Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Neurexin-3

Gene

Nrxn3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Neuronal cell surface protein that may be involved in cell recognition and cell adhesion. May mediate intracellular signaling (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi304CalciumBy similarity1
Metal bindingi321Calcium; via carbonyl oxygenBy similarity1
Metal bindingi374Calcium; via carbonyl oxygenBy similarity1

GO - Molecular functioni

  • calcium channel regulator activity Source: MGI
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • adult behavior Source: Ensembl
  • cell adhesion Source: UniProtKB-KW
  • chemical synaptic transmission Source: MGI
  • learning Source: Ensembl
  • neurotransmitter secretion Source: MGI
  • positive regulation of synapse assembly Source: InterPro
  • positive regulation of synapse maturation Source: InterPro
  • social behavior Source: Ensembl
  • synapse assembly Source: MGI
  • vocalization behavior Source: Ensembl
Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-6794361. Interactions of neurexins and neuroligins at synapses.

Names & Taxonomyi

Protein namesi
Recommended name:
Neurexin-3
Alternative name(s):
Neurexin III-alpha
Neurexin-3-alpha
Gene namesi
Name:Nrxn3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:1096389. Nrxn3.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini28 – 1496ExtracellularSequence analysisAdd BLAST1469
Transmembranei1497 – 1517HelicalSequence analysisAdd BLAST21
Topological domaini1518 – 1571CytoplasmicSequence analysisAdd BLAST54

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • presynaptic membrane Source: MGI
  • protein complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 27Sequence analysisAdd BLAST27
ChainiPRO_000041255728 – 1571Neurexin-3Add BLAST1544

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi58N-linked (GlcNAc...)Sequence analysis1
Glycosylationi105N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi202 ↔ 213By similarity
Disulfide bondi207 ↔ 222By similarity
Disulfide bondi224 ↔ 234By similarity
Disulfide bondi404 ↔ 440By similarity
Disulfide bondi610 ↔ 639By similarity
Disulfide bondi647 ↔ 658By similarity
Disulfide bondi652 ↔ 667By similarity
Disulfide bondi669 ↔ 679By similarity
Glycosylationi757N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi1018 ↔ 1046By similarity
Disulfide bondi1053 ↔ 1064By similarity
Disulfide bondi1058 ↔ 1073By similarity
Disulfide bondi1075 ↔ 1085By similarity
Glycosylationi1189N-linked (GlcNAc...)Sequence analysis1
Glycosylationi1287N-linked (GlcNAc...)Sequence analysis1
Glycosylationi1331N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ6P9K9.
PeptideAtlasiQ6P9K9.
PRIDEiQ6P9K9.

PTM databases

iPTMnetiQ6P9K9.
PhosphoSitePlusiQ6P9K9.

Expressioni

Tissue specificityi

Brain and arteries (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000066392.
ExpressionAtlasiQ6P9K9. baseline and differential.
GenevisibleiQ6P9K9. MM.

Interactioni

Subunit structurei

The laminin G-like domain 2 binds to NXPH1. Specific isoforms bind to alpha-dystroglycan. The cytoplasmic C-terminal region binds to CASK (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
PTPRTO145222EBI-7281557,EBI-728180From a different organism.

Protein-protein interaction databases

IntActiQ6P9K9. 2 interactors.
MINTiMINT-4115192.
STRINGi10090.ENSMUSP00000129678.

Structurei

Secondary structure

11571
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi1091 – 1104Combined sources14
Helixi1107 – 1109Combined sources3
Beta strandi1113 – 1123Combined sources11
Beta strandi1127 – 1137Combined sources11
Beta strandi1143 – 1149Combined sources7
Beta strandi1152 – 1162Combined sources11
Beta strandi1164 – 1167Combined sources4
Beta strandi1175 – 1177Combined sources3
Beta strandi1179 – 1186Combined sources8
Beta strandi1189 – 1194Combined sources6
Beta strandi1200 – 1202Combined sources3
Beta strandi1245 – 1252Combined sources8
Helixi1253 – 1255Combined sources3
Beta strandi1261 – 1268Combined sources8
Helixi1273 – 1278Combined sources6
Beta strandi1284 – 1293Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3MW4X-ray2.00A/B/C1090-1293[»]
ProteinModelPortaliQ6P9K9.
SMRiQ6P9K9.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ6P9K9.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini28 – 202Laminin G-like 1PROSITE-ProRule annotationAdd BLAST175
Domaini198 – 235EGF-like 1PROSITE-ProRule annotationAdd BLAST38
Domaini258 – 440Laminin G-like 2PROSITE-ProRule annotationAdd BLAST183
Domaini447 – 639Laminin G-like 3PROSITE-ProRule annotationAdd BLAST193
Domaini643 – 680EGF-like 2PROSITE-ProRule annotationAdd BLAST38
Domaini685 – 857Laminin G-like 4PROSITE-ProRule annotationAdd BLAST173
Domaini871 – 1046Laminin G-like 5PROSITE-ProRule annotationAdd BLAST176
Domaini1049 – 1086EGF-like 3PROSITE-ProRule annotationAdd BLAST38
Domaini1090 – 1290Laminin G-like 6PROSITE-ProRule annotationAdd BLAST201

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi1302 – 1328Thr-richAdd BLAST27

Sequence similaritiesi

Belongs to the neurexin family.Curated
Contains 3 EGF-like domains.PROSITE-ProRule annotation
Contains 6 laminin G-like domains.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3514. Eukaryota.
ENOG410XNU6. LUCA.
GeneTreeiENSGT00760000118991.
HOGENOMiHOG000230481.
HOVERGENiHBG052670.
InParanoidiQ6P9K9.
KOiK07377.
OMAiCLGLEFL.
OrthoDBiEOG091G00KC.
TreeFamiTF321302.

Family and domain databases

Gene3Di2.60.120.200. 6 hits.
InterProiIPR013320. ConA-like_dom.
IPR000742. EGF-like_dom.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR001791. Laminin_G.
IPR003585. Neurexin-like.
IPR027158. NRXN3.
IPR027789. Syndecan/Neurexin_dom.
[Graphical view]
PANTHERiPTHR10127:SF405. PTHR10127:SF405. 3 hits.
PfamiPF00008. EGF. 1 hit.
PF02210. Laminin_G_2. 6 hits.
PF01034. Syndecan. 1 hit.
[Graphical view]
SMARTiSM00294. 4.1m. 1 hit.
SM00181. EGF. 3 hits.
SM00282. LamG. 6 hits.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 7 hits.
PROSITEiPS00010. ASX_HYDROXYL. 1 hit.
PS50026. EGF_3. 3 hits.
PS50025. LAM_G_DOMAIN. 6 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative promoter usage and alternative splicing. AlignAdd to basket

Note: A number of isoforms, beta-type and alpha-type are produced by alternative promoter usage. Beta-type isoforms differ from alpha-type isoforms in their N-terminus.
Isoform 1a (identifier: Q6P9K9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSFTLHSVFF TLKVSIFLGS LVGLCLGLEF MGLPNQWARY LRWDASTRSD
60 70 80 90 100
LSFQFKTNVS TGLLLYLDDG GVCDFLCLSL VDGRVQLRFS MDCAETTVLS
110 120 130 140 150
NKQVNDSSWH FLMVSRDRVR TGLVIDGEGQ SGELRPQRPY MDVVSDLFLG
160 170 180 190 200
GVPADIRPSA LTLDGVQSMP GFKGLMLDLK YGNSEPRLLG SQSVQLEAEG
210 220 230 240 250
PCGERPCENG GICFLLDGHP TCDCSTTGYG GTLCSEDVSQ GPGLSHLMMS
260 270 280 290 300
EQAREENVAT FRGSEYLCYD LSQNPIQSSS DEITLSFKTW QRNGLILHTG
310 320 330 340 350
KSADYVNLAL KDGAVSLVIN LGSGAFEAIV EPVNGKFNDN AWHDVKVTRN
360 370 380 390 400
LRQVTISVDG ILTTTGYTQE DYTMLGSDDF FYVGGSPSTA DLPGSPVSNN
410 420 430 440 450
FMGCLKEVVY KNNDIRLELS RLARIGDTKM KIYGEVVFKC ENVATLDPIN
460 470 480 490 500
FETPEAYISL PKWNTKRMGS ISFDFRTTEP NGLILFTHGK PQERKDVRSQ
510 520 530 540 550
KNTKVDFFAV ELLDGNLYLL LDMGSGTIKV KATQKKANDG EWYHVDIQRD
560 570 580 590 600
GRSGTISVNS RRTPFTASGE SEILDLEGDM YLGGLPENRA GLILPTELWT
610 620 630 640 650
AMLNYGYVGC IRDLFIDGRS KNIRQLAEMQ NAAGVKSSCS RMSAKQCDSY
660 670 680 690 700
PCKNNAVCKD GWNRFICDCT GTGYWGRTCE REASILSYDG SMYMKVIMPM
710 720 730 740 750
VMHTEAEDVS FRFMSQRAYG LLVATTSRDS ADTLRLELDG GRVKLMVNLD
760 770 780 790 800
CIRINCNSSK GPETLYAGQK LNDNEWHTVR VVRRGKSLKL TVDDDVAEGT
810 820 830 840 850
MVGDHTRLEF HNIETGIMTE KRYISVVPSS FIGHLQSLMF NGLLYIDLCK
860 870 880 890 900
NGDIDYCELK ARFGLRNIIA DPVTFKTKSS YLTLATLQAY TSMHLFFQFK
910 920 930 940 950
TTSADGFILF NSGDGNDFIA VELVKGYIHY VFDLGNGPNV IKGNSDRPLN
960 970 980 990 1000
DNQWHNVVIT RDSSNTHSLK VDTKVVTQVI NGAKNLDLKG DLYMAGLAQG
1010 1020 1030 1040 1050
MYSNLPKLVA SRDGFQGCLA SVDLNGRLPD LINDALHRSG QIERGCEGPS
1060 1070 1080 1090 1100
TTCQEDSCAN QGVCMQQWEG FTCDCSMTSY SGNQCNDPGA TYIFGKSGGL
1110 1120 1130 1140 1150
ILYTWPANDR PSTRSDRLAV GFSTTVKDGI LVRIDSAPGL GDFLQLHIEQ
1160 1170 1180 1190 1200
GKIGVVFNIG TVDISIKEER TPVNDGKYHV VRFTRNGGNA TLQVDNWPVN
1210 1220 1230 1240 1250
EHYPTGNTDN ERLQMVKQKI PFKYNRPVEE WLQEKGRQLT IFNTQAQIAI
1260 1270 1280 1290 1300
GGKDKGRLFQ GQLSGLYYDG LKVLNMAAEN NPNIKINGSV RLVGEVPSVS
1310 1320 1330 1340 1350
GTTQTTSMPP EMSTTVMETT TTMATTTTRK NRSTASIQPT SDDLVSSAEC
1360 1370 1380 1390 1400
SSDDEDFVEC EPSTDKSLST SIFEGGYKAH APKWESKDFR PNKVSETSRT
1410 1420 1430 1440 1450
TTTSLSPELI RFTASSSSGM VPKLPAGKMN NRDLKPQPDI VLLPLPTAYE
1460 1470 1480 1490 1500
LDSTKLKSPL ITSPMFRNVP TANPTEPGIR RVPGASEVIR ESSSTTGMVV
1510 1520 1530 1540 1550
GIVAAAALCI LILLYAMYKY RNRDEGSYQV DETRNYISNS AQSNGTLMKE
1560 1570
KQASSKSGHK KQKNKDKEYY V
Length:1,571
Mass (Da):173,428
Last modified:December 14, 2011 - v2
Checksum:i98F989F5DD2B71AB
GO
Isoform 2a (identifier: Q6P9K9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-373: Missing.
     1047-1047: E → EVALTKADLQ
     1364-1470: Missing.

Note: Produced by alternative splicing.
Show »
Length:1,100
Mass (Da):121,794
Checksum:iF2D6860CFAF3C8F0
GO
Isoform 1b (identifier: Q8C985-1) [UniParc]FASTAAdd to basket
The sequence of this isoform can be found in the external entry Q8C985.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:567
Mass (Da):62,376
GO

Sequence cautioni

The sequence AAH60719 differs from that shown. Contains an insert which is not supported by any other transcript and which does not match with the genome.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti390A → T in BAC27716 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0417101 – 373Missing in isoform 2a. 1 PublicationAdd BLAST373
Alternative sequenceiVSP_0417111047E → EVALTKADLQ in isoform 2a. 1 Publication1
Alternative sequenceiVSP_0417121364 – 1470Missing in isoform 2a. 1 PublicationAdd BLAST107

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK032126 mRNA. Translation: BAC27716.1.
AC115709 Genomic DNA. No translation available.
AC115744 Genomic DNA. No translation available.
AC115910 Genomic DNA. No translation available.
AC120383 Genomic DNA. No translation available.
AC154495 Genomic DNA. No translation available.
AC155231 Genomic DNA. No translation available.
AC155274 Genomic DNA. No translation available.
AC156637 Genomic DNA. No translation available.
AC161049 Genomic DNA. No translation available.
AC171335 Genomic DNA. No translation available.
CAAA01025627 Genomic DNA. No translation available.
CR974428 Genomic DNA. No translation available.
CR974583 Genomic DNA. No translation available.
CT009613 Genomic DNA. No translation available.
CT009725 Genomic DNA. No translation available.
CT010588 Genomic DNA. No translation available.
CU041252 Genomic DNA. No translation available.
BC060719 mRNA. Translation: AAH60719.1. Sequence problems.
CCDSiCCDS49134.1. [Q6P9K9-2]
CCDS56856.1. [Q6P9K9-1]
RefSeqiNP_001185516.2. NM_001198587.3. [Q6P9K9-1]
NP_766132.2. NM_172544.3. [Q6P9K9-2]
XP_006515620.1. XM_006515557.1. [Q6P9K9-1]
UniGeneiMm.425766.
Mm.487864.

Genome annotation databases

EnsembliENSMUST00000057634; ENSMUSP00000050075; ENSMUSG00000066392. [Q6P9K9-2]
ENSMUST00000163134; ENSMUSP00000129678; ENSMUSG00000066392. [Q6P9K9-1]
ENSMUST00000167887; ENSMUSP00000127926; ENSMUSG00000066392. [Q6P9K9-2]
GeneIDi18191.
KEGGimmu:18191.
UCSCiuc007okc.2. mouse. [Q6P9K9-2]
uc033ger.1. mouse. [Q6P9K9-1]

Keywords - Coding sequence diversityi

Alternative promoter usage, Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK032126 mRNA. Translation: BAC27716.1.
AC115709 Genomic DNA. No translation available.
AC115744 Genomic DNA. No translation available.
AC115910 Genomic DNA. No translation available.
AC120383 Genomic DNA. No translation available.
AC154495 Genomic DNA. No translation available.
AC155231 Genomic DNA. No translation available.
AC155274 Genomic DNA. No translation available.
AC156637 Genomic DNA. No translation available.
AC161049 Genomic DNA. No translation available.
AC171335 Genomic DNA. No translation available.
CAAA01025627 Genomic DNA. No translation available.
CR974428 Genomic DNA. No translation available.
CR974583 Genomic DNA. No translation available.
CT009613 Genomic DNA. No translation available.
CT009725 Genomic DNA. No translation available.
CT010588 Genomic DNA. No translation available.
CU041252 Genomic DNA. No translation available.
BC060719 mRNA. Translation: AAH60719.1. Sequence problems.
CCDSiCCDS49134.1. [Q6P9K9-2]
CCDS56856.1. [Q6P9K9-1]
RefSeqiNP_001185516.2. NM_001198587.3. [Q6P9K9-1]
NP_766132.2. NM_172544.3. [Q6P9K9-2]
XP_006515620.1. XM_006515557.1. [Q6P9K9-1]
UniGeneiMm.425766.
Mm.487864.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3MW4X-ray2.00A/B/C1090-1293[»]
ProteinModelPortaliQ6P9K9.
SMRiQ6P9K9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ6P9K9. 2 interactors.
MINTiMINT-4115192.
STRINGi10090.ENSMUSP00000129678.

PTM databases

iPTMnetiQ6P9K9.
PhosphoSitePlusiQ6P9K9.

Proteomic databases

PaxDbiQ6P9K9.
PeptideAtlasiQ6P9K9.
PRIDEiQ6P9K9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000057634; ENSMUSP00000050075; ENSMUSG00000066392. [Q6P9K9-2]
ENSMUST00000163134; ENSMUSP00000129678; ENSMUSG00000066392. [Q6P9K9-1]
ENSMUST00000167887; ENSMUSP00000127926; ENSMUSG00000066392. [Q6P9K9-2]
GeneIDi18191.
KEGGimmu:18191.
UCSCiuc007okc.2. mouse. [Q6P9K9-2]
uc033ger.1. mouse. [Q6P9K9-1]

Organism-specific databases

CTDi9369.
MGIiMGI:1096389. Nrxn3.

Phylogenomic databases

eggNOGiKOG3514. Eukaryota.
ENOG410XNU6. LUCA.
GeneTreeiENSGT00760000118991.
HOGENOMiHOG000230481.
HOVERGENiHBG052670.
InParanoidiQ6P9K9.
KOiK07377.
OMAiCLGLEFL.
OrthoDBiEOG091G00KC.
TreeFamiTF321302.

Enzyme and pathway databases

ReactomeiR-MMU-6794361. Interactions of neurexins and neuroligins at synapses.

Miscellaneous databases

ChiTaRSiNrxn3. mouse.
EvolutionaryTraceiQ6P9K9.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000066392.
ExpressionAtlasiQ6P9K9. baseline and differential.
GenevisibleiQ6P9K9. MM.

Family and domain databases

Gene3Di2.60.120.200. 6 hits.
InterProiIPR013320. ConA-like_dom.
IPR000742. EGF-like_dom.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR001791. Laminin_G.
IPR003585. Neurexin-like.
IPR027158. NRXN3.
IPR027789. Syndecan/Neurexin_dom.
[Graphical view]
PANTHERiPTHR10127:SF405. PTHR10127:SF405. 3 hits.
PfamiPF00008. EGF. 1 hit.
PF02210. Laminin_G_2. 6 hits.
PF01034. Syndecan. 1 hit.
[Graphical view]
SMARTiSM00294. 4.1m. 1 hit.
SM00181. EGF. 3 hits.
SM00282. LamG. 6 hits.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 7 hits.
PROSITEiPS00010. ASX_HYDROXYL. 1 hit.
PS50026. EGF_3. 3 hits.
PS50025. LAM_G_DOMAIN. 6 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNRX3A_MOUSE
AccessioniPrimary (citable) accession number: Q6P9K9
Secondary accession number(s): E9PW93, E9Q466, Q8CCT8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 21, 2011
Last sequence update: December 14, 2011
Last modified: November 2, 2016
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.