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Q6PCL9 (PAPOG_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Poly(A) polymerase gamma

Short name=PAP-gamma
EC=2.7.7.19
Alternative name(s):
Polynucleotide adenylyltransferase gamma
SRP RNA 3'-adenylating enzyme
Gene names
Name:Papolg
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length739 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Responsible for the post-transcriptional adenylation of the 3'-terminal of mRNA precursors and several small RNAs including signal recognition particle (SRP) RNA, nuclear 7SK RNA, U2 small nuclear RNA, and ribosomal 5S RNA By similarity.

Catalytic activity

ATP + RNA(n) = diphosphate + RNA(n+1).

Cofactor

Binds 2 magnesium ions. Also active with manganese By similarity.

Subcellular location

Nucleus By similarity.

Sequence similarities

Belongs to the poly(A) polymerase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 739739Poly(A) polymerase gamma
PRO_0000253025

Regions

Nucleotide binding99 – 1013ATP By similarity
Nucleotide binding112 – 1143ATP By similarity
Nucleotide binding245 – 2462ATP By similarity

Sites

Metal binding1121Magnesium 1; catalytic By similarity
Metal binding1121Magnesium 2; catalytic By similarity
Metal binding1141Magnesium 1; catalytic By similarity
Metal binding1141Magnesium 2; catalytic By similarity
Metal binding1661Magnesium 2; catalytic By similarity
Binding site1081ATP By similarity
Binding site1661ATP By similarity
Binding site2271ATP By similarity
Binding site2361ATP By similarity
Site1521Interaction with RNA By similarity
Site1571Interaction with RNA By similarity
Site3271Interaction with RNA By similarity
Site3981Interaction with RNA By similarity
Site5311Interaction with RNA By similarity

Amino acid modifications

Modified residue21N6-acetyllysine By similarity
Modified residue231Phosphoserine By similarity
Modified residue6511Phosphoserine By similarity
Modified residue6571Phosphothreonine By similarity

Experimental info

Sequence conflict3641P → T in BAC29194. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q6PCL9 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 603F1AB38DF985BE

FASTA73982,957
        10         20         30         40         50         60 
MKEMSANTML DSQRQQKHYG ITSPISLACP KEIDHIYTQK LIDAMKPFGV FEDEEELNHR 

        70         80         90        100        110        120 
LVVLGKLNNL VKEWISDISE SKNLPPSVVA TVGGKIFTFG SYRLGVHTKG ADIDALCVAP 

       130        140        150        160        170        180 
RHVERSDFFQ SFFEKLKHQD GIRNLRAVED AFVPVIKFEF DGIEIDLVFA RLAIQTISDN 

       190        200        210        220        230        240 
LDLRDDSRLR SLDIRCIRSL NGCRVTDEIL HLVPNKETFR LTLRAVKLWA KRRGIYSNML 

       250        260        270        280        290        300 
GFLGGVSWAM LVARTCQLYP NAAASTLVHK FFLVFSKWEW PNPVLLKQPE ESNLNLPVWD 

       310        320        330        340        350        360 
PRVNPSDRYH LMPIITPAYP QQNSTYNVST STRTVMVEEF KQGLAVTDEI LQGKSDWSKL 

       370        380        390        400        410        420 
LEPPNFFQKY RHYIVLTASA STEENHLEWV GLVESKIRVL VGNLERNEFI TLAHVNPQSF 

       430        440        450        460        470        480 
PGNKEHHKAN NYVSMWFLGI IFRRVENAES VNIDLTYDIQ SFTDTVYRQA NNINMLKDGM 

       490        500        510        520        530        540 
KIEATHVKKK QLHHYLPAEI LQKKKKSLSD VSRSSGGLQS KRSSLDSTCL DSSRDTDSGT 

       550        560        570        580        590        600 
PFNSPVSANK PSNPDSPTGE IERSSAEPVA VVVEKLPSVP PAQGLSIPVI GAKVDPTAKA 

       610        620        630        640        650        660 
VSSPAVCTIP TVVGRNVIPR ITTPHNPVQG QPHLNGISNI TKNVTPKRSH SPPTDGTSKR 

       670        680        690        700        710        720 
LKDIEKFIRL ESAFKESRAA EDRKRKPMDS IGGESMPIPT IDTARKKRLP SKELPDSSSP 

       730 
VPANNIRVIK NSIRLTLNR 

« Hide

References

« Hide 'large scale' references
[1]"Comparative analysis of the sequences of the SRP 3'-adenylating enzyme in human, mouse and Xenopus."
Bhattacharya R., Gaikwad A., Reddy R.
Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Cerebellum.
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J and FVB/N.
Tissue: Mammary tumor.
[5]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
[6]"The phagosomal proteome in interferon-gamma-activated macrophages."
Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
DQ103505 mRNA. Translation: AAZ80292.1.
AK035810 mRNA. Translation: BAC29194.1.
AL662810 Genomic DNA. Translation: CAI25660.1.
BC059271 mRNA. Translation: AAH59271.1.
RefSeqNP_766143.2. NM_172555.2.
UniGeneMm.470599.

3D structure databases

ProteinModelPortalQ6PCL9.
SMRQ6PCL9. Positions 19-497.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteQ6PCL9.

Proteomic databases

PaxDbQ6PCL9.
PRIDEQ6PCL9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000020513; ENSMUSP00000020513; ENSMUSG00000020273.
GeneID216578.
KEGGmmu:216578.
UCSCuc007ifp.2. mouse.

Organism-specific databases

CTD64895.
MGIMGI:2442119. Papolg.

Phylogenomic databases

eggNOGCOG5186.
GeneTreeENSGT00390000017928.
HOGENOMHOG000204376.
HOVERGENHBG053502.
InParanoidQ6PCL9.
KOK14376.
OMAGECMPIP.
OrthoDBEOG7XH6PT.
PhylomeDBQ6PCL9.
TreeFamTF300842.

Gene expression databases

ArrayExpressQ6PCL9.
BgeeQ6PCL9.
CleanExMM_PAPOLG.
GenevestigatorQ6PCL9.

Family and domain databases

Gene3D3.30.70.590. 1 hit.
InterProIPR002934. Nucleotidyltransferase.
IPR011068. NuclTrfase_I_C.
IPR007012. PolA_pol_cen_dom.
IPR007010. PolA_pol_RNA-bd_dom.
IPR014492. PolyA_polymerase.
[Graphical view]
PfamPF01909. NTP_transf_2. 1 hit.
PF04928. PAP_central. 1 hit.
PF04926. PAP_RNA-bind. 1 hit.
[Graphical view]
PIRSFPIRSF018425. PolyA_polymerase. 1 hit.
SUPFAMSSF55003. SSF55003. 1 hit.
ProtoNetSearch...

Other

NextBio375224.
PROQ6PCL9.
SOURCESearch...

Entry information

Entry namePAPOG_MOUSE
AccessionPrimary (citable) accession number: Q6PCL9
Secondary accession number(s): Q8BZC9
Entry history
Integrated into UniProtKB/Swiss-Prot: October 17, 2006
Last sequence update: July 5, 2004
Last modified: April 16, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot