SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q6PDI5

- ECM29_MOUSE

UniProt

Q6PDI5 - ECM29_MOUSE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Proteasome-associated protein ECM29 homolog
Gene
Ecm29, Kiaa0368
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Adapter/scaffolding protein that binds to the 26S proteasome, motor proteins and other compartment specific proteins. May couple the proteasome to different compartments including endosome, endoplasmic reticulum and centrosome. May play a role in ERAD and other enhanced proteolyis By similarity.

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome-associated protein ECM29 homolog
Short name:
Ecm29
Gene namesi
Name:Ecm29
Synonyms:Kiaa0368
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 4

Organism-specific databases

MGIiMGI:2140220. AI314180.

Subcellular locationi

Endoplasmic reticulum By similarity. Endoplasmic reticulum-Golgi intermediate compartment By similarity. Endosome By similarity. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity. Nucleus By similarity. Endosomemultivesicular body By similarity. Cytoplasmic vesicle By similarity

GO - Cellular componenti

  1. endocytic vesicle Source: UniProtKB
  2. endoplasmic reticulum Source: UniProtKB-SubCell
  3. endoplasmic reticulum-Golgi intermediate compartment Source: UniProtKB-SubCell
  4. microtubule organizing center Source: UniProtKB-SubCell
  5. multivesicular body Source: UniProtKB
  6. nucleus Source: UniProtKB-SubCell
  7. proteasome complex Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Endoplasmic reticulum, Endosome, Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 18401840Proteasome-associated protein ECM29 homolog
PRO_0000212560Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei825 – 8251Phosphoserine By similarity
Modified residuei831 – 8311Phosphothreonine By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ6PDI5.
PaxDbiQ6PDI5.
PRIDEiQ6PDI5.

PTM databases

PhosphoSiteiQ6PDI5.

Expressioni

Tissue specificityi

Expressed at high levels in brain and testis, at low levels in liver, and at very low levels in heart and kidney (at protein level).1 Publication

Gene expression databases

ArrayExpressiQ6PDI5.
BgeeiQ6PDI5.
CleanExiMM_AI314180.
GenevestigatoriQ6PDI5.

Interactioni

Subunit structurei

Non-stoichiometric component of the proteasome; associates with the 26S proteasome. Interacts (via N-terminus) with VPS11, VPS26A, VPS36, RAB11FIP4 and RABEP1. Interacts (via C-terminus) with DCTN1, DCTN2, KIF5B, MYH7, MYH10, MYO10 and ARF6 By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ6PDI5.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati28 – 6538HEAT 1
Add
BLAST
Repeati107 – 14438HEAT 2
Add
BLAST
Repeati162 – 19938HEAT 3
Add
BLAST
Repeati327 – 36337HEAT 4
Add
BLAST
Repeati388 – 42740HEAT 5
Add
BLAST
Repeati430 – 46738HEAT 6
Add
BLAST
Repeati470 – 50839HEAT 7
Add
BLAST
Repeati722 – 76039HEAT 8
Add
BLAST
Repeati773 – 81038HEAT 9
Add
BLAST
Repeati830 – 86940HEAT 10
Add
BLAST
Repeati871 – 90838HEAT 11
Add
BLAST
Repeati932 – 97039HEAT 12
Add
BLAST
Repeati976 – 101338HEAT 13
Add
BLAST
Repeati1014 – 105138HEAT 14
Add
BLAST
Repeati1113 – 115038HEAT 15
Add
BLAST
Repeati1153 – 119038HEAT 16
Add
BLAST
Repeati1195 – 123238HEAT 17
Add
BLAST
Repeati1244 – 128239HEAT 18
Add
BLAST
Repeati1286 – 132439HEAT 19
Add
BLAST
Repeati1349 – 138739HEAT 20
Add
BLAST
Repeati1391 – 142838HEAT 21
Add
BLAST
Repeati1518 – 155538HEAT 22
Add
BLAST
Repeati1559 – 159638HEAT 23
Add
BLAST
Repeati1659 – 169638HEAT 24
Add
BLAST
Repeati1780 – 182344HEAT 25
Add
BLAST

Sequence similaritiesi

Contains 25 HEAT repeats.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG250280.
GeneTreeiENSGT00390000007729.
HOGENOMiHOG000231642.
HOVERGENiHBG051438.
KOiK11886.
OMAiMYCLLEV.
OrthoDBiEOG79SDW5.
TreeFamiTF314213.

Family and domain databases

Gene3Di1.25.10.10. 6 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR026827. ECM29/GCN1.
IPR024372. Proteasome_stabil_ECM29.
[Graphical view]
PANTHERiPTHR23346. PTHR23346. 1 hit.
PTHR23346:SF19. PTHR23346:SF19. 1 hit.
PfamiPF13001. Ecm29. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 7 hits.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q6PDI5-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

METGSDSDQL ERVFLRLGHA ETDEQLQNII SKFLPPVLLK LSSTQEGVRK     50
KVMELLVHLN KRIKSRPKIQ LPVETLLVQY QDPAAVSFVT NFTIIYVKMG 100
YPRLPVEKQC ELAPTLLTAM EGKPQPQQDS LMHLLIPTLF HMKYPAESSK 150
SASPFNLAEK PKTVQLLLDF MLDVLLMPYG YVLNESQSRQ NSSSSSQGSS 200
SNSGGGSGIP QPPPGMSFYA AKRVIGDNPW TPEQLEQCKL GIVKFIEAEQ 250
VPELEAVLHL VIASSDTRHS VATAADLELK SKQSLIDWNN PAIINKMYKV 300
YLGDIPLKTK EGAVLKPELK RDPVSTRVKL KIVPHLLRSR QAAETFPANI 350
QVVYDGLFGT NTNSKLRTLS LQFVHHICLT CPEIKIKPLG PMLLNGLTKL 400
INEYKEDPKL LSMAYSAVGK LSSRMPHLFT KDIALVQQLF EALCKEEPET 450
RLAIQEALSM MVGAYSTLEG AQRTLMEALV ASYLIKPEVQ VRQVAVKFAS 500
TVFPSDHIPS RYLLLLAAGD PREEVHGEAQ RVLRCLPGRN KKESASKQMP 550
SFPEMVYYIQ EKASHRMKTP VKYMTGTTVL PFNPAAFGEI VLYLRMCLAH 600
SAGVVPTSQS LADMQDHAPA IGRYIRALMS SSQATASSSN KSGETNPVQI 650
YTGLLQQLLA GVGGLPVMYC LLEAVSVYPE KLATKFVDKT EWIKSLMSSS 700
KEEMRELAAL FYSVVVSTVS GIELKSMIEQ LIKATKDNHS PEVQHGSLLA 750
LGFTVGRYLA KKRVRMAEQH DLETDADLLP EQEEIIRSAT ETIGSFLDST 800
SPLLAIAACT ALGEIGRNGP LPIPSEGSGF TKLHLVESLL NRIPSSKETN 850
KMKERAIQTL GYFPVGDGVF PHQKLLLQGL MDSVEAKQIE LQFTIGEAIT 900
SAAIGTNSVA ARDAWLVTEE EYIPPAGAKV NDVVPWVLDV ILNKHIISPN 950
PHVRQAACIW LLSLVRKLST HREVKSHLKE IQSAFVSVLS ENDELSQDVA 1000
SKGLGLVYEL GNEQDQQELV STLVETLMTG KRVKHEVSGE TVVFQGGGLG 1050
KTPDGQGLST YKELCSLASD LSQPDLVYKF MNLANHHAMW NSRKGAAFGF 1100
NVIATRAGEQ LAPFLPQLVP RLYRYQFDPN LGIRQAMTSI WNALVTDKSM 1150
VDKYLKEILQ DLIKNLTSNM WRVRESSCLA LNDLLRGRPL DDVIDKLPEM 1200
WETLFRVQDD IKESVRKAAE LALKTLSKVC VKMCDPAKGA AGQRTIAVLL 1250
PCLLDKGMMS PVTEVRALSI NTLVKISKSA GAMLKPHAPK LIPALLESLS 1300
VLEPQVLNYL SLRATEQEKD VMDSARLSAA KSSPMMETIN MCLQYLDVSV 1350
LGELVPRLCE LIRSGVGLGT KGGCASVIVS LTTQCPQDLT PYSGKLMSAL 1400
LSGLTDRNSV IQKSCAFAMG HLVRTSRDSS TEKLLQKLNG WYMEKDEPVY 1450
KTSCALTIHA IGRYSPDVLK NHAKEVLPLA FLGMHEIADE EKSEKEECNM 1500
WTEVWQENVP GSFGGIRLYL QELITITQKA LQSQSWKMKA QGAIAMASIS 1550
KQTSSLVPPY LGMILSALMQ GLAGRTWAGK EELLKAIACV VTACSTELEK 1600
SVPNQPTTNE ILQAVLKECC KENLKYKIVA ISCAADVLKA TKEDRFQEFS 1650
DIVIPLIKKN SLESMGVRTT KAEDENEKER ELQLESLLGA FESLGKAWPR 1700
NPDTQRCYRQ ELCKLMCERL RLSTWKVQLG VLQSMNAFFQ GLMLLEEEHA 1750
DPEALAEILL ETCKSITYSL ENKTYSSVRT EALSVVELLL KKLEEAKQWE 1800
SLTAECRGLL IESLATMETD NRPELQEKAS VLKKTLESLE 1840
Length:1,840
Mass (Da):203,703
Last modified:July 27, 2011 - v3
Checksum:i7FCB7FFDBFD1539D
GO
Isoform 2 (identifier: Q6PDI5-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-7: METGSDS → MYHIDCR

Show »
Length:1,840
Mass (Da):203,915
Checksum:i447D913AA8B0C19E
GO

Sequence cautioni

The sequence BAC30129.1 differs from that shown. Reason: Intron retention.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 77METGSDS → MYHIDCR in isoform 2.
VSP_013178

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1446 – 14461D → E in AAH25035. 1 Publication
Sequence conflicti1446 – 14461D → E in BAC97937. 1 Publication
Sequence conflicti1449 – 14491V → F in BAC26926. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL805972, AL806535, AL929394 Genomic DNA. Translation: CAM17649.1.
AL929394, AL805972, AL806535 Genomic DNA. Translation: CAM22037.1.
AL806535, AL805972, AL929394 Genomic DNA. Translation: CAM24807.1.
BC025035 mRNA. Translation: AAH25035.1.
BC058684 mRNA. Translation: AAH58684.1.
AK030369 mRNA. Translation: BAC26926.1.
AK038775 mRNA. Translation: BAC30129.1. Sequence problems.
AK129127 mRNA. Translation: BAC97937.1.
CCDSiCCDS18214.1. [Q6PDI5-1]
RefSeqiNP_759013.2. NM_172381.2. [Q6PDI5-1]
XP_006537911.1. XM_006537848.1. [Q6PDI5-2]
XP_006537912.1. XM_006537849.1. [Q6PDI5-2]
UniGeneiMm.234801.

Genome annotation databases

EnsembliENSMUST00000102889; ENSMUSP00000099953; ENSMUSG00000050812. [Q6PDI5-1]
GeneIDi230249.
KEGGimmu:230249.
UCSCiuc008sze.1. mouse. [Q6PDI5-1]
uc008szf.1. mouse. [Q6PDI5-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL805972 , AL806535 , AL929394 Genomic DNA. Translation: CAM17649.1 .
AL929394 , AL805972 , AL806535 Genomic DNA. Translation: CAM22037.1 .
AL806535 , AL805972 , AL929394 Genomic DNA. Translation: CAM24807.1 .
BC025035 mRNA. Translation: AAH25035.1 .
BC058684 mRNA. Translation: AAH58684.1 .
AK030369 mRNA. Translation: BAC26926.1 .
AK038775 mRNA. Translation: BAC30129.1 . Sequence problems.
AK129127 mRNA. Translation: BAC97937.1 .
CCDSi CCDS18214.1. [Q6PDI5-1 ]
RefSeqi NP_759013.2. NM_172381.2. [Q6PDI5-1 ]
XP_006537911.1. XM_006537848.1. [Q6PDI5-2 ]
XP_006537912.1. XM_006537849.1. [Q6PDI5-2 ]
UniGenei Mm.234801.

3D structure databases

ProteinModelPortali Q6PDI5.
ModBasei Search...

PTM databases

PhosphoSitei Q6PDI5.

Proteomic databases

MaxQBi Q6PDI5.
PaxDbi Q6PDI5.
PRIDEi Q6PDI5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000102889 ; ENSMUSP00000099953 ; ENSMUSG00000050812 . [Q6PDI5-1 ]
GeneIDi 230249.
KEGGi mmu:230249.
UCSCi uc008sze.1. mouse. [Q6PDI5-1 ]
uc008szf.1. mouse. [Q6PDI5-2 ]

Organism-specific databases

MGIi MGI:2140220. AI314180.
Rougei Search...

Phylogenomic databases

eggNOGi NOG250280.
GeneTreei ENSGT00390000007729.
HOGENOMi HOG000231642.
HOVERGENi HBG051438.
KOi K11886.
OMAi MYCLLEV.
OrthoDBi EOG79SDW5.
TreeFami TF314213.

Miscellaneous databases

NextBioi 379853.
PROi Q6PDI5.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q6PDI5.
Bgeei Q6PDI5.
CleanExi MM_AI314180.
Genevestigatori Q6PDI5.

Family and domain databases

Gene3Di 1.25.10.10. 6 hits.
InterProi IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR026827. ECM29/GCN1.
IPR024372. Proteasome_stabil_ECM29.
[Graphical view ]
PANTHERi PTHR23346. PTHR23346. 1 hit.
PTHR23346:SF19. PTHR23346:SF19. 1 hit.
Pfami PF13001. Ecm29. 1 hit.
[Graphical view ]
SUPFAMi SSF48371. SSF48371. 7 hits.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6 and FVB/N.
    Tissue: Brain and Mammary tumor.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-885 (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1449-1840 (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Hypothalamus and Pituitary.
  4. "Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H.
    DNA Res. 10:167-180(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 612-1840.
    Tissue: Embryonic tail.
  5. "Characterization of mammalian Ecm29, a 26 S proteasome-associated protein that localizes to the nucleus and membrane vesicles."
    Gorbea C., Goellner G.M., Teter K., Holmes R.K., Rechsteiner M.
    J. Biol. Chem. 279:54849-54861(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiECM29_MOUSE
AccessioniPrimary (citable) accession number: Q6PDI5
Secondary accession number(s): A2ALW0
, Q6ZQC9, Q8BSW7, Q8CAH0, Q8R3M6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 90 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi