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Protein

Connector enhancer of kinase suppressor of ras 3

Gene

CNKSR3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in transepithelial sodium transport. Regulates aldosterone-induced and epithelial sodium channel (ENaC)-mediated sodium transport through regulation of ENaC cell surface expression. Acts as a scaffold protein coordinating the assembly of an ENaC-regulatory complex (ERC).1 Publication

GO - Biological processi

Names & Taxonomyi

Protein namesi
Recommended name:
Connector enhancer of kinase suppressor of ras 3
Short name:
Connector enhancer of KSR 3
Alternative name(s):
CNK homolog protein 3
Short name:
CNK3
CNKSR family member 3
Maguin-like protein
Gene namesi
Name:CNKSR3
Synonyms:MAGI1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

EuPathDBiHostDB:ENSG00000153721.17.
HGNCiHGNC:23034. CNKSR3.
MIMi617476. gene.
neXtProtiNX_Q6P9H4.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi154043.
OpenTargetsiENSG00000153721.
PharmGKBiPA134880153.

Polymorphism and mutation databases

BioMutaiCNKSR3.
DMDMi74758311.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003111051 – 555Connector enhancer of kinase suppressor of ras 3Add BLAST555

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei381PhosphoserineBy similarity1
Modified residuei383PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ6P9H4.
MaxQBiQ6P9H4.
PaxDbiQ6P9H4.
PeptideAtlasiQ6P9H4.
PRIDEiQ6P9H4.

PTM databases

iPTMnetiQ6P9H4.
PhosphoSitePlusiQ6P9H4.

Expressioni

Inductioni

Up-regulated by aldosterone (at protein level).1 Publication

Gene expression databases

BgeeiENSG00000153721.
CleanExiHS_CNKSR3.
HS_MAGI1.
ExpressionAtlasiQ6P9H4. baseline and differential.
GenevisibleiQ6P9H4. HS.

Organism-specific databases

HPAiCAB037133.
HPA049651.

Interactioni

Subunit structurei

Interacts with epithelial sodium channel ENaC. Interacts directly with SCNN1A (ENaC subunit alpha) and SCNN1B (ENaC subunit beta) C-terminal tails. Interacts with ENaC regulatory proteins NEDD4L, RAF1 and SGK1.1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi127531. 12 interactors.
IntActiQ6P9H4. 4 interactors.
STRINGi9606.ENSP00000356182.

Structurei

3D structure databases

ProteinModelPortaliQ6P9H4.
SMRiQ6P9H4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini7 – 72SAMPROSITE-ProRule annotationAdd BLAST66
Domaini80 – 174CRICPROSITE-ProRule annotationAdd BLAST95
Domaini211 – 293PDZPROSITE-ProRule annotationAdd BLAST83
Domaini325 – 546DUF1170Add BLAST222

Domaini

The PDZ domain is required for interaction with ENaC and SGK1, but not for interaction with NEDDL4 and RAF1.1 Publication

Sequence similaritiesi

Belongs to the CNKSR family.Curated

Phylogenomic databases

eggNOGiKOG1738. Eukaryota.
ENOG41104C7. LUCA.
GeneTreeiENSGT00390000017199.
HOGENOMiHOG000015752.
HOVERGENiHBG051040.
InParanoidiQ6P9H4.
OMAiKCKQPLP.
PhylomeDBiQ6P9H4.
TreeFamiTF326495.

Family and domain databases

InterProiView protein in InterPro
IPR010599. CNKSR2.
IPR017874. CRIC_domain.
IPR001478. PDZ.
IPR036034. PDZ_sf.
IPR001660. SAM.
IPR013761. SAM/pointed_sf.
PfamiView protein in Pfam
PF10534. CRIC_ras_sig. 1 hit.
PF06663. DUF1170. 1 hit.
PF00595. PDZ. 1 hit.
PF00536. SAM_1. 1 hit.
SMARTiView protein in SMART
SM00228. PDZ. 1 hit.
SM00454. SAM. 1 hit.
SUPFAMiSSF47769. SSF47769. 1 hit.
SSF50156. SSF50156. 1 hit.
PROSITEiView protein in PROSITE
PS51290. CRIC. 1 hit.
PS50106. PDZ. 1 hit.
PS50105. SAM_DOMAIN. 1 hit.

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform CNK3 (identifier: Q6P9H4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEPVTKWSPK QVVDWTRGLD DCLQQYVHKF EREKINGEQL LQISHQDLEE
60 70 80 90 100
LGVTRIGHQE LVLEAVDLLC ALNYGLETDN MKNLVLKLRA SSHNLQNYIS
110 120 130 140 150
SRRKSPAYDG NTSRKAPNEF LTSVVELIGA AKALLAWLDR APFTGITDFS
160 170 180 190 200
VTKNKIIQLC LDLTTTVQKD CFVAEMEDKV LTVVKVLNGI CDKTIRSTTD
210 220 230 240 250
PVMSQCACLE EVHLPNIKPG EGLGMYIKST YDGLHVITGT TENSPADRSQ
260 270 280 290 300
KIHAGDEVIQ VNQQTVVGWQ LKNLVKKLRE NPTGVVLLLK KRPTGSFNFT
310 320 330 340 350
PAPLKNLRWK PPLVQTSPPP ATTQSPESTM DTSLKKEKSA ILDLYIPPPP
360 370 380 390 400
AVPYSPRDEN GSFVYGGSSK CKQPLPGPKG SESPNSFLDQ ESRRRRFTIA
410 420 430 440 450
DSDQLPGYSV ETNILPTKMR EKTPSYGKPR PLSMPADGNW MGIVDPFARP
460 470 480 490 500
RGHGRKGEDA LCRYFSNERI PPIIEESSSP PYRFSRPTTE RHLVRGADYI
510 520 530 540 550
RGSRCYINSD LHSSATIPFQ EEGTKKKSGS SATKSSSTEP SLLVSWFTRL

KLLTH
Length:555
Mass (Da):61,904
Last modified:July 5, 2004 - v1
Checksum:i456E284566D5EFC8
GO
Isoform CNK3-IPCEF1-1 (identifier: G9CGD6-1) [UniParc]FASTAAdd to basket
Also known as: CNK3/IPCEF1 Long-1
The sequence of this isoform can be found in the external entry G9CGD6.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Note: Based on a naturally occurring readthrough transcript which produces an CNK3/IPCEF1 fusion protein.1 Publication
Length:899
Mass (Da):100,365
GO
Isoform CNK3-IPCEF1-2 (identifier: G9CGD6-2) [UniParc]FASTAAdd to basket
Also known as: CNK3/IPCEF1 Long-2
The sequence of this isoform can be found in the external entry G9CGD6.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Note: Based on a naturally occurring readthrough transcript which produces an CNK3/IPCEF1 fusion protein.1 Publication
Length:900
Mass (Da):100,493
GO
Isoform CNK3-IPCEF1-3 (identifier: G9CGD6-3) [UniParc]FASTAAdd to basket
Also known as: CNK3/IPCEF1 Short
The sequence of this isoform can be found in the external entry G9CGD6.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Note: Based on a naturally occurring readthrough transcript which produces an CNK3/IPCEF1 fusion protein. Major isoform found in CaCo2-cells.1 Publication
Length:830
Mass (Da):92,057
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti259I → T in BAB71044 (PubMed:14702039).Curated1
Sequence conflicti334L → P in AAQ92306 (Ref. 1) Curated1
Sequence conflicti334L → P in AAQ92307 (Ref. 1) Curated1
Sequence conflicti334L → P in AAQ92308 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY328891 mRNA. Translation: AAQ92306.1.
AY328892 mRNA. Translation: AAQ92307.1.
AY328893 mRNA. Translation: AAQ92308.1.
AK055911 mRNA. Translation: BAB71044.1.
AL357075 Genomic DNA. No translation available.
AL033376 Genomic DNA. No translation available.
AL590401 Genomic DNA. No translation available.
CH471051 Genomic DNA. Translation: EAW47699.1.
BC060761 mRNA. Translation: AAH60761.1.
CCDSiCCDS5246.1. [Q6P9H4-1]
RefSeqiNP_775786.2. NM_173515.3. [Q6P9H4-1]
UniGeneiHs.16064.

Genome annotation databases

EnsembliENST00000607772; ENSP00000475915; ENSG00000153721. [Q6P9H4-1]
GeneIDi154043.
KEGGihsa:154043.
UCSCiuc003qpy.4. human. [Q6P9H4-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiCNKR3_HUMAN
AccessioniPrimary (citable) accession number: Q6P9H4
Secondary accession number(s): C9IZX4, Q5SGD5, Q96N65
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: July 5, 2004
Last modified: February 28, 2018
This is version 131 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

The gene name MAGI1, shown in this entry as a synonym, is an obsolete human gene nomenclature committee-approved name. It should be noted that MAGI1 currently is the official name for the unrelated membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1.Curated

Keywords - Technical termi

Complete proteome, Reference proteome