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Protein

Poly(A) polymerase gamma

Gene

Papolg

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Responsible for the post-transcriptional adenylation of the 3'-terminal of mRNA precursors and several small RNAs including signal recognition particle (SRP) RNA, nuclear 7SK RNA, U2 small nuclear RNA, and ribosomal 5S RNA.By similarity

Catalytic activityi

ATP + RNA(n) = diphosphate + RNA(n+1).

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium ions. Also active with manganese.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei108ATPBy similarity1
Metal bindingi112Magnesium 1; catalyticBy similarity1
Metal bindingi112Magnesium 2; catalyticBy similarity1
Metal bindingi114Magnesium 1; catalyticBy similarity1
Metal bindingi114Magnesium 2; catalyticBy similarity1
Metal bindingi166Magnesium 2; catalyticBy similarity1
Binding sitei166ATPBy similarity1
Binding sitei227ATPBy similarity1
Binding sitei236ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi99 – 101ATPBy similarity3
Nucleotide bindingi112 – 114ATPBy similarity3
Nucleotide bindingi245 – 246ATPBy similarity2

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

mRNA processing

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Poly(A) polymerase gamma (EC:2.7.7.19)
Short name:
PAP-gamma
Alternative name(s):
Polynucleotide adenylyltransferase gamma
SRP RNA 3'-adenylating enzyme
Signal recognition particle RNA-adenylating enzyme
Short name:
SRP RNA-adenylating enzyme
Gene namesi
Name:Papolg
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:2442119. Papolg.

Subcellular locationi

  • Nucleus By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002530251 – 739Poly(A) polymerase gammaAdd BLAST739

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N6-acetyllysineBy similarity1
Modified residuei23PhosphoserineBy similarity1
Modified residuei524PhosphoserineBy similarity1
Modified residuei602PhosphoserineBy similarity1
Modified residuei651PhosphoserineBy similarity1
Modified residuei657PhosphothreonineBy similarity1
Modified residuei711PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ6PCL9.
MaxQBiQ6PCL9.
PaxDbiQ6PCL9.
PeptideAtlasiQ6PCL9.
PRIDEiQ6PCL9.

PTM databases

iPTMnetiQ6PCL9.
PhosphoSitePlusiQ6PCL9.

Expressioni

Gene expression databases

BgeeiENSMUSG00000020273.
CleanExiMM_PAPOLG.
ExpressionAtlasiQ6PCL9. baseline and differential.
GenevisibleiQ6PCL9. MM.

Interactioni

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei152Interaction with RNABy similarity1
Sitei157Interaction with RNABy similarity1
Sitei327Interaction with RNABy similarity1
Sitei398Interaction with RNABy similarity1
Sitei531Interaction with RNABy similarity1

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000020513.

Structurei

3D structure databases

ProteinModelPortaliQ6PCL9.
SMRiQ6PCL9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the poly(A) polymerase family.Curated

Phylogenomic databases

eggNOGiKOG2245. Eukaryota.
COG5186. LUCA.
GeneTreeiENSGT00390000017928.
HOGENOMiHOG000204376.
HOVERGENiHBG053502.
InParanoidiQ6PCL9.
KOiK14376.
OMAiMLDSQRQ.
OrthoDBiEOG091G0571.
PhylomeDBiQ6PCL9.
TreeFamiTF300842.

Family and domain databases

Gene3Di3.30.70.590. 1 hit.
InterProiIPR011068. NuclTrfase_I_C.
IPR007012. PolA_pol_cen_dom.
IPR007010. PolA_pol_RNA-bd_dom.
IPR014492. PolyA_polymerase.
IPR002934. Polymerase_NTP_transf_dom.
[Graphical view]
PANTHERiPTHR10682. PTHR10682. 1 hit.
PfamiPF01909. NTP_transf_2. 1 hit.
PF04928. PAP_central. 1 hit.
PF04926. PAP_RNA-bind. 2 hits.
[Graphical view]
SUPFAMiSSF55003. SSF55003. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6PCL9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKEMSANTML DSQRQQKHYG ITSPISLACP KEIDHIYTQK LIDAMKPFGV
60 70 80 90 100
FEDEEELNHR LVVLGKLNNL VKEWISDISE SKNLPPSVVA TVGGKIFTFG
110 120 130 140 150
SYRLGVHTKG ADIDALCVAP RHVERSDFFQ SFFEKLKHQD GIRNLRAVED
160 170 180 190 200
AFVPVIKFEF DGIEIDLVFA RLAIQTISDN LDLRDDSRLR SLDIRCIRSL
210 220 230 240 250
NGCRVTDEIL HLVPNKETFR LTLRAVKLWA KRRGIYSNML GFLGGVSWAM
260 270 280 290 300
LVARTCQLYP NAAASTLVHK FFLVFSKWEW PNPVLLKQPE ESNLNLPVWD
310 320 330 340 350
PRVNPSDRYH LMPIITPAYP QQNSTYNVST STRTVMVEEF KQGLAVTDEI
360 370 380 390 400
LQGKSDWSKL LEPPNFFQKY RHYIVLTASA STEENHLEWV GLVESKIRVL
410 420 430 440 450
VGNLERNEFI TLAHVNPQSF PGNKEHHKAN NYVSMWFLGI IFRRVENAES
460 470 480 490 500
VNIDLTYDIQ SFTDTVYRQA NNINMLKDGM KIEATHVKKK QLHHYLPAEI
510 520 530 540 550
LQKKKKSLSD VSRSSGGLQS KRSSLDSTCL DSSRDTDSGT PFNSPVSANK
560 570 580 590 600
PSNPDSPTGE IERSSAEPVA VVVEKLPSVP PAQGLSIPVI GAKVDPTAKA
610 620 630 640 650
VSSPAVCTIP TVVGRNVIPR ITTPHNPVQG QPHLNGISNI TKNVTPKRSH
660 670 680 690 700
SPPTDGTSKR LKDIEKFIRL ESAFKESRAA EDRKRKPMDS IGGESMPIPT
710 720 730
IDTARKKRLP SKELPDSSSP VPANNIRVIK NSIRLTLNR
Length:739
Mass (Da):82,957
Last modified:July 5, 2004 - v1
Checksum:i603F1AB38DF985BE
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti364P → T in BAC29194 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ103505 mRNA. Translation: AAZ80292.1.
AK035810 mRNA. Translation: BAC29194.1.
AL662810 Genomic DNA. Translation: CAI25660.1.
BC059271 mRNA. Translation: AAH59271.1.
CCDSiCCDS24481.1.
RefSeqiNP_766143.2. NM_172555.2.
UniGeneiMm.470599.

Genome annotation databases

EnsembliENSMUST00000020513; ENSMUSP00000020513; ENSMUSG00000020273.
GeneIDi216578.
KEGGimmu:216578.
UCSCiuc007ifp.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ103505 mRNA. Translation: AAZ80292.1.
AK035810 mRNA. Translation: BAC29194.1.
AL662810 Genomic DNA. Translation: CAI25660.1.
BC059271 mRNA. Translation: AAH59271.1.
CCDSiCCDS24481.1.
RefSeqiNP_766143.2. NM_172555.2.
UniGeneiMm.470599.

3D structure databases

ProteinModelPortaliQ6PCL9.
SMRiQ6PCL9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000020513.

PTM databases

iPTMnetiQ6PCL9.
PhosphoSitePlusiQ6PCL9.

Proteomic databases

EPDiQ6PCL9.
MaxQBiQ6PCL9.
PaxDbiQ6PCL9.
PeptideAtlasiQ6PCL9.
PRIDEiQ6PCL9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000020513; ENSMUSP00000020513; ENSMUSG00000020273.
GeneIDi216578.
KEGGimmu:216578.
UCSCiuc007ifp.2. mouse.

Organism-specific databases

CTDi64895.
MGIiMGI:2442119. Papolg.

Phylogenomic databases

eggNOGiKOG2245. Eukaryota.
COG5186. LUCA.
GeneTreeiENSGT00390000017928.
HOGENOMiHOG000204376.
HOVERGENiHBG053502.
InParanoidiQ6PCL9.
KOiK14376.
OMAiMLDSQRQ.
OrthoDBiEOG091G0571.
PhylomeDBiQ6PCL9.
TreeFamiTF300842.

Miscellaneous databases

PROiQ6PCL9.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000020273.
CleanExiMM_PAPOLG.
ExpressionAtlasiQ6PCL9. baseline and differential.
GenevisibleiQ6PCL9. MM.

Family and domain databases

Gene3Di3.30.70.590. 1 hit.
InterProiIPR011068. NuclTrfase_I_C.
IPR007012. PolA_pol_cen_dom.
IPR007010. PolA_pol_RNA-bd_dom.
IPR014492. PolyA_polymerase.
IPR002934. Polymerase_NTP_transf_dom.
[Graphical view]
PANTHERiPTHR10682. PTHR10682. 1 hit.
PfamiPF01909. NTP_transf_2. 1 hit.
PF04928. PAP_central. 1 hit.
PF04926. PAP_RNA-bind. 2 hits.
[Graphical view]
SUPFAMiSSF55003. SSF55003. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPAPOG_MOUSE
AccessioniPrimary (citable) accession number: Q6PCL9
Secondary accession number(s): Q8BZC9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 17, 2006
Last sequence update: July 5, 2004
Last modified: November 2, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.