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Protein

5'-AMP-activated protein kinase subunit beta-2

Gene

Prkab2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3) (By similarity).By similarity

GO - Molecular functioni

  1. AMP-activated protein kinase activity Source: MGI
  2. identical protein binding Source: MGI

GO - Biological processi

  1. fatty acid biosynthetic process Source: UniProtKB-KW
  2. protein phosphorylation Source: MGI
  3. regulation of protein kinase activity Source: InterPro
  4. signal transduction Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

Enzyme and pathway databases

ReactomeiREACT_273617. AMPK inhibits chREBP transcriptional activation activity.
REACT_288513. Regulation of AMPK activity via LKB1.
REACT_293784. Import of palmitoyl-CoA into the mitochondrial matrix.
REACT_302653. Translocation of GLUT4 to the plasma membrane.
REACT_307891. Regulation of Rheb GTPase activity by AMPK.
REACT_321345. Activation of PPARGC1A (PGC-1alpha) by phosphorylation.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.

Names & Taxonomyi

Protein namesi
Recommended name:
5'-AMP-activated protein kinase subunit beta-2
Short name:
AMPK subunit beta-2
Gene namesi
Name:Prkab2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1336185. Prkab2.

Subcellular locationi

GO - Cellular componenti

  1. AMP-activated protein kinase complex Source: UniProtKB
  2. cytosol Source: Reactome
  3. nucleoplasm Source: Reactome
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 2712715'-AMP-activated protein kinase subunit beta-2PRO_0000204369Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei38 – 381Phosphoserine; by ULK1By similarity
Modified residuei39 – 391Phosphothreonine; by ULK1By similarity
Modified residuei68 – 681Phosphoserine; by ULK1By similarity
Modified residuei94 – 941PhosphoserineBy similarity
Modified residuei107 – 1071PhosphoserineBy similarity
Modified residuei147 – 1471PhosphothreonineBy similarity
Modified residuei157 – 1571PhosphoserineBy similarity
Modified residuei169 – 1691PhosphoserineBy similarity
Modified residuei173 – 1731PhosphoserineBy similarity
Modified residuei183 – 1831PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated when associated with the catalytic subunit (PRKAA1 or PRKAA2). Phosphorylated by ULK1 and ULK2; leading to negatively regulate AMPK activity and suggesting the existence of a regulatory feedback loop between ULK1, ULK2 and AMPK.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ6PAM0.
PaxDbiQ6PAM0.
PRIDEiQ6PAM0.

PTM databases

PhosphoSiteiQ6PAM0.

Expressioni

Gene expression databases

BgeeiQ6PAM0.
ExpressionAtlasiQ6PAM0. baseline and differential.
GenevestigatoriQ6PAM0.

Interactioni

Subunit structurei

AMPK is a heterotrimer of an alpha catalytic subunit (PRKAA1 or PRKAA2), a beta (PRKAB1 or PRKAB2) and a gamma non-catalytic subunits (PRKAG1, PRKAG2 or PRKAG3).By similarity

Protein-protein interaction databases

IntActiQ6PAM0. 1 interaction.
MINTiMINT-4086434.
STRINGi10090.ENSMUSP00000036410.

Structurei

3D structure databases

ProteinModelPortaliQ6PAM0.
SMRiQ6PAM0. Positions 74-271.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG238368.
GeneTreeiENSGT00390000001416.
HOGENOMiHOG000230597.
HOVERGENiHBG050430.
InParanoidiQ6PAM0.
KOiK07199.
OMAiESKYITV.
PhylomeDBiQ6PAM0.
TreeFamiTF313827.

Family and domain databases

InterProiIPR030081. AMPK_beta-2.
IPR006828. ASC_dom.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10343:SF43. PTHR10343:SF43. 1 hit.
PfamiPF04739. AMPKBI. 1 hit.
[Graphical view]
SMARTiSM01010. AMPKBI. 1 hit.
[Graphical view]
SUPFAMiSSF81296. SSF81296. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6PAM0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGNTTSERVS GERHGAKAAR AEGGGHGPGK EHKIMVGSTD DPSVFSLPDS
60 70 80 90 100
KLPGDKEFVP WQQDLDDSVK PAQQARPTVI RWSEGGKEVF ISGSFNNWST
110 120 130 140 150
KIPLIKSHND FVAILDLPEG EHQYKFFVDG QWVHDPSEPV VTSQLGTINN
160 170 180 190 200
LIHVKKSDFE VFDALKLDSM ESSETSCRDL SSSPPGPYGQ EMYVFRSEER
210 220 230 240 250
FKSPPILPPH LLQVILNKDT NISCDPALLP EPNHVMLNHL YALSIKDSVM
260 270
VLSATHRYKK KYVTTLLYKP I
Length:271
Mass (Da):30,209
Last modified:July 5, 2004 - v1
Checksum:i883B42716E996BE7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC060228 mRNA. Translation: AAH60228.1.
CCDSiCCDS17657.1.
RefSeqiNP_892042.2. NM_182997.2.
XP_006500903.1. XM_006500840.1.
UniGeneiMm.31175.

Genome annotation databases

EnsembliENSMUST00000045743; ENSMUSP00000036410; ENSMUSG00000038205.
GeneIDi108097.
KEGGimmu:108097.
UCSCiuc008qpb.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC060228 mRNA. Translation: AAH60228.1.
CCDSiCCDS17657.1.
RefSeqiNP_892042.2. NM_182997.2.
XP_006500903.1. XM_006500840.1.
UniGeneiMm.31175.

3D structure databases

ProteinModelPortaliQ6PAM0.
SMRiQ6PAM0. Positions 74-271.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ6PAM0. 1 interaction.
MINTiMINT-4086434.
STRINGi10090.ENSMUSP00000036410.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.

PTM databases

PhosphoSiteiQ6PAM0.

Proteomic databases

MaxQBiQ6PAM0.
PaxDbiQ6PAM0.
PRIDEiQ6PAM0.

Protocols and materials databases

DNASUi108097.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000045743; ENSMUSP00000036410; ENSMUSG00000038205.
GeneIDi108097.
KEGGimmu:108097.
UCSCiuc008qpb.1. mouse.

Organism-specific databases

CTDi5565.
MGIiMGI:1336185. Prkab2.

Phylogenomic databases

eggNOGiNOG238368.
GeneTreeiENSGT00390000001416.
HOGENOMiHOG000230597.
HOVERGENiHBG050430.
InParanoidiQ6PAM0.
KOiK07199.
OMAiESKYITV.
PhylomeDBiQ6PAM0.
TreeFamiTF313827.

Enzyme and pathway databases

ReactomeiREACT_273617. AMPK inhibits chREBP transcriptional activation activity.
REACT_288513. Regulation of AMPK activity via LKB1.
REACT_293784. Import of palmitoyl-CoA into the mitochondrial matrix.
REACT_302653. Translocation of GLUT4 to the plasma membrane.
REACT_307891. Regulation of Rheb GTPase activity by AMPK.
REACT_321345. Activation of PPARGC1A (PGC-1alpha) by phosphorylation.

Miscellaneous databases

ChiTaRSiPrkab2. mouse.
NextBioi360054.
PROiQ6PAM0.
SOURCEiSearch...

Gene expression databases

BgeeiQ6PAM0.
ExpressionAtlasiQ6PAM0. baseline and differential.
GenevestigatoriQ6PAM0.

Family and domain databases

InterProiIPR030081. AMPK_beta-2.
IPR006828. ASC_dom.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10343:SF43. PTHR10343:SF43. 1 hit.
PfamiPF04739. AMPKBI. 1 hit.
[Graphical view]
SMARTiSM01010. AMPKBI. 1 hit.
[Graphical view]
SUPFAMiSSF81296. SSF81296. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain.
  2. "Ulk1-mediated phosphorylation of AMPK constitutes a negative regulatory feedback loop."
    Loffler A.S., Alers S., Dieterle A.M., Keppeler H., Franz-Wachtel M., Kundu M., Campbell D.G., Wesselborg S., Alessi D.R., Stork B.
    Autophagy 7:696-706(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION BY ULK1 AND ULK2.

Entry informationi

Entry nameiAAKB2_MOUSE
AccessioniPrimary (citable) accession number: Q6PAM0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 12, 2005
Last sequence update: July 5, 2004
Last modified: April 1, 2015
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.