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Protein

DENN domain-containing protein 5A

Gene

Dennd5a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Guanine nucleotide exchange factor (GEF) which may activate RAB6A and RAB39A and/or RAB39B. Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP-bound form (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Enzyme and pathway databases

ReactomeiR-MMU-8876198. RAB GEFs exchange GTP for GDP on RABs.

Names & Taxonomyi

Protein namesi
Recommended name:
DENN domain-containing protein 5A
Alternative name(s):
Rab6-interacting protein 1
Short name:
Rab6IP1
Gene namesi
Name:Dennd5a
Synonyms:Rab6ip1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1201681. Dennd5a.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: GOC
  • Golgi apparatus Source: UniProtKB
  • Golgi membrane Source: UniProtKB-SubCell
  • membrane Source: GO_Central
  • retromer complex Source: Ensembl
  • trans-Golgi network Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi763K → E: Abolishes the interaction with RAP6A and localization to Golgi membrane. 1 Publication1
Mutagenesisi932Y → S: Abolishes the interaction with RAP6A and localization to Golgi membrane; when associated with Ala-935. 1 Publication1
Mutagenesisi935L → A: Abolishes the interaction with RAP6A and localization to Golgi membrane; when associated with Ser-932. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000971431 – 1287DENN domain-containing protein 5AAdd BLAST1287

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei193PhosphoserineBy similarity1
Modified residuei1079PhosphothreonineCombined sources1
Modified residuei1085PhosphoserineCombined sources1
Modified residuei1087PhosphoserineBy similarity1
Modified residuei1096PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ6PAL8.
PaxDbiQ6PAL8.
PeptideAtlasiQ6PAL8.
PRIDEiQ6PAL8.

PTM databases

iPTMnetiQ6PAL8.
PhosphoSitePlusiQ6PAL8.

Expressioni

Gene expression databases

BgeeiENSMUSG00000035901.
ExpressionAtlasiQ6PAL8. baseline and differential.
GenevisibleiQ6PAL8. MM.

Interactioni

Subunit structurei

Interacts with RAB6A bound to GTP.2 Publications

GO - Molecular functioni

  • Rab GTPase binding Source: MGI

Protein-protein interaction databases

BioGridi202551. 1 interactor.
DIPiDIP-287N.
STRINGi10090.ENSMUSP00000079295.

Structurei

Secondary structure

11287
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi742 – 772Combined sources31
Helixi784 – 805Combined sources22
Beta strandi806 – 808Combined sources3
Beta strandi812 – 814Combined sources3
Helixi816 – 830Combined sources15
Helixi864 – 872Combined sources9
Helixi880 – 894Combined sources15
Helixi897 – 904Combined sources8
Helixi908 – 914Combined sources7
Turni920 – 922Combined sources3
Helixi924 – 934Combined sources11
Helixi935 – 938Combined sources4
Turni944 – 948Combined sources5
Beta strandi952 – 962Combined sources11
Beta strandi975 – 983Combined sources9
Beta strandi997 – 1003Combined sources7
Beta strandi1007 – 1015Combined sources9
Beta strandi1024 – 1033Combined sources10
Turni1034 – 1036Combined sources3
Beta strandi1039 – 1049Combined sources11
Beta strandi1057 – 1062Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3CWZX-ray3.20B707-1090[»]
ProteinModelPortaliQ6PAL8.
SMRiQ6PAL8.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ6PAL8.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini68 – 138UDENNAdd BLAST71
Domaini202 – 390DENNPROSITE-ProRule annotationAdd BLAST189
Domaini512 – 588dDENNPROSITE-ProRule annotationAdd BLAST77
Domaini787 – 950RUN 1PROSITE-ProRule annotationAdd BLAST164
Domaini954 – 1062PLATPROSITE-ProRule annotationAdd BLAST109
Domaini1134 – 1280RUN 2PROSITE-ProRule annotationAdd BLAST147

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi3 – 9Poly-Gly7
Compositional biasi593 – 598Poly-Asp6

Sequence similaritiesi

Belongs to the RAB6IP1 family.Curated
Contains 1 dDENN domain.PROSITE-ProRule annotation
Contains 1 DENN domain.PROSITE-ProRule annotation
Contains 1 PLAT domain.PROSITE-ProRule annotation
Contains 2 RUN domains.PROSITE-ProRule annotation
Contains 1 uDENN domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG2080. Eukaryota.
ENOG410XNYP. LUCA.
GeneTreeiENSGT00760000118819.
HOGENOMiHOG000019389.
HOVERGENiHBG059906.
InParanoidiQ6PAL8.
KOiK20164.
OMAiAKWLVEC.
OrthoDBiEOG091G00VU.
PhylomeDBiQ6PAL8.
TreeFamiTF313237.

Family and domain databases

Gene3Di2.60.60.20. 1 hit.
InterProiIPR005112. dDENN_dom.
IPR001194. DENN_dom.
IPR001024. PLAT/LH2_dom.
IPR004012. Run_dom.
IPR005113. uDENN_dom.
[Graphical view]
PfamiPF03455. dDENN. 1 hit.
PF02141. DENN. 1 hit.
PF01477. PLAT. 1 hit.
PF02759. RUN. 2 hits.
PF03456. uDENN. 1 hit.
[Graphical view]
SMARTiSM00801. dDENN. 1 hit.
SM00799. DENN. 1 hit.
SM00593. RUN. 2 hits.
SM00800. uDENN. 1 hit.
[Graphical view]
SUPFAMiSSF49723. SSF49723. 1 hit.
PROSITEiPS50947. DDENN. 1 hit.
PS50211. DENN. 1 hit.
PS50095. PLAT. 1 hit.
PS50826. RUN. 2 hits.
PS50946. UDENN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6PAL8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGGGGGGGS APSRFADYFV ICGLDTETGL EPDELSALCQ YIQASKARDG
60 70 80 90 100
ASPFISSTTE GENFEQTPLR RTFKSKVLAR YPENVDWNPF DQDAVGMLCM
110 120 130 140 150
PKGLAFKTQA DPREPQFHAF IITREDGSRT FGFALTFYEE VTSKQICSAM
160 170 180 190 200
QTLYHMHNAE YDVLHAPLAD GGDQSGMEDG EGIPGTKLQR FNSYDISRDT
210 220 230 240 250
LYVSKCICLI TPMSFMKACR SVLQQLHQAV TSPQPPPLPL ESYIYNVLYE
260 270 280 290 300
VPLPPPGRSL KFSGVYGPII CQRPSTNELP LFDFPVKEVF ELLGVENVFQ
310 320 330 340 350
LFTCALLEFQ ILLYSQHYQR LMTVAETITA LMFPFQWQHV YVPILPASLL
360 370 380 390 400
HFLDAPVPYL MGLHSNGLDD RSKLELPQEA NLCFVDVDNH FIELPEDLPQ
410 420 430 440 450
FPNKLEFVQE VSEILMAFGV PPEGNLHCSE SASKLKRIRA SELVSDKRNG
460 470 480 490 500
NIAGSPLHSY ELLKENETIA RLQALVKRTG VSLEKLEVRE DPSSNKDFKV
510 520 530 540 550
QCDEEELRIY QLNIQIREVF ANRFTQMFAD YEVFVIQPSQ DKESWFTNRE
560 570 580 590 600
QMQNFDKASF LSDQPEPYLP FLSRFLETQM FASFIDNKIM CHDDDDKDPV
610 620 630 640 650
LRVFDSRVDK IRLLNVRTPT LRTSMYQKCT TVDEAEKAIE LRLAKIDHTA
660 670 680 690 700
VHPHLLDMKI GQGKYEPGFF PKLQSDVLCT GPASNKWTKR NAPAQWRRKD
710 720 730 740 750
RQKQHTEHLR LDNDQREKYI QEARNMGSTI RQPKLSNLSP SVIAQTNWKF
760 770 780 790 800
VEGLLKECRN KTKRMLVEKM GREAVELGHG EVNITGVEEN TLIASLCDLL
810 820 830 840 850
ERIWSHGLQV KQGKSALWSH LLHYQENRQR KLTSGSLSTS GILLDSERRK
860 870 880 890 900
SDASAVMSPL RISLIQDMRH IQNIGEIKTD VGKARAWVRL SMEKKLLSRH
910 920 930 940 950
LKQLLSDHEL TKKLYKRYAF LRCDDEKEQF LYHLLSFNAV DYFCFTNVFT
960 970 980 990 1000
TILIPYHILI VPSKKLGGSM FTANPWICIS GELGETQILQ IPRNVLEMTF
1010 1020 1030 1040 1050
ECQNLGKLTT VQIGHDNSGL YAKWLVECVM VRNEVTGHTY KFPCGRWLGK
1060 1070 1080 1090 1100
GMDDGSLERV LVGELLTSLP EVDERPCRTP PLQQSPSVIR RLVTISPNNK
1110 1120 1130 1140 1150
PKLNTGQIQE SIGEAVNGIV KHFHKPEKER GSLTLLLCGE CGLVSALEQA
1160 1170 1180 1190 1200
FQHGFKSPRL FKNVFIWDFL EKAQTYYETL EQNDVVPEEN WHTRARNFCR
1210 1220 1230 1240 1250
FVTAVNNTPR NIGKDGKFQM LVCLGARDHL LHHWIALLAD CPITAHMYED
1260 1270 1280
VALIKDHTLV NSLIRVLQTL QEFNITLDTS LVKGIDI
Length:1,287
Mass (Da):146,653
Last modified:July 5, 2004 - v1
Checksum:i9A48FE3DDC6A2BF1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti37 – 60Missing in CAB55599 (Ref. 1) CuratedAdd BLAST24
Sequence conflicti706T → E in AAA78787 (PubMed:7782346).Curated1
Sequence conflicti761K → E in BAC33389 (PubMed:16141072).Curated1
Sequence conflicti871 – 872IQ → ME in AAA78787 (PubMed:7782346).Curated2
Sequence conflicti888 – 890VRL → GAT in AAA78787 (PubMed:7782346).Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ245569 mRNA. Translation: CAB55599.1.
BC022119 mRNA. Translation: AAH22119.1.
BC060230 mRNA. Translation: AAH60230.1.
AK048609 mRNA. Translation: BAC33389.1.
L40894 mRNA. Translation: AAA78787.1.
CCDSiCCDS21741.1.
PIRiA56956.
RefSeqiNP_001298068.1. NM_001311139.1.
NP_067469.1. NM_021494.1.
UniGeneiMm.21904.

Genome annotation databases

EnsembliENSMUST00000080437; ENSMUSP00000079295; ENSMUSG00000035901.
GeneIDi19347.
KEGGimmu:19347.
UCSCiuc009jek.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ245569 mRNA. Translation: CAB55599.1.
BC022119 mRNA. Translation: AAH22119.1.
BC060230 mRNA. Translation: AAH60230.1.
AK048609 mRNA. Translation: BAC33389.1.
L40894 mRNA. Translation: AAA78787.1.
CCDSiCCDS21741.1.
PIRiA56956.
RefSeqiNP_001298068.1. NM_001311139.1.
NP_067469.1. NM_021494.1.
UniGeneiMm.21904.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3CWZX-ray3.20B707-1090[»]
ProteinModelPortaliQ6PAL8.
SMRiQ6PAL8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202551. 1 interactor.
DIPiDIP-287N.
STRINGi10090.ENSMUSP00000079295.

PTM databases

iPTMnetiQ6PAL8.
PhosphoSitePlusiQ6PAL8.

Proteomic databases

MaxQBiQ6PAL8.
PaxDbiQ6PAL8.
PeptideAtlasiQ6PAL8.
PRIDEiQ6PAL8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000080437; ENSMUSP00000079295; ENSMUSG00000035901.
GeneIDi19347.
KEGGimmu:19347.
UCSCiuc009jek.1. mouse.

Organism-specific databases

CTDi23258.
MGIiMGI:1201681. Dennd5a.

Phylogenomic databases

eggNOGiKOG2080. Eukaryota.
ENOG410XNYP. LUCA.
GeneTreeiENSGT00760000118819.
HOGENOMiHOG000019389.
HOVERGENiHBG059906.
InParanoidiQ6PAL8.
KOiK20164.
OMAiAKWLVEC.
OrthoDBiEOG091G00VU.
PhylomeDBiQ6PAL8.
TreeFamiTF313237.

Enzyme and pathway databases

ReactomeiR-MMU-8876198. RAB GEFs exchange GTP for GDP on RABs.

Miscellaneous databases

ChiTaRSiDennd5a. mouse.
EvolutionaryTraceiQ6PAL8.
PROiQ6PAL8.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000035901.
ExpressionAtlasiQ6PAL8. baseline and differential.
GenevisibleiQ6PAL8. MM.

Family and domain databases

Gene3Di2.60.60.20. 1 hit.
InterProiIPR005112. dDENN_dom.
IPR001194. DENN_dom.
IPR001024. PLAT/LH2_dom.
IPR004012. Run_dom.
IPR005113. uDENN_dom.
[Graphical view]
PfamiPF03455. dDENN. 1 hit.
PF02141. DENN. 1 hit.
PF01477. PLAT. 1 hit.
PF02759. RUN. 2 hits.
PF03456. uDENN. 1 hit.
[Graphical view]
SMARTiSM00801. dDENN. 1 hit.
SM00799. DENN. 1 hit.
SM00593. RUN. 2 hits.
SM00800. uDENN. 1 hit.
[Graphical view]
SUPFAMiSSF49723. SSF49723. 1 hit.
PROSITEiPS50947. DDENN. 1 hit.
PS50211. DENN. 1 hit.
PS50095. PLAT. 1 hit.
PS50826. RUN. 2 hits.
PS50946. UDENN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDEN5A_MOUSE
AccessioniPrimary (citable) accession number: Q6PAL8
Secondary accession number(s): Q62146
, Q8C829, Q8VDF6, Q9QYZ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2004
Last sequence update: July 5, 2004
Last modified: November 30, 2016
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.