Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q6PDI5

- ECM29_MOUSE

UniProt

Q6PDI5 - ECM29_MOUSE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Proteasome-associated protein ECM29 homolog

Gene

Ecm29

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Adapter/scaffolding protein that binds to the 26S proteasome, motor proteins and other compartment specific proteins. May couple the proteasome to different compartments including endosome, endoplasmic reticulum and centrosome. May play a role in ERAD and other enhanced proteolyis (By similarity).By similarity

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome-associated protein ECM29 homolog
Short name:
Ecm29
Gene namesi
Name:Ecm29
Synonyms:Kiaa0368
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 4

Organism-specific databases

MGIiMGI:2140220. AI314180.

Subcellular locationi

Endoplasmic reticulum By similarity. Endoplasmic reticulum-Golgi intermediate compartment By similarity. Endosome By similarity. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity. Nucleus By similarity. Endosomemultivesicular body By similarity. Cytoplasmic vesicle By similarity

GO - Cellular componenti

  1. cytoskeleton Source: UniProtKB-KW
  2. endocytic vesicle Source: UniProtKB
  3. endoplasmic reticulum Source: UniProtKB-KW
  4. multivesicular body Source: UniProtKB
  5. nucleus Source: UniProtKB-KW
  6. proteasome complex Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Endoplasmic reticulum, Endosome, Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 18401840Proteasome-associated protein ECM29 homologPRO_0000212560Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei825 – 8251PhosphoserineBy similarity
Modified residuei831 – 8311PhosphothreonineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ6PDI5.
PaxDbiQ6PDI5.
PRIDEiQ6PDI5.

PTM databases

PhosphoSiteiQ6PDI5.

Expressioni

Tissue specificityi

Expressed at high levels in brain and testis, at low levels in liver, and at very low levels in heart and kidney (at protein level).1 Publication

Gene expression databases

BgeeiQ6PDI5.
CleanExiMM_AI314180.
ExpressionAtlasiQ6PDI5. baseline and differential.
GenevestigatoriQ6PDI5.

Interactioni

Subunit structurei

Non-stoichiometric component of the proteasome; associates with the 26S proteasome. Interacts (via N-terminus) with VPS11, VPS26A, VPS36, RAB11FIP4 and RABEP1. Interacts (via C-terminus) with DCTN1, DCTN2, KIF5B, MYH7, MYH10, MYO10 and ARF6 (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliQ6PDI5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati28 – 6538HEAT 1Add
BLAST
Repeati107 – 14438HEAT 2Add
BLAST
Repeati162 – 19938HEAT 3Add
BLAST
Repeati327 – 36337HEAT 4Add
BLAST
Repeati388 – 42740HEAT 5Add
BLAST
Repeati430 – 46738HEAT 6Add
BLAST
Repeati470 – 50839HEAT 7Add
BLAST
Repeati722 – 76039HEAT 8Add
BLAST
Repeati773 – 81038HEAT 9Add
BLAST
Repeati830 – 86940HEAT 10Add
BLAST
Repeati871 – 90838HEAT 11Add
BLAST
Repeati932 – 97039HEAT 12Add
BLAST
Repeati976 – 101338HEAT 13Add
BLAST
Repeati1014 – 105138HEAT 14Add
BLAST
Repeati1113 – 115038HEAT 15Add
BLAST
Repeati1153 – 119038HEAT 16Add
BLAST
Repeati1195 – 123238HEAT 17Add
BLAST
Repeati1244 – 128239HEAT 18Add
BLAST
Repeati1286 – 132439HEAT 19Add
BLAST
Repeati1349 – 138739HEAT 20Add
BLAST
Repeati1391 – 142838HEAT 21Add
BLAST
Repeati1518 – 155538HEAT 22Add
BLAST
Repeati1559 – 159638HEAT 23Add
BLAST
Repeati1659 – 169638HEAT 24Add
BLAST
Repeati1780 – 182344HEAT 25Add
BLAST

Sequence similaritiesi

Contains 25 HEAT repeats.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG250280.
GeneTreeiENSGT00390000007729.
HOGENOMiHOG000231642.
HOVERGENiHBG051438.
InParanoidiQ6PDI5.
KOiK11886.
OMAiMYCLLEV.
OrthoDBiEOG79SDW5.
TreeFamiTF314213.

Family and domain databases

Gene3Di1.25.10.10. 6 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR026827. ECM29/GCN1.
IPR024372. Proteasome_stabil_ECM29.
[Graphical view]
PANTHERiPTHR23346. PTHR23346. 1 hit.
PTHR23346:SF19. PTHR23346:SF19. 1 hit.
PfamiPF13001. Ecm29. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 7 hits.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q6PDI5-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
METGSDSDQL ERVFLRLGHA ETDEQLQNII SKFLPPVLLK LSSTQEGVRK
60 70 80 90 100
KVMELLVHLN KRIKSRPKIQ LPVETLLVQY QDPAAVSFVT NFTIIYVKMG
110 120 130 140 150
YPRLPVEKQC ELAPTLLTAM EGKPQPQQDS LMHLLIPTLF HMKYPAESSK
160 170 180 190 200
SASPFNLAEK PKTVQLLLDF MLDVLLMPYG YVLNESQSRQ NSSSSSQGSS
210 220 230 240 250
SNSGGGSGIP QPPPGMSFYA AKRVIGDNPW TPEQLEQCKL GIVKFIEAEQ
260 270 280 290 300
VPELEAVLHL VIASSDTRHS VATAADLELK SKQSLIDWNN PAIINKMYKV
310 320 330 340 350
YLGDIPLKTK EGAVLKPELK RDPVSTRVKL KIVPHLLRSR QAAETFPANI
360 370 380 390 400
QVVYDGLFGT NTNSKLRTLS LQFVHHICLT CPEIKIKPLG PMLLNGLTKL
410 420 430 440 450
INEYKEDPKL LSMAYSAVGK LSSRMPHLFT KDIALVQQLF EALCKEEPET
460 470 480 490 500
RLAIQEALSM MVGAYSTLEG AQRTLMEALV ASYLIKPEVQ VRQVAVKFAS
510 520 530 540 550
TVFPSDHIPS RYLLLLAAGD PREEVHGEAQ RVLRCLPGRN KKESASKQMP
560 570 580 590 600
SFPEMVYYIQ EKASHRMKTP VKYMTGTTVL PFNPAAFGEI VLYLRMCLAH
610 620 630 640 650
SAGVVPTSQS LADMQDHAPA IGRYIRALMS SSQATASSSN KSGETNPVQI
660 670 680 690 700
YTGLLQQLLA GVGGLPVMYC LLEAVSVYPE KLATKFVDKT EWIKSLMSSS
710 720 730 740 750
KEEMRELAAL FYSVVVSTVS GIELKSMIEQ LIKATKDNHS PEVQHGSLLA
760 770 780 790 800
LGFTVGRYLA KKRVRMAEQH DLETDADLLP EQEEIIRSAT ETIGSFLDST
810 820 830 840 850
SPLLAIAACT ALGEIGRNGP LPIPSEGSGF TKLHLVESLL NRIPSSKETN
860 870 880 890 900
KMKERAIQTL GYFPVGDGVF PHQKLLLQGL MDSVEAKQIE LQFTIGEAIT
910 920 930 940 950
SAAIGTNSVA ARDAWLVTEE EYIPPAGAKV NDVVPWVLDV ILNKHIISPN
960 970 980 990 1000
PHVRQAACIW LLSLVRKLST HREVKSHLKE IQSAFVSVLS ENDELSQDVA
1010 1020 1030 1040 1050
SKGLGLVYEL GNEQDQQELV STLVETLMTG KRVKHEVSGE TVVFQGGGLG
1060 1070 1080 1090 1100
KTPDGQGLST YKELCSLASD LSQPDLVYKF MNLANHHAMW NSRKGAAFGF
1110 1120 1130 1140 1150
NVIATRAGEQ LAPFLPQLVP RLYRYQFDPN LGIRQAMTSI WNALVTDKSM
1160 1170 1180 1190 1200
VDKYLKEILQ DLIKNLTSNM WRVRESSCLA LNDLLRGRPL DDVIDKLPEM
1210 1220 1230 1240 1250
WETLFRVQDD IKESVRKAAE LALKTLSKVC VKMCDPAKGA AGQRTIAVLL
1260 1270 1280 1290 1300
PCLLDKGMMS PVTEVRALSI NTLVKISKSA GAMLKPHAPK LIPALLESLS
1310 1320 1330 1340 1350
VLEPQVLNYL SLRATEQEKD VMDSARLSAA KSSPMMETIN MCLQYLDVSV
1360 1370 1380 1390 1400
LGELVPRLCE LIRSGVGLGT KGGCASVIVS LTTQCPQDLT PYSGKLMSAL
1410 1420 1430 1440 1450
LSGLTDRNSV IQKSCAFAMG HLVRTSRDSS TEKLLQKLNG WYMEKDEPVY
1460 1470 1480 1490 1500
KTSCALTIHA IGRYSPDVLK NHAKEVLPLA FLGMHEIADE EKSEKEECNM
1510 1520 1530 1540 1550
WTEVWQENVP GSFGGIRLYL QELITITQKA LQSQSWKMKA QGAIAMASIS
1560 1570 1580 1590 1600
KQTSSLVPPY LGMILSALMQ GLAGRTWAGK EELLKAIACV VTACSTELEK
1610 1620 1630 1640 1650
SVPNQPTTNE ILQAVLKECC KENLKYKIVA ISCAADVLKA TKEDRFQEFS
1660 1670 1680 1690 1700
DIVIPLIKKN SLESMGVRTT KAEDENEKER ELQLESLLGA FESLGKAWPR
1710 1720 1730 1740 1750
NPDTQRCYRQ ELCKLMCERL RLSTWKVQLG VLQSMNAFFQ GLMLLEEEHA
1760 1770 1780 1790 1800
DPEALAEILL ETCKSITYSL ENKTYSSVRT EALSVVELLL KKLEEAKQWE
1810 1820 1830 1840
SLTAECRGLL IESLATMETD NRPELQEKAS VLKKTLESLE
Length:1,840
Mass (Da):203,703
Last modified:July 27, 2011 - v3
Checksum:i7FCB7FFDBFD1539D
GO
Isoform 2 (identifier: Q6PDI5-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-7: METGSDS → MYHIDCR

Show »
Length:1,840
Mass (Da):203,915
Checksum:i447D913AA8B0C19E
GO

Sequence cautioni

The sequence BAC30129.1 differs from that shown. Reason: Intron retention.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1446 – 14461D → E in AAH25035. (PubMed:15489334)Curated
Sequence conflicti1446 – 14461D → E in BAC97937. (PubMed:14621295)Curated
Sequence conflicti1449 – 14491V → F in BAC26926. (PubMed:14621295)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 77METGSDS → MYHIDCR in isoform 2. 1 PublicationVSP_013178

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL805972, AL806535, AL929394 Genomic DNA. Translation: CAM17649.1.
AL929394, AL805972, AL806535 Genomic DNA. Translation: CAM22037.1.
AL806535, AL805972, AL929394 Genomic DNA. Translation: CAM24807.1.
BC025035 mRNA. Translation: AAH25035.1.
BC058684 mRNA. Translation: AAH58684.1.
AK030369 mRNA. Translation: BAC26926.1.
AK038775 mRNA. Translation: BAC30129.1. Sequence problems.
AK129127 mRNA. Translation: BAC97937.1.
CCDSiCCDS18214.1. [Q6PDI5-1]
RefSeqiNP_759013.2. NM_172381.2. [Q6PDI5-1]
XP_006537911.1. XM_006537848.1. [Q6PDI5-2]
XP_006537912.1. XM_006537849.1. [Q6PDI5-2]
UniGeneiMm.234801.

Genome annotation databases

EnsembliENSMUST00000102889; ENSMUSP00000099953; ENSMUSG00000050812. [Q6PDI5-1]
GeneIDi230249.
KEGGimmu:230249.
UCSCiuc008sze.1. mouse. [Q6PDI5-1]
uc008szf.1. mouse. [Q6PDI5-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL805972 , AL806535 , AL929394 Genomic DNA. Translation: CAM17649.1 .
AL929394 , AL805972 , AL806535 Genomic DNA. Translation: CAM22037.1 .
AL806535 , AL805972 , AL929394 Genomic DNA. Translation: CAM24807.1 .
BC025035 mRNA. Translation: AAH25035.1 .
BC058684 mRNA. Translation: AAH58684.1 .
AK030369 mRNA. Translation: BAC26926.1 .
AK038775 mRNA. Translation: BAC30129.1 . Sequence problems.
AK129127 mRNA. Translation: BAC97937.1 .
CCDSi CCDS18214.1. [Q6PDI5-1 ]
RefSeqi NP_759013.2. NM_172381.2. [Q6PDI5-1 ]
XP_006537911.1. XM_006537848.1. [Q6PDI5-2 ]
XP_006537912.1. XM_006537849.1. [Q6PDI5-2 ]
UniGenei Mm.234801.

3D structure databases

ProteinModelPortali Q6PDI5.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei Q6PDI5.

Proteomic databases

MaxQBi Q6PDI5.
PaxDbi Q6PDI5.
PRIDEi Q6PDI5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000102889 ; ENSMUSP00000099953 ; ENSMUSG00000050812 . [Q6PDI5-1 ]
GeneIDi 230249.
KEGGi mmu:230249.
UCSCi uc008sze.1. mouse. [Q6PDI5-1 ]
uc008szf.1. mouse. [Q6PDI5-2 ]

Organism-specific databases

MGIi MGI:2140220. AI314180.
Rougei Search...

Phylogenomic databases

eggNOGi NOG250280.
GeneTreei ENSGT00390000007729.
HOGENOMi HOG000231642.
HOVERGENi HBG051438.
InParanoidi Q6PDI5.
KOi K11886.
OMAi MYCLLEV.
OrthoDBi EOG79SDW5.
TreeFami TF314213.

Miscellaneous databases

NextBioi 379853.
PROi Q6PDI5.
SOURCEi Search...

Gene expression databases

Bgeei Q6PDI5.
CleanExi MM_AI314180.
ExpressionAtlasi Q6PDI5. baseline and differential.
Genevestigatori Q6PDI5.

Family and domain databases

Gene3Di 1.25.10.10. 6 hits.
InterProi IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR026827. ECM29/GCN1.
IPR024372. Proteasome_stabil_ECM29.
[Graphical view ]
PANTHERi PTHR23346. PTHR23346. 1 hit.
PTHR23346:SF19. PTHR23346:SF19. 1 hit.
Pfami PF13001. Ecm29. 1 hit.
[Graphical view ]
SUPFAMi SSF48371. SSF48371. 7 hits.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6 and FVB/N.
    Tissue: Brain and Mammary tumor.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-885 (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1449-1840 (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Hypothalamus and Pituitary.
  4. "Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H.
    DNA Res. 10:167-180(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 612-1840.
    Tissue: Embryonic tail.
  5. "Characterization of mammalian Ecm29, a 26 S proteasome-associated protein that localizes to the nucleus and membrane vesicles."
    Gorbea C., Goellner G.M., Teter K., Holmes R.K., Rechsteiner M.
    J. Biol. Chem. 279:54849-54861(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiECM29_MOUSE
AccessioniPrimary (citable) accession number: Q6PDI5
Secondary accession number(s): A2ALW0
, Q6ZQC9, Q8BSW7, Q8CAH0, Q8R3M6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: July 27, 2011
Last modified: October 29, 2014
This is version 92 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3