Q6P9X4 (TP4A2_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein tyrosine phosphatase type IVA 2
EC=3.1.3.48

Alternative name(s):
Protein-tyrosine phosphatase 4a2
Protein-tyrosine phosphatase of regenerating liver 2
Short name=PRL-2

Gene names

Name:	Ptp4a2
Synonyms:	Prl2

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	167 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Protein tyrosine phosphatase which stimulates progression from G1 into S phase during mitosis. Inhibits geranylgeranyl transferase type II activity by blocking the association between RABGGTA and RABGGTB By similarity.
Catalytic activity	Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.
Enzyme regulation	Inhibited by sodium orthovanadate and pentamidine By similarity.
Subunit structure	In contrast to PTP4A1 and PTP4A3, does not interact with tubulin. Interacts with RABGGTB By similarity.
Subcellular location	Cell membrane By similarity. Early endosome By similarity. Cytoplasm By similarity.
Tissue specificity	Anterior pituitary, liver, brain, adrenal gland, kidney, testis and heart. Expression in the anterior pituitary is 3 fold higher in male as compared to female. Ref.1
Post-translational modification	Farnesylated. Farnesylation is required for membrane targeting and for interaction with RABGGTB By similarity.
Sequence similarities	Belongs to the protein-tyrosine phosphatase family. Contains 1 tyrosine-protein phosphatase domain.

Ontologies

Keywords
Cellular component	Cell membrane Cytoplasm Endosome Membrane
Molecular function	Hydrolase Protein phosphatase
PTM	Disulfide bond Lipoprotein Methylation Prenylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	peptidyl-tyrosine dephosphorylation Inferred from electronic annotation. Source: GOC
Cellular_component	early endosome Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	protein tyrosine phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 164	164	Protein tyrosine phosphatase type IVA 2		PRO_0000094787
	Propeptide	165 – 167	3	Removed in mature form By similarity		PRO_0000396733
Regions
	Domain	79 – 145	67	Tyrosine-protein phosphatase
	Region	102 – 107	6	Phosphate binding By similarity
Sites
	Active site	69	1	Proton donor By similarity
	Active site	101	1	Phosphocysteine intermediate By similarity
	Binding site	107	1	Substrate By similarity
Amino acid modifications
	Modified residue	164	1	Cysteine methyl ester By similarity
	Lipidation	164	1	S-farnesyl cysteine By similarity
	Disulfide bond	46 ↔ 101		By similarity
Experimental info
	Sequence conflict	23	1	T → A in CAA07417. Ref.1
	Sequence conflict	157	1	F → S in CAA07417. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q6P9X4 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: E97B88BF87B87943	FASTA	167	19,127
	10 20 30 40 50 60 MNRPAPVEIS YENMRFLITH NPTNATLNKF TEELKKYGVT TLVRVCDATY DKAPVEKEGI 70 80 90 100 110 120 HVLDWPFDDG APPPNQIVDD WLNLLKTKFR EEPGCCVAVH CVAGLGRAPV LVALALIECG 130 140 150 160 MKYEDAVQFI RQKRRGAFNS KQLLYLEKYR PKMRLRFRDT NGHCCVQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Expression of a novel rat protein tyrosine phosphatase gene." Carter D.A. Biochim. Biophys. Acta 1442:405-408(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Strain: Sprague-Dawley. Tissue: Pituitary anterior lobe.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Pituitary.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ007016 mRNA. Translation: CAA07417.1. BC060549 mRNA. Translation: AAH60549.1.
IPI	IPI00212518.
RefSeq	NP_445927.1. NM_053475.1.
UniGene	Rn.167750.
3D structure databases
ProteinModelPortal	Q6P9X4.
ModBase	Search...
Proteomic databases
PRIDE	Q6P9X4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000074415; ENSRNOP00000064832; ENSRNOG00000050044.
GeneID	85237.
KEGG	rno:85237.
Organism-specific databases
CTD	8073.
RGD	619786. Ptp4a2.
Phylogenomic databases
GeneTree	ENSGT00390000009788.
HOVERGEN	HBG071295.
KO	K01104.
Gene expression databases
Genevestigator	Q6P9X4.
Family and domain databases
InterPro	IPR000387. Tyr/Dual-sp_Pase. IPR000242. Tyr_Pase_rcpt/non-rcpt. [Graphical view]
Pfam	PF00102. Y_phosphatase. 1 hit. [Graphical view]
PROSITE	PS00383. TYR_PHOSPHATASE_1. False negative. PS50056. TYR_PHOSPHATASE_2. 1 hit. PS50055. TYR_PHOSPHATASE_PTP. False negative. [Graphical view]
ProtoNet	Search...
Other
BindingDB	Q6P9X4.
NextBio	617278.

Entry information

Entry name

TP4A2_RAT

Accession

Primary (citable) accession number: Q6P9X4
Secondary accession number(s): O88765

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 5, 2005
Last sequence update:	July 5, 2004
Last modified:	May 1, 2013
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program