Reviewed, UniProtKB/Swiss-Prot Q6PA41 (SYCM_XENLA)

Names and origin

Protein names

Recommended name:
    Probable cysteinyl-tRNA synthetase, mitochondrial
    EC=6.1.1.16
Alternative name(s):
    Cysteine--tRNA ligase
      Short name=CysRS

Gene names

Name:

cars2

Organism

Xenopus laevis (African clawed frog)

Taxonomic identifier

8355 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Mesobatrachia › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length	572 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys).
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subcellular location	Mitochondrion matrix By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Gene Ontology (GO)
Biological process	cysteinyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cysteine-tRNA ligase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 572		Probable cysteinyl-tRNA synthetase, mitochondrial		PRO_0000250742
Regions
	Motif	83 – 93	11	"HIGH" region
	Motif	320 – 324	5	"KMSKS" region
Sites
	Metal binding	81	1	Zinc By similarity
	Metal binding	260	1	Zinc By similarity
	Metal binding	285	1	Zinc By similarity
	Metal binding	289	1	Zinc By similarity
	Binding site	323	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6PA41-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: E3248143C332ABE4	FASTA	572	64,779
	10 20 30 40 50 60 MNTLMRRACR RALFPGSFRL PALDPLNGTL MFSSGNACAA PVGCWAPPSG QDTGIKVYNS 70 80 90 100 110 120 LTRKKEPLIL ADPTVATWYS CGPTVYDHAH LGHACSYVRF DTIRRILLKV FGIDIVMAMV 130 140 150 160 170 180 VTDIDDKIIK RAKELNISPV ALARTYEQDF KQDMTALRVL PPTVYMRVTE SIPQIISFIQ 190 200 210 220 230 240 QIIANGHAYS TSQGNVYFDV QSIGDQYGKF NDSFCDAASE SALQDKRHIR DFALWKTSKP 250 260 270 280 290 300 EEPYWASPWG KGRPGWHIEC STIASSVFGK HLDIHTGGID LAFPHHENEI AQCEAYHQST 310 320 330 340 350 360 QWGNYFLHTG HLHLKGNEEK MSKSLKNYVT IKEFLKSFSP DHLRMFCLRS KYKSAVEYSK 370 380 390 400 410 420 ESMHDAVNTL HLISSFVDDA KAYLKGQLIC QPVQETLLWQ RLNETKVNVK AAFSDDFDTP 430 440 450 460 470 480 RAVDAVLDLI HHGNRQLKAV SKESSSPRSP IVYGAMVSYI EQFLEIMGIS LGQNQVPAEE 490 500 510 520 530 540 RHSAMLFNVV EEMVSFRSKV RNYSLATNES ATAIGQEEKQ QYKERRRQLL LEREPLLQAC 550 560 570 DIARQHLAVY GINVKDRGDT STWELLDRKE ET

References

[1]	NIH - Xenopus Gene Collection (XGC) project Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC060463 mRNA. Translation: AAH60463.1.
RefSeq	NP_001083370.1.
UniGene	Xl.18697
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	398889.
KEGG	xla:398889.
Organism-specific databases
Xenbase	XB-FEAT-976542. cars2.
Phylogenomic databases
HOVERGEN	Q6PA41.
Enzyme and pathway databases
BRENDA	6.1.1.16. 648.
Family and domain databases
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR015804. Cys-tRNA-synt_Ia_C. IPR015803. Cys-tRNA-synt_Ia_N. IPR002308. Cys-tRNA-synth_1a. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR10890. Cys_tRNA-synt_1a. 1 hit.
Pfam	PF01406. tRNA-synt_1e. 1 hit. [Graphical view]
PRINTS	PR00983. TRNASYNTHCYS.
TIGRFAMs	TIGR00435. cysS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYCM_XENLA

Accession

Primary (citable) accession number: Q6PA41

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 3, 2006
Last sequence update:	July 5, 2004
Last modified:	June 16, 2009
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Xenopus annotation project