Impdh2

Functionⁱ

Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth. Could also have a single-stranded nucleic acid-binding activity and could play a role in RNA and/or DNA metabolism. It may also have a role in the development of malignancy and the growth progression of some tumors.UniRule annotation

Catalytic activityⁱ

Inosine 5'-phosphate + NAD⁺ + H₂O = xanthosine 5'-phosphate + NADH.UniRule annotation

Cofactorⁱ

K⁺UniRule annotation

Enzyme regulationⁱ

Mycophenolic acid (MPA) is a non-competitive inhibitor that prevents formation of the closed enzyme conformation by binding to the same site as the amobile flap. In contrast, mizoribine monophosphate (MZP) is a competitive inhibitor that induces the closed conformation. MPA is a potent inhibitor of mammalian IMPDHs but a poor inhibitor of the bacterial enzymes. MZP is a more potent inhibitor of bacterial IMPDH.UniRule annotation

Pathwayⁱ: XMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes XMP from IMP.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:

Inosine-5'-monophosphate dehydrogenase (Impdh1), Inosine-5'-monophosphate dehydrogenase (Impdh2), Inosine-5'-monophosphate dehydrogenase 2 (Impdh2), Inosine-5'-monophosphate dehydrogenase 1 (Impdh1)

This subpathway is part of the pathway XMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes XMP from IMP, the pathway XMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature key	Position(s)	DescriptionActions	Length
Metal bindingⁱ	326	Potassium; via carbonyl oxygenUniRule annotation	1
Metal bindingⁱ	328	Potassium; via carbonyl oxygenUniRule annotation	1
Binding siteⁱ	329	IMPUniRule annotation	1
Active siteⁱ	331	Thioimidate intermediateUniRule annotation	1
Metal bindingⁱ	331	Potassium; via carbonyl oxygenUniRule annotation	1
Active siteⁱ	429	Proton acceptorUniRule annotation	1
Binding siteⁱ	441	IMPUniRule annotation	1
Metal bindingⁱ	496	Potassium; via carbonyl oxygen; shared with tetrameric partnerUniRule annotation	1

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Nucleotide bindingⁱ	274 – 276	NADUniRule annotation		3
Nucleotide bindingⁱ	324 – 326	NADUniRule annotation		3

GO - Molecular functionⁱ

IMP dehydrogenase activity Source: UniProtKB-UniRule
metal ion binding Source: UniProtKB-UniRule
nucleotide binding Source: UniProtKB-UniRule

Complete GO annotation...

GO - Biological processⁱ

GMP biosynthetic process Source: UniProtKB-UniRule

Complete GO annotation...

Keywordsⁱ

Molecular function	OxidoreductaseUniRule annotation
Biological process	GMP biosynthesisUniRule annotation, Purine biosynthesis
Ligand	Metal-bindingUniRule annotation, NADUniRule annotation, PotassiumUniRule annotation

Enzyme and pathway databases

BRENDAⁱ	1.1.1.205. 5301.
SABIO-RKⁱ	Q6P9U9.
UniPathwayⁱ	UPA00601; UER00295.

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Inosine-5'-monophosphate dehydrogenaseUniRule annotation (EC:1.1.1.205UniRule annotation) Short name: IMP dehydrogenaseUniRule annotation Short name: IMPDUniRule annotation Short name: IMPDHUniRule annotation
Gene namesⁱ	Name:Impdh2Imported Synonyms:IMPDHUniRule annotation
Organismⁱ	Rattus norvegicus (Rat)Imported
Taxonomic identifierⁱ	10116 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus

Organism-specific databases

Rat genome database More...RGDⁱ	735092. Impdh2.

RGDⁱ

735092. Impdh2.

Keywords - Cellular componentⁱ

CytoplasmUniRule annotation, NucleusUniRule annotation

PTM / Processingⁱ

Proteomic databases

PaxDbⁱ	Q6P9U9.
PRIDEⁱ	Q6P9U9.

Expressionⁱ

Gene expression databases

Genevisibleⁱ	Q6P9U9. RN.

Genevisibleⁱ

Q6P9U9. RN.

Interactionⁱ

Subunit structureⁱ

Homotetramer.UniRule annotation

Protein-protein interaction databases

IntActⁱ	Q6P9U9. 1 interactor.

IntActⁱ

Q6P9U9. 1 interactor.

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q6P9U9.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	114 – 173	CBSInterPro annotationAdd BLAST		60
Domainⁱ	179 – 237	CBSInterPro annotationAdd BLAST		59

Region

Feature key	Position(s)	DescriptionActions	Length
Regionⁱ	364 – 366	IMP bindingUniRule annotation	3
Regionⁱ	387 – 388	IMP bindingUniRule annotation	2
Regionⁱ	411 – 415	IMP bindingUniRule annotation	5

Sequence similaritiesⁱ

Belongs to the IMPDH/GMPR family.UniRule annotation

Keywords - Domainⁱ

CBS domainPROSITE-ProRule annotation

Phylogenomic databases

eggNOGⁱ	KOG2550. Eukaryota. COG0516. LUCA. COG0517. LUCA.
HOGENOMⁱ	HOG000165752.
HOVERGENⁱ	HBG052122.
KEGG Orthology (KO) More...KOⁱ	K00088.
PhylomeDBⁱ	Q6P9U9.

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00381. IMPDH. 1 hit.
Gene3Dⁱ	3.20.20.70. 2 hits.
HAMAPⁱ	MF_01964. IMPDH. 1 hit.
InterProⁱ	View protein in InterPro IPR013785. Aldolase_TIM. IPR000644. CBS_dom. IPR005990. IMP_DH. IPR015875. IMP_DH/GMP_Rdtase_CS. IPR001093. IMP_DH_GMPRt.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00571. CBS. 2 hits. PF00478. IMPDH. 1 hit.
PIRSFⁱ	PIRSF000130. IMPDH. 1 hit.
SMARTⁱ	View protein in SMART SM00116. CBS. 2 hits.
TIGRFAMsⁱ	TIGR01302. IMP_dehydrog. 1 hit.
PROSITEⁱ	View protein in PROSITE PS51371. CBS. 2 hits. PS00487. IMP_DH_GMP_RED. 1 hit.

Sequenceⁱ

Sequence statusⁱ: Complete.

Q6P9U9-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MADYLISGGT SYVPDDGLTA QQLFNCGDGL TYNDFLILPG YIDFTADQVD 
        60         70         80         90        100
LTSALTKKIT LKTPLVSSPM DTVTEAGMAI AMALTGGIGF IHHNCTPEFQ 
       110        120        130        140        150
ANEVRKVKKY EQGFITDPVV LSPKDRVRDV FEAKARHGFC GIPITDTGRM 
       160        170        180        190        200
GSRLVGIISS RDIDFLKEEE HDRFLEEIMT KREDLVVAPA GVTLKEANEI 
       210        220        230        240        250
LQRSKKGKLP IVNESDELVA IIARTDLKKN RDYPLASKDT KKQLLCGAAI 
       260        270        280        290        300
GTHEDDKYRL DLLALAGVDV VVLDSSQGNS IFQINMIKYI KEKYPNLQVI 
       310        320        330        340        350
GGNVVTAAQA KNLIDAGVDA LRVGMGSGSI CITQEVLASG RPQATAVYKV 
       360        370        380        390        400
SEYARRFGVP VIADGGIQNV GHIAKALALG ASTVMMGSLL AATTEAPGEY 
       410        420        430        440        450
FFSDGIRLKK YRGMGSLDAM DKHLSSQNRY FSEADKIKVA QGVSGAVQDK 
       460        470        480        490        500
GSIHKFVPYL IAGIQHSCQD IGAKSLTQVR AMMYSGELKF EKRTSSAQVE 
       510 
GGVHGLHSYE KRLF

Length:514

Mass (Da):55,799

Last modified:July 5, 2004 - v1

Checksum:ⁱFE85D44B8BEA2319

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	BC060585 mRNA. Translation: AAH60585.1.
NCBI Reference Sequences More...RefSeqⁱ	NP_954530.1. NM_199099.2.
UniGeneⁱ	Rn.8093.

Genome annotation databases

GeneIDⁱ	301005.
KEGGⁱ	rno:301005.
UCSC genome browser More...UCSCⁱ	RGD:735092. rat.

Similar proteinsⁱ

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
Q6P9U9	P12268 H2R178 G3RBQ8 UPI0000EE29EA UPI0008F52BE9 L8Y3Y1 UPI0003C8EA44 UPI0002744F75 UPI00029DB501 UPI0005F53B54 +184	Homo sapiens (Human) Pan troglodytes (Chimpanzee) Gorilla gorilla gorilla (Western lowland gorilla) Tupaia chinensis (Chinese tree shrew) Pan paniscus (Pygmy chimpanzee) (Bonobo) Colobus angolensis palliatus (Peters' Angolan colobus) Mandrillus leucophaeus (Drill) (Papio leucophaeus) Microtus ochrogaster (Prairie vole) Tarsius syrichta (Philippine tarsier) Xenopus tropicalis (Western clawed frog) (Silurana tropicalis) And more	514	UniRef90_P12268	Cluster: Inosine-5'-monophosphate dehydrogenase 2	195

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
Q6P9U9	P20839 P20839-6 UPI00005A7B12 A0A024R725 UPI0008F51685 UPI000511C739 UPI0004F04D34 A0A0D9RCH9 A0A096NUC2 K7BYR4 +676	Homo sapiens (Human) Gorilla gorilla gorilla (Western lowland gorilla) Pan troglodytes (Chimpanzee) Pan paniscus (Pygmy chimpanzee) (Bonobo) Chlorocebus sabaeus (Green monkey) (Cercopithecus sabaeus) Papio anubis (Olive baboon) Macaca mulatta (Rhesus macaque) Rhinopithecus bieti (Black snub-nosed monkey) (Pygathrix bieti) Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii) Callithrix jacchus (White-tufted-ear marmoset) And more	514	UniRef50_P20839	Cluster: Inosine-5'-monophosphate dehydrogenase 1	687

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	BC060585 mRNA. Translation: AAH60585.1.
NCBI Reference Sequences More...RefSeqⁱ	NP_954530.1. NM_199099.2.
UniGeneⁱ	Rn.8093.

3D structure databases

ProteinModelPortalⁱ	Q6P9U9.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

IntActⁱ	Q6P9U9. 1 interactor.

IntActⁱ

Q6P9U9. 1 interactor.

Proteomic databases

PaxDbⁱ	Q6P9U9.
PRIDEⁱ	Q6P9U9.

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

GeneIDⁱ	301005.
KEGGⁱ	rno:301005.
UCSC genome browser More...UCSCⁱ	RGD:735092. rat.

Organism-specific databases

CTDⁱ	3615.
Rat genome database More...RGDⁱ	735092. Impdh2.

Phylogenomic databases

eggNOGⁱ	KOG2550. Eukaryota. COG0516. LUCA. COG0517. LUCA.
HOGENOMⁱ	HOG000165752.
HOVERGENⁱ	HBG052122.
KEGG Orthology (KO) More...KOⁱ	K00088.
PhylomeDBⁱ	Q6P9U9.

Enzyme and pathway databases

UniPathwayⁱ	UPA00601; UER00295.
BRENDAⁱ	1.1.1.205. 5301.
SABIO-RKⁱ	Q6P9U9.

Gene expression databases

Genevisibleⁱ	Q6P9U9. RN.

Genevisibleⁱ

Q6P9U9. RN.

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00381. IMPDH. 1 hit.
Gene3Dⁱ	3.20.20.70. 2 hits.
HAMAPⁱ	MF_01964. IMPDH. 1 hit.
InterProⁱ	View protein in InterPro IPR013785. Aldolase_TIM. IPR000644. CBS_dom. IPR005990. IMP_DH. IPR015875. IMP_DH/GMP_Rdtase_CS. IPR001093. IMP_DH_GMPRt.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00571. CBS. 2 hits. PF00478. IMPDH. 1 hit.
PIRSFⁱ	PIRSF000130. IMPDH. 1 hit.
SMARTⁱ	View protein in SMART SM00116. CBS. 2 hits.
TIGRFAMsⁱ	TIGR01302. IMP_dehydrog. 1 hit.
PROSITEⁱ	View protein in PROSITE PS51371. CBS. 2 hits. PS00487. IMP_DH_GMP_RED. 1 hit.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	Q6P9U9_RAT
Accessionⁱ	Q6P9U9Primary (citable) accession number: Q6P9U9
Entry historyⁱ	Integrated into UniProtKB/TrEMBL:	July 5, 2004
	Last sequence update:	July 5, 2004
	Last modified:	September 27, 2017
	This is version 111 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Unreviewed (UniProtKB/TrEMBL)

Miscellaneousⁱ

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q6P9U9 (Q6P9U9_RAT)

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Inosine-5'-monophosphate dehydrogenase

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the ‘Function’ section describes an enzyme regulatory mechanism and reports the components which regulate (by activation or inhibition) the reaction.<p><a href='/help/enzyme_regulation' target='_top'>More...</a></p>Enzyme regulationi

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">‘Function’</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: XMP biosynthesis via de novo pathway

Sites

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Other locations

Nucleus

Other locations

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Proteomic databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Region

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including length and molecular weight.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Caution

Functionⁱ

Enzyme regulationⁱ

Pathwayⁱ: XMP biosynthesis via de novo pathway

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Sequenceⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ