Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ribonucleoside-diphosphate reductase subunit M2 B

Gene

Rrm2b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a pivotal role in cell survival by repairing damaged DNA in a p53/TP53-dependent manner. Supplies deoxyribonucleotides for DNA repair in cells arrested at G1 or G2. Contains an iron-tyrosyl free radical center required for catalysis. Forms an active ribonucleotide reductase (RNR) complex with RRM1 which is expressed both in resting and proliferating cells in response to DNA damage.2 Publications

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.PROSITE-ProRule annotation

Cofactori

Fe cationBy similarityNote: Binds 2 iron ions per subunit.By similarity

Pathwayi: DNA replication

This protein is involved in the pathway DNA replication, which is part of Genetic information processing.
View all proteins of this organism that are known to be involved in the pathway DNA replication and in Genetic information processing.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi100Iron 1PROSITE-ProRule annotation1
Metal bindingi131Iron 1PROSITE-ProRule annotation1
Metal bindingi131Iron 2By similarity1
Metal bindingi134Iron 1PROSITE-ProRule annotation1
Active sitei138PROSITE-ProRule annotation1
Metal bindingi194Iron 2By similarity1
Metal bindingi228Iron 2By similarity1
Metal bindingi231Iron 2By similarity1

GO - Molecular functioni

GO - Biological processi

  • deoxyribonucleoside triphosphate metabolic process Source: MGI
  • deoxyribonucleotide biosynthetic process Source: MGI
  • DNA repair Source: MGI
  • kidney development Source: MGI
  • mitochondrial DNA replication Source: MGI
  • negative regulation of intrinsic apoptotic signaling pathway by p53 class mediator Source: MGI
  • renal system process Source: MGI
  • response to amine Source: Ensembl
  • response to oxidative stress Source: MGI

Keywordsi

Molecular functionOxidoreductase
Biological processDNA damage, DNA repair, DNA replication
LigandIron, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00326

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase subunit M2 B (EC:1.17.4.1)
Alternative name(s):
TP53-inducible ribonucleotide reductase M2 B
p53-inducible ribonucleotide reductase small subunit 2-like protein
Short name:
p53R2
Gene namesi
Name:Rrm2b
Synonyms:P53r2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:2155865 Rrm2b

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice develop normally until they are weaned but from then on exhibit growth retardation and early mortality. Pathological examination indicates that multiple organs fail and that they die from severe renal failure by the age of 14 weeks.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002281521 – 351Ribonucleoside-diphosphate reductase subunit M2 BAdd BLAST351

Proteomic databases

EPDiQ6PEE3
MaxQBiQ6PEE3
PaxDbiQ6PEE3
PRIDEiQ6PEE3

PTM databases

iPTMnetiQ6PEE3
PhosphoSitePlusiQ6PEE3

Expressioni

Gene expression databases

BgeeiENSMUSG00000022292
CleanExiMM_RRM2B
ExpressionAtlasiQ6PEE3 baseline and differential
GenevisibleiQ6PEE3 MM

Interactioni

Subunit structurei

Heterotetramer with large (RRM1) subunit. Interacts with p53/TP53. Interacts with RRM1 in response to DNA damage (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000022901

Structurei

3D structure databases

ProteinModelPortaliQ6PEE3
SMRiQ6PEE3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1567 Eukaryota
COG0208 LUCA
GeneTreeiENSGT00390000013305
HOGENOMiHOG000255975
HOVERGENiHBG001647
InParanoidiQ6PEE3
KOiK10808
OMAiSEEKYFI
OrthoDBiEOG091G0AZQ
PhylomeDBiQ6PEE3
TreeFamiTF300465

Family and domain databases

CDDicd01049 RNRR2, 1 hit
Gene3Di1.10.620.20, 1 hit
InterProiView protein in InterPro
IPR009078 Ferritin-like_SF
IPR012348 RNR-like
IPR033909 RNR_small
IPR030475 RNR_small_AS
IPR000358 RNR_small_fam
PANTHERiPTHR23409 PTHR23409, 1 hit
PfamiView protein in Pfam
PF00268 Ribonuc_red_sm, 1 hit
PIRSFiPIRSF000355 NrdB, 1 hit
SUPFAMiSSF47240 SSF47240, 1 hit
PROSITEiView protein in PROSITE
PS00368 RIBORED_SMALL, 1 hit

Sequencei

Sequence statusi: Complete.

Q6PEE3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGDPERPEAA RPEKGEQLCS ETEENVVRSN EEPLLRKSSR RFVIFPIQYP
60 70 80 90 100
DIWRMYKQAQ ASFWTAEEVD LSKDLPHWNK LKSDEKYFIS HILAFFAASD
110 120 130 140 150
GIVNENLVER FSQEVQVPEA RCFYGFQILI ENVHSEMYSL LIDTYIRDPK
160 170 180 190 200
KREFLFNAIE TMPYVKKKAD WALRWIADRK STFGERVVAF AAVEGIFFSG
210 220 230 240 250
SFAAIFWLKK RGLMPGLTFS NELISRDEGL HCDFACLMFQ YLVNKPSEDR
260 270 280 290 300
VREIIADAVQ IEQEFLTEAL PVGLIGMNCV LMKQYIEFVA DRLLGELGFS
310 320 330 340 350
KIFQAENPFD FMENISLEGK TNFFEKRVSE YQRFAVMAET TDNVFTLDAD

F
Length:351
Mass (Da):40,804
Last modified:July 5, 2004 - v1
Checksum:i4E1259233C9CC8A9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK138731 mRNA Translation: BAE23760.1
BC058103 mRNA Translation: AAH58103.1
CCDSiCCDS27437.1
RefSeqiNP_955770.1, NM_199476.1
UniGeneiMm.24738

Genome annotation databases

EnsembliENSMUST00000022901; ENSMUSP00000022901; ENSMUSG00000022292
GeneIDi382985
KEGGimmu:382985
UCSCiuc007vnl.1 mouse

Similar proteinsi

Entry informationi

Entry nameiRIR2B_MOUSE
AccessioniPrimary (citable) accession number: Q6PEE3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 21, 2006
Last sequence update: July 5, 2004
Last modified: March 28, 2018
This is version 127 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health