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Protein

Leucine-rich PPR motif-containing protein, mitochondrial

Gene

Lrpprc

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in RNA metabolism in both nuclei and mitochondria. In the nucleus binds to HNRPA1-associated poly(A) mRNAs and is part of nmRNP complexes at late stages of mRNA maturation which are possibly associated with nuclear mRNA export. May bind mature mRNA in the nucleus outer membrane. In mitochondria binds to poly(A) mRNA. Plays a role in translation or stability of mitochondrially encoded cytochrome c oxidase (COX) subunits. May be involved in transcription regulation. Cooperates with PPARGC1A to regulate certain mitochondrially encoded genes and gluconeogenic genes and may regulate docking of PPARGC1A to transcription factors. Seems to be involved in the transcription regulation of the multidrug-related genes MDR1 and MVP. Part of a nuclear factor that binds to the invMED1 element of MDR1 and MVP gene promoters (By similarity). Binds single-stranded DNA.By similarity

GO - Molecular functioni

  • beta-tubulin binding Source: HGNC
  • poly(A) RNA binding Source: MGI
  • RNA binding Source: MGI
  • single-stranded DNA binding Source: MGI
  • ubiquitin protein ligase binding Source: MGI

GO - Biological processi

  • mRNA transport Source: UniProtKB-KW
  • negative regulation of mitochondrial RNA catabolic process Source: MGI
  • regulation of mitochondrial translation Source: MGI
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

mRNA transport, Transcription, Transcription regulation, Transport

Keywords - Ligandi

DNA-binding, RNA-binding

Enzyme and pathway databases

ReactomeiR-MMU-5628897. TP53 Regulates Metabolic Genes.
R-MMU-611105. Respiratory electron transport.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine-rich PPR motif-containing protein, mitochondrial
Alternative name(s):
130 kDa leucine-rich protein
Short name:
LRP 130
Short name:
mLRP130
Gene namesi
Name:Lrpprc
Synonyms:Lrp130
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:1919666. Lrpprc.

Subcellular locationi

GO - Cellular componenti

  • condensed nuclear chromosome Source: HGNC
  • cytoplasm Source: MGI
  • cytoskeleton Source: HGNC
  • intracellular ribonucleoprotein complex Source: MGI
  • membrane Source: MGI
  • microtubule Source: MGI
  • mitochondrial nucleoid Source: MGI
  • mitochondrion Source: UniProtKB
  • nuclear inner membrane Source: UniProtKB-SubCell
  • nuclear outer membrane Source: UniProtKB-SubCell
  • nucleoplasm Source: UniProtKB-SubCell
  • nucleus Source: HGNC
  • perinuclear region of cytoplasm Source: HGNC
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 5959MitochondrionSequence analysisAdd
BLAST
Chaini60 – 13921333Leucine-rich PPR motif-containing protein, mitochondrialPRO_0000295546Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei151 – 1511N6-acetyllysineCombined sources
Modified residuei186 – 1861N6-acetyllysineCombined sources
Modified residuei225 – 2251N6-acetyllysineCombined sources
Modified residuei291 – 2911N6-acetyllysineBy similarity
Modified residuei462 – 4621N6-acetyllysineCombined sources
Modified residuei749 – 7491N6-acetyllysineBy similarity
Modified residuei1026 – 10261PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ6PB66.
MaxQBiQ6PB66.
PaxDbiQ6PB66.
PRIDEiQ6PB66.

PTM databases

iPTMnetiQ6PB66.
PhosphoSiteiQ6PB66.
SwissPalmiQ6PB66.

Expressioni

Tissue specificityi

Strongly expressed in heart, liver and kidney. Weakly expressed in brain, skeletal muscle and testes.1 Publication

Developmental stagei

Expressed at embryonic stages E7, E11, E15 and E17 with a slight increase of levels during development.1 Publication

Gene expression databases

BgeeiQ6PB66.
CleanExiMM_LRPPRC.
ExpressionAtlasiQ6PB66. baseline and differential.
GenevisibleiQ6PB66. MM.

Interactioni

Subunit structurei

Interacts with CECR2, HEBP2, MAP1S and UXT (By similarity). Interacts with PPARGC1A (By similarity). Interacts with FOXO1. Component of mRNP complexes associated with HNRPA1 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Foxo1Q9R1E02EBI-1371262,EBI-1371343
Ppargc1aO703432EBI-1371262,EBI-1371053

GO - Molecular functioni

Protein-protein interaction databases

BioGridi215365. 2 interactions.
IntActiQ6PB66. 6 interactions.
MINTiMINT-1840300.
STRINGi10090.ENSMUSP00000107927.

Structurei

3D structure databases

ProteinModelPortaliQ6PB66.
SMRiQ6PB66. Positions 117-325, 956-981, 1105-1130, 1178-1203.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati125 – 15935PPR 1Add
BLAST
Repeati160 – 19435PPR 2Add
BLAST
Repeati195 – 22935PPR 3Add
BLAST
Repeati230 – 26435PPR 4Add
BLAST
Repeati265 – 29935PPR 5Add
BLAST
Repeati300 – 33435PPR 6Add
BLAST
Repeati402 – 43635PPR 7Add
BLAST
Repeati437 – 47135PPR 8Add
BLAST
Repeati677 – 70832PPR 9Add
BLAST
Repeati709 – 74537PPR 10Add
BLAST
Repeati746 – 78338PPR 11Add
BLAST
Repeati784 – 82037PPR 12Add
BLAST
Repeati821 – 85636PPR 13Add
BLAST
Repeati953 – 98735PPR 14Add
BLAST
Repeati1030 – 106435PPR 15Add
BLAST
Repeati1065 – 110137PPR 16Add
BLAST
Repeati1102 – 113635PPR 17Add
BLAST
Repeati1137 – 117539PPR 18Add
BLAST
Repeati1176 – 121035PPR 19Add
BLAST
Repeati1315 – 134935PPR 20Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni931 – 1050120RNA-bindingAdd
BLAST

Sequence similaritiesi

Contains 20 PPR (pentatricopeptide) repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transit peptide

Phylogenomic databases

eggNOGiKOG4318. Eukaryota.
ENOG410XSG9. LUCA.
GeneTreeiENSGT00390000016775.
HOGENOMiHOG000113350.
HOVERGENiHBG097314.
InParanoidiQ6PB66.
KOiK17964.
OMAiPIRQHYF.
OrthoDBiEOG72JWG1.
PhylomeDBiQ6PB66.
TreeFamiTF323626.

Family and domain databases

InterProiIPR002885. Pentatricopeptide_repeat.
[Graphical view]
PfamiPF01535. PPR. 1 hit.
PF13812. PPR_3. 2 hits.
[Graphical view]
TIGRFAMsiTIGR00756. PPR. 2 hits.
PROSITEiPS51375. PPR. 13 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6PB66-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAALLRPARW LLGAAAAPRL PLSLRLPAGV PGRLSSVVRV AAVGSRPAAG
60 70 80 90 100
ERLSQARLYA IVAEKRDLQE EPAPVRKNSS QFDWALMRLD NSVRRTGRIT
110 120 130 140 150
KGLLQRVFES TCSSGSPGSN QALLLLRSCG SLLPELSLAE RTEFAHKIWD
160 170 180 190 200
KLQQLGVVYD VSHYNALLKV YLQNEYKFSP TDFLAKMEGA NIQPNRVTYQ
210 220 230 240 250
RLIAAYCNVG DIEGASKILG FMKTKDLPIT EAVFSALVTG HARAGDMENA
260 270 280 290 300
ENILTVMKQA GIEPGPDTYL ALLNAHAERG DIGQVRQILE KVEKSDHYFM
310 320 330 340 350
DRDFLQVIFS FSKAGYPQYV SEILEKITYE RRSIPDAMNL ILFLATEKLE
360 370 380 390 400
DTAFQVLLAL PLSKDESSDN FGSFFLRHCV TLDLPPEKLI DYCRRLRDAK
410 420 430 440 450
LHSSSLQFTL HCALQANRTA LAKAVMEALR EEGFPIRPHY FWPLLAGHQK
460 470 480 490 500
TKNVQGIIDI LKIMNKVGVD PDQETYINYV FPCFDSAQSV RAALQENECL
510 520 530 540 550
LASSTFAQAE VKNEAINGNL QNILSFLESN TLPFSFSSLR NSLILGFRRS
560 570 580 590 600
MNIDLWSKIT ELLYKDERYC SKPPGPAEAV GYFLYNLIDS MSDSEVQAKE
610 620 630 640 650
ERLRQYFHQL QEMNVKVPEN IYKGICNLLN TYHVPELIKD IKVLVDREKV
660 670 680 690 700
DSQKTSQVTS SDLESTLEKL KAEGQPVGSA LKQLLLLLCS EENMQKALEV
710 720 730 740 750
KAKYESDMVI GGYAALINLC CRHDNAEDAW NLKQEVDRLD ASAILDTAKY
760 770 780 790 800
VALVKVLGKH SRLQDAINIL KEMKEKDVVI KDATVLSFFH ILNGAALRGE
810 820 830 840 850
IETVKQLHEA IVTLGLAKPS SNISFPLVTV HLEKGDLPAA LEASIACHKK
860 870 880 890 900
YKVLPRIHDV LCKLVEKGET DLIQKAMDFV SQEQGEMTML YDLFFAFLQT
910 920 930 940 950
GNYKEAKKII ETPGIRARPT RLQWFCDRCI ASNQVEALEK LVELTEKLFE
960 970 980 990 1000
CDRDQMYYNL LKLYKISSDW QRADAAWTKM QEENIIPRER TLRLLAEILK
1010 1020 1030 1040 1050
TSNQEVPFDV PELWFGDDRP SLSPSSRSAG EDVTEKTLLS NCKLKKSKDA
1060 1070 1080 1090 1100
YNIFLKAEKQ NVVFSSETYS TLIGLLLSKD DFTQAMHVKD FAETHIKGFT
1110 1120 1130 1140 1150
LNDAANSLLI IRQVRRDYLK GALATLRAAL DLKQVPSQIA VTRLIQALAL
1160 1170 1180 1190 1200
KGDVESIEAI QRMVAGLDTI GLSKMVFINN IALAQMKNNK LDAAIENIEH
1210 1220 1230 1240 1250
LLASENQAIE PQYFGLSYLF RKVIEEQMEP ALEKLSIMSE RMANQFALYK
1260 1270 1280 1290 1300
PVTDLFLQLV DSGKVDEARA LLERCGAIAE QSSLLSVFCL RTSQKPKKAP
1310 1320 1330 1340 1350
VLKTLLELIP ELRDNDKVYS CSMKSYALDK DVASAKALYE YLTAKNLKLD
1360 1370 1380 1390
DLFLKRYAAL LKDVGEPVPF PEPPESFAFY IKQLKEARES PS
Length:1,392
Mass (Da):156,615
Last modified:July 24, 2007 - v2
Checksum:iCC774240FCDBB2A6
GO

Sequence cautioni

The sequence AAH59862.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence BAB29082.2 differs from that shown. Reason: Erroneous translation. CTG leucine codon is translated as initiator methionine.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti35 – 351S → F in BAB93528 (PubMed:12071956).Curated
Sequence conflicti52 – 521R → L in BAB93528 (PubMed:12071956).Curated
Sequence conflicti67 – 671D → V in BAB93528 (PubMed:12071956).Curated
Sequence conflicti466 – 4661K → E in BAB93528 (PubMed:12071956).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB027124 mRNA. Translation: BAB93528.1. Sequence problems.
BC059862 mRNA. Translation: AAH59862.1. Different initiation.
AK013955 mRNA. Translation: BAB29082.2. Sequence problems.
CCDSiCCDS29003.2.
RefSeqiNP_082509.2. NM_028233.2.
UniGeneiMm.217027.

Genome annotation databases

EnsembliENSMUST00000112308; ENSMUSP00000107927; ENSMUSG00000024120.
GeneIDi72416.
KEGGimmu:72416.
UCSCiuc008dtc.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB027124 mRNA. Translation: BAB93528.1. Sequence problems.
BC059862 mRNA. Translation: AAH59862.1. Different initiation.
AK013955 mRNA. Translation: BAB29082.2. Sequence problems.
CCDSiCCDS29003.2.
RefSeqiNP_082509.2. NM_028233.2.
UniGeneiMm.217027.

3D structure databases

ProteinModelPortaliQ6PB66.
SMRiQ6PB66. Positions 117-325, 956-981, 1105-1130, 1178-1203.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi215365. 2 interactions.
IntActiQ6PB66. 6 interactions.
MINTiMINT-1840300.
STRINGi10090.ENSMUSP00000107927.

PTM databases

iPTMnetiQ6PB66.
PhosphoSiteiQ6PB66.
SwissPalmiQ6PB66.

Proteomic databases

EPDiQ6PB66.
MaxQBiQ6PB66.
PaxDbiQ6PB66.
PRIDEiQ6PB66.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000112308; ENSMUSP00000107927; ENSMUSG00000024120.
GeneIDi72416.
KEGGimmu:72416.
UCSCiuc008dtc.1. mouse.

Organism-specific databases

CTDi10128.
MGIiMGI:1919666. Lrpprc.

Phylogenomic databases

eggNOGiKOG4318. Eukaryota.
ENOG410XSG9. LUCA.
GeneTreeiENSGT00390000016775.
HOGENOMiHOG000113350.
HOVERGENiHBG097314.
InParanoidiQ6PB66.
KOiK17964.
OMAiPIRQHYF.
OrthoDBiEOG72JWG1.
PhylomeDBiQ6PB66.
TreeFamiTF323626.

Enzyme and pathway databases

ReactomeiR-MMU-5628897. TP53 Regulates Metabolic Genes.
R-MMU-611105. Respiratory electron transport.

Miscellaneous databases

ChiTaRSiLrpprc. mouse.
NextBioi336222.
PROiQ6PB66.
SOURCEiSearch...

Gene expression databases

BgeeiQ6PB66.
CleanExiMM_LRPPRC.
ExpressionAtlasiQ6PB66. baseline and differential.
GenevisibleiQ6PB66. MM.

Family and domain databases

InterProiIPR002885. Pentatricopeptide_repeat.
[Graphical view]
PfamiPF01535. PPR. 1 hit.
PF13812. PPR_3. 2 hits.
[Graphical view]
TIGRFAMsiTIGR00756. PPR. 2 hits.
PROSITEiPS51375. PPR. 13 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "LRP130, a protein containing nine pentatricopeptide repeat motifs, interacts with a single-stranded cytosine-rich sequence of mouse hypervariable minisatellite Pc-1."
    Tsuchiya N., Fukuda H., Sugimura T., Nagao M., Nakagama H.
    Eur. J. Biochem. 269:2927-2933(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], IDENTIFICATION BY MASS SPECTROMETRY, DNA-BINDING, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Brain.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 681-1392.
    Strain: C57BL/6J.
    Tissue: Head.
  4. "LRP130, a single-stranded DNA/RNA-binding protein, localizes at the outer nuclear and endoplasmic reticulum membrane, and interacts with mRNA in vivo."
    Tsuchiya N., Fukuda H., Nakashima K., Nagao M., Sugimura T., Nakagama H.
    Biochem. Biophys. Res. Commun. 317:736-743(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: RNA-BINDING.
  5. "Defects in energy homeostasis in Leigh syndrome French Canadian variant through PGC-1alpha/LRP130 complex."
    Cooper M.P., Qu L., Rohas L.M., Lin J., Yang W., Erdjument-Bromage H., Tempst P., Spiegelman B.M.
    Genes Dev. 20:2996-3009(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH FOXO1.
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.
  7. "Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways."
    Rardin M.J., Newman J.C., Held J.M., Cusack M.P., Sorensen D.J., Li B., Schilling B., Mooney S.D., Kahn C.R., Verdin E., Gibson B.W.
    Proc. Natl. Acad. Sci. U.S.A. 110:6601-6606(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-151; LYS-186; LYS-225 AND LYS-462, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiLPPRC_MOUSE
AccessioniPrimary (citable) accession number: Q6PB66
Secondary accession number(s): Q8K4V0, Q9CRX4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 24, 2007
Last sequence update: July 24, 2007
Last modified: May 11, 2016
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.