Q6PAM0 (AAKB2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5'-AMP-activated protein kinase subunit beta-2
Short name=AMPK subunit beta-2

Gene names

Name:

Prkab2

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	271 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3) By similarity.
Subunit structure	AMPK is a heterotrimer of an alpha catalytic subunit (PRKAA1 or PRKAA2), a beta (PRKAB1 or PRKAB2) and a gamma non-catalytic subunits (PRKAG1, PRKAG2 or PRKAG3) By similarity.
Post-translational modification	Phosphorylated when associated with the catalytic subunit (PRKAA1 or PRKAA2). Phosphorylated by ULK1 and ULK2; leading to negatively regulate AMPK activity and suggesting the existence of a regulatory feedback loop between ULK1, ULK2 and AMPK. Ref.2
Sequence similarities	Belongs to the 5'-AMP-activated protein kinase beta subunit family.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Fatty acid metabolism Lipid biosynthesis Lipid metabolism
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	fatty acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW response to stress Traceable author statement PubMed 10098881. Source: MGI
Cellular_component	AMP-activated protein kinase complex Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	AMP-activated protein kinase activity Traceable author statement PubMed 10098881. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 271	271	5'-AMP-activated protein kinase subunit beta-2		PRO_0000204369
Amino acid modifications
	Modified residue	38	1	Phosphoserine; by ULK1 By similarity
	Modified residue	39	1	Phosphothreonine; by ULK1 By similarity
	Modified residue	68	1	Phosphoserine; by ULK1 By similarity
	Modified residue	107	1	Phosphoserine By similarity
	Modified residue	173	1	Phosphoserine By similarity
	Modified residue	183	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6PAM0 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 883B42716E996BE7	FASTA	271	30,209
	10 20 30 40 50 60 MGNTTSERVS GERHGAKAAR AEGGGHGPGK EHKIMVGSTD DPSVFSLPDS KLPGDKEFVP 70 80 90 100 110 120 WQQDLDDSVK PAQQARPTVI RWSEGGKEVF ISGSFNNWST KIPLIKSHND FVAILDLPEG 130 140 150 160 170 180 EHQYKFFVDG QWVHDPSEPV VTSQLGTINN LIHVKKSDFE VFDALKLDSM ESSETSCRDL 190 200 210 220 230 240 SSSPPGPYGQ EMYVFRSEER FKSPPILPPH LLQVILNKDT NISCDPALLP EPNHVMLNHL 250 260 270 YALSIKDSVM VLSATHRYKK KYVTTLLYKP I

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Brain.
[2]	"Ulk1-mediated phosphorylation of AMPK constitutes a negative regulatory feedback loop." Loffler A.S., Alers S., Dieterle A.M., Keppeler H., Franz-Wachtel M., Kundu M., Campbell D.G., Wesselborg S., Alessi D.R., Stork B. Autophagy 7:696-706(2011) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION BY ULK1 AND ULK2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC060228 mRNA. Translation: AAH60228.1.
IPI	IPI00340519.
RefSeq	NP_892042.2. NM_182997.2.
UniGene	Mm.31175.
3D structure databases
ProteinModelPortal	Q6PAM0.
SMR	Q6PAM0. Positions 74-271.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000036410.
Protein family/group databases
CAZy	CBM48. Carbohydrate-Binding Module Family 48.
PTM databases
PhosphoSite	Q6PAM0.
Proteomic databases
PaxDb	Q6PAM0.
PRIDE	Q6PAM0.
Protocols and materials databases
DNASU	108097.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000045743; ENSMUSP00000036410; ENSMUSG00000038205.
GeneID	108097.
KEGG	mmu:108097.
UCSC	uc008qpb.1. mouse.
Organism-specific databases
CTD	5565.
MGI	MGI:1336185. Prkab2.
Phylogenomic databases
eggNOG	NOG238368.
GeneTree	ENSGT00390000001416.
HOGENOM	HOG000230597.
HOVERGEN	HBG050430.
InParanoid	Q6PAM0.
KO	K07199.
OMA	SGIYHYK.
OrthoDB	EOG4W9J4H.
Enzyme and pathway databases
Reactome	REACT_147847. Translocation of Glut4 to the Plasma Membrane. REACT_88307. Membrane Trafficking.
Gene expression databases
ArrayExpress	Q6PAM0.
Bgee	Q6PAM0.
Genevestigator	Q6PAM0.
GermOnline	ENSMUSG00000038205. Mus musculus.
Family and domain databases
InterPro	IPR006828. AMP_prot_kin_bsu_interact-dom. [Graphical view]
Pfam	PF04739. AMPKBI. 1 hit. [Graphical view]
SMART	SM01010. AMPKBI. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	PRKAB2. mouse.
NextBio	360054.
SOURCE	Search...

Entry information

Entry name

AAKB2_MOUSE

Accession

Primary (citable) accession number: Q6PAM0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 12, 2005
Last sequence update:	July 5, 2004
Last modified:	April 3, 2013
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program