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Protein

CBP80/20-dependent translation initiation factor

Gene

Ctif

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Specifically required for the pioneer round of mRNA translation mediated by the cap-binding complex (CBC), that takes place during or right after mRNA export via the nuclear pore complex (NPC). Acts via its interaction with the NCBP1/CBP80 component of the CBC complex and recruits the 40S small subunit of the ribosome via eIF3. In contrast, it is not involved in steady state translation, that takes place when the CBC complex is replaced by cytoplasmic cap-binding protein eIF4E. Also required for nonsense-mediated mRNA decay (NMD), the pioneer round of mRNA translation mediated by the cap-binding complex playing a central role in nonsense-mediated mRNA decay (NMD) (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Nonsense-mediated mRNA decay, Translation regulation

Names & Taxonomyi

Protein namesi
Recommended name:
CBP80/20-dependent translation initiation factor
Gene namesi
Name:Ctif
Synonyms:Gm672, Kiaa0427
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:2685518. Ctif.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 600600CBP80/20-dependent translation initiation factorPRO_0000050755Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei18 – 181PhosphoserineCombined sources
Modified residuei289 – 2891PhosphothreonineBy similarity
Modified residuei299 – 2991PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ6PEE2.
PaxDbiQ6PEE2.
PRIDEiQ6PEE2.

PTM databases

iPTMnetiQ6PEE2.

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Gene expression databases

CleanExiMM_GM672.

Interactioni

Subunit structurei

Interacts with NCBP1/CBP80; the interaction is direct. Associates with the eukaryotic translation initiation factor 3 (eIF-3) complex (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000129974.

Structurei

3D structure databases

ProteinModelPortaliQ6PEE2.
SMRiQ6PEE2. Positions 377-591.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini378 – 579202MIF4GAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 305305Interaction with NCBP1/CBP80By similarityAdd
BLAST

Sequence similaritiesi

Belongs to the CTIF family.Curated
Contains 1 MIF4G domain.Curated

Phylogenomic databases

eggNOGiKOG3942. Eukaryota.
ENOG410XQKW. LUCA.
HOGENOMiHOG000113148.
InParanoidiQ6PEE2.
PhylomeDBiQ6PEE2.

Family and domain databases

Gene3Di1.25.40.180. 1 hit.
InterProiIPR016024. ARM-type_fold.
IPR016021. MIF4-like.
IPR003890. MIF4G-like_typ-3.
[Graphical view]
PfamiPF02854. MIF4G. 1 hit.
[Graphical view]
SMARTiSM00543. MIF4G. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6PEE2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MENSSAASAS SEAGSSRSQE IEELERFIDS YVLEYQVQGL LTDKTEGDGE
60 70 80 90 100
SQRTQSHISQ WTADCREQLD GSCSFSRGRA PPQQNGNKDN SLDMLGTDIW
110 120 130 140 150
AANTFDSFSG ATWDLQPEKL DFTQFHRKVR HTPKQPLPHI DREGCGKGKL
160 170 180 190 200
EDGDGISLND IEKVLPTWQG YHPMPHEAEI AHTKKLFRRR RNDRRRQQRP
210 220 230 240 250
PGGNKPQQHG DHQPGSAKHN RDHQKSYQGG SGPHPSGRPT HHGYSQNRRW
260 270 280 290 300
HHGNMKHPPG DKGEAGSHRN AKETVTVENP KLEDGPGDTG HSGLEPPCSP
310 320 330 340 350
DTLTPAASER PTPQLPGGPE AEIKHKDTVL PERLRERPKI TLLQSSKDRL
360 370 380 390 400
RRRLKEKDRD EVAVETSSPQ PSKMDRLMEI LNIMRNNSSD VDAKLTSFME
410 420 430 440 450
EAQNSTNSEE MLGEIVRTIY QKAVSDRSFA FTAAKLCDKM ALFMVEGTKF
460 470 480 490 500
RSLLLNMLQK DFTVREELQQ QDVERWLGFI TFLCEVFGTM RSSTGEPFRV
510 520 530 540 550
LVCPIYTCLR ELLQSQDVKE DAVLCCSMEL QSTGRLLEEQ LPEMMTELLA
560 570 580 590 600
SARDKMLCPS ESMLTRSLLL EVIELHANSW NPLTPPITQY YNRTIQKLTA
Length:600
Mass (Da):67,831
Last modified:September 22, 2009 - v2
Checksum:iC648CFDEBC94D8AD
GO
Isoform 2 (identifier: Q6PEE2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     359-359: R → RDIPNPTETSAPLRCVLCVPHVPQ

Note: No experimental confirmation available.
Show »
Length:623
Mass (Da):70,300
Checksum:iD5E5950519430375
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti383 – 3831I → S in BAD32227 (PubMed:15368895).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei359 – 3591R → RDIPNPTETSAPLRCVLCVP HVPQ in isoform 2. 1 PublicationVSP_038120

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC058104 mRNA. Translation: AAH58104.1.
AK172949 mRNA. Translation: BAD32227.1.
CCDSiCCDS37861.1. [Q6PEE2-2]
RefSeqiNP_958742.2. NM_201354.2.
UniGeneiMm.36745.

Genome annotation databases

GeneIDi269037.
KEGGimmu:269037.
UCSCiuc008fqf.2. mouse. [Q6PEE2-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC058104 mRNA. Translation: AAH58104.1.
AK172949 mRNA. Translation: BAD32227.1.
CCDSiCCDS37861.1. [Q6PEE2-2]
RefSeqiNP_958742.2. NM_201354.2.
UniGeneiMm.36745.

3D structure databases

ProteinModelPortaliQ6PEE2.
SMRiQ6PEE2. Positions 377-591.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000129974.

PTM databases

iPTMnetiQ6PEE2.

Proteomic databases

MaxQBiQ6PEE2.
PaxDbiQ6PEE2.
PRIDEiQ6PEE2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi269037.
KEGGimmu:269037.
UCSCiuc008fqf.2. mouse. [Q6PEE2-1]

Organism-specific databases

CTDi9811.
MGIiMGI:2685518. Ctif.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG3942. Eukaryota.
ENOG410XQKW. LUCA.
HOGENOMiHOG000113148.
InParanoidiQ6PEE2.
PhylomeDBiQ6PEE2.

Miscellaneous databases

NextBioi392656.
PROiQ6PEE2.
SOURCEiSearch...

Gene expression databases

CleanExiMM_GM672.

Family and domain databases

Gene3Di1.25.40.180. 1 hit.
InterProiIPR016024. ARM-type_fold.
IPR016021. MIF4-like.
IPR003890. MIF4G-like_typ-3.
[Graphical view]
PfamiPF02854. MIF4G. 1 hit.
[Graphical view]
SMARTiSM00543. MIF4G. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6J.
    Tissue: Brain.
  2. "Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H., Nagase T., Ohara O., Koga H.
    DNA Res. 11:205-218(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 334-600 (ISOFORM 1).
    Tissue: Pancreatic islet.
  3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-18, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  4. "A new MIF4G domain-containing protein, CTIF, directs nuclear cap-binding protein CBP80/20-dependent translation."
    Kim K.M., Cho H., Choi K., Kim J., Kim B.-W., Ko Y.-G., Jang S.K., Kim Y.K.
    Genes Dev. 23:2033-2045(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiCTIF_MOUSE
AccessioniPrimary (citable) accession number: Q6PEE2
Secondary accession number(s): Q6A069
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 24, 2005
Last sequence update: September 22, 2009
Last modified: January 20, 2016
This is version 82 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.