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Protein

DENN domain-containing protein 5A

Gene

Dennd5a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Guanine nucleotide exchange factor (GEF) which may activate RAB6A and RAB39A and/or RAB39B. Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP-bound form (By similarity). Involved in the negative regulation of neurite outgrowth (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGuanine-nucleotide releasing factor

Enzyme and pathway databases

ReactomeiR-MMU-8876198 RAB GEFs exchange GTP for GDP on RABs

Names & Taxonomyi

Protein namesi
Recommended name:
DENN domain-containing protein 5A
Alternative name(s):
Rab6-interacting protein 1
Short name:
Rab6IP1
Gene namesi
Name:Dennd5a
Synonyms:Rab6ip1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1201681 Dennd5a

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi763K → E: Abolishes the interaction with RAP6A and localization to Golgi membrane. 1 Publication1
Mutagenesisi932Y → S: Abolishes the interaction with RAP6A and localization to Golgi membrane; when associated with Ala-935. 1 Publication1
Mutagenesisi935L → A: Abolishes the interaction with RAP6A and localization to Golgi membrane; when associated with Ser-932. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000971431 – 1287DENN domain-containing protein 5AAdd BLAST1287

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei193PhosphoserineBy similarity1
Modified residuei1079PhosphothreonineCombined sources1
Modified residuei1085PhosphoserineCombined sources1
Modified residuei1087PhosphoserineBy similarity1
Modified residuei1096PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ6PAL8
PaxDbiQ6PAL8
PeptideAtlasiQ6PAL8
PRIDEiQ6PAL8

PTM databases

iPTMnetiQ6PAL8
PhosphoSitePlusiQ6PAL8

Expressioni

Gene expression databases

BgeeiENSMUSG00000035901
ExpressionAtlasiQ6PAL8 baseline and differential
GenevisibleiQ6PAL8 MM

Interactioni

Subunit structurei

Interacts with RAB6A bound to GTP.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
RAB6AP20340-14EBI-15750630,EBI-8851226From a different organism.

GO - Molecular functioni

  • Rab GTPase binding Source: MGI
  • Rab guanyl-nucleotide exchange factor activity Source: UniProtKB

Protein-protein interaction databases

BioGridi202551, 1 interactor
DIPiDIP-287N
IntActiQ6PAL8, 1 interactor
STRINGi10090.ENSMUSP00000079295

Structurei

Secondary structure

11287
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi742 – 772Combined sources31
Helixi784 – 805Combined sources22
Beta strandi806 – 808Combined sources3
Beta strandi812 – 814Combined sources3
Helixi816 – 830Combined sources15
Helixi864 – 872Combined sources9
Helixi880 – 894Combined sources15
Helixi897 – 904Combined sources8
Helixi908 – 914Combined sources7
Turni920 – 922Combined sources3
Helixi924 – 934Combined sources11
Helixi935 – 938Combined sources4
Turni944 – 948Combined sources5
Beta strandi952 – 962Combined sources11
Beta strandi975 – 983Combined sources9
Beta strandi997 – 1003Combined sources7
Beta strandi1007 – 1015Combined sources9
Beta strandi1024 – 1033Combined sources10
Turni1034 – 1036Combined sources3
Beta strandi1039 – 1049Combined sources11
Beta strandi1057 – 1062Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3CWZX-ray3.20B707-1090[»]
ProteinModelPortaliQ6PAL8
SMRiQ6PAL8
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ6PAL8

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini57 – 259uDENNPROSITE-ProRule annotationAdd BLAST203
Domaini278 – 414cDENNPROSITE-ProRule annotationAdd BLAST137
Domaini416 – 598dDENNPROSITE-ProRule annotationAdd BLAST183
Domaini787 – 950RUN 1PROSITE-ProRule annotationAdd BLAST164
Domaini954 – 1062PLATPROSITE-ProRule annotationAdd BLAST109
Domaini1134 – 1280RUN 2PROSITE-ProRule annotationAdd BLAST147

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi3 – 9Poly-Gly7
Compositional biasi593 – 598Poly-Asp6

Sequence similaritiesi

Belongs to the RAB6IP1 family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG2080 Eukaryota
ENOG410XNYP LUCA
GeneTreeiENSGT00760000118819
HOGENOMiHOG000019389
HOVERGENiHBG059906
InParanoidiQ6PAL8
KOiK20164
OMAiAWVRLSM
OrthoDBiEOG091G00VU
PhylomeDBiQ6PAL8
TreeFamiTF313237

Family and domain databases

InterProiView protein in InterPro
IPR001194 cDENN_dom
IPR005112 dDENN_dom
IPR001024 PLAT/LH2_dom
IPR036392 PLAT/LH2_dom_sf
IPR004012 Run_dom
IPR037213 Run_dom_sf
IPR037516 Tripartite_DENN
IPR005113 uDENN_dom
PfamiView protein in Pfam
PF03455 dDENN, 1 hit
PF02141 DENN, 1 hit
PF01477 PLAT, 1 hit
PF02759 RUN, 2 hits
PF03456 uDENN, 1 hit
SMARTiView protein in SMART
SM00801 dDENN, 1 hit
SM00799 DENN, 1 hit
SM00593 RUN, 2 hits
SM00800 uDENN, 1 hit
SUPFAMiSSF140741 SSF140741, 3 hits
SSF49723 SSF49723, 1 hit
PROSITEiView protein in PROSITE
PS50211 DENN, 1 hit
PS50095 PLAT, 1 hit
PS50826 RUN, 2 hits

Sequencei

Sequence statusi: Complete.

Q6PAL8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGGGGGGGS APSRFADYFV ICGLDTETGL EPDELSALCQ YIQASKARDG
60 70 80 90 100
ASPFISSTTE GENFEQTPLR RTFKSKVLAR YPENVDWNPF DQDAVGMLCM
110 120 130 140 150
PKGLAFKTQA DPREPQFHAF IITREDGSRT FGFALTFYEE VTSKQICSAM
160 170 180 190 200
QTLYHMHNAE YDVLHAPLAD GGDQSGMEDG EGIPGTKLQR FNSYDISRDT
210 220 230 240 250
LYVSKCICLI TPMSFMKACR SVLQQLHQAV TSPQPPPLPL ESYIYNVLYE
260 270 280 290 300
VPLPPPGRSL KFSGVYGPII CQRPSTNELP LFDFPVKEVF ELLGVENVFQ
310 320 330 340 350
LFTCALLEFQ ILLYSQHYQR LMTVAETITA LMFPFQWQHV YVPILPASLL
360 370 380 390 400
HFLDAPVPYL MGLHSNGLDD RSKLELPQEA NLCFVDVDNH FIELPEDLPQ
410 420 430 440 450
FPNKLEFVQE VSEILMAFGV PPEGNLHCSE SASKLKRIRA SELVSDKRNG
460 470 480 490 500
NIAGSPLHSY ELLKENETIA RLQALVKRTG VSLEKLEVRE DPSSNKDFKV
510 520 530 540 550
QCDEEELRIY QLNIQIREVF ANRFTQMFAD YEVFVIQPSQ DKESWFTNRE
560 570 580 590 600
QMQNFDKASF LSDQPEPYLP FLSRFLETQM FASFIDNKIM CHDDDDKDPV
610 620 630 640 650
LRVFDSRVDK IRLLNVRTPT LRTSMYQKCT TVDEAEKAIE LRLAKIDHTA
660 670 680 690 700
VHPHLLDMKI GQGKYEPGFF PKLQSDVLCT GPASNKWTKR NAPAQWRRKD
710 720 730 740 750
RQKQHTEHLR LDNDQREKYI QEARNMGSTI RQPKLSNLSP SVIAQTNWKF
760 770 780 790 800
VEGLLKECRN KTKRMLVEKM GREAVELGHG EVNITGVEEN TLIASLCDLL
810 820 830 840 850
ERIWSHGLQV KQGKSALWSH LLHYQENRQR KLTSGSLSTS GILLDSERRK
860 870 880 890 900
SDASAVMSPL RISLIQDMRH IQNIGEIKTD VGKARAWVRL SMEKKLLSRH
910 920 930 940 950
LKQLLSDHEL TKKLYKRYAF LRCDDEKEQF LYHLLSFNAV DYFCFTNVFT
960 970 980 990 1000
TILIPYHILI VPSKKLGGSM FTANPWICIS GELGETQILQ IPRNVLEMTF
1010 1020 1030 1040 1050
ECQNLGKLTT VQIGHDNSGL YAKWLVECVM VRNEVTGHTY KFPCGRWLGK
1060 1070 1080 1090 1100
GMDDGSLERV LVGELLTSLP EVDERPCRTP PLQQSPSVIR RLVTISPNNK
1110 1120 1130 1140 1150
PKLNTGQIQE SIGEAVNGIV KHFHKPEKER GSLTLLLCGE CGLVSALEQA
1160 1170 1180 1190 1200
FQHGFKSPRL FKNVFIWDFL EKAQTYYETL EQNDVVPEEN WHTRARNFCR
1210 1220 1230 1240 1250
FVTAVNNTPR NIGKDGKFQM LVCLGARDHL LHHWIALLAD CPITAHMYED
1260 1270 1280
VALIKDHTLV NSLIRVLQTL QEFNITLDTS LVKGIDI
Length:1,287
Mass (Da):146,653
Last modified:July 5, 2004 - v1
Checksum:i9A48FE3DDC6A2BF1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti37 – 60Missing in CAB55599 (Ref. 1) CuratedAdd BLAST24
Sequence conflicti706T → E in AAA78787 (PubMed:7782346).Curated1
Sequence conflicti761K → E in BAC33389 (PubMed:16141072).Curated1
Sequence conflicti871 – 872IQ → ME in AAA78787 (PubMed:7782346).Curated2
Sequence conflicti888 – 890VRL → GAT in AAA78787 (PubMed:7782346).Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ245569 mRNA Translation: CAB55599.1
BC022119 mRNA Translation: AAH22119.1
BC060230 mRNA Translation: AAH60230.1
AK048609 mRNA Translation: BAC33389.1
L40894 mRNA Translation: AAA78787.1
CCDSiCCDS21741.1
PIRiA56956
RefSeqiNP_001298068.1, NM_001311139.1
NP_067469.1, NM_021494.1
UniGeneiMm.21904

Genome annotation databases

EnsembliENSMUST00000080437; ENSMUSP00000079295; ENSMUSG00000035901
GeneIDi19347
KEGGimmu:19347
UCSCiuc009jek.1 mouse

Similar proteinsi

Entry informationi

Entry nameiDEN5A_MOUSE
AccessioniPrimary (citable) accession number: Q6PAL8
Secondary accession number(s): Q62146
, Q8C829, Q8VDF6, Q9QYZ2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 13, 2004
Last sequence update: July 5, 2004
Last modified: March 28, 2018
This is version 115 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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