Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

FK506-binding protein 15

Gene

Fkbp15

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the transport of early endosomes at the level of transition between microfilament-based and microtubule-based movement (By similarity). May be involved in the cytoskeletal organization of neuronal growth cones. Seems to be inactive as a PPIase.By similarity1 Publication

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Endocytosis, Transport

Keywords - Ligandi

Actin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
FK506-binding protein 15
Short name:
FKBP-15
Alternative name(s):
133 kDa FK506-binding protein
Short name:
133 kDa FKBP
Short name:
FKBP-133
WASP and FKBP-like
Short name:
WAFL
Gene namesi
Name:Fkbp15
Synonyms:Fkbp133, Kiaa0674
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:2444782. Fkbp15.

Subcellular locationi

GO - Cellular componenti

  • axon Source: MGI
  • early endosome Source: UniProtKB-SubCell
  • growth cone Source: MGI
  • membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cytoplasm, Endosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12161216FK506-binding protein 15PRO_0000299557Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei14 – 141PhosphoserineBy similarity
Modified residuei23 – 231PhosphoserineBy similarity
Modified residuei91 – 911N6-acetyllysineBy similarity
Modified residuei306 – 3061PhosphoserineCombined sources
Modified residuei310 – 3101PhosphoserineCombined sources
Modified residuei344 – 3441PhosphoserineCombined sources
Modified residuei617 – 6171PhosphoserineCombined sources
Modified residuei948 – 9481PhosphoserineBy similarity
Modified residuei1018 – 10181PhosphoserineBy similarity
Modified residuei1050 – 10501PhosphoserineCombined sources
Modified residuei1091 – 10911PhosphoserineCombined sources
Modified residuei1093 – 10931PhosphothreonineCombined sources
Modified residuei1108 – 11081PhosphoserineBy similarity
Modified residuei1153 – 11531PhosphoserineBy similarity
Modified residuei1157 – 11571PhosphoserineCombined sources
Modified residuei1159 – 11591PhosphoserineCombined sources
Modified residuei1190 – 11901PhosphoserineCombined sources
Modified residuei1198 – 11981PhosphothreonineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ6P9Q6.
MaxQBiQ6P9Q6.
PaxDbiQ6P9Q6.
PRIDEiQ6P9Q6.

PTM databases

iPTMnetiQ6P9Q6.
PhosphoSiteiQ6P9Q6.

Expressioni

Tissue specificityi

Expressed in brain, with highest levels in the granular cell layer of cerebellum and in the granule cell layer of dentate gyrus.1 Publication

Developmental stagei

Strongly expressed in the developing nervous system at E18.5. Present in brain, heart, lung, kidney and thymus at E18.5 (at protein level).1 Publication

Gene expression databases

BgeeiQ6P9Q6.
CleanExiMM_FKBP15.
ExpressionAtlasiQ6P9Q6. baseline and differential.
GenevisibleiQ6P9Q6. MM.

Interactioni

Subunit structurei

Interacts with WIP and actin.By similarity

Protein-protein interaction databases

BioGridi237211. 1 interaction.
IntActiQ6P9Q6. 1 interaction.
STRINGi10090.ENSMUSP00000081575.

Structurei

3D structure databases

ProteinModelPortaliQ6P9Q6.
SMRiQ6P9Q6. Positions 196-289.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini196 – 28994PPIase FKBP-typePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni71 – 16898Important for function in growth cone organizationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili519 – 790272Sequence analysisAdd
BLAST
Coiled coili820 – 86546Sequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi929 – 94113Glu-richAdd
BLAST
Compositional biasi944 – 1045102Pro-richAdd
BLAST
Compositional biasi1203 – 12119Poly-Asp

Domaini

The PPIase FKBP-type domain seems to be inactive both for FK506-binding and enzymatic activity.
The central coiled-coil region is responsible for association with early endosomes.By similarity

Sequence similaritiesi

Belongs to the FKBP-type PPIase family.Curated
Contains 1 PPIase FKBP-type domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410INYI. Eukaryota.
ENOG4112APP. LUCA.
GeneTreeiENSGT00530000064286.
HOGENOMiHOG000112601.
HOVERGENiHBG067251.
InParanoidiQ6P9Q6.
KOiK17478.
OMAiAKCEHLL.
OrthoDBiEOG75XGKG.
PhylomeDBiQ6P9Q6.
TreeFamiTF328592.

Family and domain databases

InterProiIPR001179. PPIase_FKBP_dom.
[Graphical view]
PfamiPF00254. FKBP_C. 1 hit.
[Graphical view]
PROSITEiPS50059. FKBP_PPIASE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform A (identifier: Q6P9Q6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MFGAGDEDDT DFLSPSGGAK LASLFGLDQA TMGHGNEFFQ YTAPKQPKKG
60 70 80 90 100
QGTAAGNQTA PKPAPATTGT SSVLFATAVH AYRYINGQYA KQGKFGAAVL
110 120 130 140 150
GNHTSREYRI LLYISQQQPV TVATIHLNFE LMVRPNNYST FYDDQRQNWS
160 170 180 190 200
IMFESEKAAV SFNKQVCVAK CNSISSLDAV LCQDLVAAEG PAVETGDSLE
210 220 230 240 250
VAYTGWLLQN HVLGQVFDST ANKDKPLRLK LGSGKVVKGL EDGLLGMKKG
260 270 280 290 300
GKRLIITPSA CAAGSEGVIG WTQPTDSILV FEVEVRRVKF ARDSGSDGHS
310 320 330 340 350
VSSRDSAAPS PIPASDSLSA DPVVTPLPLP LKPGEPGLRS KSNSLSEQLT
360 370 380 390 400
VNSNPDTVKA KLISRMAKMG QPMLPILPPQ LDSNDSETED ATVLRGAGQS
410 420 430 440 450
LVTPSIQPSL QPAHPVLPQM ASQAPQPSGS GLQTPSAALM QAVSLDSHSA
460 470 480 490 500
VSGNAQNFQP YAGVQAYAYP QTPSVTSQLQ PVRPLYPAPL SQAPHFQGSG
510 520 530 540 550
DMMSFLMTEA RQHNTEIRMA VNKVADKMDH LMTKVEELQK HSSGNSMLLP
560 570 580 590 600
SMSVTMETSM IMSNIQRIIQ ENERLKQELL EKSSRIEEQN DKISDLIERN
610 620 630 640 650
QRYVEQSNLM MEKRNNSLQT ATENTQARIL HAEQEKAKVT EELAAATAQV
660 670 680 690 700
SHLQLKMTAH QKKETELQLQ LTDNLKETDL LRGHVTRLQA DLSELREASE
710 720 730 740 750
QTQTKFKSEK QSRRQLELKV TSLEEELTDL RAEKTSLEKN LSERKKKSAQ
760 770 780 790 800
ERCQAEAEMD EIRKSHQEEL DRLRQLLKKA RVSTDQAAAE QLTLAQAELQ
810 820 830 840 850
SQWEAKCEQL LASARDEHLQ QYREVCAQRD AHQQKLALLQ DECLALQAQI
860 870 880 890 900
AAFTEQKEHM QRLEKTKSQA PAGRAAADPS EKVKKIMNQV FQSLRGEFEL
910 920 930 940 950
EESYDGGTIL RTIMHTIKMV TLQLLNHQEE EEEEEEEEEE EKKPLRPSLE
960 970 980 990 1000
QPGPATPGMP PAPPSGETQE APEVLPEQVV GETTPLPLQA LPTPENGAQT
1010 1020 1030 1040 1050
RKGEPAEAEV PSEIKDSSLP PQPAGIPAHR VLGPPTSIPP KPPGPVTMDS
1060 1070 1080 1090 1100
ESEEMLAADQ RTVQPNGLLG EEHVREVATD GLLQGNSRRL SLTPDPEKGE
1110 1120 1130 1140 1150
PPALDPESQG GEAQPPECKQ AEDVSSSGPR ETLLDTELAS AAAGTSLRHN
1160 1170 1180 1190 1200
QDSQHCSLSG DEEDELFKGA TLKVPRPTAQ PEEEDEDEVS MKGRPPPTPL
1210
FGDDDDDDDD DIGWLG
Length:1,216
Mass (Da):132,961
Last modified:September 11, 2007 - v2
Checksum:i8F8130D5038122FF
GO
Isoform B (identifier: Q6P9Q6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1190-1216: SMKGRPPPTPLFGDDDDDDDDDIGWLG → VRRPQALLSP...DGDGQLPLYS

Show »
Length:1,255
Mass (Da):137,156
Checksum:i5146F31FFB4CEEC4
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti652 – 6521H → R in BAE36541 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1190 – 121627SMKGR…IGWLG → VRRPQALLSPHTTTWGLFPA GSETRKGVCWWLVSWTLCTG PCGSKLGAQSGQLCKHDGDG QLPLYS in isoform B. 1 PublicationVSP_027759Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL683829, AL732548 Genomic DNA. Translation: CAI25942.1.
AL732548, AL683829 Genomic DNA. Translation: CAI26145.1.
BC060651 mRNA. Translation: AAH60651.1.
AK161705 mRNA. Translation: BAE36541.1.
AK122343 mRNA. Translation: BAC65625.1.
CCDSiCCDS51201.1. [Q6P9Q6-1]
RefSeqiNP_001038993.1. NM_001045528.1. [Q6P9Q6-1]
XP_006538097.1. XM_006538034.2. [Q6P9Q6-2]
UniGeneiMm.357792.

Genome annotation databases

EnsembliENSMUST00000084527; ENSMUSP00000081575; ENSMUSG00000066151. [Q6P9Q6-1]
GeneIDi338355.
KEGGimmu:338355.
UCSCiuc008teh.2. mouse. [Q6P9Q6-1]
uc008tei.1. mouse. [Q6P9Q6-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL683829, AL732548 Genomic DNA. Translation: CAI25942.1.
AL732548, AL683829 Genomic DNA. Translation: CAI26145.1.
BC060651 mRNA. Translation: AAH60651.1.
AK161705 mRNA. Translation: BAE36541.1.
AK122343 mRNA. Translation: BAC65625.1.
CCDSiCCDS51201.1. [Q6P9Q6-1]
RefSeqiNP_001038993.1. NM_001045528.1. [Q6P9Q6-1]
XP_006538097.1. XM_006538034.2. [Q6P9Q6-2]
UniGeneiMm.357792.

3D structure databases

ProteinModelPortaliQ6P9Q6.
SMRiQ6P9Q6. Positions 196-289.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi237211. 1 interaction.
IntActiQ6P9Q6. 1 interaction.
STRINGi10090.ENSMUSP00000081575.

PTM databases

iPTMnetiQ6P9Q6.
PhosphoSiteiQ6P9Q6.

Proteomic databases

EPDiQ6P9Q6.
MaxQBiQ6P9Q6.
PaxDbiQ6P9Q6.
PRIDEiQ6P9Q6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000084527; ENSMUSP00000081575; ENSMUSG00000066151. [Q6P9Q6-1]
GeneIDi338355.
KEGGimmu:338355.
UCSCiuc008teh.2. mouse. [Q6P9Q6-1]
uc008tei.1. mouse. [Q6P9Q6-2]

Organism-specific databases

CTDi23307.
MGIiMGI:2444782. Fkbp15.
RougeiSearch...

Phylogenomic databases

eggNOGiENOG410INYI. Eukaryota.
ENOG4112APP. LUCA.
GeneTreeiENSGT00530000064286.
HOGENOMiHOG000112601.
HOVERGENiHBG067251.
InParanoidiQ6P9Q6.
KOiK17478.
OMAiAKCEHLL.
OrthoDBiEOG75XGKG.
PhylomeDBiQ6P9Q6.
TreeFamiTF328592.

Miscellaneous databases

ChiTaRSiFkbp15. mouse.
NextBioi400129.
PROiQ6P9Q6.
SOURCEiSearch...

Gene expression databases

BgeeiQ6P9Q6.
CleanExiMM_FKBP15.
ExpressionAtlasiQ6P9Q6. baseline and differential.
GenevisibleiQ6P9Q6. MM.

Family and domain databases

InterProiIPR001179. PPIase_FKBP_dom.
[Graphical view]
PfamiPF00254. FKBP_C. 1 hit.
[Graphical view]
PROSITEiPS50059. FKBP_PPIASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A).
    Strain: C57BL/6J.
    Tissue: Brain.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-704.
    Strain: C57BL/6J.
    Tissue: Embryo.
  4. "Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S., Nakajima D., Nagase T., Ohara O., Koga H.
    DNA Res. 10:35-48(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 213-1216 (ISOFORM B).
    Tissue: Brain.
  5. Cited for: IDENTIFICATION, ALTERNATIVE SPLICING (ISOFORMS A AND B), FUNCTION, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1091; THR-1093 AND SER-1159, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  7. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-344 AND SER-1159, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
    Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
    Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.
  9. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-306; SER-310; SER-617; SER-1050; SER-1091; THR-1093; SER-1157; SER-1159 AND SER-1190, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiFKB15_MOUSE
AccessioniPrimary (citable) accession number: Q6P9Q6
Secondary accession number(s): Q3TSY4, Q5SQG3, Q80TU5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: September 11, 2007
Last modified: March 16, 2016
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.