Reviewed, UniProtKB/Swiss-Prot Q6PEC0 (AP4A_RAT)

Names and origin

Protein names

Recommended name:
    Bis(5'-nucleosyl)-tetraphosphatase [asymmetrical]
    EC=3.6.1.17
Alternative name(s):
    Diadenosine 5',5'''-P1,P4-tetraphosphate asymmetrical hydrolase
      Short name=Diadenosine tetraphosphatase
      Short name=Ap4A hydrolase
      Short name=Ap4Aase
    Nucleoside diphosphate-linked moiety X motif 2
      Short name=Nudix motif 2

Gene names

Name:

Nudt2

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	147 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Asymmetrically hydrolyzes Ap4A to yield AMP and ATP. Plays a major role in maintaining homeostasis By similarity.
Catalytic activity	P1,P(4)-bis(5'-guanosyl) tetraphosphate + H2O = GTP + GMP.
Cofactor	Divalent ions By similarity.
Sequence similarities	Belongs to the Nudix hydrolase family. Contains 1 nudix hydrolase domain.

Ontologies

Keywords
Ligand	GTP-binding Nucleotide-binding
Molecular function	Hydrolase
PTM	Acetylation
Gene Ontology (GO)
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW bis(5'-nucleosyl)-tetraphosphatase (asymmetrical) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 147	146	Bis(5'-nucleosyl)-tetraphosphatase [asymmetrical]		PRO_0000057105
Regions
	Domain	1 – 139	139	Nudix hydrolase
	Motif	43 – 64	22	Nudix box
Amino acid modifications
	Modified residue	2	1	N-acetylalanine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6PEC0-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: F17F2BB44D2B9C33	FASTA	147	16,929
	10 20 30 40 50 60 MALRACGLII FRRHLIPKVD NTTIEFLLLQ ASDGIHHWTP PKGHVDPGEN DLETALRETQ 70 80 90 100 110 120 EETGIEASQL IVLEGFRREL NYVARKKPKT VIYWLAEVKD YDVEIRLSQE HQAYRWLGLD 130 140 EACQLAQFEE MKATLQEGHQ FLCSTPA

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Pituitary.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC058156 mRNA. Translation: AAH58156.1.
IPI	IPI00195619.
RefSeq	NP_997479.1.
UniGene	Rn.40265
3D structure databases
SMR	Q6PEC0. Positions 1-147.
ModBase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000017712; ENSRNOP00000017712; ENSRNOG00000013110; Rattus norvegicus. [Genome view]
GeneID	297998.
KEGG	rno:297998.
NMPDR	fig|10116.3.peg.22986.
UCSC	NM_207596. rat.
Organism-specific databases
CTD	297998.
RGD	1303023. Nudt2.
Phylogenomic databases
HOVERGEN	Q6PEC0.
OMA	HQFLCST.
Enzyme and pathway databases
BRENDA	3.6.1.17. 248.
Gene expression databases
ArrayExpress	Q6PEC0.
Genevestigator	Q6PEC0.
GermOnline	ENSRNOG00000013110. Rattus norvegicus.
Family and domain databases
InterPro	IPR020476. Nudix_hydrolase. IPR020084. NUDIX_hydrolase_CS. IPR000086. NUDIX_hydrolase_dom. IPR003565. Tetra_PHTase. [Graphical view]
Gene3D	G3DSA:3.90.79.10. NUDIX_hydrolase. 1 hit.
Pfam	PF00293. NUDIX. 1 hit. [Graphical view]
PRINTS	PR00502. NUDIXFAMILY. PR01405. TETRPHPHTASE.
PROSITE	PS51462. NUDIX. 1 hit. PS00893. NUDIX_BOX. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	642939.

Entry information

Entry name

AP4A_RAT

Accession

Primary (citable) accession number: Q6PEC0

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	January 23, 2007
Last modified:	November 3, 2009
This is version 45 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)