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Q6PAM0 (AAKB2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
5'-AMP-activated protein kinase subunit beta-2

Short name=AMPK subunit beta-2
Gene names
Name:Prkab2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length271 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3) By similarity.

Subunit structure

AMPK is a heterotrimer of an alpha catalytic subunit (PRKAA1 or PRKAA2), a beta (PRKAB1 or PRKAB2) and a gamma non-catalytic subunits (PRKAG1, PRKAG2 or PRKAG3) By similarity.

Post-translational modification

Phosphorylated when associated with the catalytic subunit (PRKAA1 or PRKAA2). Phosphorylated by ULK1 and ULK2; leading to negatively regulate AMPK activity and suggesting the existence of a regulatory feedback loop between ULK1, ULK2 and AMPK. Ref.2

Sequence similarities

Belongs to the 5'-AMP-activated protein kinase beta subunit family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 2712715'-AMP-activated protein kinase subunit beta-2
PRO_0000204369

Amino acid modifications

Modified residue381Phosphoserine; by ULK1 By similarity
Modified residue391Phosphothreonine; by ULK1 By similarity
Modified residue681Phosphoserine; by ULK1 By similarity
Modified residue1071Phosphoserine By similarity
Modified residue1731Phosphoserine By similarity
Modified residue1831Phosphoserine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q6PAM0 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 883B42716E996BE7

FASTA27130,209
        10         20         30         40         50         60 
MGNTTSERVS GERHGAKAAR AEGGGHGPGK EHKIMVGSTD DPSVFSLPDS KLPGDKEFVP 

        70         80         90        100        110        120 
WQQDLDDSVK PAQQARPTVI RWSEGGKEVF ISGSFNNWST KIPLIKSHND FVAILDLPEG 

       130        140        150        160        170        180 
EHQYKFFVDG QWVHDPSEPV VTSQLGTINN LIHVKKSDFE VFDALKLDSM ESSETSCRDL 

       190        200        210        220        230        240 
SSSPPGPYGQ EMYVFRSEER FKSPPILPPH LLQVILNKDT NISCDPALLP EPNHVMLNHL 

       250        260        270 
YALSIKDSVM VLSATHRYKK KYVTTLLYKP I 

« Hide

References

« Hide 'large scale' references
[1]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.
Tissue: Brain.
[2]"Ulk1-mediated phosphorylation of AMPK constitutes a negative regulatory feedback loop."
Loffler A.S., Alers S., Dieterle A.M., Keppeler H., Franz-Wachtel M., Kundu M., Campbell D.G., Wesselborg S., Alessi D.R., Stork B.
Autophagy 7:696-706(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION BY ULK1 AND ULK2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC060228 mRNA. Translation: AAH60228.1.
RefSeqNP_892042.2. NM_182997.2.
XP_006500903.1. XM_006500840.1.
UniGeneMm.31175.

3D structure databases

ProteinModelPortalQ6PAM0.
SMRQ6PAM0. Positions 74-271.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ6PAM0. 1 interaction.
MINTMINT-4086434.
STRING10090.ENSMUSP00000036410.

Protein family/group databases

CAZyCBM48. Carbohydrate-Binding Module Family 48.

PTM databases

PhosphoSiteQ6PAM0.

Proteomic databases

PaxDbQ6PAM0.
PRIDEQ6PAM0.

Protocols and materials databases

DNASU108097.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000045743; ENSMUSP00000036410; ENSMUSG00000038205.
GeneID108097.
KEGGmmu:108097.
UCSCuc008qpb.1. mouse.

Organism-specific databases

CTD5565.
MGIMGI:1336185. Prkab2.

Phylogenomic databases

eggNOGNOG238368.
GeneTreeENSGT00390000001416.
HOGENOMHOG000230597.
HOVERGENHBG050430.
InParanoidQ6PAM0.
KOK07199.
OMASGIYHYK.
PhylomeDBQ6PAM0.
TreeFamTF313827.

Enzyme and pathway databases

ReactomeREACT_147847. Translocation of Glut4 to the Plasma Membrane.

Gene expression databases

ArrayExpressQ6PAM0.
BgeeQ6PAM0.
GenevestigatorQ6PAM0.

Family and domain databases

InterProIPR006828. AMP_prot_kin_bsu_interact-dom.
IPR014756. Ig_E-set.
[Graphical view]
PfamPF04739. AMPKBI. 1 hit.
[Graphical view]
SMARTSM01010. AMPKBI. 1 hit.
[Graphical view]
SUPFAMSSF81296. SSF81296. 1 hit.
ProtoNetSearch...

Other

ChiTaRSPRKAB2. mouse.
NextBio360054.
PROQ6PAM0.
SOURCESearch...

Entry information

Entry nameAAKB2_MOUSE
AccessionPrimary (citable) accession number: Q6PAM0
Entry history
Integrated into UniProtKB/Swiss-Prot: April 12, 2005
Last sequence update: July 5, 2004
Last modified: April 16, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot