Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q6PDI5 (ECM29_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Proteasome-associated protein ECM29 homolog

Short name=Ecm29
Gene names
Name:Ecm29
Synonyms:Kiaa0368
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1840 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Adapter/scaffolding protein that binds to the 26S proteasome, motor proteins and other compartment specific proteins. May couple the proteasome to different compartments including endosome, endoplasmic reticulum and centrosome. May play a role in ERAD and other enhanced proteolyis By similarity.

Subunit structure

Non-stoichiometric component of the proteasome; associates with the 26S proteasome. Interacts (via N-terminus) with VPS11, VPS26A, VPS36, RAB11FIP4 and RABEP1. Interacts (via C-terminus) with DCTN1, DCTN2, KIF5B, MYH7, MYH10, MYO10 and ARF6 By similarity.

Subcellular location

Endoplasmic reticulum By similarity. Endoplasmic reticulum-Golgi intermediate compartment By similarity. Endosome By similarity. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity. Nucleus By similarity. Endosomemultivesicular body By similarity. Cytoplasmic vesicle By similarity.

Tissue specificity

Expressed at high levels in brain and testis, at low levels in liver, and at very low levels in heart and kidney (at protein level). Ref.5

Sequence similarities

Contains 25 HEAT repeats.

Sequence caution

The sequence BAC30129.1 differs from that shown. Reason: Intron retention.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q6PDI5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q6PDI5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-7: METGSDS → MYHIDCR

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 18401840Proteasome-associated protein ECM29 homolog
PRO_0000212560

Regions

Repeat28 – 6538HEAT 1
Repeat107 – 14438HEAT 2
Repeat162 – 19938HEAT 3
Repeat327 – 36337HEAT 4
Repeat388 – 42740HEAT 5
Repeat430 – 46738HEAT 6
Repeat470 – 50839HEAT 7
Repeat722 – 76039HEAT 8
Repeat773 – 81038HEAT 9
Repeat830 – 86940HEAT 10
Repeat871 – 90838HEAT 11
Repeat932 – 97039HEAT 12
Repeat976 – 101338HEAT 13
Repeat1014 – 105138HEAT 14
Repeat1113 – 115038HEAT 15
Repeat1153 – 119038HEAT 16
Repeat1195 – 123238HEAT 17
Repeat1244 – 128239HEAT 18
Repeat1286 – 132439HEAT 19
Repeat1349 – 138739HEAT 20
Repeat1391 – 142838HEAT 21
Repeat1518 – 155538HEAT 22
Repeat1559 – 159638HEAT 23
Repeat1659 – 169638HEAT 24
Repeat1780 – 182344HEAT 25

Amino acid modifications

Modified residue8251Phosphoserine By similarity
Modified residue8311Phosphothreonine By similarity

Natural variations

Alternative sequence1 – 77METGSDS → MYHIDCR in isoform 2.
VSP_013178

Experimental info

Sequence conflict14461D → E in AAH25035. Ref.2
Sequence conflict14461D → E in BAC97937. Ref.4
Sequence conflict14491V → F in BAC26926. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 27, 2011. Version 3.
Checksum: 7FCB7FFDBFD1539D

FASTA1,840203,703
        10         20         30         40         50         60 
METGSDSDQL ERVFLRLGHA ETDEQLQNII SKFLPPVLLK LSSTQEGVRK KVMELLVHLN 

        70         80         90        100        110        120 
KRIKSRPKIQ LPVETLLVQY QDPAAVSFVT NFTIIYVKMG YPRLPVEKQC ELAPTLLTAM 

       130        140        150        160        170        180 
EGKPQPQQDS LMHLLIPTLF HMKYPAESSK SASPFNLAEK PKTVQLLLDF MLDVLLMPYG 

       190        200        210        220        230        240 
YVLNESQSRQ NSSSSSQGSS SNSGGGSGIP QPPPGMSFYA AKRVIGDNPW TPEQLEQCKL 

       250        260        270        280        290        300 
GIVKFIEAEQ VPELEAVLHL VIASSDTRHS VATAADLELK SKQSLIDWNN PAIINKMYKV 

       310        320        330        340        350        360 
YLGDIPLKTK EGAVLKPELK RDPVSTRVKL KIVPHLLRSR QAAETFPANI QVVYDGLFGT 

       370        380        390        400        410        420 
NTNSKLRTLS LQFVHHICLT CPEIKIKPLG PMLLNGLTKL INEYKEDPKL LSMAYSAVGK 

       430        440        450        460        470        480 
LSSRMPHLFT KDIALVQQLF EALCKEEPET RLAIQEALSM MVGAYSTLEG AQRTLMEALV 

       490        500        510        520        530        540 
ASYLIKPEVQ VRQVAVKFAS TVFPSDHIPS RYLLLLAAGD PREEVHGEAQ RVLRCLPGRN 

       550        560        570        580        590        600 
KKESASKQMP SFPEMVYYIQ EKASHRMKTP VKYMTGTTVL PFNPAAFGEI VLYLRMCLAH 

       610        620        630        640        650        660 
SAGVVPTSQS LADMQDHAPA IGRYIRALMS SSQATASSSN KSGETNPVQI YTGLLQQLLA 

       670        680        690        700        710        720 
GVGGLPVMYC LLEAVSVYPE KLATKFVDKT EWIKSLMSSS KEEMRELAAL FYSVVVSTVS 

       730        740        750        760        770        780 
GIELKSMIEQ LIKATKDNHS PEVQHGSLLA LGFTVGRYLA KKRVRMAEQH DLETDADLLP 

       790        800        810        820        830        840 
EQEEIIRSAT ETIGSFLDST SPLLAIAACT ALGEIGRNGP LPIPSEGSGF TKLHLVESLL 

       850        860        870        880        890        900 
NRIPSSKETN KMKERAIQTL GYFPVGDGVF PHQKLLLQGL MDSVEAKQIE LQFTIGEAIT 

       910        920        930        940        950        960 
SAAIGTNSVA ARDAWLVTEE EYIPPAGAKV NDVVPWVLDV ILNKHIISPN PHVRQAACIW 

       970        980        990       1000       1010       1020 
LLSLVRKLST HREVKSHLKE IQSAFVSVLS ENDELSQDVA SKGLGLVYEL GNEQDQQELV 

      1030       1040       1050       1060       1070       1080 
STLVETLMTG KRVKHEVSGE TVVFQGGGLG KTPDGQGLST YKELCSLASD LSQPDLVYKF 

      1090       1100       1110       1120       1130       1140 
MNLANHHAMW NSRKGAAFGF NVIATRAGEQ LAPFLPQLVP RLYRYQFDPN LGIRQAMTSI 

      1150       1160       1170       1180       1190       1200 
WNALVTDKSM VDKYLKEILQ DLIKNLTSNM WRVRESSCLA LNDLLRGRPL DDVIDKLPEM 

      1210       1220       1230       1240       1250       1260 
WETLFRVQDD IKESVRKAAE LALKTLSKVC VKMCDPAKGA AGQRTIAVLL PCLLDKGMMS 

      1270       1280       1290       1300       1310       1320 
PVTEVRALSI NTLVKISKSA GAMLKPHAPK LIPALLESLS VLEPQVLNYL SLRATEQEKD 

      1330       1340       1350       1360       1370       1380 
VMDSARLSAA KSSPMMETIN MCLQYLDVSV LGELVPRLCE LIRSGVGLGT KGGCASVIVS 

      1390       1400       1410       1420       1430       1440 
LTTQCPQDLT PYSGKLMSAL LSGLTDRNSV IQKSCAFAMG HLVRTSRDSS TEKLLQKLNG 

      1450       1460       1470       1480       1490       1500 
WYMEKDEPVY KTSCALTIHA IGRYSPDVLK NHAKEVLPLA FLGMHEIADE EKSEKEECNM 

      1510       1520       1530       1540       1550       1560 
WTEVWQENVP GSFGGIRLYL QELITITQKA LQSQSWKMKA QGAIAMASIS KQTSSLVPPY 

      1570       1580       1590       1600       1610       1620 
LGMILSALMQ GLAGRTWAGK EELLKAIACV VTACSTELEK SVPNQPTTNE ILQAVLKECC 

      1630       1640       1650       1660       1670       1680 
KENLKYKIVA ISCAADVLKA TKEDRFQEFS DIVIPLIKKN SLESMGVRTT KAEDENEKER 

      1690       1700       1710       1720       1730       1740 
ELQLESLLGA FESLGKAWPR NPDTQRCYRQ ELCKLMCERL RLSTWKVQLG VLQSMNAFFQ 

      1750       1760       1770       1780       1790       1800 
GLMLLEEEHA DPEALAEILL ETCKSITYSL ENKTYSSVRT EALSVVELLL KKLEEAKQWE 

      1810       1820       1830       1840 
SLTAECRGLL IESLATMETD NRPELQEKAS VLKKTLESLE 

« Hide

Isoform 2 [UniParc].

Checksum: 447D913AA8B0C19E
Show »

FASTA1,840203,915

References

« Hide 'large scale' references
[1]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: C57BL/6 and FVB/N.
Tissue: Brain and Mammary tumor.
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-885 (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1449-1840 (ISOFORM 1).
Strain: C57BL/6J.
Tissue: Hypothalamus and Pituitary.
[4]"Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H.
DNA Res. 10:167-180(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 612-1840.
Tissue: Embryonic tail.
[5]"Characterization of mammalian Ecm29, a 26 S proteasome-associated protein that localizes to the nucleus and membrane vesicles."
Gorbea C., Goellner G.M., Teter K., Holmes R.K., Rechsteiner M.
J. Biol. Chem. 279:54849-54861(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL805972, AL806535, AL929394 Genomic DNA. Translation: CAM17649.1.
AL929394, AL805972, AL806535 Genomic DNA. Translation: CAM22037.1.
AL806535, AL805972, AL929394 Genomic DNA. Translation: CAM24807.1.
BC025035 mRNA. Translation: AAH25035.1.
BC058684 mRNA. Translation: AAH58684.1.
AK030369 mRNA. Translation: BAC26926.1.
AK038775 mRNA. Translation: BAC30129.1. Sequence problems.
AK129127 mRNA. Translation: BAC97937.1.
CCDSCCDS18214.1. [Q6PDI5-1]
RefSeqNP_759013.2. NM_172381.2. [Q6PDI5-1]
XP_006537911.1. XM_006537848.1. [Q6PDI5-2]
XP_006537912.1. XM_006537849.1. [Q6PDI5-2]
UniGeneMm.234801.

3D structure databases

ProteinModelPortalQ6PDI5.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteQ6PDI5.

Proteomic databases

MaxQBQ6PDI5.
PaxDbQ6PDI5.
PRIDEQ6PDI5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000102889; ENSMUSP00000099953; ENSMUSG00000050812. [Q6PDI5-1]
GeneID230249.
KEGGmmu:230249.
UCSCuc008sze.1. mouse. [Q6PDI5-1]
uc008szf.1. mouse. [Q6PDI5-2]

Organism-specific databases

MGIMGI:2140220. AI314180.
RougeSearch...

Phylogenomic databases

eggNOGNOG250280.
GeneTreeENSGT00390000007729.
HOGENOMHOG000231642.
HOVERGENHBG051438.
KOK11886.
OMAMYCLLEV.
OrthoDBEOG79SDW5.
TreeFamTF314213.

Gene expression databases

ArrayExpressQ6PDI5.
BgeeQ6PDI5.
CleanExMM_AI314180.
GenevestigatorQ6PDI5.

Family and domain databases

Gene3D1.25.10.10. 6 hits.
InterProIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR026827. ECM29/GCN1.
IPR024372. Proteasome_stabil_ECM29.
[Graphical view]
PANTHERPTHR23346. PTHR23346. 1 hit.
PTHR23346:SF19. PTHR23346:SF19. 1 hit.
PfamPF13001. Ecm29. 1 hit.
[Graphical view]
SUPFAMSSF48371. SSF48371. 7 hits.
ProtoNetSearch...

Other

NextBio379853.
PROQ6PDI5.
SOURCESearch...

Entry information

Entry nameECM29_MOUSE
AccessionPrimary (citable) accession number: Q6PDI5
Secondary accession number(s): A2ALW0 expand/collapse secondary AC list , Q6ZQC9, Q8BSW7, Q8CAH0, Q8R3M6
Entry history
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 90 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot