Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

E3 ubiquitin-protein ligase Rnf220

Gene

Rnf220

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

E3 ubiquitin-protein ligase that promotes the ubiquitination and proteasomal degradation of SIN3B (PubMed:20170641). Independently of its E3 ligase activity, acts as a CTNNB1 stabilizer through USP7-mediated deubiquitination of CTNNB1 and promotes Wnt signaling (By similarity).By similarity1 Publication

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri514 – 553RING-typePROSITE-ProRule annotationAdd BLAST40

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • ubiquitin protein ligase activity Source: MGI
  • ubiquitin-protein transferase activity Source: UniProtKB

GO - Biological processi

  • positive regulation of canonical Wnt signaling pathway Source: UniProtKB
  • protein autoubiquitination Source: UniProtKB
  • protein ubiquitination Source: UniProtKB

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

BRENDAi2.3.2.B10 3474
ReactomeiR-MMU-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase Rnf220 (EC:2.3.2.27)
Alternative name(s):
RING finger protein 220
RING-type E3 ubiquitin transferase Rnf220Curated
Gene namesi
Name:Rnf220
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1913993 Rnf220

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002776591 – 566E3 ubiquitin-protein ligase Rnf220Add BLAST566

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki277Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei390PhosphoserineCombined sources1

Post-translational modificationi

Auto-ubiquitinated; leads to proteasomal degradation.By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ6PDX6
PeptideAtlasiQ6PDX6
PRIDEiQ6PDX6

PTM databases

iPTMnetiQ6PDX6
PhosphoSitePlusiQ6PDX6

Expressioni

Gene expression databases

BgeeiENSMUSG00000028677
ExpressionAtlasiQ6PDX6 baseline and differential
GenevisibleiQ6PDX6 MM

Interactioni

Subunit structurei

Interacts with SIN3B (PubMed:20170641). Interacts with CTNNB1 (via Armadillo repeats 2-8) (By similarity). Interacts with USP7 (via MATH domain) (By similarity).By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Sin3bQ621415EBI-2795840,EBI-591450

Protein-protein interaction databases

BioGridi211685, 2 interactors
IntActiQ6PDX6, 6 interactors
STRINGi10090.ENSMUSP00000030439

Structurei

3D structure databases

ProteinModelPortaliQ6PDX6
SMRiQ6PDX6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni514 – 522Required for targeting to the cytoplasm9

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili485 – 513Sequence analysisAdd BLAST29

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri514 – 553RING-typePROSITE-ProRule annotationAdd BLAST40

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiENOG410IEJC Eukaryota
ENOG4111JY9 LUCA
GeneTreeiENSGT00390000016573
HOGENOMiHOG000231748
HOVERGENiHBG055526
InParanoidiQ6PDX6
OMAiXGAKKLC
OrthoDBiEOG091G0PN3
PhylomeDBiQ6PDX6
TreeFamiTF324716

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR031824 RNF220_mid
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
PfamiView protein in Pfam
PF15926 RNF220, 2 hits
PROSITEiView protein in PROSITE
PS50089 ZF_RING_2, 1 hit

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6PDX6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDLHRAAFKM ENSSYLPNPL ASPALMVLAS TAEASRDASI PCQQPRPFGV
60 70 80 90 100
PVSVDKDVHI PFTNGSYTFA SMYHRQGGVP GTFANRDFPP SLLHLHPQFA
110 120 130 140 150
PPNLDCTPIS MLNHSGVGAF RPFASTEDRE SYQSAFTPAK RLKNCHDTES
160 170 180 190 200
PHLRFSDADG KEYDFGTQLP SSSPGSLKVD DTGKKIFAVS GLISDRETSS
210 220 230 240 250
SPEDRNDRCK KKAVALFDSQ APLCPICQVL LRPSELQEHM EQELEQLAQL
260 270 280 290 300
PASKNSLLKD AMAPGTPKSL LLSASIKREG DSPTASPHSS ATEDLHHSDR
310 320 330 340 350
YQTFLRVRAN RQTRLNARIG KMKRRKQDEG QREGSCMAED DAVDIEHADS
360 370 380 390 400
NRFEEYEWCG QKRIRATTLL EGGFRGSGFV MCSGKENPDS DADLDVDGDD
410 420 430 440 450
TLEYGKPQYT EADVIPCTGE EPGEAKEREA LRGAVLNGGP PSTRITPEFS
460 470 480 490 500
KWASDEMPST SNGEGSKQEA MQKTCKNSDI EKITEESAVT TFEALKARVR
510 520 530 540 550
ELERQLSRGD RYKCLICMDS YSMPLTSIQC WHVHCEECWL RTLGAKKLCP
560
QCNTITAPGD LRRIYL
Length:566
Mass (Da):62,693
Last modified:July 5, 2004 - v1
Checksum:iAC8D8B716E9E6BAA
GO
Isoform 2 (identifier: Q6PDX6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-284: Missing.
     285-302: ASPHSSATEDLHHSDRYQ → MPRARSGRRSRGADGREE
     331-331: Q → QVCPLCSRPLAGSEQEMSRHVEHCLAK

Show »
Length:308
Mass (Da):34,626
Checksum:iB98AFAB128F4439B
GO
Isoform 3 (identifier: Q6PDX6-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     211-239: KKAVALFDSQAPLCPICQVLLRPSELQEH → YFGWNPLLTPSKPASQKCSTGWQHRTCSQ
     240-566: Missing.

Note: No experimental confirmation available.
Show »
Length:239
Mass (Da):26,252
Checksum:i07FEA45670F940BA
GO
Isoform 4 (identifier: Q6PDX6-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-321: Missing.

Show »
Length:245
Mass (Da):27,484
Checksum:i516B7FB12B22F7BE
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti553N → S in AAH34875 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0230681 – 321Missing in isoform 4. 1 PublicationAdd BLAST321
Alternative sequenceiVSP_0230691 – 284Missing in isoform 2. 1 PublicationAdd BLAST284
Alternative sequenceiVSP_023070211 – 239KKAVA…ELQEH → YFGWNPLLTPSKPASQKCST GWQHRTCSQ in isoform 3. 1 PublicationAdd BLAST29
Alternative sequenceiVSP_023071240 – 566Missing in isoform 3. 1 PublicationAdd BLAST327
Alternative sequenceiVSP_023072285 – 302ASPHS…SDRYQ → MPRARSGRRSRGADGREE in isoform 2. 1 PublicationAdd BLAST18
Alternative sequenceiVSP_023073331Q → QVCPLCSRPLAGSEQEMSRH VEHCLAK in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK041785 mRNA Translation: BAE20604.1
AK172415 mRNA Translation: BAE42996.1
AL645740 Genomic DNA No translation available.
AL844169 Genomic DNA No translation available.
BC034875 mRNA Translation: AAH34875.1
BC056359 mRNA Translation: AAH56359.1
BC058415 mRNA Translation: AAH58415.1
CCDSiCCDS18534.1 [Q6PDX6-1]
CCDS80149.1 [Q6PDX6-4]
RefSeqiNP_001297658.1, NM_001310729.1
NP_001297659.1, NM_001310730.1 [Q6PDX6-2]
NP_001297660.1, NM_001310731.1 [Q6PDX6-4]
NP_080015.3, NM_025739.3 [Q6PDX6-1]
XP_011238891.1, XM_011240589.2 [Q6PDX6-4]
XP_017175844.1, XM_017320355.1 [Q6PDX6-1]
XP_017175846.1, XM_017320357.1 [Q6PDX6-4]
UniGeneiMm.318331
Mm.470234

Genome annotation databases

EnsembliENSMUST00000030439; ENSMUSP00000030439; ENSMUSG00000028677 [Q6PDX6-1]
ENSMUST00000102690; ENSMUSP00000099751; ENSMUSG00000028677 [Q6PDX6-4]
ENSMUST00000221654; ENSMUSP00000152367; ENSMUSG00000028677 [Q6PDX6-2]
GeneIDi66743
KEGGimmu:66743
UCSCiuc008uim.1 mouse [Q6PDX6-4]
uc008uin.1 mouse [Q6PDX6-2]
uc008uip.1 mouse [Q6PDX6-1]
uc008uiq.1 mouse [Q6PDX6-3]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiRN220_MOUSE
AccessioniPrimary (citable) accession number: Q6PDX6
Secondary accession number(s): A2APP0
, Q3T9M4, Q3V3D7, Q8JZY5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: July 5, 2004
Last modified: May 23, 2018
This is version 125 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health