Transmembranei213 – 236HelicalSequence analysisAdd BLAST24
Topological domaini237 – 265CytoplasmicSequence analysisAdd BLAST29
Transmembranei266 – 288HelicalSequence analysisAdd BLAST23
Topological domaini289 – 309ExtracellularSequence analysisAdd BLAST21
Transmembranei310 – 328HelicalSequence analysisAdd BLAST19
Topological domaini329 – 331CytoplasmicSequence analysis3
Transmembranei332 – 356HelicalSequence analysisAdd BLAST25
Topological domaini357 – 359ExtracellularSequence analysis3
Transmembranei360 – 381HelicalSequence analysisAdd BLAST22
Topological domaini382 – 393CytoplasmicSequence analysisAdd BLAST12
Transmembranei394 – 412HelicalSequence analysisAdd BLAST19
Topological domaini413 – 424ExtracellularSequence analysisAdd BLAST12
Transmembranei425 – 449HelicalSequence analysisAdd BLAST25
Topological domaini450 – 459CytoplasmicSequence analysis10

GO - Cellular componenti

  • apical plasma membrane Source: UniProtKB
  • brush border membrane Source: Ensembl
  • cell surface Source: MGI
  • cytoplasm Source: UniProtKB
  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000848521 – 459Proton-coupled folate transporterAdd BLAST459

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei6PhosphoserineBy similarity1
Glycosylationi58N-linked (GlcNAc...)1 Publication1
Glycosylationi68N-linked (GlcNAc...)Sequence analysis1
Modified residuei458PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ6PEM8.
PaxDbiQ6PEM8.
PRIDEiQ6PEM8.

PTM databases

iPTMnetiQ6PEM8.
PhosphoSitePlusiQ6PEM8.

Expressioni

Tissue specificityi

Highly expressed in duodenum, especially in duodenal mucosa, the main site of intestinal heme absorption. Weakly expressed in the kidney. Not expressed in duodenum before weaning or in placenta. Weakly or not expressed in brain, heart, lung, skeletal muscle, testis and neonatal liver.1 Publication

Inductioni

Up-regulated in response to hypoxia, it is however unclear whether such up-regulation is direct or not. Not induced in the duodenum of iron-deficient mice.1 Publication

Gene expression databases

BgeeiENSMUSG00000020829.
CleanExiMM_SLC46A1.
ExpressionAtlasiQ6PEM8. baseline and differential.
GenevisibleiQ6PEM8. MM.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000001126.

Structurei

3D structure databases

ProteinModelPortaliQ6PEM8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2816. Eukaryota.
ENOG410ZVCA. LUCA.
GeneTreeiENSGT00530000063076.
HOGENOMiHOG000054191.
HOVERGENiHBG055334.
InParanoidiQ6PEM8.
KOiK14613.
OMAiTRHRENC.
OrthoDBiEOG091G08MP.
PhylomeDBiQ6PEM8.
TreeFamiTF315701.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR011701. MFS.
IPR020846. MFS_dom.
IPR005829. Sugar_transporter_CS.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6PEM8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEGRVSSVGS PHSFLNAPVL FRGPVEPLVF LANFALVLQG PLTTQYLWHR
60 70 80 90 100
FSTELGYNGT RHRENCGNQS ADPLMKEVET LTSHWTLYMN VGGFLVGLFW
110 120 130 140 150
STLLGAWSDR VGRRPLLVLA SLGLLLQAVV SIFVVQLELH VGFFVLGRAL
160 170 180 190 200
CALLGDFNGL LAASFASVAD VSSNHSRTFR MALLEACIGV AGTLASLLGG
210 220 230 240 250
HWLRAQGYAN PFWLALALLI VMALYAAFCF GETVKEPKST RLFTLRHHRS
260 270 280 290 300
IARLYVVPAP EKSRMHLALY SLAIFVVVTV HFGAQDILTL YELSAPLCWD
310 320 330 340 350
SKLIGYGSAA QHLPYLTSLL GLRLLQFCLA DTWVAEIGLA FNILGMVVFA
360 370 380 390 400
FATITPLMFT GYGLLFLSLV TTPVIRAKLS KLVSESEQGA LFSAVACVNS
410 420 430 440 450
LAMLMASGIF NSIYPATLNF MKGFPFLLGA GLLFIPAILI GVLEKVNPHP

EFQQFPQSP
Length:459
Mass (Da):50,089
Last modified:July 5, 2004 - v1
Checksum:iA251124B3B9846AF
GO

Sequence cautioni

The sequence BAD90126 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAI25543 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti402A → V in BAB22685 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK003278 mRNA. Translation: BAB22685.1.
AK170505 mRNA. Translation: BAE41843.1.
AK220201 mRNA. Translation: BAD90126.1. Different initiation.
AL591177 Genomic DNA. Translation: CAI25542.1.
AL591177 Genomic DNA. Translation: CAI25543.1. Sequence problems.
BC024522 mRNA. Translation: AAH24522.1.
BC057976 mRNA. Translation: AAH57976.1.
CCDSiCCDS25104.1.
RefSeqiNP_081016.2. NM_026740.2.
UniGeneiMm.131618.

Genome annotation databases

EnsembliENSMUST00000001126; ENSMUSP00000001126; ENSMUSG00000020829.
GeneIDi52466.
KEGGimmu:52466.
UCSCiuc007kjg.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK003278 mRNA. Translation: BAB22685.1.
AK170505 mRNA. Translation: BAE41843.1.
AK220201 mRNA. Translation: BAD90126.1. Different initiation.
AL591177 Genomic DNA. Translation: CAI25542.1.
AL591177 Genomic DNA. Translation: CAI25543.1. Sequence problems.
BC024522 mRNA. Translation: AAH24522.1.
BC057976 mRNA. Translation: AAH57976.1.
CCDSiCCDS25104.1.
RefSeqiNP_081016.2. NM_026740.2.
UniGeneiMm.131618.

3D structure databases

ProteinModelPortaliQ6PEM8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000001126.

PTM databases

iPTMnetiQ6PEM8.
PhosphoSitePlusiQ6PEM8.

Proteomic databases

MaxQBiQ6PEM8.
PaxDbiQ6PEM8.
PRIDEiQ6PEM8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000001126; ENSMUSP00000001126; ENSMUSG00000020829.
GeneIDi52466.
KEGGimmu:52466.
UCSCiuc007kjg.2. mouse.

Organism-specific databases

CTDi113235.
MGIiMGI:1098733. Slc46a1.

Phylogenomic databases

eggNOGiKOG2816. Eukaryota.
ENOG410ZVCA. LUCA.
GeneTreeiENSGT00530000063076.
HOGENOMiHOG000054191.
HOVERGENiHBG055334.
InParanoidiQ6PEM8.
KOiK14613.
OMAiTRHRENC.
OrthoDBiEOG091G08MP.
PhylomeDBiQ6PEM8.
TreeFamiTF315701.

Enzyme and pathway databases

ReactomeiR-MMU-196757. Metabolism of folate and pterines.
R-MMU-917937. Iron uptake and transport.

Miscellaneous databases

ChiTaRSiSlc46a1. mouse.
PROiQ6PEM8.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000020829.
CleanExiMM_SLC46A1.
ExpressionAtlasiQ6PEM8. baseline and differential.
GenevisibleiQ6PEM8. MM.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR011701. MFS.
IPR020846. MFS_dom.
IPR005829. Sugar_transporter_CS.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPCFT_MOUSE
AccessioniPrimary (citable) accession number: Q6PEM8
Secondary accession number(s): Q571I8
, Q5SYG0, Q8R1H7, Q9D1P1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: July 5, 2004
Last modified: November 2, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.