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Protein

Draxin

Gene

Draxin

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Chemorepulsive axon guidance protein required for the development of spinal cord and forebrain commissures. Acts as a chemorepulsive guidance protein for commissural axons during development. Able to inhibit or repel neurite outgrowth from dorsal spinal cord. Inhibits the stabilization of cytosolic beta-catenin (CTNNB1) via its interaction with LRP6, thereby acting as an antagonist of Wnt signaling pathway.UniRule annotation1 Publication

GO - Biological processi

  • axon guidance Source: UniProtKB
  • commissural neuron differentiation in spinal cord Source: UniProtKB
  • dorsal spinal cord development Source: UniProtKB
  • forebrain development Source: UniProtKB
  • negative regulation of axon extension Source: MGI
  • negative regulation of canonical Wnt signaling pathway Source: UniProtKB
  • negative regulation of neuron apoptotic process Source: MGI
  • Wnt signaling pathway Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Wnt signaling pathway

Names & Taxonomyi

Protein namesi
Recommended name:
DraxinUniRule annotation
Alternative name(s):
Dorsal inhibitory axon guidance proteinUniRule annotation
Dorsal repulsive axon guidance proteinUniRule annotation
Neucrin
Gene namesi
Name:Draxin
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1917683. Draxin.

Subcellular locationi

  • Secreted UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Disruption phenotypei

Mice are viable and fertile. They however show a defasciculation of spinal cord commissural axons and absence of all forebrain commissures.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525UniRule annotationAdd
BLAST
Chaini26 – 343318DraxinPRO_0000273244Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi258 – 2581N-linked (GlcNAc...)UniRule annotation

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ6PAL1.
PaxDbiQ6PAL1.
PRIDEiQ6PAL1.

PTM databases

PhosphoSiteiQ6PAL1.

Expressioni

Tissue specificityi

Predominantly expressed in developing neural tissues. Expressed in many brain regions, including the olfactory bulb, cortex, midbrain, cerebellum and pontine nuclei in postnatal day 0 (P0). Detected in the dorsal spinal cord and commissural axons. In the forebrain commissures, it is expressed in the regions that surround the corpus callosum, hippocampal commissure, and anterior commissure, such as the midline glial cells, indusium griseum glia, and glial wedge.2 Publications

Gene expression databases

BgeeiQ6PAL1.
CleanExiMM_2610109H07RIK.
GenevisibleiQ6PAL1. MM.

Interactioni

Subunit structurei

Interacts with LRP6.UniRule annotation1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ6PAL1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the draxin family.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IKDT. Eukaryota.
ENOG4111MNU. LUCA.
GeneTreeiENSGT00390000013828.
HOGENOMiHOG000112267.
HOVERGENiHBG107824.
InParanoidiQ6PAL1.
OMAiCMEGLRC.
OrthoDBiEOG7R2BK0.
PhylomeDBiQ6PAL1.
TreeFamiTF333255.

Family and domain databases

HAMAPiMF_03060. Draxin.
InterProiIPR029094. Draxin.
[Graphical view]
PfamiPF15550. Draxin. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6PAL1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGCPVLRVP TLFLILLLFP ELHTAGTLAS GSSARNLPET HSHLPSSALW
60 70 80 90 100
VSQASHHGRR GLGKKDRGPG RPSRAQEGAV VTATKQASQM TLGQPPAGLL
110 120 130 140 150
QNKELLLGLT LPYPEKEARS PAWERVKKRG REHKRRRDRL RLHRGRAAIR
160 170 180 190 200
GPSSLMKKVE PSEDRMLEGT MEESSTSLAP TMFFLTMTDG ATPTTEESRI
210 220 230 240 250
LPVTSLRPQT QPRSDGEVMP TLDMALFDWT DYEDLKPEVW PSAKKKEKHW
260 270 280 290 300
SHFTSDGNET SPAEGDPCDH HQDCLPGTCC DLREHLCTPH NRGLNNKCFD
310 320 330 340
DCMCMEGLRC YAKFHRNRRV TRRKGRCVEP ETANGDQGSF INI
Length:343
Mass (Da):38,363
Last modified:March 29, 2005 - v2
Checksum:i50C71A3654664B93
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti82 – 821T → S in BAG80560 (PubMed:16141072).Curated
Sequence conflicti82 – 821T → S in CAQ51921 (PubMed:15489334).Curated
Sequence conflicti122 – 1221A → G in BAG80560 (PubMed:16141072).Curated
Sequence conflicti122 – 1221A → G in CAQ51921 (PubMed:15489334).Curated
Sequence conflicti186 – 1861T → N in BAC39628 (PubMed:19468303).Curated
Sequence conflicti188 – 1881T → A in BAG80560 (PubMed:16141072).Curated
Sequence conflicti188 – 1881T → A in CAQ51921 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB301918 mRNA. Translation: BAG80560.1.
AK086198 mRNA. Translation: BAC39628.1.
AL606929 Genomic DNA. Translation: CAM14900.1.
CU207376 Genomic DNA. Translation: CAQ51921.1.
BC060239 mRNA. Translation: AAH60239.2.
CCDSiCCDS18931.1.
RefSeqiNP_081702.2. NM_027426.3.
UniGeneiMm.275288.

Genome annotation databases

EnsembliENSMUST00000030862; ENSMUSP00000030862; ENSMUSG00000029005.
GeneIDi70433.
KEGGimmu:70433.
UCSCiuc008vua.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB301918 mRNA. Translation: BAG80560.1.
AK086198 mRNA. Translation: BAC39628.1.
AL606929 Genomic DNA. Translation: CAM14900.1.
CU207376 Genomic DNA. Translation: CAQ51921.1.
BC060239 mRNA. Translation: AAH60239.2.
CCDSiCCDS18931.1.
RefSeqiNP_081702.2. NM_027426.3.
UniGeneiMm.275288.

3D structure databases

ProteinModelPortaliQ6PAL1.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

PhosphoSiteiQ6PAL1.

Proteomic databases

MaxQBiQ6PAL1.
PaxDbiQ6PAL1.
PRIDEiQ6PAL1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000030862; ENSMUSP00000030862; ENSMUSG00000029005.
GeneIDi70433.
KEGGimmu:70433.
UCSCiuc008vua.2. mouse.

Organism-specific databases

CTDi374946.
MGIiMGI:1917683. Draxin.

Phylogenomic databases

eggNOGiENOG410IKDT. Eukaryota.
ENOG4111MNU. LUCA.
GeneTreeiENSGT00390000013828.
HOGENOMiHOG000112267.
HOVERGENiHBG107824.
InParanoidiQ6PAL1.
OMAiCMEGLRC.
OrthoDBiEOG7R2BK0.
PhylomeDBiQ6PAL1.
TreeFamiTF333255.

Miscellaneous databases

PROiQ6PAL1.
SOURCEiSearch...

Gene expression databases

BgeeiQ6PAL1.
CleanExiMM_2610109H07RIK.
GenevisibleiQ6PAL1. MM.

Family and domain databases

HAMAPiMF_03060. Draxin.
InterProiIPR029094. Draxin.
[Graphical view]
PfamiPF15550. Draxin. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Neucrin is a novel neural-specific secreted antagonist to canonical Wnt signaling."
    Miyake A., Takahashi Y., Miwa H., Shimada A., Konishi M., Itoh N.
    Biochem. Biophys. Res. Commun. 390:1051-1055(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH LRP6, TISSUE SPECIFICITY.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Head.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Brain.
  5. Cited for: TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiDRAXI_MOUSE
AccessioniPrimary (citable) accession number: Q6PAL1
Secondary accession number(s): A2A7G4, B2KFL9, Q8C3D8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: March 29, 2005
Last modified: June 8, 2016
This is version 70 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.