Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Diacylglycerol O-acyltransferase 2

Gene

dgat2

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Essential acyltransferase that catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for synthesis and storage of intracellular triglycerides. Probably plays a central role in cytosolic lipid accumulation (By similarity).By similarity

Catalytic activityi

Acyl-CoA + 1,2-diacylglycerol = CoA + triacylglycerol.

Pathwayi

GO - Molecular functioni

  1. diacylglycerol O-acyltransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. glycerol metabolic process Source: UniProtKB-KW
  2. triglyceride biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Glycerol metabolism, Lipid biosynthesis, Lipid metabolism

Enzyme and pathway databases

UniPathwayiUPA00282.

Names & Taxonomyi

Protein namesi
Recommended name:
Diacylglycerol O-acyltransferase 2 (EC:2.3.1.20)
Alternative name(s):
Diglyceride acyltransferase 2
Gene namesi
Name:dgat2
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-6078761. dgat2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 4242CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei43 – 6119HelicalSequence AnalysisAdd
BLAST
Topological domaini62 – 654LumenalSequence Analysis
Transmembranei66 – 8520HelicalSequence AnalysisAdd
BLAST
Topological domaini86 – 361276CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 361361Diacylglycerol O-acyltransferase 2PRO_0000249049Add
BLAST

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENiHBG065791.
KOiK11160.

Family and domain databases

InterProiIPR007130. DAGAT.
IPR002123. Plipid/glycerol_acylTrfase.
[Graphical view]
PANTHERiPTHR12317. PTHR12317. 1 hit.
PfamiPF03982. DAGAT. 1 hit.
[Graphical view]
SMARTiSM00563. PlsC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6PAZ3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTIIAAYSG VLRGTGSSLL SAVHDLPSIP WLSKSSVVRH LQIISVLQWV
60 70 80 90 100
LSFLILGVAC TAVLVYIFCT DLWLIAALYF TWMVLDWNTP YKGGRRSSWV
110 120 130 140 150
RNWAVWRYFR DYFPVKLVKT HNLLPSRNYI FGYHPHGIMC LGAFCNFGTE
160 170 180 190 200
ATGVSKKFPG IKCHLATLAG NFRMPVLREY LMSGGICPVN RDTINYILSK
210 220 230 240 250
NGTGNAVVIA VGGAAESLNC RPGKNTVTLL HRKGFVKVAL QHGADLVPIY
260 270 280 290 300
SFGENETYKQ VVFEEGSWGR WIQQKFQKYV GFAPCLFHGC SFFSSNSWGL
310 320 330 340 350
VPYANPITTV VGEPITVPKI EQPTQKDVEL YHSMYLSSLH RLFDKYKTKL
360
GLPDSETLEF I
Length:361
Mass (Da):40,568
Last modified:July 5, 2004 - v1
Checksum:i05DAFBB1037282A1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC059991 mRNA. Translation: AAH59991.1.
RefSeqiNP_001083204.1. NM_001089735.1.
UniGeneiXl.25952.

Genome annotation databases

GeneIDi398800.
KEGGixla:398800.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC059991 mRNA. Translation: AAH59991.1.
RefSeqiNP_001083204.1. NM_001089735.1.
UniGeneiXl.25952.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi398800.
KEGGixla:398800.

Organism-specific databases

CTDi84649.
XenbaseiXB-GENE-6078761. dgat2.

Phylogenomic databases

HOVERGENiHBG065791.
KOiK11160.

Enzyme and pathway databases

UniPathwayiUPA00282.

Family and domain databases

InterProiIPR007130. DAGAT.
IPR002123. Plipid/glycerol_acylTrfase.
[Graphical view]
PANTHERiPTHR12317. PTHR12317. 1 hit.
PfamiPF03982. DAGAT. 1 hit.
[Graphical view]
SMARTiSM00563. PlsC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. NIH - Xenopus Gene Collection (XGC) project
    Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.

Entry informationi

Entry nameiDGAT2_XENLA
AccessioniPrimary (citable) accession number: Q6PAZ3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: July 5, 2004
Last modified: January 7, 2015
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.