Reviewed, UniProtKB/Swiss-Prot Q6PE18 (P4K2A_DANRE)

Names and origin

Protein names

Recommended name:
    Phosphatidylinositol 4-kinase type 2-alpha
    EC=2.7.1.67
Alternative name(s):
    Phosphatidylinositol 4-kinase type II-alpha

Gene names

Name:	pi4k2a
Synonyms:	pi4kii
ORF Names:	si:ch211-197k17.1

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	447 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Contributes to the overall PI4-kinase activity of the cell. The phosphorylation of phosphatidylinositol (PI) to PI4P is the first committed step in the generation of phosphatidylinositol 4,5-bisphosphate (PIP2), a precursor of the second messenger inositol 1,4,5-trisphosphate (InsP3) By similarity.
Catalytic activity	ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate.
Subcellular location	Cytoplasm By similarity. Membrane By similarity.
Sequence similarities	Belongs to the PI3/PI4-kinase family. Type II PI4K subfamily. Contains 1 PI3K/PI4K domain.

Ontologies

Keywords
Cellular component	Cytoplasm Membrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	1-phosphatidylinositol 4-kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 447	447	Phosphatidylinositol 4-kinase type 2-alpha		PRO_0000285161
Regions
	Domain	101 – 406	306	PI3K/PI4K
Experimental info
	Sequence conflict	255	1	N → S in AAX89135. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q6PE18-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 305A7326FF293865	FASTA	447	51,443
	10 20 30 40 50 60 MDETSPLVSP LRDSNDFNYG PAEPTSPRGG FGSTPGSVVR LPAGSPGRSR ERQPLLDRDR 70 80 90 100 110 120 GASPRDPHRN EFPEDPEFRE IIRKAERAIE EGIYPERIYQ GSSGSYFVKD SAGKIIGVFK 130 140 150 160 170 180 PKNEEPYGQL NPKWTKWLQK LCCPCCFGRD CLVLNQGYLS EAGASLVDQK LELNIVPRTK 190 200 210 220 230 240 VVYLASETFN YSAIDRVKSR GKRLALEKVP KVGQRFHRIG LPPKVGSFQI FVEGYKDADF 250 260 270 280 290 300 WLRRFEAEPL PENTNRQLQL QFERLVVLDY IIRNTDRGND NWLIKYDYPM DTSSNRDSDW 310 320 330 340 350 360 VLVKDPIIKL AAIDNGLAFP LKHPDSWRAY PFYWAWLPQA KVVFSQEIRD LVLPKLADPN 370 380 390 400 410 420 FIKDLEEDLY ELFKKDPGFD RGQFKKQVSV MRGQILNLSQ AMRDGKTPLQ LVQMPPVIVE 430 440 TARVPQRANS ESYTQSFQSR RPFFTWW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Phosphatidylinositol 4-kinases of Danio rerio." Ma H., Balla T. Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	The Danio rerio sequencing project at the Sanger Institute Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tuebingen.
[3]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: SJD.
[4]	"Cloning of Danio rerio phosphatidylinositol 4-kinase type II partial cDNA." Rafael M.S., Laize V., Cancela M.L. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 105-447.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY929290 mRNA. Translation: AAY16565.1. AL929041, BX119918 Genomic DNA. Translation: CAN88146.1. BC058342 mRNA. Translation: AAH58342.1. AY989909 mRNA. Translation: AAX89135.1.
IPI	IPI00487489.
RefSeq	NP_998523.1.
UniGene	Dr.79494
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	Q6PE18.
Genome annotation databases
Ensembl	ENSDART00000048457; ENSDARP00000048456; ENSDARG00000033666; Danio rerio. [Genome view]
GeneID	406667.
KEGG	dre:406667.
Organism-specific databases
CTD	406667.
ZFIN	ZDB-GENE-040426-2675. pi4k2a.
Phylogenomic databases
HOVERGEN	Q6PE18.
OMA	IIKLAAI.
Enzyme and pathway databases
BRENDA	2.7.1.67. 96826.
Gene expression databases
Bgee	Q6PE18.
Family and domain databases
InterPro	IPR000403. PI3/4_kinase_cat. IPR018936. PI3/4_kinase_CS. [Graphical view]
Pfam	PF00454. PI3_PI4_kinase. 1 hit. [Graphical view]
PROSITE	PS00915. PI3_4_KINASE_1. False negative. PS00916. PI3_4_KINASE_2. False negative. PS50290. PI3_4_KINASE_3. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

P4K2A_DANRE

Accession

Primary (citable) accession number: Q6PE18
Secondary accession number(s): A5PF25, Q52PF3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 2007
Last sequence update:	July 5, 2004
Last modified:	November 3, 2009
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Zebrafish annotation project