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Protein

SWI/SNF complex subunit SMARCC2

Gene

Smarcc2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Can stimulate the ATPase activity of the catalytic subunit of these complexes. May be required for CoREST dependent repression of neuronal specific gene promoters in non-neuronal cells. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator

Keywords - Biological processi

Neurogenesis, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-3214858. RMTs methylate histone arginines.

Names & Taxonomyi

Protein namesi
Recommended name:
SWI/SNF complex subunit SMARCC2
Alternative name(s):
BRG1-associated factor 170
Short name:
BAF170
SWI/SNF complex 170 kDa subunit
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 2
Gene namesi
Name:Smarcc2
Synonyms:Baf170
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:1915344. Smarcc2.

Subcellular locationi

GO - Cellular componenti

  • BAF-type complex Source: MGI
  • nBAF complex Source: UniProtKB
  • npBAF complex Source: UniProtKB
  • nuclear chromatin Source: MGI
  • nucleoplasm Source: MGI
  • protein complex Source: MGI
  • SWI/SNF complex Source: MGI
  • transcriptional repressor complex Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12131213SWI/SNF complex subunit SMARCC2PRO_0000197118Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei283 – 2831PhosphoserineCombined sources
Modified residuei286 – 2861PhosphoserineCombined sources
Modified residuei302 – 3021PhosphoserineCombined sources
Modified residuei304 – 3041PhosphoserineCombined sources
Modified residuei306 – 3061PhosphoserineBy similarity
Modified residuei326 – 3261N6-acetyllysineBy similarity
Modified residuei347 – 3471PhosphoserineCombined sources
Modified residuei387 – 3871PhosphoserineCombined sources
Cross-linki566 – 566Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki704 – 704Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei813 – 8131PhosphoserineBy similarity
Cross-linki848 – 848Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ6PDG5.
MaxQBiQ6PDG5.
PaxDbiQ6PDG5.
PRIDEiQ6PDG5.

PTM databases

iPTMnetiQ6PDG5.
PhosphoSiteiQ6PDG5.
SwissPalmiQ6PDG5.

Expressioni

Developmental stagei

Expressed ubiquitously throughout the developing spinal cord, brain and other embryonic tissues at E10.5-E16.5.1 Publication

Gene expression databases

BgeeiQ6PDG5.
ExpressionAtlasiQ6PDG5. baseline and differential.
GenevisibleiQ6PDG5. MM.

Interactioni

Subunit structurei

Component of a number of multiprotein chromatin-remodeling complexes: Swi/Snf-A (BAF), Swi/Snf-B (PBAF), Brm, Brg1(I) and Brg1(II). Each of the complexes contains a catalytic subunit (either SMARCA4 or SMARCA2), and at least SMARCE1, ACTL6A/BAF53A or ACTL6B/BAF53B, SMARCC1 and SMARCB1. Other subunits specific to each of the complexes may also be present. Component of the BAF complex, which includes at least actin (ACTB), ARID1A, ARID1B/BAF250, SMARCA2, SMARCA4/BRG1, ACTL6A/BAF53, ACTL6B/BAF53B, SMARCE1/BAF57, SMARCC1/BAF155, SMARCC2/BAF170, SMARCB1/SNF5/INI1, and one or more of SMARCD1/BAF60A, SMARCD2/BAF60B, or SMARCD3/BAF60C. In muscle cells, the BAF complex also contains DPF3. May also interact with the SIN3A histone deacetylase transcription repressor complex in conjunction with SMARCA2 and SMARCA4. Interacts with SMARD1 (By similarity). Component of neural progenitors-specific chromatin remodeling complex (npBAF complex) composed of at least, ARID1A/BAF250A or ARID1B/BAF250B, SMARCD1/BAF60A, SMARCD3/BAF60C, SMARCA2/BRM/BAF190B, SMARCA4/BRG1/BAF190A, SMARCB1/BAF47, SMARCC1/BAF155, SMARCE1/BAF57, SMARCC2/BAF170, PHF10/BAF45A, ACTL6A/BAF53A and actin. Component of neuron-specific chromatin remodeling complex (nBAF complex) composed of at least, ARID1A/BAF250A or ARID1B/BAF250B, SMARCD1/BAF60A, SMARCD3/BAF60C, SMARCA2/BRM/BAF190B, SMARCA4/BRG1/BAF190A, SMARCB1/BAF47, SMARCC1/BAF155, SMARCE1/BAF57, SMARCC2/BAF170, DPF1/BAF45B, DPF3/BAF45C, ACTL6B/BAF53B and actin.By similarity1 Publication

Protein-protein interaction databases

BioGridi212652. 9 interactions.
DIPiDIP-48884N.
IntActiQ6PDG5. 7 interactions.
MINTiMINT-4437274.
STRINGi10090.ENSMUSP00000100868.

Structurei

3D structure databases

ProteinModelPortaliQ6PDG5.
SMRiQ6PDG5. Positions 589-654.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini424 – 52198SWIRMPROSITE-ProRule annotationAdd
BLAST
Domaini596 – 64752SANTPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili907 – 93428Sequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi186 – 1894Poly-Glu
Compositional biasi747 – 855109Glu-richAdd
BLAST
Compositional biasi861 – 87010Poly-Ala
Compositional biasi956 – 9616Poly-Gln
Compositional biasi962 – 1212251Pro-richAdd
BLAST

Sequence similaritiesi

Belongs to the SMARCC family.Curated
Contains 1 SANT domain.PROSITE-ProRule annotation
Contains 1 SWIRM domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG1279. Eukaryota.
COG5259. LUCA.
GeneTreeiENSGT00390000018166.
HOGENOMiHOG000047736.
HOVERGENiHBG054849.
InParanoidiQ6PDG5.
KOiK11649.
OMAiPNVKYFE.
OrthoDBiEOG7T4MJH.
PhylomeDBiQ6PDG5.
TreeFamiTF314710.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
1.10.10.60. 1 hit.
InterProiIPR001357. BRCT_dom.
IPR000953. Chromo/shadow_dom.
IPR009057. Homeodomain-like.
IPR001005. SANT/Myb.
IPR017884. SANT_dom.
IPR030092. SMARCC2(BAF170).
IPR032451. SMARCC_C.
IPR032450. SMARCC_N.
IPR007526. SWIRM.
IPR032448. SWIRM-assoc.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR12802:SF38. PTHR12802:SF38. 1 hit.
PfamiPF00249. Myb_DNA-binding. 1 hit.
PF04433. SWIRM. 1 hit.
PF16495. SWIRM-assoc_1. 1 hit.
PF16496. SWIRM-assoc_2. 1 hit.
PF16498. SWIRM-assoc_3. 1 hit.
[Graphical view]
SMARTiSM00298. CHROMO. 1 hit.
SM00717. SANT. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 2 hits.
SSF52113. SSF52113. 2 hits.
PROSITEiPS51293. SANT. 1 hit.
PS50934. SWIRM. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6PDG5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAVRKKDGGP NVKYYEAADT VTQFDNVRLW LGKNYKKYIQ AEPPTNKSLS
60 70 80 90 100
SLVVQLLQFQ EEVFGKHVSN APLTKLPIKC FLDFKAGGSL CHILAAAYKF
110 120 130 140 150
KSDQGWRRYD FQNPSRMDRN VEMFMTIEKS LVQNNCLSRP NIFLCPEIEP
160 170 180 190 200
KLLGKLKDIV KRHQGTISED KSNASHVVYP VPGNLEEEEW VRPVMKRDKQ
210 220 230 240 250
VLLHWGYYPD SYDTWIPASE IEASVEDAPT PEKPRKVHAK WILDTDTFNE
260 270 280 290 300
WMNEEDYEVS DDKSPVSRRK KISAKTLTDE VNSPDSDRRD KKGGNYKKRK
310 320 330 340 350
RSPSPSPTPE AKKKNAKKGP STPYTKSKRG HREEEQEDLT KDMDEPSPVP
360 370 380 390 400
NVEEVTLPKT VNTKKDSESA PVKGGTMTDL DEQDDESMET TGKDEDENST
410 420 430 440 450
GNKGEQTKNP DLHEDNVTEQ THHIIIPSYA AWFDYNSVHA IERRALPEFF
460 470 480 490 500
NGKNKSKTPE IYLAYRNFMI DTYRLNPQEY LTSTACRRNL AGDVCAIMRV
510 520 530 540 550
HAFLEQWGLI NYQVDAESRP TPMGPPPTSH FHVLADTPSG LVPLQPKPPQ
560 570 580 590 600
QSSASQQMLN FPEKGKEKPA DMQNFGLRTD MYTKKNVPSK SKAAASATRE
610 620 630 640 650
WTEQETLLLL EALEMYKDDW NKVSEHVGSR TQDECILHFL RLPIEDPYLE
660 670 680 690 700
DSEASLGPLA YQPIPFSQSG NPVMSTVAFL ASVVDPRVAS AAAKSALEEF
710 720 730 740 750
SKMKEEVPTA LVEAHVRKVE EAAKVTGKAD PAFGLESSGI AGTASDEPER
760 770 780 790 800
IEESGTEEAR PEGQAADEKK EPKEPREGGG AVEEEAKEEI SEVPKKDEEK
810 820 830 840 850
GKEGDSEKES EKSDGDPIVD PEKDKEPTEG QEEVLKEVAE PEGERKTKVE
860 870 880 890 900
RDIGEGNLST AAAAALAAAA VKAKHLAAVE ERKIKSLVAL LVETQMKKLE
910 920 930 940 950
IKLRHFEELE TIMDREREAL EYQRQQLLAD RQAFHMEQLK YAEMRARQQH
960 970 980 990 1000
FQQMHQQQQQ QPPTLPPGSQ PIPPTGAAGP PTVHGLAVPP AAVASAPPGS
1010 1020 1030 1040 1050
GAPPGSLGPS EQIGQAGTTA GPQQPQQAGA PQPGAVPPGV PPPGPHGPSP
1060 1070 1080 1090 1100
FPNQPTPPSM MPGAVPGSGH PGVAGNAPLG LPFGMPPPPP AAPSVIPFGS
1110 1120 1130 1140 1150
LADSISINLP PPPNLHGHHH HLPFAPGTIP PPNLPVSMAN PLHPNLPATT
1160 1170 1180 1190 1200
TMPSSLPLGP GLGSAAAQSP AIVAAVQGNL LPSASPLPDP GTPLPPDPTA
1210
PSPGTVTPVP PPQ
Length:1,213
Mass (Da):132,604
Last modified:January 4, 2005 - v2
Checksum:i6239489440320676
GO
Isoform 2 (identifier: Q6PDG5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1075-1188: Missing.

Show »
Length:1,099
Mass (Da):121,372
Checksum:i3DE4278FA2639DC6
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti550 – 5501Missing in AAH62102 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1075 – 1188114Missing in isoform 2. 1 PublicationVSP_012492Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC058720 mRNA. Translation: AAH58720.1.
BC062102 mRNA. Translation: AAH62102.1.
CCDSiCCDS24278.1. [Q6PDG5-2]
CCDS48727.1. [Q6PDG5-1]
RefSeqiNP_001107568.1. NM_001114096.1.
NP_001107569.1. NM_001114097.1. [Q6PDG5-1]
NP_937803.1. NM_198160.2. [Q6PDG5-2]
UniGeneiMm.417338.

Genome annotation databases

EnsembliENSMUST00000026433; ENSMUSP00000026433; ENSMUSG00000025369. [Q6PDG5-2]
ENSMUST00000105235; ENSMUSP00000100868; ENSMUSG00000025369. [Q6PDG5-1]
GeneIDi68094.
KEGGimmu:68094.
UCSCiuc007hmw.2. mouse. [Q6PDG5-2]
uc007hmx.2. mouse. [Q6PDG5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC058720 mRNA. Translation: AAH58720.1.
BC062102 mRNA. Translation: AAH62102.1.
CCDSiCCDS24278.1. [Q6PDG5-2]
CCDS48727.1. [Q6PDG5-1]
RefSeqiNP_001107568.1. NM_001114096.1.
NP_001107569.1. NM_001114097.1. [Q6PDG5-1]
NP_937803.1. NM_198160.2. [Q6PDG5-2]
UniGeneiMm.417338.

3D structure databases

ProteinModelPortaliQ6PDG5.
SMRiQ6PDG5. Positions 589-654.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi212652. 9 interactions.
DIPiDIP-48884N.
IntActiQ6PDG5. 7 interactions.
MINTiMINT-4437274.
STRINGi10090.ENSMUSP00000100868.

PTM databases

iPTMnetiQ6PDG5.
PhosphoSiteiQ6PDG5.
SwissPalmiQ6PDG5.

Proteomic databases

EPDiQ6PDG5.
MaxQBiQ6PDG5.
PaxDbiQ6PDG5.
PRIDEiQ6PDG5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000026433; ENSMUSP00000026433; ENSMUSG00000025369. [Q6PDG5-2]
ENSMUST00000105235; ENSMUSP00000100868; ENSMUSG00000025369. [Q6PDG5-1]
GeneIDi68094.
KEGGimmu:68094.
UCSCiuc007hmw.2. mouse. [Q6PDG5-2]
uc007hmx.2. mouse. [Q6PDG5-1]

Organism-specific databases

CTDi6601.
MGIiMGI:1915344. Smarcc2.

Phylogenomic databases

eggNOGiKOG1279. Eukaryota.
COG5259. LUCA.
GeneTreeiENSGT00390000018166.
HOGENOMiHOG000047736.
HOVERGENiHBG054849.
InParanoidiQ6PDG5.
KOiK11649.
OMAiPNVKYFE.
OrthoDBiEOG7T4MJH.
PhylomeDBiQ6PDG5.
TreeFamiTF314710.

Enzyme and pathway databases

ReactomeiR-MMU-3214858. RMTs methylate histone arginines.

Miscellaneous databases

ChiTaRSiSmarcc2. mouse.
NextBioi326404.
PROiQ6PDG5.
SOURCEiSearch...

Gene expression databases

BgeeiQ6PDG5.
ExpressionAtlasiQ6PDG5. baseline and differential.
GenevisibleiQ6PDG5. MM.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
1.10.10.60. 1 hit.
InterProiIPR001357. BRCT_dom.
IPR000953. Chromo/shadow_dom.
IPR009057. Homeodomain-like.
IPR001005. SANT/Myb.
IPR017884. SANT_dom.
IPR030092. SMARCC2(BAF170).
IPR032451. SMARCC_C.
IPR032450. SMARCC_N.
IPR007526. SWIRM.
IPR032448. SWIRM-assoc.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR12802:SF38. PTHR12802:SF38. 1 hit.
PfamiPF00249. Myb_DNA-binding. 1 hit.
PF04433. SWIRM. 1 hit.
PF16495. SWIRM-assoc_1. 1 hit.
PF16496. SWIRM-assoc_2. 1 hit.
PF16498. SWIRM-assoc_3. 1 hit.
[Graphical view]
SMARTiSM00298. CHROMO. 1 hit.
SM00717. SANT. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 2 hits.
SSF52113. SSF52113. 2 hits.
PROSITEiPS51293. SANT. 1 hit.
PS50934. SWIRM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J.
    Tissue: Brain.
  2. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-347, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic brain.
  3. "Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
    Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
    Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.
  4. "An essential switch in subunit composition of a chromatin remodeling complex during neural development."
    Lessard J., Wu J.I., Ranish J.A., Wan M., Winslow M.M., Staahl B.T., Wu H., Aebersold R., Graef I.A., Crabtree G.R.
    Neuron 55:201-215(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE NBAF AND NPBAF COMPLEXES, IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE NBAF AND NPBAF COMPLEXES, DEVELOPMENTAL STAGE.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-283; SER-286 AND SER-347, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  6. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-283, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-283; SER-286; SER-302; SER-304; SER-347 AND SER-387, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiSMRC2_MOUSE
AccessioniPrimary (citable) accession number: Q6PDG5
Secondary accession number(s): Q6P6P3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: January 4, 2005
Last modified: May 11, 2016
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.