Q6PAW2 (UBP16_XENLA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin carboxyl-terminal hydrolase 16
EC=3.4.19.12

Alternative name(s):
Deubiquitinating enzyme 16
Ubiquitin thioesterase 16
Ubiquitin-specific-processing protease 16

Gene names

Name:

usp16

Organism

Xenopus laevis (African clawed frog)

Taxonomic identifier

8355 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amphibia › Batrachia › Anura › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length	901 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Specifically deubiquitinates histone H2A, a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A is a prerequisite for subsequent phosphorylation at 'Ser-10' of histone H3, and is required for chromosome segregation when cells enter into mitosis. Regulates Hox gene expression via histone H2A deubiquitination. Prefers nucleosomal substrates. Does not deubiquitinate histone H2B By similarity. Ref.2
Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Subunit structure	Homotetramer By similarity.
Subcellular location	Nucleus By similarity.
Domain	The UBP-type zinc finger binds 3 zinc ions that form a pair of cross-braced ring fingers encapsulated within a third zinc finger in the primary structure. It recognizes the C-terminal tail of free ubiquitin By similarity.
Sequence similarities	Belongs to the peptidase C19 family. USP16 subfamily. Contains 1 UBP-type zinc finger.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis Transcription Transcription regulation Ubl conjugation pathway
Cellular component	Nucleus
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Activator Chromatin regulator Hydrolase Protease Thiol protease
Gene Ontology (GO)
Biological_process	anterior/posterior pattern specification Inferred from mutant phenotype Ref.2. Source: UniProtKB cell division Inferred from electronic annotation. Source: UniProtKB-KW histone deubiquitination Inferred from sequence or structural similarity. Source: UniProtKB mitosis Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of transcription, DNA-dependent Inferred from sequence or structural similarity. Source: UniProtKB protein homotetramerization Inferred from sequence or structural similarity. Source: UniProtKB transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	nucleus Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	cysteine-type endopeptidase activity Inferred from sequence or structural similarity. Source: UniProtKB histone binding Inferred from sequence or structural similarity. Source: UniProtKB transcription coactivator activity Inferred from sequence or structural similarity. Source: UniProtKB ubiquitin binding Inferred from sequence or structural similarity. Source: UniProtKB ubiquitin thiolesterase activity Inferred from sequence or structural similarity. Source: UniProtKB ubiquitin-specific protease activity Inferred from sequence or structural similarity. Source: UniProtKB zinc ion binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 901	901	Ubiquitin carboxyl-terminal hydrolase 16		PRO_0000367506
Regions
	Zinc finger	43 – 122	80	UBP-type
Sites
	Active site	206	1	Nucleophile By similarity
	Active site	835	1	Proton acceptor By similarity
	Metal binding	21	1	Zinc 1 By similarity
	Metal binding	23	1	Zinc 1 By similarity
	Metal binding	45	1	Zinc 2 By similarity
	Metal binding	48	1	Zinc 2 By similarity
	Metal binding	71	1	Zinc 3 By similarity
	Metal binding	74	1	Zinc 3 By similarity
	Metal binding	79	1	Zinc 2 By similarity
	Metal binding	87	1	Zinc 2 By similarity
	Metal binding	91	1	Zinc 3 By similarity
	Metal binding	100	1	Zinc 3 By similarity
	Metal binding	113	1	Zinc 1 By similarity
	Metal binding	116	1	Zinc 1 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6PAW2 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: EAC737894C997ABA	FASTA	901	101,293
	10 20 30 40 50 60 MVKKRGTNLP AQDFLDAEPV CKHLRKALDD GSVKRALVNV EWTMCQDCQA DNKEKNNSED 70 80 90 100 110 120 ESAEDPSVWL CLKCGHRGCG RNSVAQHALN HYNTPRSEPH CLVLNVDMWS AWCYLCDNEV 130 140 150 160 170 180 PYNRTSRLGQ LVDYLQRKAK AKNKNSNSVV SNEEVKTEIV TENEVKKIQY QDEKPDVQAK 190 200 210 220 230 240 QEKASSTQKI STEPTVKGLS NLGNTCFFNA VMQNLSQTPA LSELLNEVKT FRKPVTVLLP 250 260 270 280 290 300 DSSSPKILEV NLEQQPGPLT LAMWQFLTEM HETKKGVVTP KELFSQVCKK AIRFKGYQQQ 310 320 330 340 350 360 DSQELLRYLL DGMRGEEIQR VTLAMSKSLQ STLDEEEIKK IVKDYEKRRT IPNFVDCLFG 370 380 390 400 410 420 GELTSTIMCE ECHTVSLVHE PFLDLSLPVL DDLIVKKNSK STPPARERKE EEEEEENDDD 430 440 450 460 470 480 RYVKERDEVS PGASKHLQKK AKKAAKKQAK NQRRQQKWQG KTVLFTDLAK QECSEDEEEV 490 500 510 520 530 540 SQTKTNTRPD NETPTADGLN TMETDLSTLE NGNEESADGL NTMETDLSTL ENGNEEMAGG 550 560 570 580 590 600 FKTMEMDLST LENGSETIKS AVEGITEHTD LDSSVHNNVG SVETNALVGN MENNNNIEVN 610 620 630 640 650 660 KTPERTAGSG GDSMEAMAAV ENGNADAVNV DDTEAVNGLI DSANMDHELT NSLNRLQLSS 670 680 690 700 710 720 DLEPTQVEIE ILPDKEQPHT QVYEVVNEDP KTAFSTLSNR KDLPIDEFSV LSCLYQFTHK 730 740 750 760 770 780 ETLTGNNKLL CNVCTRKQAS RLNNSNKGEK KFVYTNAKKQ MLVSNPSPIL TLHLKRFQQN 790 800 810 820 830 840 GFNLRKINRH IKFPEVLDLA PFCTAKCKNV PEGESRLLYS LYGVIEHSGS MRSGHYTAFV 850 860 870 880 890 900 KLRHPNQQLC KMLFTGVIPE VSGSEPGQGS WYHISDSHVQ AVSLSRVLSS QAYLLFYERM L

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	NIH - Xenopus Gene Collection (XGC) project Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.
[2]	"Regulation of cell cycle progression and gene expression by H2A deubiquitination." Joo H.-Y., Zhai L., Yang C., Nie S., Erdjument-Bromage H., Tempst P., Chang C., Wang H. Nature 449:1068-1072(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC060022 mRNA. Translation: AAH60022.1.
RefSeq	NP_001083244.1. NM_001089775.1.
UniGene	Xl.32808.
3D structure databases
HSSP	HSSP built from PDB template 2GFO based on UniProtKB P40818.
ProteinModelPortal	Q6PAW2.
SMR	Q6PAW2. Positions 19-139.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	398823.
KEGG	xla:398823.
Organism-specific databases
CTD	10600.
Xenbase	XB-GENE-1007170. usp16.
Phylogenomic databases
HOVERGEN	HBG062704.
KO	K11844.
Family and domain databases
Gene3D	3.30.40.10. 1 hit.
InterPro	IPR018200. Pept_C19ubi-hydrolase_C_CS. IPR001394. Peptidase_C19. IPR013083. Znf_RING/FYVE/PHD. IPR001607. Znf_UBP. [Graphical view]
Pfam	PF00443. UCH. 1 hit. PF02148. zf-UBP. 1 hit. [Graphical view]
PROSITE	PS00972. UCH_2_1. 1 hit. PS00973. UCH_2_2. 1 hit. PS50235. UCH_2_3. 1 hit. PS50271. ZF_UBP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UBP16_XENLA

Accession

Primary (citable) accession number: Q6PAW2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	July 5, 2004
Last modified:	April 3, 2013
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program