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Protein

Polypeptide N-acetylgalactosaminyltransferase 2

Gene

Galnt2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Has a broad spectrum of substrates for peptides such as EA2, Muc5AC, Muc1a, Muc1b. Probably involved in O-linked glycosylation of the immunoglobulin A1 (IgA1) hinge region (By similarity).By similarity

Catalytic activityi

UDP-N-acetyl-alpha-D-galactosamine + polypeptide = UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide.

Cofactori

Mn2+By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei142SubstrateBy similarity1
Binding sitei175SubstrateBy similarity1
Binding sitei200SubstrateBy similarity1
Metal bindingi223ManganeseBy similarity1
Binding sitei224SubstrateBy similarity1
Metal bindingi225ManganeseBy similarity1
Binding sitei330SubstrateBy similarity1
Metal bindingi358ManganeseBy similarity1
Binding sitei361SubstrateBy similarity1
Binding sitei364SubstrateBy similarity1
Binding sitei366SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Lectin, Manganese, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-6811436. COPI-independent Golgi-to-ER retrograde traffic.
R-MMU-913709. O-linked glycosylation of mucins.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiCBM13. Carbohydrate-Binding Module Family 13.
GT27. Glycosyltransferase Family 27.

Names & Taxonomyi

Protein namesi
Recommended name:
Polypeptide N-acetylgalactosaminyltransferase 2 (EC:2.4.1.41)
Alternative name(s):
Polypeptide GalNAc transferase 2
Short name:
GalNAc-T2
Short name:
pp-GaNTase 2
Protein-UDP acetylgalactosaminyltransferase 2
UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 2
Cleaved into the following chain:
Gene namesi
Name:Galnt2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:894694. Galnt2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 6CytoplasmicSequence analysis6
Transmembranei7 – 24Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST18
Topological domaini25 – 570LumenalSequence analysisAdd BLAST546

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002233921 – 570Polypeptide N-acetylgalactosaminyltransferase 2Add BLAST570
ChainiPRO_000001226751 – 570Polypeptide N-acetylgalactosaminyltransferase 2 soluble formAdd BLAST520

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi125 ↔ 353PROSITE-ProRule annotation
Disulfide bondi344 ↔ 422PROSITE-ProRule annotation
Disulfide bondi455 ↔ 472PROSITE-ProRule annotation
Disulfide bondi495 ↔ 512PROSITE-ProRule annotation
Glycosylationi515N-linked (GlcNAc...)Sequence analysis1
Modified residuei535PhosphoserineBy similarity1
Disulfide bondi538 ↔ 554PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ6PB93.
PeptideAtlasiQ6PB93.
PRIDEiQ6PB93.

PTM databases

PhosphoSitePlusiQ6PB93.
SwissPalmiQ6PB93.

Expressioni

Tissue specificityi

Widely expressed at high level.1 Publication

Gene expression databases

BgeeiENSMUSG00000089704.
GenevisibleiQ6PB93. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000034458.

Structurei

3D structure databases

ProteinModelPortaliQ6PB93.
SMRiQ6PB93.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini442 – 565Ricin B-type lectinPROSITE-ProRule annotationAdd BLAST124

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni134 – 239Catalytic subdomain AAdd BLAST106
Regioni299 – 361Catalytic subdomain BAdd BLAST63

Domaini

There are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding.By similarity
The ricin B-type lectin domain binds to GalNAc and contributes to the glycopeptide specificity.By similarity

Sequence similaritiesi

Contains 1 ricin B-type lectin domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3738. Eukaryota.
ENOG410XPRX. LUCA.
GeneTreeiENSGT00760000118828.
HOVERGENiHBG051699.
InParanoidiQ6PB93.
KOiK00710.
OMAiGKVRWPD.
OrthoDBiEOG091G085O.
PhylomeDBiQ6PB93.
TreeFamiTF313267.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR001173. Glyco_trans_2-like.
IPR029044. Nucleotide-diphossugar_trans.
IPR000772. Ricin_B_lectin.
[Graphical view]
PfamiPF00535. Glycos_transf_2. 1 hit.
PF00652. Ricin_B_lectin. 1 hit.
[Graphical view]
SMARTiSM00458. RICIN. 1 hit.
[Graphical view]
SUPFAMiSSF50370. SSF50370. 1 hit.
SSF53448. SSF53448. 1 hit.
PROSITEiPS50231. RICIN_B_LECTIN. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6PB93-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRRRSRMLLC FALLWVLGIA YYMYSGGGSA LAAGGGGAGR KGDWNDIDSI
60 70 80 90 100
KKKDLHHSRG DEKAQGVETL PPGKVRWPDF NQEAYVGGTM VRSGQDPYAR
110 120 130 140 150
NKFNQVESDK LHMDRGIPDT RHDQCQRKQW RVDLPATSVV ITFHNEARSA
160 170 180 190 200
LLRTVVSVLK RSPPHLIKEI ILVDDYSNDP EDGALLGKIE KVRVLRNDRR
210 220 230 240 250
EGLMRSRVRG ADAAQAKVLT FLDSHCECNE RWLEPLLERV AEDRTRVVSP
260 270 280 290 300
IIDVINMDNF QYVGASADLK GGFDWNLVFK WDYMTPEQRR SRQGNPVAPI
310 320 330 340 350
KTPMIAGGLF VMDKLYFEEL GKYDMMMDVW GGENLEISFR VWQCGGSLEI
360 370 380 390 400
IPCSRVGHVF RKQHPYTFPG GSGTVFARNT RRAAEVWMDE YKHFYYAAVP
410 420 430 440 450
SARNVPYGNI QSRLELRKKL GCKPFKWYLD NVYPELRVPD HQDIAFGALQ
460 470 480 490 500
QGTNCLDTLG HFADGVVGIY ECHNAGGNQE WALTKEKSVK HMDLCLTVVD
510 520 530 540 550
RSPGSLIRLQ GCRENDSRQK WEQIEGNSKL RHVGSNLCLD SRTAKSGGLS
560 570
VEVCGPALSQ QWKFSLNLQQ
Length:570
Mass (Da):64,514
Last modified:July 5, 2004 - v1
Checksum:i90D5DC02C85A8EEA
GO
Isoform 2 (identifier: Q6PB93-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-41: MRRRSRMLLCFALLWVLGIAYYMYSGGGSALAAGGGGAGRK → MALHNPQ

Note: No experimental confirmation available.
Show »
Length:536
Mass (Da):60,985
Checksum:i58DC49843190D2E4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti2R → L in AAK37548 (Ref. 1) Curated1
Sequence conflicti226C → Y in BAC32790 (PubMed:16141072).Curated1
Sequence conflicti516 – 518DSR → NSK in AAK37548 (Ref. 1) Curated3
Sequence conflicti564F → V in AAK37548 (Ref. 1) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0112011 – 41MRRRS…GAGRK → MALHNPQ in isoform 2. 1 PublicationAdd BLAST41

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF348968 mRNA. Translation: AAK37548.1.
AK046567 mRNA. Translation: BAC32790.1.
BC007172 mRNA. Translation: AAH07172.1.
BC053063 mRNA. Translation: AAH53063.1.
BC059818 mRNA. Translation: AAH59818.1.
CCDSiCCDS22769.1. [Q6PB93-1]
RefSeqiNP_644678.2. NM_139272.2. [Q6PB93-1]
UniGeneiMm.33808.

Genome annotation databases

EnsembliENSMUST00000034458; ENSMUSP00000034458; ENSMUSG00000089704. [Q6PB93-1]
ENSMUST00000127664; ENSMUSP00000118564; ENSMUSG00000092329. [Q6PB93-2]
GeneIDi108148.
KEGGimmu:108148.
UCSCiuc009nwz.2. mouse. [Q6PB93-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - GTase

Polypeptide N-acetylgalactosaminyltransferase 2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF348968 mRNA. Translation: AAK37548.1.
AK046567 mRNA. Translation: BAC32790.1.
BC007172 mRNA. Translation: AAH07172.1.
BC053063 mRNA. Translation: AAH53063.1.
BC059818 mRNA. Translation: AAH59818.1.
CCDSiCCDS22769.1. [Q6PB93-1]
RefSeqiNP_644678.2. NM_139272.2. [Q6PB93-1]
UniGeneiMm.33808.

3D structure databases

ProteinModelPortaliQ6PB93.
SMRiQ6PB93.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000034458.

Protein family/group databases

CAZyiCBM13. Carbohydrate-Binding Module Family 13.
GT27. Glycosyltransferase Family 27.

PTM databases

PhosphoSitePlusiQ6PB93.
SwissPalmiQ6PB93.

Proteomic databases

PaxDbiQ6PB93.
PeptideAtlasiQ6PB93.
PRIDEiQ6PB93.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034458; ENSMUSP00000034458; ENSMUSG00000089704. [Q6PB93-1]
ENSMUST00000127664; ENSMUSP00000118564; ENSMUSG00000092329. [Q6PB93-2]
GeneIDi108148.
KEGGimmu:108148.
UCSCiuc009nwz.2. mouse. [Q6PB93-1]

Organism-specific databases

CTDi2590.
MGIiMGI:894694. Galnt2.

Phylogenomic databases

eggNOGiKOG3738. Eukaryota.
ENOG410XPRX. LUCA.
GeneTreeiENSGT00760000118828.
HOVERGENiHBG051699.
InParanoidiQ6PB93.
KOiK00710.
OMAiGKVRWPD.
OrthoDBiEOG091G085O.
PhylomeDBiQ6PB93.
TreeFamiTF313267.

Enzyme and pathway databases

UniPathwayiUPA00378.
ReactomeiR-MMU-6811436. COPI-independent Golgi-to-ER retrograde traffic.
R-MMU-913709. O-linked glycosylation of mucins.

Miscellaneous databases

PROiQ6PB93.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000089704.
GenevisibleiQ6PB93. MM.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR001173. Glyco_trans_2-like.
IPR029044. Nucleotide-diphossugar_trans.
IPR000772. Ricin_B_lectin.
[Graphical view]
PfamiPF00535. Glycos_transf_2. 1 hit.
PF00652. Ricin_B_lectin. 1 hit.
[Graphical view]
SMARTiSM00458. RICIN. 1 hit.
[Graphical view]
SUPFAMiSSF50370. SSF50370. 1 hit.
SSF53448. SSF53448. 1 hit.
PROSITEiPS50231. RICIN_B_LECTIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGALT2_MOUSE
AccessioniPrimary (citable) accession number: Q6PB93
Secondary accession number(s): Q7TSI5
, Q8BL27, Q922K5, Q99ME1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: July 5, 2004
Last modified: November 2, 2016
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.