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Protein

Non-canonical poly(A) RNA polymerase PAPD7

Gene

Papd7

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalytic subunit of a TRAMP-like complex which has a poly(A) RNA polymerase activity and is involved in a post-transcriptional quality control mechanism. Polyadenylation with short oligo(A) tails is required for the degradative activity of the exosome on several of its nuclear RNA substrates. Has no terminal uridylyltransferase activity, and does not play a role in replication-dependent histone mRNA degradation via uridylation (By similarity).By similarity

Catalytic activityi

ATP + RNA(n) = diphosphate + RNA(n+1).

Cofactori

Mg2+By similarity, Mn2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi47Magnesium or manganese; catalyticBy similarity1
Metal bindingi49Magnesium or manganese; catalyticBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, Transferase
Biological processmRNA processing
LigandATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Non-canonical poly(A) RNA polymerase PAPD7 (EC:2.7.7.19)
Alternative name(s):
DNA polymerase sigma
PAP-associated domain-containing protein 7
TRAMP-like complex polyadenylate polymerase
Gene namesi
Name:Papd7
Synonyms:Pols
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:2682295. Papd7.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001203091 – 542Non-canonical poly(A) RNA polymerase PAPD7Add BLAST542

Proteomic databases

PaxDbiQ6PB75.
PRIDEiQ6PB75.

PTM databases

iPTMnetiQ6PB75.
PhosphoSitePlusiQ6PB75.

Expressioni

Gene expression databases

BgeeiENSMUSG00000034575.
CleanExiMM_POLS.
ExpressionAtlasiQ6PB75. baseline and differential.
GenevisibleiQ6PB75. MM.

Interactioni

Subunit structurei

Component of a nuclear TRAMP-like complex, an ATP-dependent exosome regulatory complex consisting of a helicase (MTREX), an oligadenylate polymerase (PAPD5 or PAPD7), and a substrate specific RNA-binding factor (ZCCHC7 or ZCCHC8). Several TRAMP-like complexes exist with specific compositions and are associated with nuclear, or nucleolar RNA exosomes.

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000040757.

Structurei

3D structure databases

ProteinModelPortaliQ6PB75.
SMRiQ6PB75.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini178 – 238PAP-associatedAdd BLAST61

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi332 – 372Ser-richAdd BLAST41

Sequence similaritiesi

Belongs to the DNA polymerase type-B-like family.Curated

Phylogenomic databases

eggNOGiKOG1906. Eukaryota.
COG5260. LUCA.
GeneTreeiENSGT00400000022055.
HOGENOMiHOG000290646.
HOVERGENiHBG053636.
InParanoidiQ6PB75.
KOiK03514.
TreeFamiTF313939.

Family and domain databases

InterProiView protein in InterPro
IPR002058. PAP_assoc.
IPR002934. Polymerase_NTP_transf_dom.
PfamiView protein in Pfam
PF01909. NTP_transf_2. 1 hit.
PF03828. PAP_assoc. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6PB75-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSPCPEEAAM RREVVKRIET VVKDLWPTAD VQIFGSFSTG LYLPTSDIDL
60 70 80 90 100
VVFGKWERPP LQLLEQALRK HNVAEPCSIK VLDKATVPII KLTDQETEVK
110 120 130 140 150
VDISFNMETG VRAAEFIKNY MKKYSLLPYL ILVLKQFLLQ RDLNEVFTGG
160 170 180 190 200
ISSYSLILMA ISFLQLHPRI DARRADENLG MLLVEFFELY GRNFNYLKTG
210 220 230 240 250
IRIKEGGAYI AKEEIMKAMT SGYRPSMLCI EDPLLPGNDV GRSSYGAMQV
260 270 280 290 300
KQVFDYAYIV LSHAVSPLAR SYPNRDSEST LGRIIKVTQE VIDYRRWIKE
310 320 330 340 350
KWGSRILPSP DLDNRIKIKE RITTCNGEQM QSREPSSPYT QRLTLSLSSP
360 370 380 390 400
QLLSSGSSAS SVSSLSGSDI DSDTPPCTTP SVYQFSLQAP TTLMASLPTA
410 420 430 440 450
LPMPSSKPQP AASRTLIMTT NNQTRVTIPP PTLGVAPVPC RQAGVDGTTS
460 470 480 490 500
LKAVHSVTSP AIPSASPNPL SSPHLYHKQH NGMKLSMKGS HNHTQGGGYS
510 520 530 540
SVGSGAVRPP VGNRGHHQYN RTGWRRKKHA HTRDSLPVSL SR
Length:542
Mass (Da):60,027
Last modified:September 18, 2013 - v2
Checksum:i9B9DC7D11F886045
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti297W → R in AAH59846 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC122484 Genomic DNA. No translation available.
CH466563 Genomic DNA. Translation: EDL37014.1.
BC059846 mRNA. Translation: AAH59846.1.
CCDSiCCDS26622.1.
RefSeqiNP_941002.2. NM_198600.2.
UniGeneiMm.315959.

Genome annotation databases

EnsembliENSMUST00000044081; ENSMUSP00000040757; ENSMUSG00000034575.
ENSMUST00000223344; ENSMUSP00000152244; ENSMUSG00000034575.
GeneIDi210106.
KEGGimmu:210106.
UCSCiuc007rch.2. mouse.

Similar proteinsi

Entry informationi

Entry nameiPAPD7_MOUSE
AccessioniPrimary (citable) accession number: Q6PB75
Secondary accession number(s): G3X948
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: September 18, 2013
Last modified: January 31, 2018
This is version 99 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Was originally thought to have DNA polymerase activity.Curated

Keywords - Technical termi

Complete proteome, Reference proteome