Q6PDG8 (MON1A_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Vacuolar fusion protein MON1 homolog A

Gene names

Name:

Mon1a

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	556 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays an important in membrane trafficking through the secretory apparatus. Not involved in endocytic trafficking to lysosomes. Ref.3
Tissue specificity	Widely expressed (at protein level). Ref.3
Polymorphism	The variant Ser-374 is unique to C57BL strains. It may confer the low iron phenotype observed in these strains.
Sequence similarities	Belongs to the MON1/SAND family.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing Polymorphism
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular iron ion homeostasis Inferred from mutant phenotype Ref.3. Source: MGI protein secretion Inferred from mutant phenotype Ref.3. Source: MGI
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q6PDG8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q6PDG8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     461-461: S → R
     462-556: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 556	556	Vacuolar fusion protein MON1 homolog A		PRO_0000285763
Amino acid modifications
	Modified residue	31	1	Phosphoserine By similarity
	Modified residue	56	1	Phosphoserine Ref.5
	Modified residue	57	1	Phosphotyrosine Ref.5
	Modified residue	69	1	Phosphoserine Ref.5
	Modified residue	72	1	Phosphoserine Ref.5
	Modified residue	79	1	Phosphoserine Ref.4 Ref.6
	Modified residue	91	1	Phosphoserine Ref.6
Natural variations
	Alternative sequence	461	1	S → R in isoform 2.		VSP_024913
	Alternative sequence	462 – 556	95	Missing in isoform 2.		VSP_024914
	Natural variant	374	1	N → S in strain: C57BL. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 15, 2008. Version 3. Checksum: 78E1EA166530540F	FASTA	556	62,131
	10 20 30 40 50 60 MAADMQRKRS SECPEGTLAP SNGQSVERAE SPTPGLTQGT EPGAGQEGAM FVHTRSYEDL 70 80 90 100 110 120 TELEDREASG DSPKECVGSP PPLATDMRQI SQDFSELSTQ LTGVARDLQE EMLPGSSEDW 130 140 150 160 170 180 PEPQGAAGRG AATEPSQEGS TEGEEEDATE AWRLHQKHVF VLSEAGKPVY SRYGSEEALS 190 200 210 220 230 240 STMGVMVALV SFLEADKNAI RSIHADGYKV VFVRRSPLVL VAVARTRQSA QELAQELLYI 250 260 270 280 290 300 YYQILSLLTG AQLSHIFQQK QNYDLRRLLS GSERITDNLL QLMARDPSFL MGAARCLPLA 310 320 330 340 350 360 AAVRDTVSAS LQQARARSLV FSILLAHNQL VALVRRKDQF LHPIDLHLLF NLISSSSSFR 370 380 390 400 410 420 EGEAWTPVCL PKFNAAGFFH AHISYLEPDT DLCLLLISTD REDFFAVSDC RRRFQERLRK 430 440 450 460 470 480 RGTHLALREA LRTPYYSVAQ VGIPDLRHFL YKSKSSGLFT SPEIEAPYSS EEEQERLLGL 490 500 510 520 530 540 YQYLHSRAHN ASRPLKTIYY TGPNENLLAW VTGAFELYMC YSPLGTKASA VSAIHKLMRW 550 IRKEEDRLFI LTPLTY
	Isoform 2 [UniParc]. Checksum: CBC21E21542CC3B8	FASTA	461	51,166
	10 20 30 40 50 60 MAADMQRKRS SECPEGTLAP SNGQSVERAE SPTPGLTQGT EPGAGQEGAM FVHTRSYEDL 70 80 90 100 110 120 TELEDREASG DSPKECVGSP PPLATDMRQI SQDFSELSTQ LTGVARDLQE EMLPGSSEDW 130 140 150 160 170 180 PEPQGAAGRG AATEPSQEGS TEGEEEDATE AWRLHQKHVF VLSEAGKPVY SRYGSEEALS 190 200 210 220 230 240 STMGVMVALV SFLEADKNAI RSIHADGYKV VFVRRSPLVL VAVARTRQSA QELAQELLYI 250 260 270 280 290 300 YYQILSLLTG AQLSHIFQQK QNYDLRRLLS GSERITDNLL QLMARDPSFL MGAARCLPLA 310 320 330 340 350 360 AAVRDTVSAS LQQARARSLV FSILLAHNQL VALVRRKDQF LHPIDLHLLF NLISSSSSFR 370 380 390 400 410 420 EGEAWTPVCL PKFNAAGFFH AHISYLEPDT DLCLLLISTD REDFFAVSDC RRRFQERLRK 430 440 450 460 RGTHLALREA LRTPYYSVAQ VGIPDLRHFL YKSKSSGLFT R

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6J and NOD. Tissue: Embryo and Spleen.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: C57BL/6 and FVB/N. Tissue: Embryonic brain and Mammary tumor.
[3]	"Genetic variation in Mon1a affects protein trafficking and modifies macrophage iron loading in mice." Wang F., Paradkar P.N., Custodio A.O., McVey Ward D., Fleming M.D., Campagna D., Roberts K.A., Boyartchuk V., Dietrich W.F., Kaplan J., Andrews N.C. Nat. Genet. 39:1025-1032(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, VARIANT SER-374, TISSUE SPECIFICITY.
[4]	"Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations." Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M. Mol. Cell. Proteomics 6:283-293(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-79, MASS SPECTROMETRY. Tissue: Brain cortex.
[5]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-56; TYR-57; SER-69 AND SER-72, MASS SPECTROMETRY. Tissue: Liver.
[6]	"The phagosomal proteome in interferon-gamma-activated macrophages." Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P. Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-79 AND SER-91, MASS SPECTROMETRY. Tissue: Macrophage.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK013387 mRNA. Translation: BAB28823.1. AK172624 mRNA. Translation: BAE43102.1. BC048852 mRNA. Translation: AAH48852.1. BC058717 mRNA. Translation: AAH58717.1.
IPI	IPI00457680. IPI00850149.
RefSeq	NP_082645.1. NM_028369.3.
UniGene	Mm.38037.
3D structure databases
ProteinModelPortal	Q6PDG8.
ModBase	Search...
PTM databases
PhosphoSite	Q6PDG8.
Proteomic databases
PaxDb	Q6PDG8.
PRIDE	Q6PDG8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000035202; ENSMUSP00000035202; ENSMUSG00000032583.
GeneID	72825.
KEGG	mmu:72825.
UCSC	uc009rng.2. mouse. uc009rnh.2. mouse.
Organism-specific databases
CTD	84315.
MGI	MGI:1920075. Mon1a.
Phylogenomic databases
eggNOG	NOG256927.
GeneTree	ENSGT00390000006665.
HOGENOM	HOG000031337.
HOVERGEN	HBG064381.
InParanoid	Q6PDG8.
OrthoDB	EOG4CZBFS.
Gene expression databases
Bgee	Q6PDG8.
CleanEx	MM_MON1A.
Genevestigator	Q6PDG8.
Family and domain databases
InterPro	IPR004353. Vacuolar_fusion_protein_MON1. [Graphical view]
PANTHER	PTHR13027. PTHR13027. 1 hit.
Pfam	PF03164. Mon1. 1 hit. [Graphical view]
PRINTS	PR01546. YEAST73DUF.
ProtoNet	Search...
Other
NextBio	336995.
SOURCE	Search...

Entry information

Entry name

MON1A_MOUSE

Accession

Primary (citable) accession number: Q6PDG8
Secondary accession number(s): Q80UP6, Q9CYS2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 2007
Last sequence update:	January 15, 2008
Last modified:	April 3, 2013
This is version 54 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program