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Protein

Male-specific lethal 1 homolog

Gene

Msl1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of histone acetyltransferase complex responsible for the majority of histone H4 acetylation at 'Lys-17' which is implicated in the formation of higher-order chromatin. structure (By similarity). Greatly enhances MSL2 E3 ubiquitin ligase activity, promoting monoubiquitination of histone H2B at 'Lys-35' (H2BK34Ub). This modification in turn stimulates histone H3 methylation at 'Lys-5' (H3K4me) and 'Lys-80' (H3K79me) and leads to gene activation, including that of HOXA9 and MEIS1. In the MSL complex, acts as a scaffold to tether MSL3 and KAT8 together for enzymatic activity regulation.By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator

Enzyme and pathway databases

ReactomeiR-MMU-3214847. HATs acetylate histones.

Names & Taxonomyi

Protein namesi
Recommended name:
Male-specific lethal 1 homolog
Short name:
MSL-1
Alternative name(s):
Hampin
Male-specific lethal 1-like 1
Short name:
MSL1-like 1
Male-specific lethal-1 homolog 1
Gene namesi
Name:Msl1
Synonyms:Msl1l1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1921276. Msl1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi498 – 4981E → R: Abolishes interaction with KAT8. 1 Publication
Mutagenesisi505 – 5051F → R: Abolishes interaction with KAT8. 1 Publication
Mutagenesisi509 – 5091H → R: Abolishes interaction with KAT8. 1 Publication
Mutagenesisi556 – 5561F → E: Strongly reduces interaction with MSL3; when associated with E-576 and E-589 or E-577 and E-589.
Mutagenesisi576 – 5761A → E: No effect on interaction with MSL3. Reduces interaction; when associated with E-589. Strongly reduces interaction with MSL3; when associated with E-556 and E-589. 1 Publication
Mutagenesisi577 – 5771F → E: No effect on interaction with MSL3. Reduces interaction; when associated with E-589. Strongly reduces interaction with MSL3; when associated with E-556 and E-589. 1 Publication
Mutagenesisi589 – 5891F → E: Strongly reduces interaction with MSL3; when associated with E-556 and E-576 or E-556 and E-577. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 616616Male-specific lethal 1 homologPRO_0000349237Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei66 – 661PhosphoserineBy similarity
Modified residuei127 – 1271PhosphoserineBy similarity
Modified residuei207 – 2071PhosphoserineCombined sources
Modified residuei355 – 3551N6-acetyllysineBy similarity
Modified residuei395 – 3951PhosphoserineBy similarity
Modified residuei398 – 3981PhosphothreonineBy similarity
Modified residuei444 – 4441PhosphoserineBy similarity

Post-translational modificationi

Sumoylated with SUMO1.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ6PDM1.
PaxDbiQ6PDM1.
PeptideAtlasiQ6PDM1.
PRIDEiQ6PDM1.

PTM databases

iPTMnetiQ6PDM1.
PhosphoSiteiQ6PDM1.

Expressioni

Tissue specificityi

Expressed in testis and several other tissues.1 Publication

Gene expression databases

BgeeiENSMUSG00000052915.
ExpressionAtlasiQ6PDM1. baseline and differential.
GenevisibleiQ6PDM1. MM.

Interactioni

Subunit structurei

Component of a multisubunit histone acetyltransferase complex (MSL) at least composed of the KAT8/MOF/MYST1, MSL1/hampin, MSL2 and MSL3. Directly interacts with MSL2 via its coiled coil domain (By similarity). Directly interacts with NUPR1 (By similarity). Interacts with TP53BP1; this interaction may be required for MSL1 DNA repair activity, but not for histone acetyltransferase activity (By similarity). Forms a MSL heterotetrameric core with MSL2 (By similarity). Interacts with KAT8 and MSL3; both interactions are direct.By similarity1 Publication

Protein-protein interaction databases

BioGridi216434. 5 interactions.
IntActiQ6PDM1. 3 interactions.
STRINGi10090.ENSMUSP00000042792.

Structurei

Secondary structure

1
616
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi502 – 53433Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2Y0MX-ray2.70B470-540[»]
2Y0NX-ray3.00E/F/G/H545-597[»]
ProteinModelPortaliQ6PDM1.
SMRiQ6PDM1. Positions 216-266, 495-536, 551-594.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ6PDM1.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni225 – 23915Interaction with MSL2By similarityAdd
BLAST
Regioni500 – 52021Interaction with KAT8Add
BLAST
Regioni552 – 59342Interaction with MSL3 MRG domainAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili215 – 28470By similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi55 – 187133Pro-richAdd
BLAST
Compositional biasi210 – 2167Poly-Gly

Domaini

The coiled coil is formed by helices from two subunits in the MSL1 homodimer.By similarity

Sequence similaritiesi

Belongs to the msl-1 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IDXY. Eukaryota.
ENOG410XPFF. LUCA.
GeneTreeiENSGT00390000018292.
HOVERGENiHBG060802.
InParanoidiQ6PDM1.
KOiK13163.
OMAiHKRKTPF.
OrthoDBiEOG091G07CG.
PhylomeDBiQ6PDM1.
TreeFamiTF330735.

Family and domain databases

InterProiIPR026711. Msl-1.
IPR031840. MSL1_dimer.
IPR029332. PEHE_dom.
[Graphical view]
PANTHERiPTHR21656. PTHR21656. 1 hit.
PfamiPF16801. MSL1_dimer. 1 hit.
PF15275. PEHE. 1 hit.
[Graphical view]
SMARTiSM01300. PEHE. 1 hit.
[Graphical view]

Sequences (6)i

Sequence statusi: Complete.

This entry describes 6 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6PDM1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTMRSAVFKA AAAPAGGNPE QRLDYERAAA LGGPEDESGA AEAHFLPRHR
60 70 80 90 100
KLKEPGPPLA SSQGGSPSPS PAGCGGGKGR GLLLPAGAAP GQQEESWGGS
110 120 130 140 150
VPLPCPPPAT KQAGIGGEPV AAGAGCSPRP KYQAVLPIQT GSIVVAAAKE
160 170 180 190 200
PTPWAGDKGG AAPPAATASD PAGPPPLPLP GPPPLAPTAT AGTLAASEGR
210 220 230 240 250
WKSIRKSPLG GGGGSGASSQ AACLKQILLL QLDLIEQQQQ QLQAKEKEIE
260 270 280 290 300
ELKSERDTLL ARIERMERRM QLVKRDNEKE RHKLLQGYEP EEREEAELSE
310 320 330 340 350
KIKLERQPEL CETSQALPSK PFSCGRSGKG HKRKTPFGNT ERKTPVKKLA
360 370 380 390 400
PEFSKVKTKT PKHSPIKEEP CGSISETVCK RELRSQETPE KPRSSVDTPP
410 420 430 440 450
RLSTPQKGPS THPKEKAFSS EMEDLPYLST TEMYLCRWHQ PPPSPLPLRE
460 470 480 490 500
SSPKKEETVA RCLMPSSVAG ETSVLAVPSW RDHSVEPLRD PNPSDILENL
510 520 530 540 550
DDSVFSKRHA KLELDEKRRK RWDIQRIREQ RILQRLQLRM YKKKGIQESE
560 570 580 590 600
PEVTSFFPEP DDVESLLITP FLPVVAFGRP LPKLAPQNFE LPWLDERSRC
610
RLEIQKKHTP HRTCRK
Length:616
Mass (Da):67,320
Last modified:July 5, 2004 - v1
Checksum:i2679AF230DBC0A7D
GO
Isoform 2 (identifier: Q6PDM1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     461-477: RCLMPSSVAGETSVLAV → I

Note: No experimental confirmation available.
Show »
Length:600
Mass (Da):65,731
Checksum:i57FF48BFFC755A86
GO
Isoform 3 (identifier: Q6PDM1-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-230: Missing.
     231-258: QLDLIEQQQQQLQAKEKEIEELKSERDT → MLRVFARHGQEALIPSLAAQTTTTNRNK
     461-477: RCLMPSSVAGETSVLAV → I

Note: No experimental confirmation available.
Show »
Length:370
Mass (Da):43,157
Checksum:i33AEF4186B4F06CC
GO
Isoform 4 (identifier: Q6PDM1-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     461-463: RCL → SKA
     464-616: Missing.

Note: No experimental confirmation available.
Show »
Length:463
Mass (Da):49,207
Checksum:iC52E141065B00CF2
GO
Isoform 5 (identifier: Q6PDM1-5) [UniParc]FASTAAdd to basket
Also known as: Hampin E

The sequence of this isoform differs from the canonical sequence as follows:
     1-230: Missing.
     231-258: QLDLIEQQQQQLQAKEKEIEELKSERDT → MLRVFARHGQEALIPSLAAQTTTTNRNK
     461-463: RCL → SKA
     464-616: Missing.

Note: No experimental confirmation available.
Show »
Length:233
Mass (Da):26,633
Checksum:iC22A56BF0EEA593A
GO
Isoform 6 (identifier: Q6PDM1-6) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-265: Missing.
     461-463: RCL → SKA
     464-616: Missing.

Note: No experimental confirmation available.
Show »
Length:198
Mass (Da):22,674
Checksum:iC9154B063512747B
GO

Sequence cautioni

The sequence BAC35489 differs from that shown. Reason: Frameshift at position 597. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti344 – 3441T → N in BAB29369 (PubMed:16141072).Curated
Sequence conflicti388 – 3892TP → PQ in BAB29868 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 265265Missing in isoform 6. 1 PublicationVSP_035238Add
BLAST
Alternative sequencei1 – 230230Missing in isoform 3 and isoform 5. 2 PublicationsVSP_035239Add
BLAST
Alternative sequencei231 – 25828QLDLI…SERDT → MLRVFARHGQEALIPSLAAQ TTTTNRNK in isoform 3 and isoform 5. 2 PublicationsVSP_035240Add
BLAST
Alternative sequencei461 – 47717RCLMP…SVLAV → I in isoform 2 and isoform 3. 1 PublicationVSP_035241Add
BLAST
Alternative sequencei461 – 4633RCL → SKA in isoform 4, isoform 5 and isoform 6. 3 PublicationsVSP_035242
Alternative sequencei464 – 616153Missing in isoform 4, isoform 5 and isoform 6. 3 PublicationsVSP_035243Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ387855 mRNA. Translation: ABD46887.1.
AK014463 mRNA. Translation: BAB29369.1.
AK015496 mRNA. Translation: BAB29868.1.
AK161412 mRNA. Translation: BAE36380.1.
AK053719 mRNA. Translation: BAC35489.1. Frameshift.
AL590963 Genomic DNA. Translation: CAM46183.1.
AL590963 Genomic DNA. Translation: CAM46184.1.
AL590963 Genomic DNA. Translation: CAM46185.1.
AL590963 Genomic DNA. Translation: CAM46186.1.
BC043039 mRNA. Translation: AAH43039.1.
BC055715 mRNA. Translation: AAH55715.1.
BC058629 mRNA. Translation: AAH58629.1.
CCDSiCCDS25364.1. [Q6PDM1-1]
RefSeqiNP_082998.2. NM_028722.2. [Q6PDM1-1]
XP_006534414.1. XM_006534351.2. [Q6PDM1-2]
XP_011247586.1. XM_011249284.1. [Q6PDM1-3]
UniGeneiMm.258352.

Genome annotation databases

EnsembliENSMUST00000037915; ENSMUSP00000042792; ENSMUSG00000052915. [Q6PDM1-1]
ENSMUST00000037930; ENSMUSP00000043328; ENSMUSG00000052915. [Q6PDM1-3]
ENSMUST00000107485; ENSMUSP00000103109; ENSMUSG00000052915. [Q6PDM1-4]
ENSMUST00000107487; ENSMUSP00000103111; ENSMUSG00000052915. [Q6PDM1-2]
GeneIDi74026.
KEGGimmu:74026.
UCSCiuc007lhi.2. mouse. [Q6PDM1-4]
uc007lhj.2. mouse. [Q6PDM1-1]
uc007lhk.2. mouse. [Q6PDM1-2]
uc007lhm.1. mouse. [Q6PDM1-5]
uc007lhn.2. mouse. [Q6PDM1-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ387855 mRNA. Translation: ABD46887.1.
AK014463 mRNA. Translation: BAB29369.1.
AK015496 mRNA. Translation: BAB29868.1.
AK161412 mRNA. Translation: BAE36380.1.
AK053719 mRNA. Translation: BAC35489.1. Frameshift.
AL590963 Genomic DNA. Translation: CAM46183.1.
AL590963 Genomic DNA. Translation: CAM46184.1.
AL590963 Genomic DNA. Translation: CAM46185.1.
AL590963 Genomic DNA. Translation: CAM46186.1.
BC043039 mRNA. Translation: AAH43039.1.
BC055715 mRNA. Translation: AAH55715.1.
BC058629 mRNA. Translation: AAH58629.1.
CCDSiCCDS25364.1. [Q6PDM1-1]
RefSeqiNP_082998.2. NM_028722.2. [Q6PDM1-1]
XP_006534414.1. XM_006534351.2. [Q6PDM1-2]
XP_011247586.1. XM_011249284.1. [Q6PDM1-3]
UniGeneiMm.258352.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2Y0MX-ray2.70B470-540[»]
2Y0NX-ray3.00E/F/G/H545-597[»]
ProteinModelPortaliQ6PDM1.
SMRiQ6PDM1. Positions 216-266, 495-536, 551-594.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi216434. 5 interactions.
IntActiQ6PDM1. 3 interactions.
STRINGi10090.ENSMUSP00000042792.

PTM databases

iPTMnetiQ6PDM1.
PhosphoSiteiQ6PDM1.

Proteomic databases

MaxQBiQ6PDM1.
PaxDbiQ6PDM1.
PeptideAtlasiQ6PDM1.
PRIDEiQ6PDM1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000037915; ENSMUSP00000042792; ENSMUSG00000052915. [Q6PDM1-1]
ENSMUST00000037930; ENSMUSP00000043328; ENSMUSG00000052915. [Q6PDM1-3]
ENSMUST00000107485; ENSMUSP00000103109; ENSMUSG00000052915. [Q6PDM1-4]
ENSMUST00000107487; ENSMUSP00000103111; ENSMUSG00000052915. [Q6PDM1-2]
GeneIDi74026.
KEGGimmu:74026.
UCSCiuc007lhi.2. mouse. [Q6PDM1-4]
uc007lhj.2. mouse. [Q6PDM1-1]
uc007lhk.2. mouse. [Q6PDM1-2]
uc007lhm.1. mouse. [Q6PDM1-5]
uc007lhn.2. mouse. [Q6PDM1-3]

Organism-specific databases

CTDi339287.
MGIiMGI:1921276. Msl1.

Phylogenomic databases

eggNOGiENOG410IDXY. Eukaryota.
ENOG410XPFF. LUCA.
GeneTreeiENSGT00390000018292.
HOVERGENiHBG060802.
InParanoidiQ6PDM1.
KOiK13163.
OMAiHKRKTPF.
OrthoDBiEOG091G07CG.
PhylomeDBiQ6PDM1.
TreeFamiTF330735.

Enzyme and pathway databases

ReactomeiR-MMU-3214847. HATs acetylate histones.

Miscellaneous databases

ChiTaRSiMsl1. mouse.
EvolutionaryTraceiQ6PDM1.
PROiQ6PDM1.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000052915.
ExpressionAtlasiQ6PDM1. baseline and differential.
GenevisibleiQ6PDM1. MM.

Family and domain databases

InterProiIPR026711. Msl-1.
IPR031840. MSL1_dimer.
IPR029332. PEHE_dom.
[Graphical view]
PANTHERiPTHR21656. PTHR21656. 1 hit.
PfamiPF16801. MSL1_dimer. 1 hit.
PF15275. PEHE. 1 hit.
[Graphical view]
SMARTiSM01300. PEHE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMSL1_MOUSE
AccessioniPrimary (citable) accession number: Q6PDM1
Secondary accession number(s): A3KFP2
, A3KFP4, Q27QB3, Q3TTE5, Q80XS0, Q8BPN3, Q9CXF9, Q9D5C9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: July 5, 2004
Last modified: September 7, 2016
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.