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Q6PCL9

- PAPOG_MOUSE

UniProt

Q6PCL9 - PAPOG_MOUSE

Protein

Poly(A) polymerase gamma

Gene

Papolg

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 88 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    Responsible for the post-transcriptional adenylation of the 3'-terminal of mRNA precursors and several small RNAs including signal recognition particle (SRP) RNA, nuclear 7SK RNA, U2 small nuclear RNA, and ribosomal 5S RNA.By similarity

    Catalytic activityi

    ATP + RNA(n) = diphosphate + RNA(n+1).

    Cofactori

    Binds 2 magnesium ions. Also active with manganese By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei108 – 1081ATPBy similarity
    Metal bindingi112 – 1121Magnesium 1; catalyticBy similarity
    Metal bindingi112 – 1121Magnesium 2; catalyticBy similarity
    Metal bindingi114 – 1141Magnesium 1; catalyticBy similarity
    Metal bindingi114 – 1141Magnesium 2; catalyticBy similarity
    Sitei152 – 1521Interaction with RNABy similarity
    Sitei157 – 1571Interaction with RNABy similarity
    Metal bindingi166 – 1661Magnesium 2; catalyticBy similarity
    Binding sitei166 – 1661ATPBy similarity
    Binding sitei227 – 2271ATPBy similarity
    Binding sitei236 – 2361ATPBy similarity
    Sitei327 – 3271Interaction with RNABy similarity
    Sitei398 – 3981Interaction with RNABy similarity
    Sitei531 – 5311Interaction with RNABy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi99 – 1013ATPBy similarity
    Nucleotide bindingi112 – 1143ATPBy similarity
    Nucleotide bindingi245 – 2462ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB
    3. polynucleotide adenylyltransferase activity Source: UniProtKB
    4. RNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. mRNA polyadenylation Source: RefGenome
    2. RNA polyadenylation Source: UniProtKB

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    mRNA processing

    Keywords - Ligandi

    ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding, RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Poly(A) polymerase gamma (EC:2.7.7.19)
    Short name:
    PAP-gamma
    Alternative name(s):
    Polynucleotide adenylyltransferase gamma
    SRP RNA 3'-adenylating enzyme
    Gene namesi
    Name:Papolg
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 11

    Organism-specific databases

    MGIiMGI:2442119. Papolg.

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 739739Poly(A) polymerase gammaPRO_0000253025Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N6-acetyllysineBy similarity
    Modified residuei23 – 231PhosphoserineBy similarity
    Modified residuei651 – 6511PhosphoserineBy similarity
    Modified residuei657 – 6571PhosphothreonineBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PaxDbiQ6PCL9.
    PRIDEiQ6PCL9.

    PTM databases

    PhosphoSiteiQ6PCL9.

    Expressioni

    Gene expression databases

    ArrayExpressiQ6PCL9.
    BgeeiQ6PCL9.
    CleanExiMM_PAPOLG.
    GenevestigatoriQ6PCL9.

    Structurei

    3D structure databases

    ProteinModelPortaliQ6PCL9.
    SMRiQ6PCL9. Positions 17-501.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the poly(A) polymerase family.Curated

    Phylogenomic databases

    eggNOGiCOG5186.
    GeneTreeiENSGT00390000017928.
    HOGENOMiHOG000204376.
    HOVERGENiHBG053502.
    InParanoidiQ6PCL9.
    KOiK14376.
    OMAiGECMPIP.
    OrthoDBiEOG7XH6PT.
    PhylomeDBiQ6PCL9.
    TreeFamiTF300842.

    Family and domain databases

    Gene3Di3.30.70.590. 1 hit.
    InterProiIPR002934. Nucleotidyltransferase.
    IPR011068. NuclTrfase_I_C.
    IPR007012. PolA_pol_cen_dom.
    IPR007010. PolA_pol_RNA-bd_dom.
    IPR014492. PolyA_polymerase.
    [Graphical view]
    PfamiPF01909. NTP_transf_2. 1 hit.
    PF04928. PAP_central. 1 hit.
    PF04926. PAP_RNA-bind. 1 hit.
    [Graphical view]
    PIRSFiPIRSF018425. PolyA_polymerase. 1 hit.
    SUPFAMiSSF55003. SSF55003. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q6PCL9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKEMSANTML DSQRQQKHYG ITSPISLACP KEIDHIYTQK LIDAMKPFGV    50
    FEDEEELNHR LVVLGKLNNL VKEWISDISE SKNLPPSVVA TVGGKIFTFG 100
    SYRLGVHTKG ADIDALCVAP RHVERSDFFQ SFFEKLKHQD GIRNLRAVED 150
    AFVPVIKFEF DGIEIDLVFA RLAIQTISDN LDLRDDSRLR SLDIRCIRSL 200
    NGCRVTDEIL HLVPNKETFR LTLRAVKLWA KRRGIYSNML GFLGGVSWAM 250
    LVARTCQLYP NAAASTLVHK FFLVFSKWEW PNPVLLKQPE ESNLNLPVWD 300
    PRVNPSDRYH LMPIITPAYP QQNSTYNVST STRTVMVEEF KQGLAVTDEI 350
    LQGKSDWSKL LEPPNFFQKY RHYIVLTASA STEENHLEWV GLVESKIRVL 400
    VGNLERNEFI TLAHVNPQSF PGNKEHHKAN NYVSMWFLGI IFRRVENAES 450
    VNIDLTYDIQ SFTDTVYRQA NNINMLKDGM KIEATHVKKK QLHHYLPAEI 500
    LQKKKKSLSD VSRSSGGLQS KRSSLDSTCL DSSRDTDSGT PFNSPVSANK 550
    PSNPDSPTGE IERSSAEPVA VVVEKLPSVP PAQGLSIPVI GAKVDPTAKA 600
    VSSPAVCTIP TVVGRNVIPR ITTPHNPVQG QPHLNGISNI TKNVTPKRSH 650
    SPPTDGTSKR LKDIEKFIRL ESAFKESRAA EDRKRKPMDS IGGESMPIPT 700
    IDTARKKRLP SKELPDSSSP VPANNIRVIK NSIRLTLNR 739
    Length:739
    Mass (Da):82,957
    Last modified:July 5, 2004 - v1
    Checksum:i603F1AB38DF985BE
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti364 – 3641P → T in BAC29194. (PubMed:16141072)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ103505 mRNA. Translation: AAZ80292.1.
    AK035810 mRNA. Translation: BAC29194.1.
    AL662810 Genomic DNA. Translation: CAI25660.1.
    BC059271 mRNA. Translation: AAH59271.1.
    CCDSiCCDS24481.1.
    RefSeqiNP_766143.2. NM_172555.2.
    UniGeneiMm.470599.

    Genome annotation databases

    EnsembliENSMUST00000020513; ENSMUSP00000020513; ENSMUSG00000020273.
    GeneIDi216578.
    KEGGimmu:216578.
    UCSCiuc007ifp.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ103505 mRNA. Translation: AAZ80292.1 .
    AK035810 mRNA. Translation: BAC29194.1 .
    AL662810 Genomic DNA. Translation: CAI25660.1 .
    BC059271 mRNA. Translation: AAH59271.1 .
    CCDSi CCDS24481.1.
    RefSeqi NP_766143.2. NM_172555.2.
    UniGenei Mm.470599.

    3D structure databases

    ProteinModelPortali Q6PCL9.
    SMRi Q6PCL9. Positions 17-501.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q6PCL9.

    Proteomic databases

    PaxDbi Q6PCL9.
    PRIDEi Q6PCL9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000020513 ; ENSMUSP00000020513 ; ENSMUSG00000020273 .
    GeneIDi 216578.
    KEGGi mmu:216578.
    UCSCi uc007ifp.2. mouse.

    Organism-specific databases

    CTDi 64895.
    MGIi MGI:2442119. Papolg.

    Phylogenomic databases

    eggNOGi COG5186.
    GeneTreei ENSGT00390000017928.
    HOGENOMi HOG000204376.
    HOVERGENi HBG053502.
    InParanoidi Q6PCL9.
    KOi K14376.
    OMAi GECMPIP.
    OrthoDBi EOG7XH6PT.
    PhylomeDBi Q6PCL9.
    TreeFami TF300842.

    Miscellaneous databases

    NextBioi 375224.
    PROi Q6PCL9.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q6PCL9.
    Bgeei Q6PCL9.
    CleanExi MM_PAPOLG.
    Genevestigatori Q6PCL9.

    Family and domain databases

    Gene3Di 3.30.70.590. 1 hit.
    InterProi IPR002934. Nucleotidyltransferase.
    IPR011068. NuclTrfase_I_C.
    IPR007012. PolA_pol_cen_dom.
    IPR007010. PolA_pol_RNA-bd_dom.
    IPR014492. PolyA_polymerase.
    [Graphical view ]
    Pfami PF01909. NTP_transf_2. 1 hit.
    PF04928. PAP_central. 1 hit.
    PF04926. PAP_RNA-bind. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF018425. PolyA_polymerase. 1 hit.
    SUPFAMi SSF55003. SSF55003. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Comparative analysis of the sequences of the SRP 3'-adenylating enzyme in human, mouse and Xenopus."
      Bhattacharya R., Gaikwad A., Reddy R.
      Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Cerebellum.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J and FVB/N.
      Tissue: Mammary tumor.
    5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    6. "The phagosomal proteome in interferon-gamma-activated macrophages."
      Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
      Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiPAPOG_MOUSE
    AccessioniPrimary (citable) accession number: Q6PCL9
    Secondary accession number(s): Q8BZC9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 17, 2006
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 88 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3