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Q6PAJ1 (BCR_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Breakpoint cluster region protein

EC=2.7.11.1
Gene names
Name:Bcr
Synonyms:Kiaa3017
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1270 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

GTPase-activating protein for RAC1 and CDC42. Promotes the exchange of RAC or CDC42-bound GDP by GTP, thereby activating them. Displays serine/threonine kinase activity By similarity.

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Subunit structure

Homotetramer. Interacts with PDZK1. Interacts with HCK, FES/FPS, ABL1, PIK3R1 and GRB2 By similarity. May interact with CCPG1. Ref.5

Domain

The region involved in binding to ABL1 SH2-domain is rich in serine residues and needs to be Ser/Thr phosphorylated prior to SH2 binding. This region is essential for the activation of the ABL1 tyrosine kinase By similarity.

The DH domain is involved in interaction with CCPG1.

Post-translational modification

Autophosphorylated. Phosphorylated by FES/FPS on tyrosine residues, leading to down-regulation of the BCR kinase activity. Phosphorylation at Tyr-178 by HCK is important for interaction with GRB2 By similarity.

Sequence similarities

Contains 1 C2 domain.

Contains 1 DH (DBL-homology) domain.

Contains 1 PH domain.

Contains 1 Rho-GAP domain.

Ontologies

Keywords
   DiseaseProto-oncogene
   LigandATP-binding
Nucleotide-binding
   Molecular functionGTPase activation
Guanine-nucleotide releasing factor
Kinase
Serine/threonine-protein kinase
Transferase
   PTMAcetylation
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processactin cytoskeleton organization

Inferred from genetic interaction PubMed 17116687. Source: MGI

brain development

Inferred from genetic interaction PubMed 11684658. Source: MGI

inner ear morphogenesis

Inferred from genetic interaction PubMed 11921339. Source: MGI

intracellular signal transduction

Inferred from electronic annotation. Source: InterPro

negative regulation of cell migration

Inferred from genetic interaction PubMed 17116687. Source: MGI

negative regulation of inflammatory response

Inferred from mutant phenotype PubMed 19703997. Source: MGI

negative regulation of neutrophil degranulation

Inferred from genetic interaction PubMed 19703997. Source: MGI

neuromuscular process controlling balance

Inferred from genetic interaction PubMed 11921339. Source: MGI

platelet-derived growth factor receptor signaling pathway

Inferred from electronic annotation. Source: Ensembl

positive regulation of GTPase activity

Inferred from direct assay PubMed 17116687. Source: GOC

positive regulation of Rac GTPase activity

Inferred from genetic interaction PubMed 19703997. Source: GOC

positive regulation of phagocytosis

Inferred from genetic interaction PubMed 17116687. Source: MGI

protein autophosphorylation

Inferred from electronic annotation. Source: Ensembl

regulation of cell cycle

Inferred from direct assay PubMed 11809706. Source: MGI

response to lipopolysaccharide

Inferred from mutant phenotype PubMed 19703997. Source: MGI

   Cellular_componentcytosol

Inferred from direct assay PubMed 17116687. Source: MGI

plasma membrane

Inferred from direct assay PubMed 17116687. Source: MGI

protein complex

Inferred from sequence orthology PubMed 23382219. Source: MGI

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

GTPase activator activity

Inferred from direct assay PubMed 17116687. Source: MGI

Rac GTPase activator activity

Inferred from genetic interaction PubMed 19703997. Source: MGI

Rho guanyl-nucleotide exchange factor activity

Inferred from electronic annotation. Source: InterPro

protein binding

Inferred from physical interaction Ref.5. Source: MGI

protein serine/threonine kinase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 12701270Breakpoint cluster region protein
PRO_0000273731

Regions

Domain497 – 690194DH
Domain707 – 865159PH
Domain869 – 1001133C2
Domain1053 – 1247195Rho-GAP
Region198 – 387190Binding to ABL SH2-domain By similarity
Compositional bias135 – 1406Poly-Ala
Compositional bias317 – 38468Ser-rich

Amino acid modifications

Modified residue11N-acetylmethionine By similarity
Modified residue1221Phosphoserine By similarity
Modified residue1781Phosphotyrosine; by HCK By similarity
Modified residue2161Phosphoserine By similarity
Modified residue2371Phosphoserine Ref.7
Modified residue2471Phosphotyrosine; by FES Ref.7
Modified residue4611Phosphoserine By similarity
Modified residue12631Phosphoserine By similarity

Experimental info

Sequence conflict4181S → H in CAA37013. Ref.3
Sequence conflict12131P → S in AAH60270. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q6PAJ1 [UniParc].

Last modified July 27, 2011. Version 3.
Checksum: 2F3E6D8485DE6717

FASTA1,270143,072
        10         20         30         40         50         60 
MVDSVGFAEA WRAQFPDSEP PRMELRSVGD IEQELERCKA SIRRLEQEVN QERFRMIYLQ 

        70         80         90        100        110        120 
TLLAKEKKSY DRQRWGFRRA AQPPDGAAEP RASAPRPPPA PADGADPAPV EESEARPDGE 

       130        140        150        160        170        180 
GSPSKGRSAS ARRPAAAASA DRDDRGPPTS VAALRSNFEK IRKGPAQPGS ADAEKPFYVN 

       190        200        210        220        230        240 
VEFHHERGLV KVNDKEVSDR ISSLGSQAMQ MERKKSQQSA GQGLGEAPRP HYRGRSSESS 

       250        260        270        280        290        300 
CGLDGDYEDA ELNPRFLKDN LINANGGNRP PWPPLEYQPY QSIYVGGMMV EGEGKSPLLR 

       310        320        330        340        350        360 
SQSTSEQEKR LTWPRRSYSP RSFEDSGGGY TPDCSSNENL TSSEEDFSSG QSSRVSPSPT 

       370        380        390        400        410        420 
TYRMFRDKSR SPSQNSQQSF DSSSPPTPQC QKRHRQCQVV VSEATIVGVR KTGQIWPSDG 

       430        440        450        460        470        480 
DSTFQGEADS SFGTPPGYGC AADQAEEQRR HQDGLPYIDD SPSSSPHLSS KGRGSLASGA 

       490        500        510        520        530        540 
LDPTKVSELD LEKGLEMRKW VLSGILASEE TYLSHLEALL LPMKPLKAAA TTSQPVLTSQ 

       550        560        570        580        590        600 
QIETIFFKVP ELYEIHKEFY DGLFPRVQQW SHQQRVGDLF QKLASQLGVY RAFVDNYGVA 

       610        620        630        640        650        660 
METAEKCCQA NAQFAEISEN LRARSNKDVK DSTTKNSLET LLYKPVDRVT RSTLVLHDLL 

       670        680        690        700        710        720 
KHTPSSHPDH SLLQDALRIS QNFLSSINEE ITPRRQSMTV KKGEHRQLLK DSFMVELVEG 

       730        740        750        760        770        780 
ARKLRHIFLF TDLLLCTKLK KQSGGKTQQY DCKWYIPLTD LSFQMVDELE ALPNIPLVPD 

       790        800        810        820        830        840 
EELDALKIKI SQIKSDIQRE KRANKGSKVM ERLRKKLSEQ ESLLLLMSPS MAFRVHSRNG 

       850        860        870        880        890        900 
KSYTFLISSD YERAEWRESI REQQKKCFKS FSLTSVELQM LTNSCVKLQT VHHIPLTINK 

       910        920        930        940        950        960 
EDDESPGLYG FLHVIVHSAT GFKQSSNLYC TLEVDSFGYF VNKAKTRVYR DTTEPNWNEE 

       970        980        990       1000       1010       1020 
FEIELEGSQT LRILCYEKCY NKMKMTKEDG ESADKLMGKG QVQLDPQTLQ DRDWQRTVID 

      1030       1040       1050       1060       1070       1080 
MNGIEVKLSV KFTSREFSLK RMPSRKQTGV FGVKIAVVTK RERSKVPYIV RQCVEEIERR 

      1090       1100       1110       1120       1130       1140 
GMEEVGIYRV SGVATDIQAL KAAFDVNNKD VSVMMSEMDV NAIAGTLKLY FRELPEPLFT 

      1150       1160       1170       1180       1190       1200 
DEFYPNFAEG IALSDPVAKE SCMLNLLLSL PEANLLTFLF LLDHLKRVAE KETVNKMSLH 

      1210       1220       1230       1240       1250       1260 
NLATVFGPTL LRPSEKESKL PANPSQPITM TDSWSLEVMS QVQVLLYFLQ LEAIPAPDSK 

      1270 
RQSILFSTEV 

« Hide

References

« Hide 'large scale' references
[1]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 212-1270.
Strain: C57BL/6 and FVB/N.
Tissue: Brain and Mammary gland.
[3]"Unique organization of the human BCR gene promoter."
Zhu Q.S., Heisterkamp N., Groffen J.
Nucleic Acids Res. 18:7119-7125(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 276-428.
[4]"Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H.
DNA Res. 10:167-180(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 561-1270.
Tissue: Embryonic tail.
[5]"Ccpg1, a novel scaffold protein that regulates the activity of the Rho guanine nucleotide exchange factor Dbs."
Kostenko E.V., Olabisi O.O., Sahay S., Rodriguez P.L., Whitehead I.P.
Mol. Cell. Biol. 26:8964-8975(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: POSSIBLE INTERACTION WITH CCPG1.
[6]"Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Brain.
[7]"Quantitative time-resolved phosphoproteomic analysis of mast cell signaling."
Cao L., Yu K., Banh C., Nguyen V., Ritz A., Raphael B.J., Kawakami Y., Kawakami T., Salomon A.R.
J. Immunol. 179:5864-5876(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-237 AND TYR-247, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Mast cell.
[8]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC160402 Genomic DNA. No translation available.
BC002193 mRNA. Translation: AAH02193.1.
BC060270 mRNA. Translation: AAH60270.1.
X52831 Genomic DNA. Translation: CAA37013.1.
AK129482 mRNA. Translation: BAC98292.1.
CCDSCCDS35935.1.
PIRS14193. S14194.
RefSeqNP_001074881.1. NM_001081412.2.
UniGeneMm.333722.

3D structure databases

ProteinModelPortalQ6PAJ1.
SMRQ6PAJ1. Positions 5-66.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid225455. 10 interactions.
IntActQ6PAJ1. 1 interaction.
STRING10090.ENSMUSP00000039247.

PTM databases

PhosphoSiteQ6PAJ1.

Proteomic databases

MaxQBQ6PAJ1.
PaxDbQ6PAJ1.
PRIDEQ6PAJ1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000164107; ENSMUSP00000126377; ENSMUSG00000009681.
GeneID110279.
KEGGmmu:110279.
UCSCuc007fqb.2. mouse.

Organism-specific databases

CTD613.
MGIMGI:88141. Bcr.
RougeSearch...

Phylogenomic databases

eggNOGNOG267747.
GeneTreeENSGT00730000110473.
HOGENOMHOG000006779.
HOVERGENHBG004165.
InParanoidQ6PAJ1.
KOK08878.
OMAKRANSHS.
OrthoDBEOG7BP81P.
TreeFamTF105082.

Gene expression databases

ArrayExpressQ6PAJ1.
BgeeQ6PAJ1.
CleanExMM_BCR.
GenevestigatorQ6PAJ1.

Family and domain databases

Gene3D1.10.555.10. 1 hit.
1.20.900.10. 1 hit.
2.30.29.30. 2 hits.
2.60.40.150. 1 hit.
4.10.280.30. 1 hit.
InterProIPR015123. Bcr-Abl_oncoprot_oligo.
IPR000008. C2_dom.
IPR000219. DH-domain.
IPR001331. GDS_CDC24_CS.
IPR011993. PH_like_dom.
IPR001849. Pleckstrin_homology.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
[Graphical view]
PfamPF09036. Bcr-Abl_Oligo. 1 hit.
PF00168. C2. 1 hit.
PF00169. PH. 1 hit.
PF00620. RhoGAP. 1 hit.
PF00621. RhoGEF. 1 hit.
[Graphical view]
SMARTSM00239. C2. 1 hit.
SM00233. PH. 1 hit.
SM00324. RhoGAP. 1 hit.
SM00325. RhoGEF. 1 hit.
[Graphical view]
SUPFAMSSF48065. SSF48065. 1 hit.
SSF48350. SSF48350. 1 hit.
SSF49562. SSF49562. 1 hit.
SSF69036. SSF69036. 1 hit.
PROSITEPS50004. C2. 1 hit.
PS00741. DH_1. 1 hit.
PS50010. DH_2. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS50238. RHOGAP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSBCR. mouse.
NextBio363683.
PROQ6PAJ1.
SOURCESearch...

Entry information

Entry nameBCR_MOUSE
AccessionPrimary (citable) accession number: Q6PAJ1
Secondary accession number(s): E9PZL3 expand/collapse secondary AC list , Q61339, Q6ZPE5, Q99LW5
Entry history
Integrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 92 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot