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Q6PAM0

- AAKB2_MOUSE

UniProt

Q6PAM0 - AAKB2_MOUSE

Protein

5'-AMP-activated protein kinase subunit beta-2

Gene

Prkab2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 80 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3) By similarity.By similarity

    GO - Molecular functioni

    1. AMP-activated protein kinase activity Source: MGI

    GO - Biological processi

    1. fatty acid biosynthetic process Source: UniProtKB-KW
    2. protein phosphorylation Source: GOC

    Keywords - Biological processi

    Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

    Enzyme and pathway databases

    ReactomeiREACT_199054. Translocation of GLUT4 to the plasma membrane.
    REACT_205688. Activation of PPARGC1A (PGC-1alpha) by phosphorylation.
    REACT_220701. Regulation of AMPK activity via LKB1.

    Protein family/group databases

    CAZyiCBM48. Carbohydrate-Binding Module Family 48.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    5'-AMP-activated protein kinase subunit beta-2
    Short name:
    AMPK subunit beta-2
    Gene namesi
    Name:Prkab2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 3

    Organism-specific databases

    MGIiMGI:1336185. Prkab2.

    Subcellular locationi

    GO - Cellular componenti

    1. AMP-activated protein kinase complex Source: UniProtKB
    2. cytosol Source: Reactome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 2712715'-AMP-activated protein kinase subunit beta-2PRO_0000204369Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei38 – 381Phosphoserine; by ULK1By similarity
    Modified residuei39 – 391Phosphothreonine; by ULK1By similarity
    Modified residuei68 – 681Phosphoserine; by ULK1By similarity
    Modified residuei107 – 1071PhosphoserineBy similarity
    Modified residuei173 – 1731PhosphoserineBy similarity
    Modified residuei183 – 1831PhosphoserineBy similarity

    Post-translational modificationi

    Phosphorylated when associated with the catalytic subunit (PRKAA1 or PRKAA2). Phosphorylated by ULK1 and ULK2; leading to negatively regulate AMPK activity and suggesting the existence of a regulatory feedback loop between ULK1, ULK2 and AMPK.1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ6PAM0.
    PaxDbiQ6PAM0.
    PRIDEiQ6PAM0.

    PTM databases

    PhosphoSiteiQ6PAM0.

    Expressioni

    Gene expression databases

    ArrayExpressiQ6PAM0.
    BgeeiQ6PAM0.
    GenevestigatoriQ6PAM0.

    Interactioni

    Subunit structurei

    AMPK is a heterotrimer of an alpha catalytic subunit (PRKAA1 or PRKAA2), a beta (PRKAB1 or PRKAB2) and a gamma non-catalytic subunits (PRKAG1, PRKAG2 or PRKAG3).By similarity

    Protein-protein interaction databases

    IntActiQ6PAM0. 1 interaction.
    MINTiMINT-4086434.
    STRINGi10090.ENSMUSP00000036410.

    Structurei

    3D structure databases

    ProteinModelPortaliQ6PAM0.
    SMRiQ6PAM0. Positions 74-271.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiNOG238368.
    GeneTreeiENSGT00390000001416.
    HOGENOMiHOG000230597.
    HOVERGENiHBG050430.
    InParanoidiQ6PAM0.
    KOiK07199.
    OMAiESKYITV.
    PhylomeDBiQ6PAM0.
    TreeFamiTF313827.

    Family and domain databases

    InterProiIPR006828. AMP_prot_kin_bsu_interact-dom.
    IPR014756. Ig_E-set.
    [Graphical view]
    PfamiPF04739. AMPKBI. 1 hit.
    [Graphical view]
    SMARTiSM01010. AMPKBI. 1 hit.
    [Graphical view]
    SUPFAMiSSF81296. SSF81296. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q6PAM0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGNTTSERVS GERHGAKAAR AEGGGHGPGK EHKIMVGSTD DPSVFSLPDS    50
    KLPGDKEFVP WQQDLDDSVK PAQQARPTVI RWSEGGKEVF ISGSFNNWST 100
    KIPLIKSHND FVAILDLPEG EHQYKFFVDG QWVHDPSEPV VTSQLGTINN 150
    LIHVKKSDFE VFDALKLDSM ESSETSCRDL SSSPPGPYGQ EMYVFRSEER 200
    FKSPPILPPH LLQVILNKDT NISCDPALLP EPNHVMLNHL YALSIKDSVM 250
    VLSATHRYKK KYVTTLLYKP I 271
    Length:271
    Mass (Da):30,209
    Last modified:July 5, 2004 - v1
    Checksum:i883B42716E996BE7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC060228 mRNA. Translation: AAH60228.1.
    CCDSiCCDS17657.1.
    RefSeqiNP_892042.2. NM_182997.2.
    XP_006500903.1. XM_006500840.1.
    UniGeneiMm.31175.

    Genome annotation databases

    EnsembliENSMUST00000045743; ENSMUSP00000036410; ENSMUSG00000038205.
    GeneIDi108097.
    KEGGimmu:108097.
    UCSCiuc008qpb.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC060228 mRNA. Translation: AAH60228.1 .
    CCDSi CCDS17657.1.
    RefSeqi NP_892042.2. NM_182997.2.
    XP_006500903.1. XM_006500840.1.
    UniGenei Mm.31175.

    3D structure databases

    ProteinModelPortali Q6PAM0.
    SMRi Q6PAM0. Positions 74-271.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q6PAM0. 1 interaction.
    MINTi MINT-4086434.
    STRINGi 10090.ENSMUSP00000036410.

    Protein family/group databases

    CAZyi CBM48. Carbohydrate-Binding Module Family 48.

    PTM databases

    PhosphoSitei Q6PAM0.

    Proteomic databases

    MaxQBi Q6PAM0.
    PaxDbi Q6PAM0.
    PRIDEi Q6PAM0.

    Protocols and materials databases

    DNASUi 108097.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000045743 ; ENSMUSP00000036410 ; ENSMUSG00000038205 .
    GeneIDi 108097.
    KEGGi mmu:108097.
    UCSCi uc008qpb.1. mouse.

    Organism-specific databases

    CTDi 5565.
    MGIi MGI:1336185. Prkab2.

    Phylogenomic databases

    eggNOGi NOG238368.
    GeneTreei ENSGT00390000001416.
    HOGENOMi HOG000230597.
    HOVERGENi HBG050430.
    InParanoidi Q6PAM0.
    KOi K07199.
    OMAi ESKYITV.
    PhylomeDBi Q6PAM0.
    TreeFami TF313827.

    Enzyme and pathway databases

    Reactomei REACT_199054. Translocation of GLUT4 to the plasma membrane.
    REACT_205688. Activation of PPARGC1A (PGC-1alpha) by phosphorylation.
    REACT_220701. Regulation of AMPK activity via LKB1.

    Miscellaneous databases

    ChiTaRSi PRKAB2. mouse.
    NextBioi 360054.
    PROi Q6PAM0.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q6PAM0.
    Bgeei Q6PAM0.
    Genevestigatori Q6PAM0.

    Family and domain databases

    InterProi IPR006828. AMP_prot_kin_bsu_interact-dom.
    IPR014756. Ig_E-set.
    [Graphical view ]
    Pfami PF04739. AMPKBI. 1 hit.
    [Graphical view ]
    SMARTi SM01010. AMPKBI. 1 hit.
    [Graphical view ]
    SUPFAMi SSF81296. SSF81296. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Brain.
    2. "Ulk1-mediated phosphorylation of AMPK constitutes a negative regulatory feedback loop."
      Loffler A.S., Alers S., Dieterle A.M., Keppeler H., Franz-Wachtel M., Kundu M., Campbell D.G., Wesselborg S., Alessi D.R., Stork B.
      Autophagy 7:696-706(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION BY ULK1 AND ULK2.

    Entry informationi

    Entry nameiAAKB2_MOUSE
    AccessioniPrimary (citable) accession number: Q6PAM0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 12, 2005
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 80 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3