Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q6PAJ1

- BCR_MOUSE

UniProt

Q6PAJ1 - BCR_MOUSE

Protein

Breakpoint cluster region protein

Gene

Bcr

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 3 (27 Jul 2011)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    GTPase-activating protein for RAC1 and CDC42. Promotes the exchange of RAC or CDC42-bound GDP by GTP, thereby activating them. Displays serine/threonine kinase activity By similarity.By similarity

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. GTPase activator activity Source: MGI
    3. protein binding Source: MGI
    4. protein serine/threonine kinase activity Source: UniProtKB-KW
    5. Rac GTPase activator activity Source: MGI
    6. Rho guanyl-nucleotide exchange factor activity Source: InterPro

    GO - Biological processi

    1. actin cytoskeleton organization Source: MGI
    2. brain development Source: MGI
    3. inner ear morphogenesis Source: MGI
    4. intracellular signal transduction Source: InterPro
    5. negative regulation of cell migration Source: MGI
    6. negative regulation of inflammatory response Source: MGI
    7. negative regulation of neutrophil degranulation Source: MGI
    8. neuromuscular process controlling balance Source: MGI
    9. platelet-derived growth factor receptor signaling pathway Source: Ensembl
    10. positive regulation of GTPase activity Source: GOC
    11. positive regulation of phagocytosis Source: MGI
    12. positive regulation of Rac GTPase activity Source: GOC
    13. protein autophosphorylation Source: Ensembl
    14. regulation of cell cycle Source: MGI
    15. response to lipopolysaccharide Source: MGI

    Keywords - Molecular functioni

    GTPase activation, Guanine-nucleotide releasing factor, Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_196460. Signaling by FGFR1 fusion mutants.
    REACT_210090. Rho GTPase cycle.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Breakpoint cluster region protein (EC:2.7.11.1)
    Gene namesi
    Name:Bcr
    Synonyms:Kiaa3017
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 10

    Organism-specific databases

    MGIiMGI:88141. Bcr.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: MGI
    2. plasma membrane Source: MGI
    3. protein complex Source: MGI

    Pathology & Biotechi

    Keywords - Diseasei

    Proto-oncogene

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 12701270Breakpoint cluster region proteinPRO_0000273731Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity
    Modified residuei122 – 1221PhosphoserineBy similarity
    Modified residuei178 – 1781Phosphotyrosine; by HCKBy similarity
    Modified residuei216 – 2161PhosphoserineBy similarity
    Modified residuei237 – 2371Phosphoserine1 Publication
    Modified residuei247 – 2471Phosphotyrosine; by FES1 Publication
    Modified residuei461 – 4611PhosphoserineBy similarity
    Modified residuei1263 – 12631PhosphoserineBy similarity

    Post-translational modificationi

    Autophosphorylated. Phosphorylated by FES/FPS on tyrosine residues, leading to down-regulation of the BCR kinase activity. Phosphorylation at Tyr-178 by HCK is important for interaction with GRB2 By similarity.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiQ6PAJ1.
    PaxDbiQ6PAJ1.
    PRIDEiQ6PAJ1.

    PTM databases

    PhosphoSiteiQ6PAJ1.

    Expressioni

    Gene expression databases

    ArrayExpressiQ6PAJ1.
    BgeeiQ6PAJ1.
    CleanExiMM_BCR.
    GenevestigatoriQ6PAJ1.

    Interactioni

    Subunit structurei

    Homotetramer. Interacts with PDZK1. Interacts with HCK, FES/FPS, ABL1, PIK3R1 and GRB2 By similarity. May interact with CCPG1.By similarity

    Protein-protein interaction databases

    BioGridi225455. 10 interactions.
    IntActiQ6PAJ1. 1 interaction.
    STRINGi10090.ENSMUSP00000039247.

    Structurei

    3D structure databases

    ProteinModelPortaliQ6PAJ1.
    SMRiQ6PAJ1. Positions 5-66.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini497 – 690194DHPROSITE-ProRule annotationAdd
    BLAST
    Domaini707 – 865159PHPROSITE-ProRule annotationAdd
    BLAST
    Domaini869 – 1001133C2PROSITE-ProRule annotationAdd
    BLAST
    Domaini1053 – 1247195Rho-GAPPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni198 – 387190Binding to ABL SH2-domainBy similarityAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi135 – 1406Poly-Ala
    Compositional biasi317 – 38468Ser-richAdd
    BLAST

    Domaini

    The region involved in binding to ABL1 SH2-domain is rich in serine residues and needs to be Ser/Thr phosphorylated prior to SH2 binding. This region is essential for the activation of the ABL1 tyrosine kinase By similarity.By similarity
    The DH domain is involved in interaction with CCPG1.

    Sequence similaritiesi

    Contains 1 C2 domain.PROSITE-ProRule annotation
    Contains 1 DH (DBL-homology) domain.PROSITE-ProRule annotation
    Contains 1 PH domain.PROSITE-ProRule annotation
    Contains 1 Rho-GAP domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG267747.
    GeneTreeiENSGT00730000110473.
    HOGENOMiHOG000006779.
    HOVERGENiHBG004165.
    InParanoidiQ6PAJ1.
    KOiK08878.
    OMAiKRANSHS.
    OrthoDBiEOG7BP81P.
    TreeFamiTF105082.

    Family and domain databases

    Gene3Di1.10.555.10. 1 hit.
    1.20.900.10. 1 hit.
    2.30.29.30. 2 hits.
    2.60.40.150. 1 hit.
    4.10.280.30. 1 hit.
    InterProiIPR015123. Bcr-Abl_oncoprot_oligo.
    IPR000008. C2_dom.
    IPR000219. DH-domain.
    IPR001331. GDS_CDC24_CS.
    IPR001849. PH_domain.
    IPR011993. PH_like_dom.
    IPR008936. Rho_GTPase_activation_prot.
    IPR000198. RhoGAP_dom.
    [Graphical view]
    PfamiPF09036. Bcr-Abl_Oligo. 1 hit.
    PF00168. C2. 1 hit.
    PF00169. PH. 1 hit.
    PF00620. RhoGAP. 1 hit.
    PF00621. RhoGEF. 1 hit.
    [Graphical view]
    SMARTiSM00239. C2. 1 hit.
    SM00233. PH. 1 hit.
    SM00324. RhoGAP. 1 hit.
    SM00325. RhoGEF. 1 hit.
    [Graphical view]
    SUPFAMiSSF48065. SSF48065. 1 hit.
    SSF48350. SSF48350. 1 hit.
    SSF49562. SSF49562. 1 hit.
    SSF69036. SSF69036. 1 hit.
    PROSITEiPS50004. C2. 1 hit.
    PS00741. DH_1. 1 hit.
    PS50010. DH_2. 1 hit.
    PS50003. PH_DOMAIN. 1 hit.
    PS50238. RHOGAP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q6PAJ1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVDSVGFAEA WRAQFPDSEP PRMELRSVGD IEQELERCKA SIRRLEQEVN     50
    QERFRMIYLQ TLLAKEKKSY DRQRWGFRRA AQPPDGAAEP RASAPRPPPA 100
    PADGADPAPV EESEARPDGE GSPSKGRSAS ARRPAAAASA DRDDRGPPTS 150
    VAALRSNFEK IRKGPAQPGS ADAEKPFYVN VEFHHERGLV KVNDKEVSDR 200
    ISSLGSQAMQ MERKKSQQSA GQGLGEAPRP HYRGRSSESS CGLDGDYEDA 250
    ELNPRFLKDN LINANGGNRP PWPPLEYQPY QSIYVGGMMV EGEGKSPLLR 300
    SQSTSEQEKR LTWPRRSYSP RSFEDSGGGY TPDCSSNENL TSSEEDFSSG 350
    QSSRVSPSPT TYRMFRDKSR SPSQNSQQSF DSSSPPTPQC QKRHRQCQVV 400
    VSEATIVGVR KTGQIWPSDG DSTFQGEADS SFGTPPGYGC AADQAEEQRR 450
    HQDGLPYIDD SPSSSPHLSS KGRGSLASGA LDPTKVSELD LEKGLEMRKW 500
    VLSGILASEE TYLSHLEALL LPMKPLKAAA TTSQPVLTSQ QIETIFFKVP 550
    ELYEIHKEFY DGLFPRVQQW SHQQRVGDLF QKLASQLGVY RAFVDNYGVA 600
    METAEKCCQA NAQFAEISEN LRARSNKDVK DSTTKNSLET LLYKPVDRVT 650
    RSTLVLHDLL KHTPSSHPDH SLLQDALRIS QNFLSSINEE ITPRRQSMTV 700
    KKGEHRQLLK DSFMVELVEG ARKLRHIFLF TDLLLCTKLK KQSGGKTQQY 750
    DCKWYIPLTD LSFQMVDELE ALPNIPLVPD EELDALKIKI SQIKSDIQRE 800
    KRANKGSKVM ERLRKKLSEQ ESLLLLMSPS MAFRVHSRNG KSYTFLISSD 850
    YERAEWRESI REQQKKCFKS FSLTSVELQM LTNSCVKLQT VHHIPLTINK 900
    EDDESPGLYG FLHVIVHSAT GFKQSSNLYC TLEVDSFGYF VNKAKTRVYR 950
    DTTEPNWNEE FEIELEGSQT LRILCYEKCY NKMKMTKEDG ESADKLMGKG 1000
    QVQLDPQTLQ DRDWQRTVID MNGIEVKLSV KFTSREFSLK RMPSRKQTGV 1050
    FGVKIAVVTK RERSKVPYIV RQCVEEIERR GMEEVGIYRV SGVATDIQAL 1100
    KAAFDVNNKD VSVMMSEMDV NAIAGTLKLY FRELPEPLFT DEFYPNFAEG 1150
    IALSDPVAKE SCMLNLLLSL PEANLLTFLF LLDHLKRVAE KETVNKMSLH 1200
    NLATVFGPTL LRPSEKESKL PANPSQPITM TDSWSLEVMS QVQVLLYFLQ 1250
    LEAIPAPDSK RQSILFSTEV 1270
    Length:1,270
    Mass (Da):143,072
    Last modified:July 27, 2011 - v3
    Checksum:i2F3E6D8485DE6717
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti418 – 4181S → H in CAA37013. (PubMed:2263470)Curated
    Sequence conflicti1213 – 12131P → S in AAH60270. (PubMed:15489334)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC160402 Genomic DNA. No translation available.
    BC002193 mRNA. Translation: AAH02193.1.
    BC060270 mRNA. Translation: AAH60270.1.
    X52831 Genomic DNA. Translation: CAA37013.1.
    AK129482 mRNA. Translation: BAC98292.1.
    CCDSiCCDS35935.1.
    PIRiS14194. S14193.
    RefSeqiNP_001074881.1. NM_001081412.2.
    UniGeneiMm.333722.

    Genome annotation databases

    EnsembliENSMUST00000164107; ENSMUSP00000126377; ENSMUSG00000009681.
    GeneIDi110279.
    KEGGimmu:110279.
    UCSCiuc007fqb.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC160402 Genomic DNA. No translation available.
    BC002193 mRNA. Translation: AAH02193.1 .
    BC060270 mRNA. Translation: AAH60270.1 .
    X52831 Genomic DNA. Translation: CAA37013.1 .
    AK129482 mRNA. Translation: BAC98292.1 .
    CCDSi CCDS35935.1.
    PIRi S14194. S14193.
    RefSeqi NP_001074881.1. NM_001081412.2.
    UniGenei Mm.333722.

    3D structure databases

    ProteinModelPortali Q6PAJ1.
    SMRi Q6PAJ1. Positions 5-66.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 225455. 10 interactions.
    IntActi Q6PAJ1. 1 interaction.
    STRINGi 10090.ENSMUSP00000039247.

    PTM databases

    PhosphoSitei Q6PAJ1.

    Proteomic databases

    MaxQBi Q6PAJ1.
    PaxDbi Q6PAJ1.
    PRIDEi Q6PAJ1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000164107 ; ENSMUSP00000126377 ; ENSMUSG00000009681 .
    GeneIDi 110279.
    KEGGi mmu:110279.
    UCSCi uc007fqb.2. mouse.

    Organism-specific databases

    CTDi 613.
    MGIi MGI:88141. Bcr.
    Rougei Search...

    Phylogenomic databases

    eggNOGi NOG267747.
    GeneTreei ENSGT00730000110473.
    HOGENOMi HOG000006779.
    HOVERGENi HBG004165.
    InParanoidi Q6PAJ1.
    KOi K08878.
    OMAi KRANSHS.
    OrthoDBi EOG7BP81P.
    TreeFami TF105082.

    Enzyme and pathway databases

    Reactomei REACT_196460. Signaling by FGFR1 fusion mutants.
    REACT_210090. Rho GTPase cycle.

    Miscellaneous databases

    ChiTaRSi BCR. mouse.
    NextBioi 363683.
    PROi Q6PAJ1.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q6PAJ1.
    Bgeei Q6PAJ1.
    CleanExi MM_BCR.
    Genevestigatori Q6PAJ1.

    Family and domain databases

    Gene3Di 1.10.555.10. 1 hit.
    1.20.900.10. 1 hit.
    2.30.29.30. 2 hits.
    2.60.40.150. 1 hit.
    4.10.280.30. 1 hit.
    InterProi IPR015123. Bcr-Abl_oncoprot_oligo.
    IPR000008. C2_dom.
    IPR000219. DH-domain.
    IPR001331. GDS_CDC24_CS.
    IPR001849. PH_domain.
    IPR011993. PH_like_dom.
    IPR008936. Rho_GTPase_activation_prot.
    IPR000198. RhoGAP_dom.
    [Graphical view ]
    Pfami PF09036. Bcr-Abl_Oligo. 1 hit.
    PF00168. C2. 1 hit.
    PF00169. PH. 1 hit.
    PF00620. RhoGAP. 1 hit.
    PF00621. RhoGEF. 1 hit.
    [Graphical view ]
    SMARTi SM00239. C2. 1 hit.
    SM00233. PH. 1 hit.
    SM00324. RhoGAP. 1 hit.
    SM00325. RhoGEF. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48065. SSF48065. 1 hit.
    SSF48350. SSF48350. 1 hit.
    SSF49562. SSF49562. 1 hit.
    SSF69036. SSF69036. 1 hit.
    PROSITEi PS50004. C2. 1 hit.
    PS00741. DH_1. 1 hit.
    PS50010. DH_2. 1 hit.
    PS50003. PH_DOMAIN. 1 hit.
    PS50238. RHOGAP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 212-1270.
      Strain: C57BL/6 and FVB/N.
      Tissue: Brain and Mammary gland.
    3. "Unique organization of the human BCR gene promoter."
      Zhu Q.S., Heisterkamp N., Groffen J.
      Nucleic Acids Res. 18:7119-7125(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 276-428.
    4. "Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
      Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H.
      DNA Res. 10:167-180(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 561-1270.
      Tissue: Embryonic tail.
    5. "Ccpg1, a novel scaffold protein that regulates the activity of the Rho guanine nucleotide exchange factor Dbs."
      Kostenko E.V., Olabisi O.O., Sahay S., Rodriguez P.L., Whitehead I.P.
      Mol. Cell. Biol. 26:8964-8975(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: POSSIBLE INTERACTION WITH CCPG1.
    6. "Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
      Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
      Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Brain.
    7. "Quantitative time-resolved phosphoproteomic analysis of mast cell signaling."
      Cao L., Yu K., Banh C., Nguyen V., Ritz A., Raphael B.J., Kawakami Y., Kawakami T., Salomon A.R.
      J. Immunol. 179:5864-5876(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-237 AND TYR-247, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Mast cell.
    8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.

    Entry informationi

    Entry nameiBCR_MOUSE
    AccessioniPrimary (citable) accession number: Q6PAJ1
    Secondary accession number(s): E9PZL3
    , Q61339, Q6ZPE5, Q99LW5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 23, 2007
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 94 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3