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Protein

E3 ubiquitin-protein ligase Rnf220

Gene

Rnf220

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

E3 ubiquitin-protein ligase that promotes the ubiquitination and proteasomal degradation of SIN3B (PubMed:20170641). Independently of its E3 ligase activity, acts as a CTNNB1 stabilizer through USP7-mediated deubiquitination of CTNNB1 and promotes Wnt signaling (By similarity).By similarity1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri514 – 55340RING-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • ligase activity Source: UniProtKB-KW
  • ubiquitin protein ligase activity Source: MGI
  • ubiquitin-protein transferase activity Source: UniProtKB
  • zinc ion binding Source: InterPro

GO - Biological processi

  • positive regulation of canonical Wnt signaling pathway Source: UniProtKB
  • protein autoubiquitination Source: UniProtKB
  • protein ubiquitination Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BRENDAi2.3.2.B10. 3474.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase Rnf220 (EC:6.3.2.-)
Alternative name(s):
RING finger protein 220
Gene namesi
Name:Rnf220
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1913993. Rnf220.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 566566E3 ubiquitin-protein ligase Rnf220PRO_0000277659Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki277 – 277Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei390 – 3901PhosphoserineCombined sources

Post-translational modificationi

Auto-ubiquitinated; leads to proteasomal degradation.By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ6PDX6.
PaxDbiQ6PDX6.
PeptideAtlasiQ6PDX6.
PRIDEiQ6PDX6.

PTM databases

iPTMnetiQ6PDX6.
PhosphoSiteiQ6PDX6.

Expressioni

Gene expression databases

BgeeiENSMUSG00000028677.
ExpressionAtlasiQ6PDX6. baseline and differential.
GenevisibleiQ6PDX6. MM.

Interactioni

Subunit structurei

Interacts with SIN3B (PubMed:20170641). Interacts with CTNNB1 (via Armadillo repeats 2-8) (By similarity). Interacts with USP7 (via MATH domain) (By similarity).By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Sin3bQ621415EBI-2795840,EBI-591450

Protein-protein interaction databases

BioGridi211685. 1 interaction.
IntActiQ6PDX6. 6 interactions.
STRINGi10090.ENSMUSP00000030439.

Structurei

3D structure databases

ProteinModelPortaliQ6PDX6.
SMRiQ6PDX6. Positions 510-564.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni514 – 5229Required for targeting to the cytoplasm

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili485 – 51329Sequence analysisAdd
BLAST

Sequence similaritiesi

Contains 1 RING-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri514 – 55340RING-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiENOG410IEJC. Eukaryota.
ENOG4111JY9. LUCA.
GeneTreeiENSGT00390000016573.
HOGENOMiHOG000231748.
HOVERGENiHBG055526.
InParanoidiQ6PDX6.
OMAiSDRCKKK.
OrthoDBiEOG091G0PN3.
PhylomeDBiQ6PDX6.
TreeFamiTF324716.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR031824. RNF220_mid.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF15926. RNF220. 2 hits.
[Graphical view]
PROSITEiPS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6PDX6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDLHRAAFKM ENSSYLPNPL ASPALMVLAS TAEASRDASI PCQQPRPFGV
60 70 80 90 100
PVSVDKDVHI PFTNGSYTFA SMYHRQGGVP GTFANRDFPP SLLHLHPQFA
110 120 130 140 150
PPNLDCTPIS MLNHSGVGAF RPFASTEDRE SYQSAFTPAK RLKNCHDTES
160 170 180 190 200
PHLRFSDADG KEYDFGTQLP SSSPGSLKVD DTGKKIFAVS GLISDRETSS
210 220 230 240 250
SPEDRNDRCK KKAVALFDSQ APLCPICQVL LRPSELQEHM EQELEQLAQL
260 270 280 290 300
PASKNSLLKD AMAPGTPKSL LLSASIKREG DSPTASPHSS ATEDLHHSDR
310 320 330 340 350
YQTFLRVRAN RQTRLNARIG KMKRRKQDEG QREGSCMAED DAVDIEHADS
360 370 380 390 400
NRFEEYEWCG QKRIRATTLL EGGFRGSGFV MCSGKENPDS DADLDVDGDD
410 420 430 440 450
TLEYGKPQYT EADVIPCTGE EPGEAKEREA LRGAVLNGGP PSTRITPEFS
460 470 480 490 500
KWASDEMPST SNGEGSKQEA MQKTCKNSDI EKITEESAVT TFEALKARVR
510 520 530 540 550
ELERQLSRGD RYKCLICMDS YSMPLTSIQC WHVHCEECWL RTLGAKKLCP
560
QCNTITAPGD LRRIYL
Length:566
Mass (Da):62,693
Last modified:July 5, 2004 - v1
Checksum:iAC8D8B716E9E6BAA
GO
Isoform 2 (identifier: Q6PDX6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-284: Missing.
     285-302: ASPHSSATEDLHHSDRYQ → MPRARSGRRSRGADGREE
     331-331: Q → QVCPLCSRPLAGSEQEMSRHVEHCLAK

Show »
Length:308
Mass (Da):34,626
Checksum:iB98AFAB128F4439B
GO
Isoform 3 (identifier: Q6PDX6-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     211-239: KKAVALFDSQAPLCPICQVLLRPSELQEH → YFGWNPLLTPSKPASQKCSTGWQHRTCSQ
     240-566: Missing.

Note: No experimental confirmation available.
Show »
Length:239
Mass (Da):26,252
Checksum:i07FEA45670F940BA
GO
Isoform 4 (identifier: Q6PDX6-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-321: Missing.

Show »
Length:245
Mass (Da):27,484
Checksum:i516B7FB12B22F7BE
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti553 – 5531N → S in AAH34875 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 321321Missing in isoform 4. 1 PublicationVSP_023068Add
BLAST
Alternative sequencei1 – 284284Missing in isoform 2. 1 PublicationVSP_023069Add
BLAST
Alternative sequencei211 – 23929KKAVA…ELQEH → YFGWNPLLTPSKPASQKCST GWQHRTCSQ in isoform 3. 1 PublicationVSP_023070Add
BLAST
Alternative sequencei240 – 566327Missing in isoform 3. 1 PublicationVSP_023071Add
BLAST
Alternative sequencei285 – 30218ASPHS…SDRYQ → MPRARSGRRSRGADGREE in isoform 2. 1 PublicationVSP_023072Add
BLAST
Alternative sequencei331 – 3311Q → QVCPLCSRPLAGSEQEMSRH VEHCLAK in isoform 2. 1 PublicationVSP_023073

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK041785 mRNA. Translation: BAE20604.1.
AK172415 mRNA. Translation: BAE42996.1.
AL645740 Genomic DNA. Translation: CAM20839.1.
AL844169 Genomic DNA. Translation: CAM20837.1.
AL844169 Genomic DNA. Translation: CAM23616.1.
BC034875 mRNA. Translation: AAH34875.1.
BC056359 mRNA. Translation: AAH56359.1.
BC058415 mRNA. Translation: AAH58415.1.
CCDSiCCDS18534.1. [Q6PDX6-1]
CCDS80149.1. [Q6PDX6-4]
RefSeqiNP_001297658.1. NM_001310729.1.
NP_001297659.1. NM_001310730.1. [Q6PDX6-2]
NP_001297660.1. NM_001310731.1. [Q6PDX6-4]
NP_080015.3. NM_025739.3. [Q6PDX6-1]
XP_011238891.1. XM_011240589.1. [Q6PDX6-4]
XP_011238892.1. XM_011240590.1. [Q6PDX6-4]
UniGeneiMm.318331.
Mm.470234.

Genome annotation databases

EnsembliENSMUST00000030439; ENSMUSP00000030439; ENSMUSG00000028677. [Q6PDX6-1]
ENSMUST00000102690; ENSMUSP00000099751; ENSMUSG00000028677. [Q6PDX6-4]
GeneIDi66743.
KEGGimmu:66743.
UCSCiuc008uim.1. mouse. [Q6PDX6-4]
uc008uin.1. mouse. [Q6PDX6-2]
uc008uip.1. mouse. [Q6PDX6-1]
uc008uiq.1. mouse. [Q6PDX6-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK041785 mRNA. Translation: BAE20604.1.
AK172415 mRNA. Translation: BAE42996.1.
AL645740 Genomic DNA. Translation: CAM20839.1.
AL844169 Genomic DNA. Translation: CAM20837.1.
AL844169 Genomic DNA. Translation: CAM23616.1.
BC034875 mRNA. Translation: AAH34875.1.
BC056359 mRNA. Translation: AAH56359.1.
BC058415 mRNA. Translation: AAH58415.1.
CCDSiCCDS18534.1. [Q6PDX6-1]
CCDS80149.1. [Q6PDX6-4]
RefSeqiNP_001297658.1. NM_001310729.1.
NP_001297659.1. NM_001310730.1. [Q6PDX6-2]
NP_001297660.1. NM_001310731.1. [Q6PDX6-4]
NP_080015.3. NM_025739.3. [Q6PDX6-1]
XP_011238891.1. XM_011240589.1. [Q6PDX6-4]
XP_011238892.1. XM_011240590.1. [Q6PDX6-4]
UniGeneiMm.318331.
Mm.470234.

3D structure databases

ProteinModelPortaliQ6PDX6.
SMRiQ6PDX6. Positions 510-564.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi211685. 1 interaction.
IntActiQ6PDX6. 6 interactions.
STRINGi10090.ENSMUSP00000030439.

PTM databases

iPTMnetiQ6PDX6.
PhosphoSiteiQ6PDX6.

Proteomic databases

MaxQBiQ6PDX6.
PaxDbiQ6PDX6.
PeptideAtlasiQ6PDX6.
PRIDEiQ6PDX6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000030439; ENSMUSP00000030439; ENSMUSG00000028677. [Q6PDX6-1]
ENSMUST00000102690; ENSMUSP00000099751; ENSMUSG00000028677. [Q6PDX6-4]
GeneIDi66743.
KEGGimmu:66743.
UCSCiuc008uim.1. mouse. [Q6PDX6-4]
uc008uin.1. mouse. [Q6PDX6-2]
uc008uip.1. mouse. [Q6PDX6-1]
uc008uiq.1. mouse. [Q6PDX6-3]

Organism-specific databases

CTDi55182.
MGIiMGI:1913993. Rnf220.

Phylogenomic databases

eggNOGiENOG410IEJC. Eukaryota.
ENOG4111JY9. LUCA.
GeneTreeiENSGT00390000016573.
HOGENOMiHOG000231748.
HOVERGENiHBG055526.
InParanoidiQ6PDX6.
OMAiSDRCKKK.
OrthoDBiEOG091G0PN3.
PhylomeDBiQ6PDX6.
TreeFamiTF324716.

Enzyme and pathway databases

UniPathwayiUPA00143.
BRENDAi2.3.2.B10. 3474.

Miscellaneous databases

ChiTaRSiRnf220. mouse.
PROiQ6PDX6.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028677.
ExpressionAtlasiQ6PDX6. baseline and differential.
GenevisibleiQ6PDX6. MM.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR031824. RNF220_mid.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF15926. RNF220. 2 hits.
[Graphical view]
PROSITEiPS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRN220_MOUSE
AccessioniPrimary (citable) accession number: Q6PDX6
Secondary accession number(s): A2APP0
, Q3T9M4, Q3V3D7, Q8JZY5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: July 5, 2004
Last modified: September 7, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.