Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q6PCL9

- PAPOG_MOUSE

UniProt

Q6PCL9 - PAPOG_MOUSE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Poly(A) polymerase gamma

Gene

Papolg

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Responsible for the post-transcriptional adenylation of the 3'-terminal of mRNA precursors and several small RNAs including signal recognition particle (SRP) RNA, nuclear 7SK RNA, U2 small nuclear RNA, and ribosomal 5S RNA.By similarity

Catalytic activityi

ATP + RNA(n) = diphosphate + RNA(n+1).

Cofactori

Binds 2 magnesium ions. Also active with manganese By similarity.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei108 – 1081ATPBy similarity
Metal bindingi112 – 1121Magnesium 1; catalyticBy similarity
Metal bindingi112 – 1121Magnesium 2; catalyticBy similarity
Metal bindingi114 – 1141Magnesium 1; catalyticBy similarity
Metal bindingi114 – 1141Magnesium 2; catalyticBy similarity
Sitei152 – 1521Interaction with RNABy similarity
Sitei157 – 1571Interaction with RNABy similarity
Metal bindingi166 – 1661Magnesium 2; catalyticBy similarity
Binding sitei166 – 1661ATPBy similarity
Binding sitei227 – 2271ATPBy similarity
Binding sitei236 – 2361ATPBy similarity
Sitei327 – 3271Interaction with RNABy similarity
Sitei398 – 3981Interaction with RNABy similarity
Sitei531 – 5311Interaction with RNABy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi99 – 1013ATPBy similarity
Nucleotide bindingi112 – 1143ATPBy similarity
Nucleotide bindingi245 – 2462ATPBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB
  3. polynucleotide adenylyltransferase activity Source: UniProtKB
  4. RNA binding Source: UniProtKB-KW

GO - Biological processi

  1. mRNA polyadenylation Source: RefGenome
  2. RNA polyadenylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

mRNA processing

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Poly(A) polymerase gamma (EC:2.7.7.19)
Short name:
PAP-gamma
Alternative name(s):
Polynucleotide adenylyltransferase gamma
SRP RNA 3'-adenylating enzyme
Gene namesi
Name:Papolg
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 11

Organism-specific databases

MGIiMGI:2442119. Papolg.

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. cytoplasm Source: Ensembl
  2. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 739739Poly(A) polymerase gammaPRO_0000253025Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N6-acetyllysineBy similarity
Modified residuei23 – 231PhosphoserineBy similarity
Modified residuei651 – 6511PhosphoserineBy similarity
Modified residuei657 – 6571PhosphothreonineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ6PCL9.
PaxDbiQ6PCL9.
PRIDEiQ6PCL9.

PTM databases

PhosphoSiteiQ6PCL9.

Expressioni

Gene expression databases

BgeeiQ6PCL9.
CleanExiMM_PAPOLG.
ExpressionAtlasiQ6PCL9. baseline and differential.
GenevestigatoriQ6PCL9.

Structurei

3D structure databases

ProteinModelPortaliQ6PCL9.
SMRiQ6PCL9. Positions 17-501.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the poly(A) polymerase family.Curated

Phylogenomic databases

eggNOGiCOG5186.
GeneTreeiENSGT00390000017928.
HOGENOMiHOG000204376.
HOVERGENiHBG053502.
InParanoidiQ6PCL9.
KOiK14376.
OMAiGECMPIP.
OrthoDBiEOG7XH6PT.
PhylomeDBiQ6PCL9.
TreeFamiTF300842.

Family and domain databases

Gene3Di3.30.70.590. 1 hit.
InterProiIPR002934. Nucleotidyltransferase.
IPR011068. NuclTrfase_I_C.
IPR007012. PolA_pol_cen_dom.
IPR007010. PolA_pol_RNA-bd_dom.
IPR014492. PolyA_polymerase.
[Graphical view]
PfamiPF01909. NTP_transf_2. 1 hit.
PF04928. PAP_central. 1 hit.
PF04926. PAP_RNA-bind. 1 hit.
[Graphical view]
PIRSFiPIRSF018425. PolyA_polymerase. 1 hit.
SUPFAMiSSF55003. SSF55003. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6PCL9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKEMSANTML DSQRQQKHYG ITSPISLACP KEIDHIYTQK LIDAMKPFGV
60 70 80 90 100
FEDEEELNHR LVVLGKLNNL VKEWISDISE SKNLPPSVVA TVGGKIFTFG
110 120 130 140 150
SYRLGVHTKG ADIDALCVAP RHVERSDFFQ SFFEKLKHQD GIRNLRAVED
160 170 180 190 200
AFVPVIKFEF DGIEIDLVFA RLAIQTISDN LDLRDDSRLR SLDIRCIRSL
210 220 230 240 250
NGCRVTDEIL HLVPNKETFR LTLRAVKLWA KRRGIYSNML GFLGGVSWAM
260 270 280 290 300
LVARTCQLYP NAAASTLVHK FFLVFSKWEW PNPVLLKQPE ESNLNLPVWD
310 320 330 340 350
PRVNPSDRYH LMPIITPAYP QQNSTYNVST STRTVMVEEF KQGLAVTDEI
360 370 380 390 400
LQGKSDWSKL LEPPNFFQKY RHYIVLTASA STEENHLEWV GLVESKIRVL
410 420 430 440 450
VGNLERNEFI TLAHVNPQSF PGNKEHHKAN NYVSMWFLGI IFRRVENAES
460 470 480 490 500
VNIDLTYDIQ SFTDTVYRQA NNINMLKDGM KIEATHVKKK QLHHYLPAEI
510 520 530 540 550
LQKKKKSLSD VSRSSGGLQS KRSSLDSTCL DSSRDTDSGT PFNSPVSANK
560 570 580 590 600
PSNPDSPTGE IERSSAEPVA VVVEKLPSVP PAQGLSIPVI GAKVDPTAKA
610 620 630 640 650
VSSPAVCTIP TVVGRNVIPR ITTPHNPVQG QPHLNGISNI TKNVTPKRSH
660 670 680 690 700
SPPTDGTSKR LKDIEKFIRL ESAFKESRAA EDRKRKPMDS IGGESMPIPT
710 720 730
IDTARKKRLP SKELPDSSSP VPANNIRVIK NSIRLTLNR
Length:739
Mass (Da):82,957
Last modified:July 5, 2004 - v1
Checksum:i603F1AB38DF985BE
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti364 – 3641P → T in BAC29194. (PubMed:16141072)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ103505 mRNA. Translation: AAZ80292.1.
AK035810 mRNA. Translation: BAC29194.1.
AL662810 Genomic DNA. Translation: CAI25660.1.
BC059271 mRNA. Translation: AAH59271.1.
CCDSiCCDS24481.1.
RefSeqiNP_766143.2. NM_172555.2.
UniGeneiMm.470599.

Genome annotation databases

EnsembliENSMUST00000020513; ENSMUSP00000020513; ENSMUSG00000020273.
GeneIDi216578.
KEGGimmu:216578.
UCSCiuc007ifp.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ103505 mRNA. Translation: AAZ80292.1 .
AK035810 mRNA. Translation: BAC29194.1 .
AL662810 Genomic DNA. Translation: CAI25660.1 .
BC059271 mRNA. Translation: AAH59271.1 .
CCDSi CCDS24481.1.
RefSeqi NP_766143.2. NM_172555.2.
UniGenei Mm.470599.

3D structure databases

ProteinModelPortali Q6PCL9.
SMRi Q6PCL9. Positions 17-501.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei Q6PCL9.

Proteomic databases

MaxQBi Q6PCL9.
PaxDbi Q6PCL9.
PRIDEi Q6PCL9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000020513 ; ENSMUSP00000020513 ; ENSMUSG00000020273 .
GeneIDi 216578.
KEGGi mmu:216578.
UCSCi uc007ifp.2. mouse.

Organism-specific databases

CTDi 64895.
MGIi MGI:2442119. Papolg.

Phylogenomic databases

eggNOGi COG5186.
GeneTreei ENSGT00390000017928.
HOGENOMi HOG000204376.
HOVERGENi HBG053502.
InParanoidi Q6PCL9.
KOi K14376.
OMAi GECMPIP.
OrthoDBi EOG7XH6PT.
PhylomeDBi Q6PCL9.
TreeFami TF300842.

Miscellaneous databases

NextBioi 375224.
PROi Q6PCL9.
SOURCEi Search...

Gene expression databases

Bgeei Q6PCL9.
CleanExi MM_PAPOLG.
ExpressionAtlasi Q6PCL9. baseline and differential.
Genevestigatori Q6PCL9.

Family and domain databases

Gene3Di 3.30.70.590. 1 hit.
InterProi IPR002934. Nucleotidyltransferase.
IPR011068. NuclTrfase_I_C.
IPR007012. PolA_pol_cen_dom.
IPR007010. PolA_pol_RNA-bd_dom.
IPR014492. PolyA_polymerase.
[Graphical view ]
Pfami PF01909. NTP_transf_2. 1 hit.
PF04928. PAP_central. 1 hit.
PF04926. PAP_RNA-bind. 1 hit.
[Graphical view ]
PIRSFi PIRSF018425. PolyA_polymerase. 1 hit.
SUPFAMi SSF55003. SSF55003. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Comparative analysis of the sequences of the SRP 3'-adenylating enzyme in human, mouse and Xenopus."
    Bhattacharya R., Gaikwad A., Reddy R.
    Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Cerebellum.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J and FVB/N.
    Tissue: Mammary tumor.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  6. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPAPOG_MOUSE
AccessioniPrimary (citable) accession number: Q6PCL9
Secondary accession number(s): Q8BZC9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 17, 2006
Last sequence update: July 5, 2004
Last modified: October 29, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3