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Q6PDI5

- ECM29_MOUSE

UniProt

Q6PDI5 - ECM29_MOUSE

Protein

Proteasome-associated protein ECM29 homolog

Gene

Ecm29

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 91 (01 Oct 2014)
      Sequence version 3 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Adapter/scaffolding protein that binds to the 26S proteasome, motor proteins and other compartment specific proteins. May couple the proteasome to different compartments including endosome, endoplasmic reticulum and centrosome. May play a role in ERAD and other enhanced proteolyis By similarity.By similarity

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Proteasome-associated protein ECM29 homolog
    Short name:
    Ecm29
    Gene namesi
    Name:Ecm29
    Synonyms:Kiaa0368
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 4

    Organism-specific databases

    MGIiMGI:2140220. AI314180.

    Subcellular locationi

    Endoplasmic reticulum By similarity. Endoplasmic reticulum-Golgi intermediate compartment By similarity. Endosome By similarity. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity. Nucleus By similarity. Endosomemultivesicular body By similarity. Cytoplasmic vesicle By similarity

    GO - Cellular componenti

    1. endocytic vesicle Source: UniProtKB
    2. endoplasmic reticulum Source: UniProtKB-SubCell
    3. endoplasmic reticulum-Golgi intermediate compartment Source: UniProtKB-SubCell
    4. microtubule organizing center Source: UniProtKB-SubCell
    5. multivesicular body Source: UniProtKB
    6. nucleus Source: UniProtKB-SubCell
    7. proteasome complex Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Endoplasmic reticulum, Endosome, Nucleus, Proteasome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 18401840Proteasome-associated protein ECM29 homologPRO_0000212560Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei825 – 8251PhosphoserineBy similarity
    Modified residuei831 – 8311PhosphothreonineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ6PDI5.
    PaxDbiQ6PDI5.
    PRIDEiQ6PDI5.

    PTM databases

    PhosphoSiteiQ6PDI5.

    Expressioni

    Tissue specificityi

    Expressed at high levels in brain and testis, at low levels in liver, and at very low levels in heart and kidney (at protein level).1 Publication

    Gene expression databases

    ArrayExpressiQ6PDI5.
    BgeeiQ6PDI5.
    CleanExiMM_AI314180.
    GenevestigatoriQ6PDI5.

    Interactioni

    Subunit structurei

    Non-stoichiometric component of the proteasome; associates with the 26S proteasome. Interacts (via N-terminus) with VPS11, VPS26A, VPS36, RAB11FIP4 and RABEP1. Interacts (via C-terminus) with DCTN1, DCTN2, KIF5B, MYH7, MYH10, MYO10 and ARF6 By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ6PDI5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati28 – 6538HEAT 1Add
    BLAST
    Repeati107 – 14438HEAT 2Add
    BLAST
    Repeati162 – 19938HEAT 3Add
    BLAST
    Repeati327 – 36337HEAT 4Add
    BLAST
    Repeati388 – 42740HEAT 5Add
    BLAST
    Repeati430 – 46738HEAT 6Add
    BLAST
    Repeati470 – 50839HEAT 7Add
    BLAST
    Repeati722 – 76039HEAT 8Add
    BLAST
    Repeati773 – 81038HEAT 9Add
    BLAST
    Repeati830 – 86940HEAT 10Add
    BLAST
    Repeati871 – 90838HEAT 11Add
    BLAST
    Repeati932 – 97039HEAT 12Add
    BLAST
    Repeati976 – 101338HEAT 13Add
    BLAST
    Repeati1014 – 105138HEAT 14Add
    BLAST
    Repeati1113 – 115038HEAT 15Add
    BLAST
    Repeati1153 – 119038HEAT 16Add
    BLAST
    Repeati1195 – 123238HEAT 17Add
    BLAST
    Repeati1244 – 128239HEAT 18Add
    BLAST
    Repeati1286 – 132439HEAT 19Add
    BLAST
    Repeati1349 – 138739HEAT 20Add
    BLAST
    Repeati1391 – 142838HEAT 21Add
    BLAST
    Repeati1518 – 155538HEAT 22Add
    BLAST
    Repeati1559 – 159638HEAT 23Add
    BLAST
    Repeati1659 – 169638HEAT 24Add
    BLAST
    Repeati1780 – 182344HEAT 25Add
    BLAST

    Sequence similaritiesi

    Contains 25 HEAT repeats.Curated

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG250280.
    GeneTreeiENSGT00390000007729.
    HOGENOMiHOG000231642.
    HOVERGENiHBG051438.
    KOiK11886.
    OMAiMYCLLEV.
    OrthoDBiEOG79SDW5.
    TreeFamiTF314213.

    Family and domain databases

    Gene3Di1.25.10.10. 6 hits.
    InterProiIPR011989. ARM-like.
    IPR016024. ARM-type_fold.
    IPR026827. ECM29/GCN1.
    IPR024372. Proteasome_stabil_ECM29.
    [Graphical view]
    PANTHERiPTHR23346. PTHR23346. 1 hit.
    PTHR23346:SF19. PTHR23346:SF19. 1 hit.
    PfamiPF13001. Ecm29. 1 hit.
    [Graphical view]
    SUPFAMiSSF48371. SSF48371. 7 hits.

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q6PDI5-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    METGSDSDQL ERVFLRLGHA ETDEQLQNII SKFLPPVLLK LSSTQEGVRK     50
    KVMELLVHLN KRIKSRPKIQ LPVETLLVQY QDPAAVSFVT NFTIIYVKMG 100
    YPRLPVEKQC ELAPTLLTAM EGKPQPQQDS LMHLLIPTLF HMKYPAESSK 150
    SASPFNLAEK PKTVQLLLDF MLDVLLMPYG YVLNESQSRQ NSSSSSQGSS 200
    SNSGGGSGIP QPPPGMSFYA AKRVIGDNPW TPEQLEQCKL GIVKFIEAEQ 250
    VPELEAVLHL VIASSDTRHS VATAADLELK SKQSLIDWNN PAIINKMYKV 300
    YLGDIPLKTK EGAVLKPELK RDPVSTRVKL KIVPHLLRSR QAAETFPANI 350
    QVVYDGLFGT NTNSKLRTLS LQFVHHICLT CPEIKIKPLG PMLLNGLTKL 400
    INEYKEDPKL LSMAYSAVGK LSSRMPHLFT KDIALVQQLF EALCKEEPET 450
    RLAIQEALSM MVGAYSTLEG AQRTLMEALV ASYLIKPEVQ VRQVAVKFAS 500
    TVFPSDHIPS RYLLLLAAGD PREEVHGEAQ RVLRCLPGRN KKESASKQMP 550
    SFPEMVYYIQ EKASHRMKTP VKYMTGTTVL PFNPAAFGEI VLYLRMCLAH 600
    SAGVVPTSQS LADMQDHAPA IGRYIRALMS SSQATASSSN KSGETNPVQI 650
    YTGLLQQLLA GVGGLPVMYC LLEAVSVYPE KLATKFVDKT EWIKSLMSSS 700
    KEEMRELAAL FYSVVVSTVS GIELKSMIEQ LIKATKDNHS PEVQHGSLLA 750
    LGFTVGRYLA KKRVRMAEQH DLETDADLLP EQEEIIRSAT ETIGSFLDST 800
    SPLLAIAACT ALGEIGRNGP LPIPSEGSGF TKLHLVESLL NRIPSSKETN 850
    KMKERAIQTL GYFPVGDGVF PHQKLLLQGL MDSVEAKQIE LQFTIGEAIT 900
    SAAIGTNSVA ARDAWLVTEE EYIPPAGAKV NDVVPWVLDV ILNKHIISPN 950
    PHVRQAACIW LLSLVRKLST HREVKSHLKE IQSAFVSVLS ENDELSQDVA 1000
    SKGLGLVYEL GNEQDQQELV STLVETLMTG KRVKHEVSGE TVVFQGGGLG 1050
    KTPDGQGLST YKELCSLASD LSQPDLVYKF MNLANHHAMW NSRKGAAFGF 1100
    NVIATRAGEQ LAPFLPQLVP RLYRYQFDPN LGIRQAMTSI WNALVTDKSM 1150
    VDKYLKEILQ DLIKNLTSNM WRVRESSCLA LNDLLRGRPL DDVIDKLPEM 1200
    WETLFRVQDD IKESVRKAAE LALKTLSKVC VKMCDPAKGA AGQRTIAVLL 1250
    PCLLDKGMMS PVTEVRALSI NTLVKISKSA GAMLKPHAPK LIPALLESLS 1300
    VLEPQVLNYL SLRATEQEKD VMDSARLSAA KSSPMMETIN MCLQYLDVSV 1350
    LGELVPRLCE LIRSGVGLGT KGGCASVIVS LTTQCPQDLT PYSGKLMSAL 1400
    LSGLTDRNSV IQKSCAFAMG HLVRTSRDSS TEKLLQKLNG WYMEKDEPVY 1450
    KTSCALTIHA IGRYSPDVLK NHAKEVLPLA FLGMHEIADE EKSEKEECNM 1500
    WTEVWQENVP GSFGGIRLYL QELITITQKA LQSQSWKMKA QGAIAMASIS 1550
    KQTSSLVPPY LGMILSALMQ GLAGRTWAGK EELLKAIACV VTACSTELEK 1600
    SVPNQPTTNE ILQAVLKECC KENLKYKIVA ISCAADVLKA TKEDRFQEFS 1650
    DIVIPLIKKN SLESMGVRTT KAEDENEKER ELQLESLLGA FESLGKAWPR 1700
    NPDTQRCYRQ ELCKLMCERL RLSTWKVQLG VLQSMNAFFQ GLMLLEEEHA 1750
    DPEALAEILL ETCKSITYSL ENKTYSSVRT EALSVVELLL KKLEEAKQWE 1800
    SLTAECRGLL IESLATMETD NRPELQEKAS VLKKTLESLE 1840
    Length:1,840
    Mass (Da):203,703
    Last modified:July 27, 2011 - v3
    Checksum:i7FCB7FFDBFD1539D
    GO
    Isoform 2 (identifier: Q6PDI5-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-7: METGSDS → MYHIDCR

    Show »
    Length:1,840
    Mass (Da):203,915
    Checksum:i447D913AA8B0C19E
    GO

    Sequence cautioni

    The sequence BAC30129.1 differs from that shown. Reason: Intron retention.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti1446 – 14461D → E in AAH25035. (PubMed:15489334)Curated
    Sequence conflicti1446 – 14461D → E in BAC97937. (PubMed:14621295)Curated
    Sequence conflicti1449 – 14491V → F in BAC26926. (PubMed:14621295)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 77METGSDS → MYHIDCR in isoform 2. 1 PublicationVSP_013178

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL805972, AL806535, AL929394 Genomic DNA. Translation: CAM17649.1.
    AL929394, AL805972, AL806535 Genomic DNA. Translation: CAM22037.1.
    AL806535, AL805972, AL929394 Genomic DNA. Translation: CAM24807.1.
    BC025035 mRNA. Translation: AAH25035.1.
    BC058684 mRNA. Translation: AAH58684.1.
    AK030369 mRNA. Translation: BAC26926.1.
    AK038775 mRNA. Translation: BAC30129.1. Sequence problems.
    AK129127 mRNA. Translation: BAC97937.1.
    CCDSiCCDS18214.1. [Q6PDI5-1]
    RefSeqiNP_759013.2. NM_172381.2. [Q6PDI5-1]
    XP_006537911.1. XM_006537848.1. [Q6PDI5-2]
    XP_006537912.1. XM_006537849.1. [Q6PDI5-2]
    UniGeneiMm.234801.

    Genome annotation databases

    EnsembliENSMUST00000102889; ENSMUSP00000099953; ENSMUSG00000050812. [Q6PDI5-1]
    GeneIDi230249.
    KEGGimmu:230249.
    UCSCiuc008sze.1. mouse. [Q6PDI5-1]
    uc008szf.1. mouse. [Q6PDI5-2]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL805972 , AL806535 , AL929394 Genomic DNA. Translation: CAM17649.1 .
    AL929394 , AL805972 , AL806535 Genomic DNA. Translation: CAM22037.1 .
    AL806535 , AL805972 , AL929394 Genomic DNA. Translation: CAM24807.1 .
    BC025035 mRNA. Translation: AAH25035.1 .
    BC058684 mRNA. Translation: AAH58684.1 .
    AK030369 mRNA. Translation: BAC26926.1 .
    AK038775 mRNA. Translation: BAC30129.1 . Sequence problems.
    AK129127 mRNA. Translation: BAC97937.1 .
    CCDSi CCDS18214.1. [Q6PDI5-1 ]
    RefSeqi NP_759013.2. NM_172381.2. [Q6PDI5-1 ]
    XP_006537911.1. XM_006537848.1. [Q6PDI5-2 ]
    XP_006537912.1. XM_006537849.1. [Q6PDI5-2 ]
    UniGenei Mm.234801.

    3D structure databases

    ProteinModelPortali Q6PDI5.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q6PDI5.

    Proteomic databases

    MaxQBi Q6PDI5.
    PaxDbi Q6PDI5.
    PRIDEi Q6PDI5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000102889 ; ENSMUSP00000099953 ; ENSMUSG00000050812 . [Q6PDI5-1 ]
    GeneIDi 230249.
    KEGGi mmu:230249.
    UCSCi uc008sze.1. mouse. [Q6PDI5-1 ]
    uc008szf.1. mouse. [Q6PDI5-2 ]

    Organism-specific databases

    MGIi MGI:2140220. AI314180.
    Rougei Search...

    Phylogenomic databases

    eggNOGi NOG250280.
    GeneTreei ENSGT00390000007729.
    HOGENOMi HOG000231642.
    HOVERGENi HBG051438.
    KOi K11886.
    OMAi MYCLLEV.
    OrthoDBi EOG79SDW5.
    TreeFami TF314213.

    Miscellaneous databases

    NextBioi 379853.
    PROi Q6PDI5.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q6PDI5.
    Bgeei Q6PDI5.
    CleanExi MM_AI314180.
    Genevestigatori Q6PDI5.

    Family and domain databases

    Gene3Di 1.25.10.10. 6 hits.
    InterProi IPR011989. ARM-like.
    IPR016024. ARM-type_fold.
    IPR026827. ECM29/GCN1.
    IPR024372. Proteasome_stabil_ECM29.
    [Graphical view ]
    PANTHERi PTHR23346. PTHR23346. 1 hit.
    PTHR23346:SF19. PTHR23346:SF19. 1 hit.
    Pfami PF13001. Ecm29. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48371. SSF48371. 7 hits.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: C57BL/6 and FVB/N.
      Tissue: Brain and Mammary tumor.
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-885 (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1449-1840 (ISOFORM 1).
      Strain: C57BL/6J.
      Tissue: Hypothalamus and Pituitary.
    4. "Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
      Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H.
      DNA Res. 10:167-180(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 612-1840.
      Tissue: Embryonic tail.
    5. "Characterization of mammalian Ecm29, a 26 S proteasome-associated protein that localizes to the nucleus and membrane vesicles."
      Gorbea C., Goellner G.M., Teter K., Holmes R.K., Rechsteiner M.
      J. Biol. Chem. 279:54849-54861(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiECM29_MOUSE
    AccessioniPrimary (citable) accession number: Q6PDI5
    Secondary accession number(s): A2ALW0
    , Q6ZQC9, Q8BSW7, Q8CAH0, Q8R3M6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 15, 2005
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 91 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3