Q6PAR5 (GAPD1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GTPase-activating protein and VPS9 domain-containing protein 1

Alternative name(s):
GAPex-5
Rab5-activating protein 6

Gene names

Name:	Gapvd1
Synonyms:	Gapex5, Kiaa1521

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	1458 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts both as a GTPase-activating protein (GAP) and a guanine nucleotide exchange factor (GEF), and participates in various processes such as endocytosis, insulin receptor internalization or LC2A4/GLUT4 trafficking. Acts as a GEF for the Ras-related protein RAB31 by exchanging bound GDP for free GTP, leading to regulate LC2A4/GLUT4 trafficking. In the absence of insulin, it maintains RAB31 in an active state and promotes a futile cycle between LC2A4/GLUT4 storage vesicles and early endosomes, retaining LC2A4/GLUT4 inside the cells. Upon insulin stimulation, it is translocated to the plasma membrane, releasing LC2A4/GLUT4 from intracellular storage vesicles. Also involved in EGFR trafficking and degradation, possibly by promoting EGFR ubiquitination and subsequent degradation by the proteasome. Has GEF activity for Rab5 and GAP activity for Ras. Ref.1 Ref.6 Ref.7
Subunit structure	Interacts with RAB5A By similarity. Interacts with TRIP10/CIP4. Ref.1
Subcellular location	Membrane; Peripheral membrane protein. Endosome By similarity. Note: Recruited to the plasma membrane by TRIP10/CIP4 in response to insulin. Ref.1
Tissue specificity	Present in adipocytes and fibroblasts (at protein level). Ubiquitously expressed. Ref.1
Sequence similarities	Belongs to the GAPVD1 family. Contains 1 Ras-GAP domain. Contains 1 VPS9 domain.
Sequence caution	The sequence AAH31478.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence. The sequence AAH43715.1 differs from that shown. Reason: Erroneous initiation. The sequence AAH48847.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence. The sequence AAH57164.1 differs from that shown. Reason: Erroneous initiation. The sequence BAB29377.2 differs from that shown. Reason: Erroneous initiation. The sequence BAC98191.1 differs from that shown. Reason: Erroneous initiation. The sequence BAE22277.1 differs from that shown. Reason: Erroneous initiation. The sequence BAE29251.1 differs from that shown. Reason: Frameshift at position 811. The sequence CAM15445.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAM15446.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAM15455.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAM15456.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAM24604.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAM24605.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Endocytosis
Cellular component	Endosome Membrane
Coding sequence diversity	Alternative splicing
Molecular function	GTPase activation Guanine-nucleotide releasing factor
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	endocytosis Inferred from electronic annotation. Source: UniProtKB-KW positive regulation of GTPase activity Inferred from electronic annotation. Source: GOC regulation of protein transport Inferred from mutant phenotype Ref.1. Source: UniProtKB regulation of small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro signal transduction Inferred from electronic annotation. Source: InterPro
Cellular_component	cytosol Inferred from direct assay Ref.1. Source: UniProtKB endosome Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	GTPase activating protein binding Inferred from mutant phenotype Ref.1. Source: UniProtKB GTPase activator activity Inferred from electronic annotation. Source: UniProtKB-KW guanyl-nucleotide exchange factor activity Inferred from mutant phenotype Ref.1. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 6 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q6PAR5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q6PAR5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     557-577: Missing.

Isoform 3 (identifier: Q6PAR5-3)

The sequence of this isoform differs from the canonical sequence as follows:
     373-379: GCVAAFL → VGMSVVS
     380-1458: Missing.

Isoform 4 (identifier: Q6PAR5-4)

The sequence of this isoform differs from the canonical sequence as follows:
     480-480: I → IGQQLAAITAWDSSATNLTAHIPLVTPF
     557-577: Missing.

Isoform 5 (identifier: Q6PAR5-5)

The sequence of this isoform differs from the canonical sequence as follows:
     557-577: Missing.
     1202-1202: Missing.

Isoform 6 (identifier: Q6PAR5-6)

The sequence of this isoform differs from the canonical sequence as follows:
     1055-1055: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1458	1458	GTPase-activating protein and VPS9 domain-containing protein 1		PRO_0000324772
Regions
	Domain	131 – 353	223	Ras-GAP
	Domain	1318 – 1458	141	VPS9
Amino acid modifications
	Modified residue	390	1	Phosphothreonine By similarity
	Modified residue	458	1	Phosphothreonine By similarity
	Modified residue	466	1	Phosphoserine By similarity
	Modified residue	470	1	Phosphothreonine By similarity
	Modified residue	566	1	Phosphoserine By similarity
	Modified residue	766	1	Phosphoserine By similarity
	Modified residue	902	1	Phosphoserine Ref.9
	Modified residue	903	1	Phosphoserine Ref.8
	Modified residue	964	1	Phosphoserine By similarity
	Modified residue	1017	1	Phosphoserine By similarity
	Modified residue	1076	1	Phosphoserine By similarity
	Modified residue	1085	1	Phosphoserine
Natural variations
	Alternative sequence	373 – 379	7	GCVAAFL → VGMSVVS in isoform 3.		VSP_032363
	Alternative sequence	380 – 1458	1079	Missing in isoform 3.		VSP_032364
	Alternative sequence	480	1	I → IGQQLAAITAWDSSATNLTA HIPLVTPF in isoform 4.		VSP_032365
	Alternative sequence	557 – 577	21	Missing in isoform 2, isoform 4 and isoform 5.		VSP_032366
	Alternative sequence	1055	1	Missing in isoform 6.		VSP_032367
	Alternative sequence	1202	1	Missing in isoform 5.		VSP_032368
Experimental info
	Sequence conflict	581	1	N → D in BAE29251. Ref.3
	Sequence conflict	661	1	G → E in AAH31478. Ref.5
	Sequence conflict	893	1	Y → H in BAE22277. Ref.3
	Sequence conflict	1257	1	A → V in BAC98191. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 18, 2008. Version 2. Checksum: CA3D2EAF22051FD7	FASTA	1,458	162,402
	10 20 30 40 50 60 MVKLDIHTLA HHLKQERLYV SSEKQLIQRL NADVLKTAEK LYRTAWIAKQ QRINLDRLII 70 80 90 100 110 120 TSAEASPAEC CQHAKILEDT QFVDGYKQLG FQETAYGEFL SRLRENPRLI ASSLVAGEKL 130 140 150 160 170 180 NQENTQSVIY TVFTSLYGNC IMQEDESYLL QVLRYLIEFE LKESDNPRRL LRRGTCAFSI 190 200 210 220 230 240 LFKLFSEGLF SAKLFLTATL HEPIMQLLVE DEDHLETDPN KLIERFSPAQ QEKLFGEKGS 250 260 270 280 290 300 DRFRQKVQEM VDSNEAKLVA LVNKFIGYLK QNTYCFPHSL RWIVSQMYKT LSCVDRLEVG 310 320 330 340 350 360 EVRAMCTDLL LACFICPAVV NPEQYGIISD APINEVARFN LMQVGRLLQQ LAMTGTEEGD 370 380 390 400 410 420 PRTKNSLGKF DKGCVAAFLD VVIGGRAVET PPMSSVNLLE GLSRTVVYIS YSQLITLVNF 430 440 450 460 470 480 MKSVMSGDQL KEDRMALDNL LANLPQAKPG KSSSLDMTPY STPQMSPATT PANKKNRLPI 490 500 510 520 530 540 ATRSRSRSNM LMDLHMDHEG SSQETIQEVQ PEEVLVISLG TGPQLTPGMM SENEVLNMQL 550 560 570 580 590 600 SDGGQGDVPV DENKLHGKPD KTLRFSLCSD NLEGISEGPS NRSNSVSSLD LEGESVSELG 610 620 630 640 650 660 AGPSGSNGVE ALQLLEHEQA TTQDNLDDKL RKFEIRDMMG LTDDRDISET VSETWSTDVL 670 680 690 700 710 720 GSDFDPNVDE DRLQEIAGAA AENVLGSLLC LPGSGSVLLD PCTGSTISET TSEAWSVEVL 730 740 750 760 770 780 PSDSEAPDLK QEERLQELES CSGLGSTSDD TDVREVSSRP STPGLSVVSG ISATSEDIPN 790 800 810 820 830 840 KIEDLRSECS SDFGGKDSVT SPDMDDIAHG AHQLTSPPSQ SESLLAMFDP LSSHEGASAV 850 860 870 880 890 900 VRPKVHYARP SHPPPDPPIL EGAVGGNEAR LPNFGSHVLT AAEMEAFKQR HSYPERLVRS 910 920 930 940 950 960 RSSDIVSSVR RPMSDPSWNR RPGNEELPPA AATGATSLVA APHSSSSSPS KDSSRGETEE 970 980 990 1000 1010 1020 RKDSDDERSD RSRPWWRKRF VSAMPKAPIP FRKKEKQEKD KDDLGPDRFS TLTDEPSPRL 1030 1040 1050 1060 1070 1080 SAQAQVAEDI LDKYRNAIKR TSPSEGAMAN DESAEVMGDG ESAHDSPREE ALQNISADDL 1090 1100 1110 1120 1130 1140 PDSASQAAHP QDSAFSYRDV KKKLRLALCS ADSVAFPVLT HSTRNGLPDH TDPEDNEIVC 1150 1160 1170 1180 1190 1200 FLKVQIAEAI NLQDKSLMAQ LQETMRCVCR FDNRTCRKLL ASIAEDYRKR APYIAYLTRC 1210 1220 1230 1240 1250 1260 RQGLQTTQAH LERLLQRVLR DKEVANRYFT TVCVRLLLES KEKKIREFIQ DFQKLTAADD 1270 1280 1290 1300 1310 1320 KTAQVEDFLQ FLYGVMAQDV IWQNASEEQL QDAQLAIERS VMNRIFKLAF YPNQDGDILR 1330 1340 1350 1360 1370 1380 DQVLHEHIQR LSKVVTANHR ALQIPEVYLR EAPWPSAQSE IRTISAYKTP RDKVQCILRM 1390 1400 1410 1420 1430 1440 CSTIMNLLSL ANEDSVPGAD DFVPVLVFVL IKANPPCLLS TVQYISSFYA SCLSGEESYW 1450 WMQFTAAVEF IKTIDDRK
	Isoform 2 [UniParc]. Checksum: 99CB3966DC97D605	FASTA	1,437	160,111
	10 20 30 40 50 60 MVKLDIHTLA HHLKQERLYV SSEKQLIQRL NADVLKTAEK LYRTAWIAKQ QRINLDRLII 70 80 90 100 110 120 TSAEASPAEC CQHAKILEDT QFVDGYKQLG FQETAYGEFL SRLRENPRLI ASSLVAGEKL 130 140 150 160 170 180 NQENTQSVIY TVFTSLYGNC IMQEDESYLL QVLRYLIEFE LKESDNPRRL LRRGTCAFSI 190 200 210 220 230 240 LFKLFSEGLF SAKLFLTATL HEPIMQLLVE DEDHLETDPN KLIERFSPAQ QEKLFGEKGS 250 260 270 280 290 300 DRFRQKVQEM VDSNEAKLVA LVNKFIGYLK QNTYCFPHSL RWIVSQMYKT LSCVDRLEVG 310 320 330 340 350 360 EVRAMCTDLL LACFICPAVV NPEQYGIISD APINEVARFN LMQVGRLLQQ LAMTGTEEGD 370 380 390 400 410 420 PRTKNSLGKF DKGCVAAFLD VVIGGRAVET PPMSSVNLLE GLSRTVVYIS YSQLITLVNF 430 440 450 460 470 480 MKSVMSGDQL KEDRMALDNL LANLPQAKPG KSSSLDMTPY STPQMSPATT PANKKNRLPI 490 500 510 520 530 540 ATRSRSRSNM LMDLHMDHEG SSQETIQEVQ PEEVLVISLG TGPQLTPGMM SENEVLNMQL 550 560 570 580 590 600 SDGGQGDVPV DENKLHGPSN RSNSVSSLDL EGESVSELGA GPSGSNGVEA LQLLEHEQAT 610 620 630 640 650 660 TQDNLDDKLR KFEIRDMMGL TDDRDISETV SETWSTDVLG SDFDPNVDED RLQEIAGAAA 670 680 690 700 710 720 ENVLGSLLCL PGSGSVLLDP CTGSTISETT SEAWSVEVLP SDSEAPDLKQ EERLQELESC 730 740 750 760 770 780 SGLGSTSDDT DVREVSSRPS TPGLSVVSGI SATSEDIPNK IEDLRSECSS DFGGKDSVTS 790 800 810 820 830 840 PDMDDIAHGA HQLTSPPSQS ESLLAMFDPL SSHEGASAVV RPKVHYARPS HPPPDPPILE 850 860 870 880 890 900 GAVGGNEARL PNFGSHVLTA AEMEAFKQRH SYPERLVRSR SSDIVSSVRR PMSDPSWNRR 910 920 930 940 950 960 PGNEELPPAA ATGATSLVAA PHSSSSSPSK DSSRGETEER KDSDDERSDR SRPWWRKRFV 970 980 990 1000 1010 1020 SAMPKAPIPF RKKEKQEKDK DDLGPDRFST LTDEPSPRLS AQAQVAEDIL DKYRNAIKRT 1030 1040 1050 1060 1070 1080 SPSEGAMAND ESAEVMGDGE SAHDSPREEA LQNISADDLP DSASQAAHPQ DSAFSYRDVK 1090 1100 1110 1120 1130 1140 KKLRLALCSA DSVAFPVLTH STRNGLPDHT DPEDNEIVCF LKVQIAEAIN LQDKSLMAQL 1150 1160 1170 1180 1190 1200 QETMRCVCRF DNRTCRKLLA SIAEDYRKRA PYIAYLTRCR QGLQTTQAHL ERLLQRVLRD 1210 1220 1230 1240 1250 1260 KEVANRYFTT VCVRLLLESK EKKIREFIQD FQKLTAADDK TAQVEDFLQF LYGVMAQDVI 1270 1280 1290 1300 1310 1320 WQNASEEQLQ DAQLAIERSV MNRIFKLAFY PNQDGDILRD QVLHEHIQRL SKVVTANHRA 1330 1340 1350 1360 1370 1380 LQIPEVYLRE APWPSAQSEI RTISAYKTPR DKVQCILRMC STIMNLLSLA NEDSVPGADD 1390 1400 1410 1420 1430 FVPVLVFVLI KANPPCLLST VQYISSFYAS CLSGEESYWW MQFTAAVEFI KTIDDRK
	Isoform 3 [UniParc]. Checksum: 7123D700EE30972C	FASTA	379	43,381
	10 20 30 40 50 60 MVKLDIHTLA HHLKQERLYV SSEKQLIQRL NADVLKTAEK LYRTAWIAKQ QRINLDRLII 70 80 90 100 110 120 TSAEASPAEC CQHAKILEDT QFVDGYKQLG FQETAYGEFL SRLRENPRLI ASSLVAGEKL 130 140 150 160 170 180 NQENTQSVIY TVFTSLYGNC IMQEDESYLL QVLRYLIEFE LKESDNPRRL LRRGTCAFSI 190 200 210 220 230 240 LFKLFSEGLF SAKLFLTATL HEPIMQLLVE DEDHLETDPN KLIERFSPAQ QEKLFGEKGS 250 260 270 280 290 300 DRFRQKVQEM VDSNEAKLVA LVNKFIGYLK QNTYCFPHSL RWIVSQMYKT LSCVDRLEVG 310 320 330 340 350 360 EVRAMCTDLL LACFICPAVV NPEQYGIISD APINEVARFN LMQVGRLLQQ LAMTGTEEGD 370 PRTKNSLGKF DKVGMSVVS
	Isoform 4 [UniParc]. Checksum: C34EEFDA11081BA7	FASTA	1,464	162,917
	10 20 30 40 50 60 MVKLDIHTLA HHLKQERLYV SSEKQLIQRL NADVLKTAEK LYRTAWIAKQ QRINLDRLII 70 80 90 100 110 120 TSAEASPAEC CQHAKILEDT QFVDGYKQLG FQETAYGEFL SRLRENPRLI ASSLVAGEKL 130 140 150 160 170 180 NQENTQSVIY TVFTSLYGNC IMQEDESYLL QVLRYLIEFE LKESDNPRRL LRRGTCAFSI 190 200 210 220 230 240 LFKLFSEGLF SAKLFLTATL HEPIMQLLVE DEDHLETDPN KLIERFSPAQ QEKLFGEKGS 250 260 270 280 290 300 DRFRQKVQEM VDSNEAKLVA LVNKFIGYLK QNTYCFPHSL RWIVSQMYKT LSCVDRLEVG 310 320 330 340 350 360 EVRAMCTDLL LACFICPAVV NPEQYGIISD APINEVARFN LMQVGRLLQQ LAMTGTEEGD 370 380 390 400 410 420 PRTKNSLGKF DKGCVAAFLD VVIGGRAVET PPMSSVNLLE GLSRTVVYIS YSQLITLVNF 430 440 450 460 470 480 MKSVMSGDQL KEDRMALDNL LANLPQAKPG KSSSLDMTPY STPQMSPATT PANKKNRLPI 490 500 510 520 530 540 GQQLAAITAW DSSATNLTAH IPLVTPFATR SRSRSNMLMD LHMDHEGSSQ ETIQEVQPEE 550 560 570 580 590 600 VLVISLGTGP QLTPGMMSEN EVLNMQLSDG GQGDVPVDEN KLHGPSNRSN SVSSLDLEGE 610 620 630 640 650 660 SVSELGAGPS GSNGVEALQL LEHEQATTQD NLDDKLRKFE IRDMMGLTDD RDISETVSET 670 680 690 700 710 720 WSTDVLGSDF DPNVDEDRLQ EIAGAAAENV LGSLLCLPGS GSVLLDPCTG STISETTSEA 730 740 750 760 770 780 WSVEVLPSDS EAPDLKQEER LQELESCSGL GSTSDDTDVR EVSSRPSTPG LSVVSGISAT 790 800 810 820 830 840 SEDIPNKIED LRSECSSDFG GKDSVTSPDM DDIAHGAHQL TSPPSQSESL LAMFDPLSSH 850 860 870 880 890 900 EGASAVVRPK VHYARPSHPP PDPPILEGAV GGNEARLPNF GSHVLTAAEM EAFKQRHSYP 910 920 930 940 950 960 ERLVRSRSSD IVSSVRRPMS DPSWNRRPGN EELPPAAATG ATSLVAAPHS SSSSPSKDSS 970 980 990 1000 1010 1020 RGETEERKDS DDERSDRSRP WWRKRFVSAM PKAPIPFRKK EKQEKDKDDL GPDRFSTLTD 1030 1040 1050 1060 1070 1080 EPSPRLSAQA QVAEDILDKY RNAIKRTSPS EGAMANDESA EVMGDGESAH DSPREEALQN 1090 1100 1110 1120 1130 1140 ISADDLPDSA SQAAHPQDSA FSYRDVKKKL RLALCSADSV AFPVLTHSTR NGLPDHTDPE 1150 1160 1170 1180 1190 1200 DNEIVCFLKV QIAEAINLQD KSLMAQLQET MRCVCRFDNR TCRKLLASIA EDYRKRAPYI 1210 1220 1230 1240 1250 1260 AYLTRCRQGL QTTQAHLERL LQRVLRDKEV ANRYFTTVCV RLLLESKEKK IREFIQDFQK 1270 1280 1290 1300 1310 1320 LTAADDKTAQ VEDFLQFLYG VMAQDVIWQN ASEEQLQDAQ LAIERSVMNR IFKLAFYPNQ 1330 1340 1350 1360 1370 1380 DGDILRDQVL HEHIQRLSKV VTANHRALQI PEVYLREAPW PSAQSEIRTI SAYKTPRDKV 1390 1400 1410 1420 1430 1440 QCILRMCSTI MNLLSLANED SVPGADDFVP VLVFVLIKAN PPCLLSTVQY ISSFYASCLS 1450 1460 GEESYWWMQF TAAVEFIKTI DDRK
	Isoform 5 [UniParc]. Checksum: 4367B45EA4C2D537	FASTA	1,436	159,982
	10 20 30 40 50 60 MVKLDIHTLA HHLKQERLYV SSEKQLIQRL NADVLKTAEK LYRTAWIAKQ QRINLDRLII 70 80 90 100 110 120 TSAEASPAEC CQHAKILEDT QFVDGYKQLG FQETAYGEFL SRLRENPRLI ASSLVAGEKL 130 140 150 160 170 180 NQENTQSVIY TVFTSLYGNC IMQEDESYLL QVLRYLIEFE LKESDNPRRL LRRGTCAFSI 190 200 210 220 230 240 LFKLFSEGLF SAKLFLTATL HEPIMQLLVE DEDHLETDPN KLIERFSPAQ QEKLFGEKGS 250 260 270 280 290 300 DRFRQKVQEM VDSNEAKLVA LVNKFIGYLK QNTYCFPHSL RWIVSQMYKT LSCVDRLEVG 310 320 330 340 350 360 EVRAMCTDLL LACFICPAVV NPEQYGIISD APINEVARFN LMQVGRLLQQ LAMTGTEEGD 370 380 390 400 410 420 PRTKNSLGKF DKGCVAAFLD VVIGGRAVET PPMSSVNLLE GLSRTVVYIS YSQLITLVNF 430 440 450 460 470 480 MKSVMSGDQL KEDRMALDNL LANLPQAKPG KSSSLDMTPY STPQMSPATT PANKKNRLPI 490 500 510 520 530 540 ATRSRSRSNM LMDLHMDHEG SSQETIQEVQ PEEVLVISLG TGPQLTPGMM SENEVLNMQL 550 560 570 580 590 600 SDGGQGDVPV DENKLHGPSN RSNSVSSLDL EGESVSELGA GPSGSNGVEA LQLLEHEQAT 610 620 630 640 650 660 TQDNLDDKLR KFEIRDMMGL TDDRDISETV SETWSTDVLG SDFDPNVDED RLQEIAGAAA 670 680 690 700 710 720 ENVLGSLLCL PGSGSVLLDP CTGSTISETT SEAWSVEVLP SDSEAPDLKQ EERLQELESC 730 740 750 760 770 780 SGLGSTSDDT DVREVSSRPS TPGLSVVSGI SATSEDIPNK IEDLRSECSS DFGGKDSVTS 790 800 810 820 830 840 PDMDDIAHGA HQLTSPPSQS ESLLAMFDPL SSHEGASAVV RPKVHYARPS HPPPDPPILE 850 860 870 880 890 900 GAVGGNEARL PNFGSHVLTA AEMEAFKQRH SYPERLVRSR SSDIVSSVRR PMSDPSWNRR 910 920 930 940 950 960 PGNEELPPAA ATGATSLVAA PHSSSSSPSK DSSRGETEER KDSDDERSDR SRPWWRKRFV 970 980 990 1000 1010 1020 SAMPKAPIPF RKKEKQEKDK DDLGPDRFST LTDEPSPRLS AQAQVAEDIL DKYRNAIKRT 1030 1040 1050 1060 1070 1080 SPSEGAMAND ESAEVMGDGE SAHDSPREEA LQNISADDLP DSASQAAHPQ DSAFSYRDVK 1090 1100 1110 1120 1130 1140 KKLRLALCSA DSVAFPVLTH STRNGLPDHT DPEDNEIVCF LKVQIAEAIN LQDKSLMAQL 1150 1160 1170 1180 1190 1200 QETMRCVCRF DNRTCRKLLA SIAEDYRKRA PYIAYLTRCR GLQTTQAHLE RLLQRVLRDK 1210 1220 1230 1240 1250 1260 EVANRYFTTV CVRLLLESKE KKIREFIQDF QKLTAADDKT AQVEDFLQFL YGVMAQDVIW 1270 1280 1290 1300 1310 1320 QNASEEQLQD AQLAIERSVM NRIFKLAFYP NQDGDILRDQ VLHEHIQRLS KVVTANHRAL 1330 1340 1350 1360 1370 1380 QIPEVYLREA PWPSAQSEIR TISAYKTPRD KVQCILRMCS TIMNLLSLAN EDSVPGADDF 1390 1400 1410 1420 1430 VPVLVFVLIK ANPPCLLSTV QYISSFYASC LSGEESYWWM QFTAAVEFIK TIDDRK
	Isoform 6 [UniParc]. Checksum: 67DC7B5E4E46334D	FASTA	1,457	162,273
	10 20 30 40 50 60 MVKLDIHTLA HHLKQERLYV SSEKQLIQRL NADVLKTAEK LYRTAWIAKQ QRINLDRLII 70 80 90 100 110 120 TSAEASPAEC CQHAKILEDT QFVDGYKQLG FQETAYGEFL SRLRENPRLI ASSLVAGEKL 130 140 150 160 170 180 NQENTQSVIY TVFTSLYGNC IMQEDESYLL QVLRYLIEFE LKESDNPRRL LRRGTCAFSI 190 200 210 220 230 240 LFKLFSEGLF SAKLFLTATL HEPIMQLLVE DEDHLETDPN KLIERFSPAQ QEKLFGEKGS 250 260 270 280 290 300 DRFRQKVQEM VDSNEAKLVA LVNKFIGYLK QNTYCFPHSL RWIVSQMYKT LSCVDRLEVG 310 320 330 340 350 360 EVRAMCTDLL LACFICPAVV NPEQYGIISD APINEVARFN LMQVGRLLQQ LAMTGTEEGD 370 380 390 400 410 420 PRTKNSLGKF DKGCVAAFLD VVIGGRAVET PPMSSVNLLE GLSRTVVYIS YSQLITLVNF 430 440 450 460 470 480 MKSVMSGDQL KEDRMALDNL LANLPQAKPG KSSSLDMTPY STPQMSPATT PANKKNRLPI 490 500 510 520 530 540 ATRSRSRSNM LMDLHMDHEG SSQETIQEVQ PEEVLVISLG TGPQLTPGMM SENEVLNMQL 550 560 570 580 590 600 SDGGQGDVPV DENKLHGKPD KTLRFSLCSD NLEGISEGPS NRSNSVSSLD LEGESVSELG 610 620 630 640 650 660 AGPSGSNGVE ALQLLEHEQA TTQDNLDDKL RKFEIRDMMG LTDDRDISET VSETWSTDVL 670 680 690 700 710 720 GSDFDPNVDE DRLQEIAGAA AENVLGSLLC LPGSGSVLLD PCTGSTISET TSEAWSVEVL 730 740 750 760 770 780 PSDSEAPDLK QEERLQELES CSGLGSTSDD TDVREVSSRP STPGLSVVSG ISATSEDIPN 790 800 810 820 830 840 KIEDLRSECS SDFGGKDSVT SPDMDDIAHG AHQLTSPPSQ SESLLAMFDP LSSHEGASAV 850 860 870 880 890 900 VRPKVHYARP SHPPPDPPIL EGAVGGNEAR LPNFGSHVLT AAEMEAFKQR HSYPERLVRS 910 920 930 940 950 960 RSSDIVSSVR RPMSDPSWNR RPGNEELPPA AATGATSLVA APHSSSSSPS KDSSRGETEE 970 980 990 1000 1010 1020 RKDSDDERSD RSRPWWRKRF VSAMPKAPIP FRKKEKQEKD KDDLGPDRFS TLTDEPSPRL 1030 1040 1050 1060 1070 1080 SAQAQVAEDI LDKYRNAIKR TSPSEGAMAN DESAVMGDGE SAHDSPREEA LQNISADDLP 1090 1100 1110 1120 1130 1140 DSASQAAHPQ DSAFSYRDVK KKLRLALCSA DSVAFPVLTH STRNGLPDHT DPEDNEIVCF 1150 1160 1170 1180 1190 1200 LKVQIAEAIN LQDKSLMAQL QETMRCVCRF DNRTCRKLLA SIAEDYRKRA PYIAYLTRCR 1210 1220 1230 1240 1250 1260 QGLQTTQAHL ERLLQRVLRD KEVANRYFTT VCVRLLLESK EKKIREFIQD FQKLTAADDK 1270 1280 1290 1300 1310 1320 TAQVEDFLQF LYGVMAQDVI WQNASEEQLQ DAQLAIERSV MNRIFKLAFY PNQDGDILRD 1330 1340 1350 1360 1370 1380 QVLHEHIQRL SKVVTANHRA LQIPEVYLRE APWPSAQSEI RTISAYKTPR DKVQCILRMC 1390 1400 1410 1420 1430 1440 STIMNLLSLA NEDSVPGADD FVPVLVFVLI KANPPCLLST VQYISSFYAS CLSGEESYWW 1450 MQFTAAVEFI KTIDDRK

References

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[1]	"Gapex-5, a Rab31 guanine nucleotide exchange factor that regulates Glut4 trafficking in adipocytes." Lodhi I.J., Chiang S.-H., Chang L., Vollenweider D., Watson R.T., Inoue M., Pessin J.E., Saltiel A.R. Cell Metab. 5:59-72(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH TRIP10. Strain: Swiss albino.
[2]	"Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries." Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H. DNA Res. 10:167-180(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 5).
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 3 AND 4), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 973-1458 (ISOFORM 6). Strain: C57BL/6J and NOD. Tissue: Eye, Liver, Medulla oblongata and Thymus.
[4]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: C57BL/6, FVB/N and FVB/N-3. Tissue: Brain, Kidney and Mammary tumor.
[6]	"Insulin-stimulated Interaction between insulin receptor substrate 1 and p85alpha and activation of protein kinase B/Akt require Rab5." Su X., Lodhi I.J., Saltiel A.R., Stahl P.D. J. Biol. Chem. 281:27982-27990(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[7]	"GAPex-5 mediates ubiquitination, trafficking, and degradation of epidermal growth factor receptor." Su X., Kong C., Stahl P.D. J. Biol. Chem. 282:21278-21284(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[8]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-903 AND 1085, MASS SPECTROMETRY. Tissue: Liver.
[9]	"Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry." Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J. Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-902, MASS SPECTROMETRY. Tissue: Embryonic fibroblast.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	EF155419 mRNA. Translation: ABM68541.1. AK129381 Transcribed RNA. Translation: BAC98191.1. Different initiation. AK014474 mRNA. Translation: BAB29377.2. Different initiation. AK088851 mRNA. Translation: BAC40613.1. AK003521 mRNA. Translation: BAB22834.1. AK134776 mRNA. Translation: BAE22277.1. Different initiation. AK150026 mRNA. Translation: BAE29251.1. Frameshift. AK165047 mRNA. Translation: BAE38017.1. AL845262, AL929106 Genomic DNA. Translation: CAM15445.1. Sequence problems. AL845262, AL929106 Genomic DNA. Translation: CAM15446.1. Sequence problems. AL845262, AL929106 Genomic DNA. Translation: CAM15447.1. AL845262 Genomic DNA. Translation: CAM15455.1. Sequence problems. AL845262 Genomic DNA. Translation: CAM15456.1. Sequence problems. AL929106, AL845262 Genomic DNA. Translation: CAM24604.1. Sequence problems. AL929106, AL845262 Genomic DNA. Translation: CAM24605.1. Sequence problems. AL929106, AL845262 Genomic DNA. Translation: CAM24606.1. BC031478 mRNA. Translation: AAH31478.1. Sequence problems. BC043715 mRNA. Translation: AAH43715.1. Different initiation. BC048847 mRNA. Translation: AAH48847.1. Sequence problems. BC057164 mRNA. Translation: AAH57164.1. Different initiation. BC060123 mRNA. Translation: AAH60123.1.
IPI	IPI00406596. IPI00889216. IPI00889225. IPI00889231. IPI00889244. IPI00889253.
RefSeq	NP_079985.2. NM_025709.2.
UniGene	Mm.156452. Mm.393397.
3D structure databases
ProteinModelPortal	Q6PAR5.
SMR	Q6PAR5. Positions 1243-1457.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-4125168.
PTM databases
PhosphoSite	Q6PAR5.
Proteomic databases
PaxDb	Q6PAR5.
PRIDE	Q6PAR5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000028224; ENSMUSP00000028224; ENSMUSG00000026867. ENSMUST00000102800; ENSMUSP00000099864; ENSMUSG00000026867. ENSMUST00000113099; ENSMUSP00000108723; ENSMUSG00000026867.
GeneID	66691.
KEGG	mmu:66691.
UCSC	uc008jiq.2. mouse. uc008jir.1. mouse. uc012bug.1. mouse.
Organism-specific databases
CTD	26130.
MGI	MGI:1913941. Gapvd1.
Rouge	Search...
Phylogenomic databases
eggNOG	NOG275317.
GeneTree	ENSGT00530000063341.
HOVERGEN	HBG107936.
InParanoid	Q6PAR5.
OMA	HSYPERL.
OrthoDB	EOG4548XR.
Gene expression databases
ArrayExpress	Q6PAR5.
Bgee	Q6PAR5.
CleanEx	MM_GAPVD1.
Genevestigator	Q6PAR5.
Family and domain databases
Gene3D	1.10.506.10. 1 hit.
InterPro	IPR001936. RasGAP. IPR008936. Rho_GTPase_activation_prot. IPR003123. VPS9. IPR013995. VPS9_subgr. [Graphical view]
Pfam	PF00616. RasGAP. 1 hit. PF02204. VPS9. 1 hit. [Graphical view]
SMART	SM00167. VPS9. 1 hit. [Graphical view]
SUPFAM	SSF48350. Rho_GAP. 1 hit.
PROSITE	PS00509. RAS_GTPASE_ACTIV_1. False negative. PS50018. RAS_GTPASE_ACTIV_2. 1 hit. PS51205. VPS9. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	GAPVD1. mouse.
NextBio	322389.
SOURCE	Search...

Entry information

Entry name

GAPD1_MOUSE

Accession

Primary (citable) accession number: Q6PAR5
Secondary accession number(s): A0PJI8 , A2AR09, A2AR10, A2AR17, A2AR18, Q3TNS1, Q3UDL0, Q3UYD5, Q6ZPP0, Q80V37, Q80ZK4, Q8BTS5, Q9CRS2, Q9CTI1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	March 18, 2008
Last modified:	May 29, 2013
This is version 76 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program