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Protein

Connector enhancer of kinase suppressor of ras 3

Gene

CNKSR3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Probably involved in transepithelial sodium transport. Regulates aldosterone-induced and ENaC-mediated sodium transport possibly through regulation of the ERK pathway (By similarity).By similarity

GO - Biological processi

Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Connector enhancer of kinase suppressor of ras 3
Short name:
Connector enhancer of KSR 3
Alternative name(s):
CNK homolog protein 3
Short name:
CNK3
CNKSR family member 3
Maguin-like protein
Gene namesi
Name:CNKSR3
Synonyms:MAGI1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

HGNCiHGNC:23034. CNKSR3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134880153.

Polymorphism and mutation databases

BioMutaiCNKSR3.
DMDMi74758311.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 555555Connector enhancer of kinase suppressor of ras 3PRO_0000311105Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei381 – 3811PhosphoserineBy similarity
Modified residuei383 – 3831PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ6P9H4.
PaxDbiQ6P9H4.
PeptideAtlasiQ6P9H4.
PRIDEiQ6P9H4.

PTM databases

iPTMnetiQ6P9H4.
PhosphoSiteiQ6P9H4.

Expressioni

Inductioni

Up-regulated by aldosterone (at protein level).1 Publication

Gene expression databases

BgeeiQ6P9H4.
CleanExiHS_CNKSR3.
HS_MAGI1.
ExpressionAtlasiQ6P9H4. baseline and differential.
GenevisibleiQ6P9H4. HS.

Organism-specific databases

HPAiCAB037133.
HPA046557.
HPA049651.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
NMIQ132873EBI-10253274,EBI-372942
NMIQ8WTW23EBI-10253274,EBI-10174268

Protein-protein interaction databases

BioGridi127531. 9 interactions.
IntActiQ6P9H4. 3 interactions.
STRINGi9606.ENSP00000356182.

Structurei

3D structure databases

ProteinModelPortaliQ6P9H4.
SMRiQ6P9H4. Positions 2-76.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini7 – 7266SAMPROSITE-ProRule annotationAdd
BLAST
Domaini80 – 17495CRICPROSITE-ProRule annotationAdd
BLAST
Domaini211 – 29383PDZPROSITE-ProRule annotationAdd
BLAST
Domaini325 – 546222DUF1170Add
BLAST

Sequence similaritiesi

Belongs to the CNKSR family.Curated
Contains 1 CRIC domain.PROSITE-ProRule annotation
Contains 1 DUF1170 domain.Curated
Contains 1 PDZ (DHR) domain.PROSITE-ProRule annotation
Contains 1 SAM (sterile alpha motif) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1738. Eukaryota.
ENOG41104C7. LUCA.
GeneTreeiENSGT00390000017199.
HOGENOMiHOG000015752.
HOVERGENiHBG051040.
InParanoidiQ6P9H4.
OrthoDBiEOG71ZP0W.
PhylomeDBiQ6P9H4.
TreeFamiTF326495.

Family and domain databases

Gene3Di1.10.150.50. 1 hit.
2.30.42.10. 1 hit.
InterProiIPR010599. CNKSR2.
IPR017874. CRIC_domain.
IPR001478. PDZ.
IPR001660. SAM.
IPR013761. SAM/pointed.
[Graphical view]
PfamiPF10534. CRIC_ras_sig. 1 hit.
PF06663. DUF1170. 1 hit.
PF00595. PDZ. 1 hit.
PF00536. SAM_1. 1 hit.
[Graphical view]
SMARTiSM00228. PDZ. 1 hit.
SM00454. SAM. 1 hit.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 1 hit.
SSF50156. SSF50156. 1 hit.
PROSITEiPS51290. CRIC. 1 hit.
PS50106. PDZ. 1 hit.
PS50105. SAM_DOMAIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6P9H4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEPVTKWSPK QVVDWTRGLD DCLQQYVHKF EREKINGEQL LQISHQDLEE
60 70 80 90 100
LGVTRIGHQE LVLEAVDLLC ALNYGLETDN MKNLVLKLRA SSHNLQNYIS
110 120 130 140 150
SRRKSPAYDG NTSRKAPNEF LTSVVELIGA AKALLAWLDR APFTGITDFS
160 170 180 190 200
VTKNKIIQLC LDLTTTVQKD CFVAEMEDKV LTVVKVLNGI CDKTIRSTTD
210 220 230 240 250
PVMSQCACLE EVHLPNIKPG EGLGMYIKST YDGLHVITGT TENSPADRSQ
260 270 280 290 300
KIHAGDEVIQ VNQQTVVGWQ LKNLVKKLRE NPTGVVLLLK KRPTGSFNFT
310 320 330 340 350
PAPLKNLRWK PPLVQTSPPP ATTQSPESTM DTSLKKEKSA ILDLYIPPPP
360 370 380 390 400
AVPYSPRDEN GSFVYGGSSK CKQPLPGPKG SESPNSFLDQ ESRRRRFTIA
410 420 430 440 450
DSDQLPGYSV ETNILPTKMR EKTPSYGKPR PLSMPADGNW MGIVDPFARP
460 470 480 490 500
RGHGRKGEDA LCRYFSNERI PPIIEESSSP PYRFSRPTTE RHLVRGADYI
510 520 530 540 550
RGSRCYINSD LHSSATIPFQ EEGTKKKSGS SATKSSSTEP SLLVSWFTRL

KLLTH
Length:555
Mass (Da):61,904
Last modified:July 5, 2004 - v1
Checksum:i456E284566D5EFC8
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti259 – 2591I → T in BAB71044 (PubMed:14702039).Curated
Sequence conflicti334 – 3341L → P in AAQ92306 (Ref. 1) Curated
Sequence conflicti334 – 3341L → P in AAQ92307 (Ref. 1) Curated
Sequence conflicti334 – 3341L → P in AAQ92308 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY328891 mRNA. Translation: AAQ92306.1.
AY328892 mRNA. Translation: AAQ92307.1.
AY328893 mRNA. Translation: AAQ92308.1.
AK055911 mRNA. Translation: BAB71044.1.
AL357075 Genomic DNA. Translation: CAI14545.1.
CH471051 Genomic DNA. Translation: EAW47699.1.
BC060761 mRNA. Translation: AAH60761.1.
CCDSiCCDS5246.1.
RefSeqiNP_775786.2. NM_173515.2.
UniGeneiHs.16064.

Genome annotation databases

EnsembliENST00000607772; ENSP00000475915; ENSG00000153721.
GeneIDi154043.
KEGGihsa:154043.
UCSCiuc003qpy.4. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY328891 mRNA. Translation: AAQ92306.1.
AY328892 mRNA. Translation: AAQ92307.1.
AY328893 mRNA. Translation: AAQ92308.1.
AK055911 mRNA. Translation: BAB71044.1.
AL357075 Genomic DNA. Translation: CAI14545.1.
CH471051 Genomic DNA. Translation: EAW47699.1.
BC060761 mRNA. Translation: AAH60761.1.
CCDSiCCDS5246.1.
RefSeqiNP_775786.2. NM_173515.2.
UniGeneiHs.16064.

3D structure databases

ProteinModelPortaliQ6P9H4.
SMRiQ6P9H4. Positions 2-76.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi127531. 9 interactions.
IntActiQ6P9H4. 3 interactions.
STRINGi9606.ENSP00000356182.

PTM databases

iPTMnetiQ6P9H4.
PhosphoSiteiQ6P9H4.

Polymorphism and mutation databases

BioMutaiCNKSR3.
DMDMi74758311.

Proteomic databases

MaxQBiQ6P9H4.
PaxDbiQ6P9H4.
PeptideAtlasiQ6P9H4.
PRIDEiQ6P9H4.

Protocols and materials databases

DNASUi154043.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000607772; ENSP00000475915; ENSG00000153721.
GeneIDi154043.
KEGGihsa:154043.
UCSCiuc003qpy.4. human.

Organism-specific databases

CTDi154043.
GeneCardsiCNKSR3.
H-InvDBHIX0006311.
HGNCiHGNC:23034. CNKSR3.
HPAiCAB037133.
HPA046557.
HPA049651.
neXtProtiNX_Q6P9H4.
PharmGKBiPA134880153.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1738. Eukaryota.
ENOG41104C7. LUCA.
GeneTreeiENSGT00390000017199.
HOGENOMiHOG000015752.
HOVERGENiHBG051040.
InParanoidiQ6P9H4.
OrthoDBiEOG71ZP0W.
PhylomeDBiQ6P9H4.
TreeFamiTF326495.

Miscellaneous databases

ChiTaRSiCNKSR3. human.
GenomeRNAii154043.
PROiQ6P9H4.

Gene expression databases

BgeeiQ6P9H4.
CleanExiHS_CNKSR3.
HS_MAGI1.
ExpressionAtlasiQ6P9H4. baseline and differential.
GenevisibleiQ6P9H4. HS.

Family and domain databases

Gene3Di1.10.150.50. 1 hit.
2.30.42.10. 1 hit.
InterProiIPR010599. CNKSR2.
IPR017874. CRIC_domain.
IPR001478. PDZ.
IPR001660. SAM.
IPR013761. SAM/pointed.
[Graphical view]
PfamiPF10534. CRIC_ras_sig. 1 hit.
PF06663. DUF1170. 1 hit.
PF00595. PDZ. 1 hit.
PF00536. SAM_1. 1 hit.
[Graphical view]
SMARTiSM00228. PDZ. 1 hit.
SM00454. SAM. 1 hit.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 1 hit.
SSF50156. SSF50156. 1 hit.
PROSITEiPS51290. CRIC. 1 hit.
PS50106. PDZ. 1 hit.
PS50105. SAM_DOMAIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning, expression and comparison of human, rat and mouse ML (maguin-like) genes, and expression in mouse oogenesis."
    Tzolovsky G.I., McGurk L., Pathirana S., Slee R., Hillier S., Clinton M., Bownes M.
    Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  3. "The DNA sequence and analysis of human chromosome 6."
    Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D.
    , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.
    Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  6. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "Cnksr3 is a direct mineralocorticoid receptor target gene and plays a key role in the regulation of the epithelial sodium channel."
    Ziera T., Irlbacher H., Fromm A., Latouche C., Krug S.M., Fromm M., Jaisser F., Borden S.A.
    FASEB J. 23:3936-3946(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY ALDOSTERONE.

Entry informationi

Entry nameiCNKR3_HUMAN
AccessioniPrimary (citable) accession number: Q6P9H4
Secondary accession number(s): Q5SGD5, Q96N65
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: July 5, 2004
Last modified: June 8, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

The gene name MAGI1, shown in this entry as a synonym, is an obsolete human gene nomenclature committee-approved name. It should be noted that MAGI1 currently is the official name for the unrelated membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.