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Protein

DENN domain-containing protein 5A

Gene

Dennd5a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Guanine nucleotide exchange factor (GEF) which may activate RAB6A and RAB39A and/or RAB39B. Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP-bound form (By similarity).By similarity

GO - Molecular functioni

  • Rab GTPase binding Source: MGI
  • Rab guanyl-nucleotide exchange factor activity Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Names & Taxonomyi

Protein namesi
Recommended name:
DENN domain-containing protein 5A
Alternative name(s):
Rab6-interacting protein 1
Short name:
Rab6IP1
Gene namesi
Name:Dennd5a
Synonyms:Rab6ip1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1201681. Dennd5a.

Subcellular locationi

GO - Cellular componenti

  • Golgi apparatus Source: UniProtKB
  • Golgi membrane Source: UniProtKB-SubCell
  • retromer complex Source: Ensembl
  • trans-Golgi network Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi763 – 7631K → E: Abolishes the interaction with RAP6A and localization to Golgi membrane. 1 Publication
Mutagenesisi932 – 9321Y → S: Abolishes the interaction with RAP6A and localization to Golgi membrane; when associated with Ala-935. 1 Publication
Mutagenesisi935 – 9351L → A: Abolishes the interaction with RAP6A and localization to Golgi membrane; when associated with Ser-932. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12871287DENN domain-containing protein 5APRO_0000097143Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei193 – 1931PhosphoserineBy similarity
Modified residuei1079 – 10791PhosphothreonineCombined sources
Modified residuei1085 – 10851PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ6PAL8.
PaxDbiQ6PAL8.
PRIDEiQ6PAL8.

PTM databases

iPTMnetiQ6PAL8.
PhosphoSiteiQ6PAL8.

Expressioni

Gene expression databases

BgeeiQ6PAL8.
GenevisibleiQ6PAL8. MM.

Interactioni

Subunit structurei

Interacts with RAB6A bound to GTP.2 Publications

GO - Molecular functioni

  • Rab GTPase binding Source: MGI

Protein-protein interaction databases

BioGridi202551. 1 interaction.
DIPiDIP-287N.
STRINGi10090.ENSMUSP00000079295.

Structurei

Secondary structure

1
1287
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi742 – 77231Combined sources
Helixi784 – 80522Combined sources
Beta strandi806 – 8083Combined sources
Beta strandi812 – 8143Combined sources
Helixi816 – 83015Combined sources
Helixi864 – 8729Combined sources
Helixi880 – 89415Combined sources
Helixi897 – 9048Combined sources
Helixi908 – 9147Combined sources
Turni920 – 9223Combined sources
Helixi924 – 93411Combined sources
Helixi935 – 9384Combined sources
Turni944 – 9485Combined sources
Beta strandi952 – 96211Combined sources
Beta strandi975 – 9839Combined sources
Beta strandi997 – 10037Combined sources
Beta strandi1007 – 10159Combined sources
Beta strandi1024 – 103310Combined sources
Turni1034 – 10363Combined sources
Beta strandi1039 – 104911Combined sources
Beta strandi1057 – 10626Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3CWZX-ray3.20B707-1090[»]
ProteinModelPortaliQ6PAL8.
SMRiQ6PAL8. Positions 737-1072.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ6PAL8.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini68 – 13871UDENNAdd
BLAST
Domaini202 – 390189DENNPROSITE-ProRule annotationAdd
BLAST
Domaini512 – 58877dDENNPROSITE-ProRule annotationAdd
BLAST
Domaini787 – 950164RUN 1PROSITE-ProRule annotationAdd
BLAST
Domaini954 – 1062109PLATPROSITE-ProRule annotationAdd
BLAST
Domaini1134 – 1280147RUN 2PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi3 – 97Poly-Gly
Compositional biasi593 – 5986Poly-Asp

Sequence similaritiesi

Belongs to the RAB6IP1 family.Curated
Contains 1 dDENN domain.PROSITE-ProRule annotation
Contains 1 DENN domain.PROSITE-ProRule annotation
Contains 1 PLAT domain.PROSITE-ProRule annotation
Contains 2 RUN domains.PROSITE-ProRule annotation
Contains 1 uDENN domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG2080. Eukaryota.
ENOG410XNYP. LUCA.
GeneTreeiENSGT00760000118819.
HOGENOMiHOG000019389.
HOVERGENiHBG059906.
InParanoidiQ6PAL8.
OMAiAKWLVEC.
OrthoDBiEOG7FJGZP.
PhylomeDBiQ6PAL8.
TreeFamiTF313237.

Family and domain databases

Gene3Di2.60.60.20. 1 hit.
InterProiIPR005112. dDENN_dom.
IPR001194. DENN_dom.
IPR001024. PLAT/LH2_dom.
IPR004012. Run_dom.
IPR005113. uDENN_dom.
[Graphical view]
PfamiPF03455. dDENN. 1 hit.
PF02141. DENN. 1 hit.
PF01477. PLAT. 1 hit.
PF02759. RUN. 2 hits.
PF03456. uDENN. 1 hit.
[Graphical view]
SMARTiSM00801. dDENN. 1 hit.
SM00799. DENN. 1 hit.
SM00593. RUN. 2 hits.
SM00800. uDENN. 1 hit.
[Graphical view]
SUPFAMiSSF49723. SSF49723. 1 hit.
PROSITEiPS50947. DDENN. 1 hit.
PS50211. DENN. 1 hit.
PS50095. PLAT. 1 hit.
PS50826. RUN. 2 hits.
PS50946. UDENN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6PAL8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGGGGGGGS APSRFADYFV ICGLDTETGL EPDELSALCQ YIQASKARDG
60 70 80 90 100
ASPFISSTTE GENFEQTPLR RTFKSKVLAR YPENVDWNPF DQDAVGMLCM
110 120 130 140 150
PKGLAFKTQA DPREPQFHAF IITREDGSRT FGFALTFYEE VTSKQICSAM
160 170 180 190 200
QTLYHMHNAE YDVLHAPLAD GGDQSGMEDG EGIPGTKLQR FNSYDISRDT
210 220 230 240 250
LYVSKCICLI TPMSFMKACR SVLQQLHQAV TSPQPPPLPL ESYIYNVLYE
260 270 280 290 300
VPLPPPGRSL KFSGVYGPII CQRPSTNELP LFDFPVKEVF ELLGVENVFQ
310 320 330 340 350
LFTCALLEFQ ILLYSQHYQR LMTVAETITA LMFPFQWQHV YVPILPASLL
360 370 380 390 400
HFLDAPVPYL MGLHSNGLDD RSKLELPQEA NLCFVDVDNH FIELPEDLPQ
410 420 430 440 450
FPNKLEFVQE VSEILMAFGV PPEGNLHCSE SASKLKRIRA SELVSDKRNG
460 470 480 490 500
NIAGSPLHSY ELLKENETIA RLQALVKRTG VSLEKLEVRE DPSSNKDFKV
510 520 530 540 550
QCDEEELRIY QLNIQIREVF ANRFTQMFAD YEVFVIQPSQ DKESWFTNRE
560 570 580 590 600
QMQNFDKASF LSDQPEPYLP FLSRFLETQM FASFIDNKIM CHDDDDKDPV
610 620 630 640 650
LRVFDSRVDK IRLLNVRTPT LRTSMYQKCT TVDEAEKAIE LRLAKIDHTA
660 670 680 690 700
VHPHLLDMKI GQGKYEPGFF PKLQSDVLCT GPASNKWTKR NAPAQWRRKD
710 720 730 740 750
RQKQHTEHLR LDNDQREKYI QEARNMGSTI RQPKLSNLSP SVIAQTNWKF
760 770 780 790 800
VEGLLKECRN KTKRMLVEKM GREAVELGHG EVNITGVEEN TLIASLCDLL
810 820 830 840 850
ERIWSHGLQV KQGKSALWSH LLHYQENRQR KLTSGSLSTS GILLDSERRK
860 870 880 890 900
SDASAVMSPL RISLIQDMRH IQNIGEIKTD VGKARAWVRL SMEKKLLSRH
910 920 930 940 950
LKQLLSDHEL TKKLYKRYAF LRCDDEKEQF LYHLLSFNAV DYFCFTNVFT
960 970 980 990 1000
TILIPYHILI VPSKKLGGSM FTANPWICIS GELGETQILQ IPRNVLEMTF
1010 1020 1030 1040 1050
ECQNLGKLTT VQIGHDNSGL YAKWLVECVM VRNEVTGHTY KFPCGRWLGK
1060 1070 1080 1090 1100
GMDDGSLERV LVGELLTSLP EVDERPCRTP PLQQSPSVIR RLVTISPNNK
1110 1120 1130 1140 1150
PKLNTGQIQE SIGEAVNGIV KHFHKPEKER GSLTLLLCGE CGLVSALEQA
1160 1170 1180 1190 1200
FQHGFKSPRL FKNVFIWDFL EKAQTYYETL EQNDVVPEEN WHTRARNFCR
1210 1220 1230 1240 1250
FVTAVNNTPR NIGKDGKFQM LVCLGARDHL LHHWIALLAD CPITAHMYED
1260 1270 1280
VALIKDHTLV NSLIRVLQTL QEFNITLDTS LVKGIDI
Length:1,287
Mass (Da):146,653
Last modified:July 5, 2004 - v1
Checksum:i9A48FE3DDC6A2BF1
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti37 – 6024Missing in CAB55599 (Ref. 1) CuratedAdd
BLAST
Sequence conflicti706 – 7061T → E in AAA78787 (PubMed:7782346).Curated
Sequence conflicti761 – 7611K → E in BAC33389 (PubMed:16141072).Curated
Sequence conflicti871 – 8722IQ → ME in AAA78787 (PubMed:7782346).Curated
Sequence conflicti888 – 8903VRL → GAT in AAA78787 (PubMed:7782346).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ245569 mRNA. Translation: CAB55599.1.
BC022119 mRNA. Translation: AAH22119.1.
BC060230 mRNA. Translation: AAH60230.1.
AK048609 mRNA. Translation: BAC33389.1.
L40894 mRNA. Translation: AAA78787.1.
CCDSiCCDS21741.1.
PIRiA56956.
RefSeqiNP_001298068.1. NM_001311139.1.
NP_067469.1. NM_021494.1.
UniGeneiMm.21904.

Genome annotation databases

EnsembliENSMUST00000080437; ENSMUSP00000079295; ENSMUSG00000035901.
GeneIDi19347.
KEGGimmu:19347.
UCSCiuc009jek.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ245569 mRNA. Translation: CAB55599.1.
BC022119 mRNA. Translation: AAH22119.1.
BC060230 mRNA. Translation: AAH60230.1.
AK048609 mRNA. Translation: BAC33389.1.
L40894 mRNA. Translation: AAA78787.1.
CCDSiCCDS21741.1.
PIRiA56956.
RefSeqiNP_001298068.1. NM_001311139.1.
NP_067469.1. NM_021494.1.
UniGeneiMm.21904.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3CWZX-ray3.20B707-1090[»]
ProteinModelPortaliQ6PAL8.
SMRiQ6PAL8. Positions 737-1072.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202551. 1 interaction.
DIPiDIP-287N.
STRINGi10090.ENSMUSP00000079295.

PTM databases

iPTMnetiQ6PAL8.
PhosphoSiteiQ6PAL8.

Proteomic databases

MaxQBiQ6PAL8.
PaxDbiQ6PAL8.
PRIDEiQ6PAL8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000080437; ENSMUSP00000079295; ENSMUSG00000035901.
GeneIDi19347.
KEGGimmu:19347.
UCSCiuc009jek.1. mouse.

Organism-specific databases

CTDi23258.
MGIiMGI:1201681. Dennd5a.

Phylogenomic databases

eggNOGiKOG2080. Eukaryota.
ENOG410XNYP. LUCA.
GeneTreeiENSGT00760000118819.
HOGENOMiHOG000019389.
HOVERGENiHBG059906.
InParanoidiQ6PAL8.
OMAiAKWLVEC.
OrthoDBiEOG7FJGZP.
PhylomeDBiQ6PAL8.
TreeFamiTF313237.

Miscellaneous databases

ChiTaRSiDennd5a. mouse.
EvolutionaryTraceiQ6PAL8.
NextBioi296369.
PROiQ6PAL8.
SOURCEiSearch...

Gene expression databases

BgeeiQ6PAL8.
GenevisibleiQ6PAL8. MM.

Family and domain databases

Gene3Di2.60.60.20. 1 hit.
InterProiIPR005112. dDENN_dom.
IPR001194. DENN_dom.
IPR001024. PLAT/LH2_dom.
IPR004012. Run_dom.
IPR005113. uDENN_dom.
[Graphical view]
PfamiPF03455. dDENN. 1 hit.
PF02141. DENN. 1 hit.
PF01477. PLAT. 1 hit.
PF02759. RUN. 2 hits.
PF03456. uDENN. 1 hit.
[Graphical view]
SMARTiSM00801. dDENN. 1 hit.
SM00799. DENN. 1 hit.
SM00593. RUN. 2 hits.
SM00800. uDENN. 1 hit.
[Graphical view]
SUPFAMiSSF49723. SSF49723. 1 hit.
PROSITEiPS50947. DDENN. 1 hit.
PS50211. DENN. 1 hit.
PS50095. PLAT. 1 hit.
PS50826. RUN. 2 hits.
PS50946. UDENN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Goud B.
    Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: ICR.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Brain and Mammary gland.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 703-1287.
    Strain: C57BL/6J.
    Tissue: Head.
  4. "Two-hybrid system screen with the small GTP-binding protein Rab6. Identification of a novel mouse GDP dissociation inhibitor isoform and two other potential partners of Rab6."
    Janoueix-Lerosey I., Jollivet F., Camonis J., Marche P.N., Goud B.
    J. Biol. Chem. 270:14801-14808(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 705-890, INTERACTION WITH RAB6A.
    Strain: BALB/cJ.
    Tissue: Brain.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-1079 AND SER-1085, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Heart, Kidney, Lung, Spleen and Testis.
  6. "Structural basis for recruitment of Rab6-interacting protein 1 to Golgi via a RUN domain."
    Recacha R., Boulet A., Jollivet F., Monier S., Houdusse A., Goud B., Khan A.R.
    Structure 17:21-30(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.2 ANGSTROMS) OF 707-1090 IN COMPLEX WITH RAB6A, SUBCELLULAR LOCATION, MUTAGENESIS OF LYS-763; TYR-932 AND LEU-935.

Entry informationi

Entry nameiDEN5A_MOUSE
AccessioniPrimary (citable) accession number: Q6PAL8
Secondary accession number(s): Q62146
, Q8C829, Q8VDF6, Q9QYZ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2004
Last sequence update: July 5, 2004
Last modified: May 11, 2016
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.