Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Helicase-like transcription factor

Gene

Hltf

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Has both helicase and E3 ubiquitin ligase activities. Possesses intrinsic ATP-dependent nucleosome-remodeling activity. This activity may be required for transcriptional activation or repression of specific target promoters (By similarity). These may include the SERPINE1, to which this protein can bind directly. Plays a role in error-free postreplication repair (PRR) of damaged DNA and maintains genomic stability through acting as a ubiquitin ligase for 'Lys-63'-linked polyubiquitination of chromatin-bound PCNA (By similarity).By similarity2 Publications

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi294 – 3018ATPPROSITE-ProRule annotation
Zinc fingeri754 – 79542RING-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator, Chromatin regulator, Helicase, Hydrolase, Ligase

Keywords - Biological processi

Transcription, Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, DNA-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Helicase-like transcription factor (EC:3.6.4.-, EC:6.3.2.-)
Alternative name(s):
P113
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 3
Sucrose nonfermenting protein 2-like 3
TNF-response element-binding protein
Gene namesi
Name:Hltf
Synonyms:Smarca3, Snf2l3, Zbu1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1196437. Hltf.

Subcellular locationi

  • Cytoplasm By similarity
  • Nucleus 1 Publication
  • Nucleusnucleolus By similarity
  • Nucleusnucleoplasm By similarity

  • Note: Nuclear localization is stimulated by progesterone.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10031003Helicase-like transcription factorPRO_0000056185Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei195 – 1951Phosphotyrosine; by JAK21 Publication
Modified residuei394 – 3941PhosphoserineBy similarity
Modified residuei395 – 3951PhosphoserineBy similarity
Modified residuei397 – 3971PhosphoserineBy similarity
Modified residuei730 – 7301PhosphothreonineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ6PCN7.
MaxQBiQ6PCN7.
PaxDbiQ6PCN7.
PeptideAtlasiQ6PCN7.
PRIDEiQ6PCN7.

PTM databases

iPTMnetiQ6PCN7.
PhosphoSiteiQ6PCN7.

Expressioni

Tissue specificityi

Expressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle.1 Publication

Developmental stagei

Expressed in the heart from 11.5 dpc. Gradually increases in skeletal muscle to 18.5 dpc.1 Publication

Gene expression databases

BgeeiQ6PCN7.
CleanExiMM_HLTF.
ExpressionAtlasiQ6PCN7. baseline and differential.
GenevisibleiQ6PCN7. MM.

Interactioni

Subunit structurei

Interacts with SP1 and SP3 independently of DNA; the interaction with these transcriptional factors may be required for basal transcription of target genes. Interacts with EGR1; the interaction requires prior binding to DNA and represses c-Rel via a DNA looping mechanism. Interacts with GATA4. Interacts with PCNA; the interaction promotes polyubiquitination of PCNA through association with the UBE2B-RAD18 and UBE2V2-UBE2N ubiquitin ligase complexes. Interacts with RAD18, SHPRH, UBE2V2 and UBE2N (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi203335. 3 interactions.
IntActiQ6PCN7. 2 interactions.
STRINGi10090.ENSMUSP00000002502.

Structurei

3D structure databases

ProteinModelPortaliQ6PCN7.
SMRiQ6PCN7. Positions 58-173.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini433 – 600168Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini831 – 990160Helicase C-terminalPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni38 – 287250DNA bindingBy similarityAdd
BLAST
Regioni919 – 100385Interaction with SP1 and SP3By similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi551 – 5544DEGH box

Sequence similaritiesi

Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation
Contains 1 RING-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri754 – 79542RING-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiENOG410INU2. Eukaryota.
ENOG410XQ1S. LUCA.
GeneTreeiENSGT00830000128423.
HOGENOMiHOG000172619.
HOVERGENiHBG079192.
InParanoidiQ6PCN7.
KOiK15711.
OMAiCFQNTEV.
OrthoDBiEOG761BT4.
PhylomeDBiQ6PCN7.
TreeFamiTF332703.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
3.40.50.300. 3 hits.
InterProiIPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR014905. HIRAN.
IPR030065. HLTF.
IPR027417. P-loop_NTPase.
IPR000330. SNF2_N.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PANTHERiPTHR10799:SF762. PTHR10799:SF762. 5 hits.
PfamiPF00271. Helicase_C. 1 hit.
PF08797. HIRAN. 1 hit.
PF00176. SNF2_N. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SM00910. HIRAN. 1 hit.
SM00184. RING. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 4 hits.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6PCN7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSYTFTRGPV WKYSQSVQYG SHENIPRLSY STFLPHFEFQ DIIPPDDFLT
60 70 80 90 100
SDEEQDLVLF GTMRGQVVGL RYYTGVVNNN EMVALQREPN NPYDKNAIKV
110 120 130 140 150
NNVNGNQVGH IKREIAAAVA YIMDNKLAQV EGVVPFGASN TFTMPLYMTF
160 170 180 190 200
WGKEENRNVV LEQLKKHGFK LGPTPKTLGS SLENAWGSGR AGPSYSRPAH
210 220 230 240 250
VAVQMTTDQL KTEFDKLFED LKEDDRTVEM EPAEAIETPL LPHQKQALAW
260 270 280 290 300
MIARENSKEL PPFWEQRNDL YYNTITNFSV KERPENVHGG ILADDMGLGK
310 320 330 340 350
TLTAIAVILT NFDDGRPLLS KRGKKNHPGK EYKDETIKRR GSNMDKKEDG
360 370 380 390 400
HSESSTCGEE PSISGTPEKS SCTLSQLSSV CPKRRKISVQ YIESSDSEEI
410 420 430 440 450
ETSELPQKMK GKLKNVQLNT KSRVKGSSKV KEDSKFALTF FASATQRKML
460 470 480 490 500
KKGMSMMECS EACDTGERTR ATLIICPLSV LSNWIDQFGQ HVKSEVHLNF
510 520 530 540 550
YVYYGPDRIR DSAWLSKQDI ILTTYNILTH DYGTKDDSPL HSIKWLRVIL
560 570 580 590 600
DEGHAIRNPN AQQTKAVLEL EAERRWVLTG TPIQNSLKDL WSLLSFLKLK
610 620 630 640 650
PFIDREWWYR IIQRPVTTGD EGGLRRLQSL IKNITLRRTK TSKIKGKPVL
660 670 680 690 700
ELPERKVFIQ HITLSEEERK IYQSVKNEGK AAIGRYFTEG TVLAHYADVL
710 720 730 740 750
GLLLRLRQIC CHTHLLTNGM SSSGPSRSDT PEELRKMLIE KMKIILSSGS
760 770 780 790 800
DEECAICLDS LTFPVITHCA HVFCKPCICQ VIHSEQPHAK CPLCRNEIHG
810 820 830 840 850
DNLLECPPEE LACDSDKESS MEWKSSSKIN ALMHALIELR TKDPNIKSLV
860 870 880 890 900
VSQFTTFLSL IETPLKASGF VFTRLDGSMA QKKRVESIQR FQNTEAGSPT
910 920 930 940 950
IMLLSLKAGG VGLNLCAASR VFLMDPAWNP AAEDQCFDRC HRLGQKQEVI
960 970 980 990 1000
ITKFIVKDSV EENMLKIQNT KRDLAAGAFG TKKTDANDMK QAKINEIRTL

IDL
Length:1,003
Mass (Da):113,317
Last modified:July 5, 2004 - v1
Checksum:i91F08509ACEA5513
GO

Sequence cautioni

The sequence AAB63915.1 differs from that shown.Intron retention.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti150 – 1501F → Y in AAB64175 (PubMed:9427542).Curated
Sequence conflicti150 – 1501F → Y in AAB63915 (PubMed:9427542).Curated
Sequence conflicti191 – 1911A → R in AAB64175 (PubMed:9427542).Curated
Sequence conflicti191 – 1911A → R in AAB63915 (PubMed:9427542).Curated
Sequence conflicti394 – 3941S → N in AAB64175 (PubMed:9427542).Curated
Sequence conflicti394 – 3941S → N in AAB63915 (PubMed:9427542).Curated
Sequence conflicti648 – 6481P → S in AAB64175 (PubMed:9427542).Curated
Sequence conflicti648 – 6481P → S in AAB63915 (PubMed:9427542).Curated
Sequence conflicti902 – 9054MLLS → STV in AAB64175 (PubMed:9427542).Curated
Sequence conflicti918 – 9181A → R in AAB64175 (PubMed:9427542).Curated
Sequence conflicti980 – 9801G → A in AAB64175 (PubMed:9427542).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF010138 Genomic DNA. Translation: AAB64175.1.
AF010600 mRNA. Translation: AAB63915.1. Sequence problems.
BC039796 mRNA. Translation: AAH39796.1.
BC057116 mRNA. Translation: AAH57116.1.
BC059240 mRNA. Translation: AAH59240.1.
CCDSiCCDS17261.1.
RefSeqiNP_033236.2. NM_009210.3.
XP_006535491.1. XM_006535428.2.
UniGeneiMm.209650.

Genome annotation databases

EnsembliENSMUST00000002502; ENSMUSP00000002502; ENSMUSG00000002428.
GeneIDi20585.
KEGGimmu:20585.
UCSCiuc008osk.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF010138 Genomic DNA. Translation: AAB64175.1.
AF010600 mRNA. Translation: AAB63915.1. Sequence problems.
BC039796 mRNA. Translation: AAH39796.1.
BC057116 mRNA. Translation: AAH57116.1.
BC059240 mRNA. Translation: AAH59240.1.
CCDSiCCDS17261.1.
RefSeqiNP_033236.2. NM_009210.3.
XP_006535491.1. XM_006535428.2.
UniGeneiMm.209650.

3D structure databases

ProteinModelPortaliQ6PCN7.
SMRiQ6PCN7. Positions 58-173.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203335. 3 interactions.
IntActiQ6PCN7. 2 interactions.
STRINGi10090.ENSMUSP00000002502.

PTM databases

iPTMnetiQ6PCN7.
PhosphoSiteiQ6PCN7.

Proteomic databases

EPDiQ6PCN7.
MaxQBiQ6PCN7.
PaxDbiQ6PCN7.
PeptideAtlasiQ6PCN7.
PRIDEiQ6PCN7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000002502; ENSMUSP00000002502; ENSMUSG00000002428.
GeneIDi20585.
KEGGimmu:20585.
UCSCiuc008osk.1. mouse.

Organism-specific databases

CTDi6596.
MGIiMGI:1196437. Hltf.

Phylogenomic databases

eggNOGiENOG410INU2. Eukaryota.
ENOG410XQ1S. LUCA.
GeneTreeiENSGT00830000128423.
HOGENOMiHOG000172619.
HOVERGENiHBG079192.
InParanoidiQ6PCN7.
KOiK15711.
OMAiCFQNTEV.
OrthoDBiEOG761BT4.
PhylomeDBiQ6PCN7.
TreeFamiTF332703.

Enzyme and pathway databases

UniPathwayiUPA00143.

Miscellaneous databases

PROiQ6PCN7.
SOURCEiSearch...

Gene expression databases

BgeeiQ6PCN7.
CleanExiMM_HLTF.
ExpressionAtlasiQ6PCN7. baseline and differential.
GenevisibleiQ6PCN7. MM.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
3.40.50.300. 3 hits.
InterProiIPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR014905. HIRAN.
IPR030065. HLTF.
IPR027417. P-loop_NTPase.
IPR000330. SNF2_N.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PANTHERiPTHR10799:SF762. PTHR10799:SF762. 5 hits.
PfamiPF00271. Helicase_C. 1 hit.
PF08797. HIRAN. 1 hit.
PF00176. SNF2_N. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SM00910. HIRAN. 1 hit.
SM00184. RING. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 4 hits.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and characterization of P113, a mouse SNF2/SWI2-related transcription factor."
    Zhang Q., Ekhterae D., Kim K.-H.
    Gene 202:31-37(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], SUBCELLULAR LOCATION, FUNCTION.
    Tissue: Liver.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Brain and Thymus.
  3. "Developmental regulation of Zbu1, a DNA-binding member of the SWI2/SNF2 family."
    Gong X., Kaushal S., Ceccarelli E., Bogdanova N., Neville C., Nguyen T., Clark H., Khatib Z.A., Valentine M., Look A.T., Rosenthal N.
    Dev. Biol. 183:166-182(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  4. "Prolactin induces Jak2 phosphorylation of RUSHY195."
    Helmer R.A., Dertien J.S., Chilton B.S.
    Mol. Cell. Endocrinol. 338:79-83(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT TYR-195.

Entry informationi

Entry nameiHLTF_MOUSE
AccessioniPrimary (citable) accession number: Q6PCN7
Secondary accession number(s): O35596, O35597, Q80VT6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: July 5, 2004
Last modified: July 6, 2016
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.