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Q6PEE3

- RIR2B_MOUSE

UniProt

Q6PEE3 - RIR2B_MOUSE

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Protein
Ribonucleoside-diphosphate reductase subunit M2 B
Gene
Rrm2b, P53r2
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Plays a pivotal role in cell survival by repairing damaged DNA in a p53/TP53-dependent manner. Supplies deoxyribonucleotides for DNA repair in cells arrested at G1 or G2. Contains an iron-tyrosyl free radical center required for catalysis. Forms an active ribonucleotide reductase (RNR) complex with RRM1 which is expressed both in resting and proliferating cells in response to DNA damage.2 Publications

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Cofactori

Binds 2 iron ions per subunit By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi100 – 1001Iron 1 By similarity
Metal bindingi131 – 1311Iron 1 By similarity
Metal bindingi131 – 1311Iron 2 By similarity
Metal bindingi134 – 1341Iron 1 By similarity
Active sitei138 – 1381 By similarity
Metal bindingi194 – 1941Iron 2 By similarity
Metal bindingi228 – 2281Iron 2 By similarity
Metal bindingi231 – 2311Iron 2 By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: MGI

GO - Biological processi

  1. DNA repair Source: MGI
  2. deoxyribonucleoside diphosphate metabolic process Source: InterPro
  3. deoxyribonucleoside triphosphate metabolic process Source: MGI
  4. deoxyribonucleotide biosynthetic process Source: MGI
  5. kidney development Source: MGI
  6. mitochondrial DNA replication Source: MGI
  7. negative regulation of intrinsic apoptotic signaling pathway by p53 class mediator Source: MGI
  8. renal system process Source: MGI
  9. response to oxidative stress Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA damage, DNA repair, DNA replication

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase subunit M2 B (EC:1.17.4.1)
Alternative name(s):
TP53-inducible ribonucleotide reductase M2 B
p53-inducible ribonucleotide reductase small subunit 2-like protein
Short name:
p53R2
Gene namesi
Name:Rrm2b
Synonyms:P53r2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 15

Organism-specific databases

MGIiMGI:2155865. Rrm2b.

Subcellular locationi

Cytoplasm By similarity. Nucleus By similarity
Note: Translocates from cytoplasm to nucleus in response to DNA damage By similarity.

GO - Cellular componenti

  1. mitochondrion Source: MGI
  2. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice develop normally until they are weaned but from then on exhibit growth retardation and early mortality. Pathological examination indicates that multiple organs fail and that they die from severe renal failure by the age of 14 weeks.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 351351Ribonucleoside-diphosphate reductase subunit M2 B
PRO_0000228152Add
BLAST

Proteomic databases

PaxDbiQ6PEE3.
PRIDEiQ6PEE3.

PTM databases

PhosphoSiteiQ6PEE3.

Expressioni

Gene expression databases

ArrayExpressiQ6PEE3.
BgeeiQ6PEE3.
CleanExiMM_RRM2B.
GenevestigatoriQ6PEE3.

Interactioni

Subunit structurei

Heterotetramer with large (RRM1) subunit. Interacts with p53/TP53. Interacts with RRM1 in response to DNA damage By similarity.1 Publication

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000022901.

Structurei

3D structure databases

ProteinModelPortaliQ6PEE3.
SMRiQ6PEE3. Positions 28-314.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0208.
GeneTreeiENSGT00390000013305.
HOGENOMiHOG000255975.
HOVERGENiHBG001647.
InParanoidiQ6PEE3.
KOiK10808.
OMAiCLMFHYL.
OrthoDBiEOG7VMP5N.
PhylomeDBiQ6PEE3.
TreeFamiTF300465.

Family and domain databases

Gene3Di1.10.620.20. 1 hit.
InterProiIPR009078. Ferritin-like_SF.
IPR012348. RNR-rel.
IPR000358. RNR_small.
[Graphical view]
PANTHERiPTHR23409. PTHR23409. 1 hit.
PfamiPF00268. Ribonuc_red_sm. 1 hit.
[Graphical view]
SUPFAMiSSF47240. SSF47240. 1 hit.
PROSITEiPS00368. RIBORED_SMALL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6PEE3-1 [UniParc]FASTAAdd to Basket

« Hide

MGDPERPEAA RPEKGEQLCS ETEENVVRSN EEPLLRKSSR RFVIFPIQYP    50
DIWRMYKQAQ ASFWTAEEVD LSKDLPHWNK LKSDEKYFIS HILAFFAASD 100
GIVNENLVER FSQEVQVPEA RCFYGFQILI ENVHSEMYSL LIDTYIRDPK 150
KREFLFNAIE TMPYVKKKAD WALRWIADRK STFGERVVAF AAVEGIFFSG 200
SFAAIFWLKK RGLMPGLTFS NELISRDEGL HCDFACLMFQ YLVNKPSEDR 250
VREIIADAVQ IEQEFLTEAL PVGLIGMNCV LMKQYIEFVA DRLLGELGFS 300
KIFQAENPFD FMENISLEGK TNFFEKRVSE YQRFAVMAET TDNVFTLDAD 350
F 351
Length:351
Mass (Da):40,804
Last modified:July 5, 2004 - v1
Checksum:i4E1259233C9CC8A9
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK138731 mRNA. Translation: BAE23760.1.
BC058103 mRNA. Translation: AAH58103.1.
CCDSiCCDS27437.1.
RefSeqiNP_955770.1. NM_199476.1.
UniGeneiMm.24738.

Genome annotation databases

EnsembliENSMUST00000022901; ENSMUSP00000022901; ENSMUSG00000022292.
GeneIDi382985.
KEGGimmu:382985.
UCSCiuc007vnl.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK138731 mRNA. Translation: BAE23760.1 .
BC058103 mRNA. Translation: AAH58103.1 .
CCDSi CCDS27437.1.
RefSeqi NP_955770.1. NM_199476.1.
UniGenei Mm.24738.

3D structure databases

ProteinModelPortali Q6PEE3.
SMRi Q6PEE3. Positions 28-314.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10090.ENSMUSP00000022901.

PTM databases

PhosphoSitei Q6PEE3.

Proteomic databases

PaxDbi Q6PEE3.
PRIDEi Q6PEE3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000022901 ; ENSMUSP00000022901 ; ENSMUSG00000022292 .
GeneIDi 382985.
KEGGi mmu:382985.
UCSCi uc007vnl.1. mouse.

Organism-specific databases

CTDi 50484.
MGIi MGI:2155865. Rrm2b.

Phylogenomic databases

eggNOGi COG0208.
GeneTreei ENSGT00390000013305.
HOGENOMi HOG000255975.
HOVERGENi HBG001647.
InParanoidi Q6PEE3.
KOi K10808.
OMAi CLMFHYL.
OrthoDBi EOG7VMP5N.
PhylomeDBi Q6PEE3.
TreeFami TF300465.

Enzyme and pathway databases

UniPathwayi UPA00326 .

Miscellaneous databases

NextBioi 403612.
PROi Q6PEE3.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q6PEE3.
Bgeei Q6PEE3.
CleanExi MM_RRM2B.
Genevestigatori Q6PEE3.

Family and domain databases

Gene3Di 1.10.620.20. 1 hit.
InterProi IPR009078. Ferritin-like_SF.
IPR012348. RNR-rel.
IPR000358. RNR_small.
[Graphical view ]
PANTHERi PTHR23409. PTHR23409. 1 hit.
Pfami PF00268. Ribonuc_red_sm. 1 hit.
[Graphical view ]
SUPFAMi SSF47240. SSF47240. 1 hit.
PROSITEi PS00368. RIBORED_SMALL. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Thymus.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain.
  3. "Mammalian p53R2 protein forms an active ribonucleotide reductase in vitro with the R1 protein, which is expressed both in resting cells in response to DNA damage and in proliferating cells."
    Guittet O., Haakansson P., Voevodskaya N., Fridd S., Graeslund A., Arakawa H., Nakamura Y., Thelander L.
    J. Biol. Chem. 276:40647-40651(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT.
  4. "Impaired function of p53R2 in Rrm2b-null mice causes severe renal failure through attenuation of dNTP pools."
    Kimura T., Takeda S., Sagiya Y., Gotoh M., Nakamura Y., Arakawa H.
    Nat. Genet. 34:440-445(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiRIR2B_MOUSE
AccessioniPrimary (citable) accession number: Q6PEE3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 21, 2006
Last sequence update: July 5, 2004
Last modified: July 9, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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