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Protein

Rho guanine nucleotide exchange factor 18

Gene

Arhgef18

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPases. May play a role in actin cytoskeleton reorganization in different tissues since its activation induces formation of actin stress fibers. Also act as a GEF for RAC1, inducing production of reactive oxygen species (ROS). Does not act as a GEF for CDC42. The G protein beta-gamma (Gbetagamma) subunits of heterotrimeric G proteins act as activators, explaining the integrated effects of LPA and other G-protein coupled receptor agonists on actin stress fiber formation, cell shape change and ROS production. Required for EPB41L4B-mediated regulation of the circumferential actomyosin belt in epithelial cells.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGuanine-nucleotide releasing factor

Enzyme and pathway databases

ReactomeiR-MMU-193648 NRAGE signals death through JNK
R-MMU-194840 Rho GTPase cycle
R-MMU-2173791 TGF-beta receptor signaling in EMT (epithelial to mesenchymal transition)
R-MMU-416482 G alpha (12/13) signalling events

Names & Taxonomyi

Protein namesi
Recommended name:
Rho guanine nucleotide exchange factor 18
Gene namesi
Name:Arhgef18
Synonyms:Kiaa0521
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:2142567 Arhgef18

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoskeleton, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003414161 – 1021Rho guanine nucleotide exchange factor 18Add BLAST1021

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei568PhosphothreonineCombined sources1
Modified residuei577PhosphoserineCombined sources1
Modified residuei952PhosphoserineCombined sources1
Modified residuei954PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ6P9R4
PeptideAtlasiQ6P9R4
PRIDEiQ6P9R4

PTM databases

iPTMnetiQ6P9R4
PhosphoSitePlusiQ6P9R4

Expressioni

Gene expression databases

BgeeiENSMUSG00000004568
ExpressionAtlasiQ6P9R4 baseline and differential
GenevisibleiQ6P9R4 MM

Interactioni

Subunit structurei

Interacts with SEPT9; interaction may inhibit GEF activity. Interacts with Gbetagamma subunits GNB1 and GNG2 (By similarity). Interacts with EPB41L4B. Interacts with PATJ (via C-terminus).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi221794, 4 interactors
IntActiQ6P9R4, 4 interactors
STRINGi10090.ENSMUSP00000004684

Structurei

Secondary structure

11021
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi304 – 314Combined sources11
Beta strandi320 – 322Combined sources3
Beta strandi328 – 330Combined sources3
Helixi331 – 334Combined sources4
Beta strandi340 – 349Combined sources10
Beta strandi355 – 373Combined sources19
Beta strandi376 – 379Combined sources4
Beta strandi387 – 390Combined sources4
Beta strandi395 – 399Combined sources5
Beta strandi402 – 410Combined sources9
Beta strandi417 – 422Combined sources6
Helixi426 – 441Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
6BCBX-ray1.40A302-444[»]
ProteinModelPortaliQ6P9R4
SMRiQ6P9R4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini101 – 298DHPROSITE-ProRule annotationAdd BLAST198
Domaini339 – 441PHPROSITE-ProRule annotationAdd BLAST103

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili700 – 800Sequence analysisAdd BLAST101

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG3520 Eukaryota
COG5422 LUCA
GeneTreeiENSGT00760000119193
HOGENOMiHOG000236361
HOVERGENiHBG104846
InParanoidiQ6P9R4
KOiK21066
OMAiQFRLQST
OrthoDBiEOG091G00G7
TreeFamiTF325887

Family and domain databases

CDDicd15794 PH_ARHGEF18, 1 hit
cd00160 RhoGEF, 1 hit
Gene3Di1.20.900.10, 1 hit
2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR037744 ARHGEF18_PH
IPR035899 DBL_dom_sf
IPR000219 DH-domain
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
PfamiView protein in Pfam
PF00169 PH, 1 hit
PF00621 RhoGEF, 1 hit
SMARTiView protein in SMART
SM00233 PH, 1 hit
SM00325 RhoGEF, 1 hit
SUPFAMiSSF48065 SSF48065, 1 hit
PROSITEiView protein in PROSITE
PS50010 DH_2, 1 hit
PS50003 PH_DOMAIN, 1 hit

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6P9R4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTISQKGGLQ PTPSPAGSGV RLGPIAGDMD EADSVFLKLK QTADDSLSLT
60 70 80 90 100
SSNAESVFIE DPYIASLRCE IESDAHEFEA ESWSLSVDLA YAKKQKKEVV
110 120 130 140 150
KRQDVLYELM QTEAHHVRTL KIMLKVYSRA LQEELQFSGQ AVSRLFPCAD
160 170 180 190 200
DLLDMHSHFL ARLKERRQEF LEEGSDRNYV IQKIGDVLVQ QFSGETGERM
210 220 230 240 250
KEKYAVFCSG HNDAVGQYKL LLQQSKKFQN LIKKIGNFSI VRRLGVQECI
260 270 280 290 300
LLVTQRITKY PVLVERIIQN TEAGTEDYKD LSQALSLIKD IISQVDAKVS
310 320 330 340 350
EYEKDQRLKE IAAKTDQKSS GKLKNGLTFR KEDMLQQRQL HLEGALCWKS
360 370 380 390 400
TSGRLKDVLA VLLTDVLLLL QEKDQKYVFA SVDSKPPVIS LQKLIVREVA
410 420 430 440 450
NEEKAMFLIS ASMQGPEMYE MYTSSKEDRN IWMAHIRRAV ESCPDEEEDV
460 470 480 490 500
FSEAEEKKIA EARTMKLQEF QERLSLKDQL IAQSLLEKQQ IYLEMAQLSG
510 520 530 540 550
LEESAQNRGL FRGGGDPSET LRGEQILRSA MSEIEGIQSL ICQRHLGSTS
560 570 580 590 600
SQVEEGSVSA GLPRRAETFG GYDSVGSPSK GGSFKRKVSN SDLRPQDWQG
610 620 630 640 650
PASSPDSRPC DNSAPSGCCE ESPQAVEMPS TESLPTVLEL ELVHRVQTLS
660 670 680 690 700
QLLLSLQAVI AQQDSYVEMQ RTAIQEREKQ FRLQSTRGNL LLEQERQRNF
710 720 730 740 750
EKQREERAGV EKLQSQLRQE QQRWERERAR QQQELELAGA RLQEREGEAR
760 770 780 790 800
QMRQRLDQER TELERQRQAY QHDLERLREA QRAVDRERER LELLRRFKKQ
810 820 830 840 850
NTVPGALPPE VLAEAQPASH PPSFNGDGLE GHSAPAKAPG TQGSAMLHGT
860 870 880 890 900
GPDNVERPEV ARWDSAPPES RPAKSDVPIQ LLSATNQIQR QTAVQQQIPT
910 920 930 940 950
KLAASTKGGK EKGSKSRGSQ RWESSASFDL KQQLLLSKFI GKDESASRNR
960 970 980 990 1000
RSLSPVLPAA HGSAPASDPC FPAPSPAPAA TPPEAFKFGG TSLPPVSPAS
1010 1020
SLPTTPLATT DEVSKEDVIF F
Length:1,021
Mass (Da):114,336
Last modified:July 27, 2011 - v2
Checksum:iC62C35875FE1FA89
GO
Isoform 2 (identifier: Q6P9R4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     926-1021: ASFDLKQQLL...EVSKEDVIFF → GELHPTPTTQ...QLHFCCDKIP

Note: No experimental confirmation available.
Show »
Length:983
Mass (Da):111,026
Checksum:i1F8C1006BAF352C3
GO
Isoform 3 (identifier: Q6P9R4-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     534-618: IEGIQSLICQ...PCDNSAPSGC → SKLASPLSYA...SGVGGGGDKA
     619-1021: Missing.

Note: No experimental confirmation available.
Show »
Length:618
Mass (Da):69,228
Checksum:iA0B550CC80D6FE32
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti53N → S in BAC30940 (PubMed:16141072).Curated1
Sequence conflicti217Q → H in AAH60639 (PubMed:15489334).Curated1
Sequence conflicti967S → P in AAH34512 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_034317534 – 618IEGIQ…APSGC → SKLASPLSYAMIFQGGLPGG PKPGSVLYCSPRTTVILLFF IIQGMESSMCNGSTAELYPN PLSTYSTNAPWVTLCSGVGG GGDKA in isoform 3. 1 PublicationAdd BLAST85
Alternative sequenceiVSP_034318619 – 1021Missing in isoform 3. 1 PublicationAdd BLAST403
Alternative sequenceiVSP_034319926 – 1021ASFDL…DVIFF → GELHPTPTTQRSCTPLPVDL SQQHIWNADREADRQAPVCA RRQRGSLFQLHFCCDKIP in isoform 2. 1 PublicationAdd BLAST96

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK041423 mRNA Translation: BAC30940.1
AC169677 Genomic DNA No translation available.
BC034512 mRNA Translation: AAH34512.1
BC060639 mRNA Translation: AAH60639.1
AK172963 mRNA Translation: BAD32241.1
CCDSiCCDS22060.1 [Q6P9R4-1]
RefSeqiNP_598723.3, NM_133962.3 [Q6P9R4-1]
UniGeneiMm.170461

Genome annotation databases

EnsembliENSMUST00000004684; ENSMUSP00000004684; ENSMUSG00000004568 [Q6P9R4-1]
GeneIDi102098
KEGGimmu:102098
UCSCiuc009kri.1 mouse [Q6P9R4-3]
uc009krj.1 mouse [Q6P9R4-1]
uc009krk.1 mouse [Q6P9R4-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiARHGI_MOUSE
AccessioniPrimary (citable) accession number: Q6P9R4
Secondary accession number(s): E9QK59
, Q6A055, Q8BYA4, Q8K227
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: July 27, 2011
Last modified: May 23, 2018
This is version 114 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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