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Q6PAW2

- UBP16_XENLA

UniProt

Q6PAW2 - UBP16_XENLA

Protein

Ubiquitin carboxyl-terminal hydrolase 16

Gene

usp16

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 64 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    Specifically deubiquitinates 'Lys-120' of histone H2A (H2AK119Ub), a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A is a prerequisite for subsequent phosphorylation at 'Ser-11' of histone H3 (H3S10ph), and is required for chromosome segregation when cells enter into mitosis. Regulates Hox gene expression via histone H2A deubiquitination. Prefers nucleosomal substrates. Does not deubiquitinate histone H2B.UniRule annotation

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi21 – 211Zinc 1UniRule annotation
    Metal bindingi23 – 231Zinc 1UniRule annotation
    Metal bindingi45 – 451Zinc 2UniRule annotation
    Metal bindingi48 – 481Zinc 2UniRule annotation
    Metal bindingi71 – 711Zinc 3UniRule annotation
    Metal bindingi74 – 741Zinc 3UniRule annotation
    Metal bindingi79 – 791Zinc 2UniRule annotation
    Metal bindingi87 – 871Zinc 2UniRule annotation
    Metal bindingi91 – 911Zinc 3UniRule annotation
    Metal bindingi100 – 1001Zinc 3UniRule annotation
    Metal bindingi113 – 1131Zinc 1UniRule annotation
    Metal bindingi116 – 1161Zinc 1UniRule annotation
    Active sitei206 – 2061NucleophileUniRule annotation
    Active sitei835 – 8351Proton acceptorUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri43 – 12280UBP-typeUniRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. cysteine-type endopeptidase activity Source: UniProtKB
    2. histone binding Source: UniProtKB
    3. transcription coactivator activity Source: UniProtKB
    4. ubiquitin binding Source: UniProtKB
    5. ubiquitin-specific protease activity Source: UniProtKB
    6. ubiquitin thiolesterase activity Source: UniProtKB
    7. zinc ion binding Source: UniProtKB

    GO - Biological processi

    1. anterior/posterior pattern specification Source: UniProtKB
    2. histone deubiquitination Source: UniProtKB
    3. mitotic nuclear division Source: UniProtKB
    4. positive regulation of transcription, DNA-templated Source: UniProtKB
    5. protein homotetramerization Source: UniProtKB
    6. transcription, DNA-templated Source: UniProtKB-KW
    7. ubiquitin-dependent protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Activator, Chromatin regulator, Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis, Transcription, Transcription regulation, Ubl conjugation pathway

    Keywords - Ligandi

    Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin carboxyl-terminal hydrolase 16UniRule annotation (EC:3.4.19.12UniRule annotation)
    Alternative name(s):
    Deubiquitinating enzyme 16UniRule annotation
    Ubiquitin thioesterase 16UniRule annotation
    Ubiquitin-specific-processing protease 16UniRule annotation
    Gene namesi
    Name:usp16
    OrganismiXenopus laevis (African clawed frog)
    Taxonomic identifieri8355 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

    Organism-specific databases

    XenbaseiXB-GENE-1007170. usp16.

    Subcellular locationi

    Nucleus UniRule annotation

    GO - Cellular componenti

    1. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 901901Ubiquitin carboxyl-terminal hydrolase 16PRO_0000367506Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotation

    Structurei

    3D structure databases

    ProteinModelPortaliQ6PAW2.
    SMRiQ6PAW2. Positions 19-139.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini197 – 900704USPAdd
    BLAST

    Domaini

    The UBP-type zinc finger binds 3 zinc ions that form a pair of cross-braced ring fingers encapsulated within a third zinc finger in the primary structure. It recognizes the C-terminal tail of free ubiquitin.UniRule annotation

    Sequence similaritiesi

    Belongs to the peptidase C19 family. USP16 subfamily.UniRule annotation
    Contains 1 UBP-type zinc finger.UniRule annotation
    Contains 1 USP domain.Curated

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri43 – 12280UBP-typeUniRule annotationAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    HOVERGENiHBG062704.
    KOiK11844.

    Family and domain databases

    Gene3Di3.30.40.10. 1 hit.
    HAMAPiMF_03062. UBP16.
    InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    IPR013083. Znf_RING/FYVE/PHD.
    IPR001607. Znf_UBP.
    [Graphical view]
    PfamiPF00443. UCH. 1 hit.
    PF02148. zf-UBP. 1 hit.
    [Graphical view]
    PROSITEiPS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    PS50271. ZF_UBP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q6PAW2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVKKRGTNLP AQDFLDAEPV CKHLRKALDD GSVKRALVNV EWTMCQDCQA    50
    DNKEKNNSED ESAEDPSVWL CLKCGHRGCG RNSVAQHALN HYNTPRSEPH 100
    CLVLNVDMWS AWCYLCDNEV PYNRTSRLGQ LVDYLQRKAK AKNKNSNSVV 150
    SNEEVKTEIV TENEVKKIQY QDEKPDVQAK QEKASSTQKI STEPTVKGLS 200
    NLGNTCFFNA VMQNLSQTPA LSELLNEVKT FRKPVTVLLP DSSSPKILEV 250
    NLEQQPGPLT LAMWQFLTEM HETKKGVVTP KELFSQVCKK AIRFKGYQQQ 300
    DSQELLRYLL DGMRGEEIQR VTLAMSKSLQ STLDEEEIKK IVKDYEKRRT 350
    IPNFVDCLFG GELTSTIMCE ECHTVSLVHE PFLDLSLPVL DDLIVKKNSK 400
    STPPARERKE EEEEEENDDD RYVKERDEVS PGASKHLQKK AKKAAKKQAK 450
    NQRRQQKWQG KTVLFTDLAK QECSEDEEEV SQTKTNTRPD NETPTADGLN 500
    TMETDLSTLE NGNEESADGL NTMETDLSTL ENGNEEMAGG FKTMEMDLST 550
    LENGSETIKS AVEGITEHTD LDSSVHNNVG SVETNALVGN MENNNNIEVN 600
    KTPERTAGSG GDSMEAMAAV ENGNADAVNV DDTEAVNGLI DSANMDHELT 650
    NSLNRLQLSS DLEPTQVEIE ILPDKEQPHT QVYEVVNEDP KTAFSTLSNR 700
    KDLPIDEFSV LSCLYQFTHK ETLTGNNKLL CNVCTRKQAS RLNNSNKGEK 750
    KFVYTNAKKQ MLVSNPSPIL TLHLKRFQQN GFNLRKINRH IKFPEVLDLA 800
    PFCTAKCKNV PEGESRLLYS LYGVIEHSGS MRSGHYTAFV KLRHPNQQLC 850
    KMLFTGVIPE VSGSEPGQGS WYHISDSHVQ AVSLSRVLSS QAYLLFYERM 900
    L 901
    Length:901
    Mass (Da):101,293
    Last modified:July 5, 2004 - v1
    Checksum:iEAC737894C997ABA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC060022 mRNA. Translation: AAH60022.1.
    RefSeqiNP_001083244.1. NM_001089775.1.
    UniGeneiXl.32808.

    Genome annotation databases

    GeneIDi398823.
    KEGGixla:398823.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC060022 mRNA. Translation: AAH60022.1 .
    RefSeqi NP_001083244.1. NM_001089775.1.
    UniGenei Xl.32808.

    3D structure databases

    ProteinModelPortali Q6PAW2.
    SMRi Q6PAW2. Positions 19-139.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 398823.
    KEGGi xla:398823.

    Organism-specific databases

    CTDi 10600.
    Xenbasei XB-GENE-1007170. usp16.

    Phylogenomic databases

    HOVERGENi HBG062704.
    KOi K11844.

    Family and domain databases

    Gene3Di 3.30.40.10. 1 hit.
    HAMAPi MF_03062. UBP16.
    InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    IPR013083. Znf_RING/FYVE/PHD.
    IPR001607. Znf_UBP.
    [Graphical view ]
    Pfami PF00443. UCH. 1 hit.
    PF02148. zf-UBP. 1 hit.
    [Graphical view ]
    PROSITEi PS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    PS50271. ZF_UBP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. NIH - Xenopus Gene Collection (XGC) project
      Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Kidney.
    2. "Regulation of cell cycle progression and gene expression by H2A deubiquitination."
      Joo H.-Y., Zhai L., Yang C., Nie S., Erdjument-Bromage H., Tempst P., Chang C., Wang H.
      Nature 449:1068-1072(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiUBP16_XENLA
    AccessioniPrimary (citable) accession number: Q6PAW2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 24, 2009
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 64 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3