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Q6PAJ1

- BCR_MOUSE

UniProt

Q6PAJ1 - BCR_MOUSE

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Protein

Breakpoint cluster region protein

Gene

Bcr

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

GTPase-activating protein for RAC1 and CDC42. Promotes the exchange of RAC or CDC42-bound GDP by GTP, thereby activating them. Displays serine/threonine kinase activity By similarity.By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. GTPase activator activity Source: MGI
  3. protein serine/threonine kinase activity Source: UniProtKB-KW
  4. Rac GTPase activator activity Source: MGI
  5. Rho guanyl-nucleotide exchange factor activity Source: InterPro

GO - Biological processi

  1. actin cytoskeleton organization Source: MGI
  2. brain development Source: MGI
  3. inner ear morphogenesis Source: MGI
  4. intracellular signal transduction Source: InterPro
  5. negative regulation of cell migration Source: MGI
  6. negative regulation of inflammatory response Source: MGI
  7. negative regulation of neutrophil degranulation Source: MGI
  8. neuromuscular process controlling balance Source: MGI
  9. platelet-derived growth factor receptor signaling pathway Source: Ensembl
  10. positive regulation of GTPase activity Source: GOC
  11. positive regulation of phagocytosis Source: MGI
  12. positive regulation of Rac GTPase activity Source: GOC
  13. protein autophosphorylation Source: Ensembl
  14. regulation of cell cycle Source: MGI
  15. response to lipopolysaccharide Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

GTPase activation, Guanine-nucleotide releasing factor, Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_196460. Signaling by FGFR1 fusion mutants.
REACT_210090. Rho GTPase cycle.

Names & Taxonomyi

Protein namesi
Recommended name:
Breakpoint cluster region protein (EC:2.7.11.1)
Gene namesi
Name:Bcr
Synonyms:Kiaa3017
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 10

Organism-specific databases

MGIiMGI:88141. Bcr.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: MGI
  2. extracellular vesicular exosome Source: Ensembl
  3. plasma membrane Source: MGI
  4. protein complex Source: MGI
Complete GO annotation...

Pathology & Biotechi

Keywords - Diseasei

Proto-oncogene

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12701270Breakpoint cluster region proteinPRO_0000273731Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei122 – 1221PhosphoserineBy similarity
Modified residuei178 – 1781Phosphotyrosine; by HCKBy similarity
Modified residuei216 – 2161PhosphoserineBy similarity
Modified residuei237 – 2371Phosphoserine1 Publication
Modified residuei247 – 2471Phosphotyrosine; by FES1 Publication
Modified residuei461 – 4611PhosphoserineBy similarity
Modified residuei1263 – 12631PhosphoserineBy similarity

Post-translational modificationi

Autophosphorylated. Phosphorylated by FES/FPS on tyrosine residues, leading to down-regulation of the BCR kinase activity. Phosphorylation at Tyr-178 by HCK is important for interaction with GRB2 By similarity.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ6PAJ1.
PaxDbiQ6PAJ1.
PRIDEiQ6PAJ1.

PTM databases

PhosphoSiteiQ6PAJ1.

Expressioni

Gene expression databases

BgeeiQ6PAJ1.
CleanExiMM_BCR.
ExpressionAtlasiQ6PAJ1. baseline and differential.
GenevestigatoriQ6PAJ1.

Interactioni

Subunit structurei

Homotetramer. Interacts with PDZK1. Interacts with HCK, FES/FPS, ABL1, PIK3R1 and GRB2 By similarity. May interact with CCPG1.By similarity

Protein-protein interaction databases

BioGridi225455. 10 interactions.
IntActiQ6PAJ1. 1 interaction.
STRINGi10090.ENSMUSP00000039247.

Structurei

3D structure databases

ProteinModelPortaliQ6PAJ1.
SMRiQ6PAJ1. Positions 5-66.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini497 – 690194DHPROSITE-ProRule annotationAdd
BLAST
Domaini707 – 865159PHPROSITE-ProRule annotationAdd
BLAST
Domaini869 – 1001133C2PROSITE-ProRule annotationAdd
BLAST
Domaini1053 – 1247195Rho-GAPPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni198 – 387190Binding to ABL SH2-domainBy similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi135 – 1406Poly-Ala
Compositional biasi317 – 38468Ser-richAdd
BLAST

Domaini

The region involved in binding to ABL1 SH2-domain is rich in serine residues and needs to be Ser/Thr phosphorylated prior to SH2 binding. This region is essential for the activation of the ABL1 tyrosine kinase By similarity.By similarity
The DH domain is involved in interaction with CCPG1.

Sequence similaritiesi

Contains 1 C2 domain.PROSITE-ProRule annotation
Contains 1 DH (DBL-homology) domain.PROSITE-ProRule annotation
Contains 1 PH domain.PROSITE-ProRule annotation
Contains 1 Rho-GAP domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG267747.
GeneTreeiENSGT00760000119098.
HOGENOMiHOG000006779.
HOVERGENiHBG004165.
InParanoidiQ6PAJ1.
KOiK08878.
OMAiKRANSHS.
OrthoDBiEOG7BP81P.
TreeFamiTF105082.

Family and domain databases

Gene3Di1.10.555.10. 1 hit.
1.20.900.10. 1 hit.
2.30.29.30. 2 hits.
2.60.40.150. 1 hit.
4.10.280.30. 1 hit.
InterProiIPR015123. Bcr-Abl_oncoprot_oligo.
IPR000008. C2_dom.
IPR000219. DH-domain.
IPR001331. GDS_CDC24_CS.
IPR001849. PH_domain.
IPR011993. PH_like_dom.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
[Graphical view]
PfamiPF09036. Bcr-Abl_Oligo. 1 hit.
PF00168. C2. 1 hit.
PF00169. PH. 1 hit.
PF00620. RhoGAP. 1 hit.
PF00621. RhoGEF. 1 hit.
[Graphical view]
SMARTiSM00239. C2. 1 hit.
SM00233. PH. 1 hit.
SM00324. RhoGAP. 1 hit.
SM00325. RhoGEF. 1 hit.
[Graphical view]
SUPFAMiSSF48065. SSF48065. 1 hit.
SSF48350. SSF48350. 1 hit.
SSF49562. SSF49562. 1 hit.
SSF69036. SSF69036. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS00741. DH_1. 1 hit.
PS50010. DH_2. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS50238. RHOGAP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6PAJ1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVDSVGFAEA WRAQFPDSEP PRMELRSVGD IEQELERCKA SIRRLEQEVN
60 70 80 90 100
QERFRMIYLQ TLLAKEKKSY DRQRWGFRRA AQPPDGAAEP RASAPRPPPA
110 120 130 140 150
PADGADPAPV EESEARPDGE GSPSKGRSAS ARRPAAAASA DRDDRGPPTS
160 170 180 190 200
VAALRSNFEK IRKGPAQPGS ADAEKPFYVN VEFHHERGLV KVNDKEVSDR
210 220 230 240 250
ISSLGSQAMQ MERKKSQQSA GQGLGEAPRP HYRGRSSESS CGLDGDYEDA
260 270 280 290 300
ELNPRFLKDN LINANGGNRP PWPPLEYQPY QSIYVGGMMV EGEGKSPLLR
310 320 330 340 350
SQSTSEQEKR LTWPRRSYSP RSFEDSGGGY TPDCSSNENL TSSEEDFSSG
360 370 380 390 400
QSSRVSPSPT TYRMFRDKSR SPSQNSQQSF DSSSPPTPQC QKRHRQCQVV
410 420 430 440 450
VSEATIVGVR KTGQIWPSDG DSTFQGEADS SFGTPPGYGC AADQAEEQRR
460 470 480 490 500
HQDGLPYIDD SPSSSPHLSS KGRGSLASGA LDPTKVSELD LEKGLEMRKW
510 520 530 540 550
VLSGILASEE TYLSHLEALL LPMKPLKAAA TTSQPVLTSQ QIETIFFKVP
560 570 580 590 600
ELYEIHKEFY DGLFPRVQQW SHQQRVGDLF QKLASQLGVY RAFVDNYGVA
610 620 630 640 650
METAEKCCQA NAQFAEISEN LRARSNKDVK DSTTKNSLET LLYKPVDRVT
660 670 680 690 700
RSTLVLHDLL KHTPSSHPDH SLLQDALRIS QNFLSSINEE ITPRRQSMTV
710 720 730 740 750
KKGEHRQLLK DSFMVELVEG ARKLRHIFLF TDLLLCTKLK KQSGGKTQQY
760 770 780 790 800
DCKWYIPLTD LSFQMVDELE ALPNIPLVPD EELDALKIKI SQIKSDIQRE
810 820 830 840 850
KRANKGSKVM ERLRKKLSEQ ESLLLLMSPS MAFRVHSRNG KSYTFLISSD
860 870 880 890 900
YERAEWRESI REQQKKCFKS FSLTSVELQM LTNSCVKLQT VHHIPLTINK
910 920 930 940 950
EDDESPGLYG FLHVIVHSAT GFKQSSNLYC TLEVDSFGYF VNKAKTRVYR
960 970 980 990 1000
DTTEPNWNEE FEIELEGSQT LRILCYEKCY NKMKMTKEDG ESADKLMGKG
1010 1020 1030 1040 1050
QVQLDPQTLQ DRDWQRTVID MNGIEVKLSV KFTSREFSLK RMPSRKQTGV
1060 1070 1080 1090 1100
FGVKIAVVTK RERSKVPYIV RQCVEEIERR GMEEVGIYRV SGVATDIQAL
1110 1120 1130 1140 1150
KAAFDVNNKD VSVMMSEMDV NAIAGTLKLY FRELPEPLFT DEFYPNFAEG
1160 1170 1180 1190 1200
IALSDPVAKE SCMLNLLLSL PEANLLTFLF LLDHLKRVAE KETVNKMSLH
1210 1220 1230 1240 1250
NLATVFGPTL LRPSEKESKL PANPSQPITM TDSWSLEVMS QVQVLLYFLQ
1260 1270
LEAIPAPDSK RQSILFSTEV
Length:1,270
Mass (Da):143,072
Last modified:July 27, 2011 - v3
Checksum:i2F3E6D8485DE6717
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti418 – 4181S → H in CAA37013. (PubMed:2263470)Curated
Sequence conflicti1213 – 12131P → S in AAH60270. (PubMed:15489334)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC160402 Genomic DNA. No translation available.
BC002193 mRNA. Translation: AAH02193.1.
BC060270 mRNA. Translation: AAH60270.1.
X52831 Genomic DNA. Translation: CAA37013.1.
AK129482 mRNA. Translation: BAC98292.1.
CCDSiCCDS35935.1.
PIRiS14194. S14193.
RefSeqiNP_001074881.1. NM_001081412.2.
UniGeneiMm.333722.

Genome annotation databases

EnsembliENSMUST00000164107; ENSMUSP00000126377; ENSMUSG00000009681.
GeneIDi110279.
KEGGimmu:110279.
UCSCiuc007fqb.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC160402 Genomic DNA. No translation available.
BC002193 mRNA. Translation: AAH02193.1 .
BC060270 mRNA. Translation: AAH60270.1 .
X52831 Genomic DNA. Translation: CAA37013.1 .
AK129482 mRNA. Translation: BAC98292.1 .
CCDSi CCDS35935.1.
PIRi S14194. S14193.
RefSeqi NP_001074881.1. NM_001081412.2.
UniGenei Mm.333722.

3D structure databases

ProteinModelPortali Q6PAJ1.
SMRi Q6PAJ1. Positions 5-66.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 225455. 10 interactions.
IntActi Q6PAJ1. 1 interaction.
STRINGi 10090.ENSMUSP00000039247.

PTM databases

PhosphoSitei Q6PAJ1.

Proteomic databases

MaxQBi Q6PAJ1.
PaxDbi Q6PAJ1.
PRIDEi Q6PAJ1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000164107 ; ENSMUSP00000126377 ; ENSMUSG00000009681 .
GeneIDi 110279.
KEGGi mmu:110279.
UCSCi uc007fqb.2. mouse.

Organism-specific databases

CTDi 613.
MGIi MGI:88141. Bcr.
Rougei Search...

Phylogenomic databases

eggNOGi NOG267747.
GeneTreei ENSGT00760000119098.
HOGENOMi HOG000006779.
HOVERGENi HBG004165.
InParanoidi Q6PAJ1.
KOi K08878.
OMAi KRANSHS.
OrthoDBi EOG7BP81P.
TreeFami TF105082.

Enzyme and pathway databases

Reactomei REACT_196460. Signaling by FGFR1 fusion mutants.
REACT_210090. Rho GTPase cycle.

Miscellaneous databases

ChiTaRSi BCR. mouse.
NextBioi 363683.
PROi Q6PAJ1.
SOURCEi Search...

Gene expression databases

Bgeei Q6PAJ1.
CleanExi MM_BCR.
ExpressionAtlasi Q6PAJ1. baseline and differential.
Genevestigatori Q6PAJ1.

Family and domain databases

Gene3Di 1.10.555.10. 1 hit.
1.20.900.10. 1 hit.
2.30.29.30. 2 hits.
2.60.40.150. 1 hit.
4.10.280.30. 1 hit.
InterProi IPR015123. Bcr-Abl_oncoprot_oligo.
IPR000008. C2_dom.
IPR000219. DH-domain.
IPR001331. GDS_CDC24_CS.
IPR001849. PH_domain.
IPR011993. PH_like_dom.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
[Graphical view ]
Pfami PF09036. Bcr-Abl_Oligo. 1 hit.
PF00168. C2. 1 hit.
PF00169. PH. 1 hit.
PF00620. RhoGAP. 1 hit.
PF00621. RhoGEF. 1 hit.
[Graphical view ]
SMARTi SM00239. C2. 1 hit.
SM00233. PH. 1 hit.
SM00324. RhoGAP. 1 hit.
SM00325. RhoGEF. 1 hit.
[Graphical view ]
SUPFAMi SSF48065. SSF48065. 1 hit.
SSF48350. SSF48350. 1 hit.
SSF49562. SSF49562. 1 hit.
SSF69036. SSF69036. 1 hit.
PROSITEi PS50004. C2. 1 hit.
PS00741. DH_1. 1 hit.
PS50010. DH_2. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS50238. RHOGAP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 212-1270.
    Strain: C57BL/6 and FVB/N.
    Tissue: Brain and Mammary gland.
  3. "Unique organization of the human BCR gene promoter."
    Zhu Q.S., Heisterkamp N., Groffen J.
    Nucleic Acids Res. 18:7119-7125(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 276-428.
  4. "Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H.
    DNA Res. 10:167-180(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 561-1270.
    Tissue: Embryonic tail.
  5. "Ccpg1, a novel scaffold protein that regulates the activity of the Rho guanine nucleotide exchange factor Dbs."
    Kostenko E.V., Olabisi O.O., Sahay S., Rodriguez P.L., Whitehead I.P.
    Mol. Cell. Biol. 26:8964-8975(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: POSSIBLE INTERACTION WITH CCPG1.
  6. "Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
    Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
    Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.
  7. "Quantitative time-resolved phosphoproteomic analysis of mast cell signaling."
    Cao L., Yu K., Banh C., Nguyen V., Ritz A., Raphael B.J., Kawakami Y., Kawakami T., Salomon A.R.
    J. Immunol. 179:5864-5876(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-237 AND TYR-247, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Mast cell.
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiBCR_MOUSE
AccessioniPrimary (citable) accession number: Q6PAJ1
Secondary accession number(s): E9PZL3
, Q61339, Q6ZPE5, Q99LW5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: July 27, 2011
Last modified: October 29, 2014
This is version 95 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3