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Q6P9A2

- GLT18_HUMAN

UniProt

Q6P9A2 - GLT18_HUMAN

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Protein
Polypeptide N-acetylgalactosaminyltransferase 18
Gene
GALNT18, GALNTL4
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor.1 Publication

Catalytic activityi

UDP-N-acetyl-alpha-D-galactosamine + polypeptide = UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide.

Cofactori

Manganese By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei194 – 1941Substrate By similarity
Metal bindingi251 – 2511Manganese By similarity
Metal bindingi253 – 2531Manganese By similarity
Metal bindingi382 – 3821Manganese By similarity
Binding sitei385 – 3851Substrate By similarity
Binding sitei390 – 3901Substrate By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. polypeptide N-acetylgalactosaminyltransferase activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. protein glycosylation Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Lectin, Manganese, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_115606. O-linked glycosylation of mucins.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiCBM13. Carbohydrate-Binding Module Family 13.
GT27. Glycosyltransferase Family 27.

Names & Taxonomyi

Protein namesi
Recommended name:
Polypeptide N-acetylgalactosaminyltransferase 18 (EC:2.4.1.41)
Alternative name(s):
Polypeptide GalNAc transferase 18
Short name:
GalNAc-T18
Polypeptide GalNAc transferase-like protein 4
Short name:
GalNAc-T-like protein 4
Short name:
pp-GaNTase-like protein 4
Polypeptide N-acetylgalactosaminyltransferase-like protein 4
Protein-UDP acetylgalactosaminyltransferase-like protein 4
UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase-like protein 4
Gene namesi
Name:GALNT18
Synonyms:GALNTL4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 11

Organism-specific databases

HGNCiHGNC:30488. GALNT18.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1212Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei13 – 3523Helical; Signal-anchor for type II membrane protein; Reviewed prediction
Add
BLAST
Topological domaini36 – 607572Lumenal Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. Golgi membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134950929.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 607607Polypeptide N-acetylgalactosaminyltransferase 18
PRO_0000059141Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi144 ↔ 377 By similarity
Glycosylationi146 – 1461N-linked (GlcNAc...) Reviewed prediction
Glycosylationi195 – 1951N-linked (GlcNAc...) Reviewed prediction
Glycosylationi320 – 3201N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi368 ↔ 447 By similarity
Disulfide bondi482 ↔ 498 By similarity
Disulfide bondi530 ↔ 543 By similarity
Disulfide bondi571 ↔ 591 By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ6P9A2.
PRIDEiQ6P9A2.

PTM databases

PhosphoSiteiQ6P9A2.

Expressioni

Gene expression databases

ArrayExpressiQ6P9A2.
BgeeiQ6P9A2.
CleanExiHS_GALNTL4.
GenevestigatoriQ6P9A2.

Organism-specific databases

HPAiHPA012955.

Interactioni

Protein-protein interaction databases

STRINGi9606.ENSP00000227756.

Structurei

3D structure databases

ProteinModelPortaliQ6P9A2.
SMRiQ6P9A2. Positions 122-601.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini469 – 599131Ricin B-type lectin
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni153 – 267115Catalytic subdomain A
Add
BLAST
Regioni324 – 38562Catalytic subdomain B
Add
BLAST

Domaini

There are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding By similarity.
The ricin B-type lectin domain binds to GalNAc and contributes to the glycopeptide specificity By similarity.

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG123124.
HOGENOMiHOG000038228.
HOVERGENiHBG051699.
InParanoidiQ6P9A2.
KOiK00710.
OMAiAPDKKLE.
OrthoDBiEOG70087G.
PhylomeDBiQ6P9A2.
TreeFamiTF313267.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR001173. Glyco_trans_2-like.
IPR029044. Nucleotide-diphossugar_trans.
IPR000772. Ricin_B_lectin.
[Graphical view]
PfamiPF00535. Glycos_transf_2. 1 hit.
PF00652. Ricin_B_lectin. 1 hit.
[Graphical view]
SMARTiSM00458. RICIN. 1 hit.
[Graphical view]
SUPFAMiSSF50370. SSF50370. 1 hit.
SSF53448. SSF53448. 1 hit.
PROSITEiPS50231. RICIN_B_LECTIN. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q6P9A2-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MVCTRKTKTL VSTCVILSGM TNIICLLYVG WVTNYIASVY VRGQEPAPDK    50
KLEEDKGDTL KIIERLDHLE NVIKQHIQEA PAKPEEAEAE PFTDSSLFAH 100
WGQELSPEGR RVALKQFQYY GYNAYLSDRL PLDRPLPDLR PSGCRNLSFP 150
DSLPEVSIVF IFVNEALSVL LRSIHSAMER TPPHLLKEII LVDDNSSNEE 200
LKEKLTEYVD KVNSQKPGFI KVVRHSKQEG LIRSRVSGWR AATAPVVALF 250
DAHVEFNVGW AEPVLTRIKE NRKRIISPSF DNIKYDNFEI EEYPLAAQGF 300
DWELWCRYLN PPKAWWKLEN STAPIRSPAL IGCFIVDRQY FQEIGLLDEG 350
MEVYGGENVE LGIRVWQCGG SVEVLPCSRI AHIERAHKPY TEDLTAHVRR 400
NALRVAEVWM DEFKSHVYMA WNIPQEDSGI DIGDITARKA LRKQLQCKTF 450
RWYLVSVYPE MRMYSDIIAY GVLQNSLKTD LCLDQGPDTE NVPIMYICHG 500
MTPQNVYYTS SQQIHVGILS PTVDDDDNRC LVDVNSRPRL IECSYAKAKR 550
MKLHWQFSQG GPIQNRKSKR CLELQENSDL EFGFQLVLQK CSGQHWSITN 600
VLRSLAS 607
Length:607
Mass (Da):69,561
Last modified:October 17, 2006 - v2
Checksum:i8FFA7BCB5016FF0C
GO
Isoform 2 (identifier: Q6P9A2-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     79-91: EAPAKPEEAEAEP → GYRRNFSLLNVSN
     92-607: Missing.

Note: No experimental confirmation available.

Show »
Length:91
Mass (Da):10,397
Checksum:i4EBD1A8E212F25C7
GO

Sequence cautioni

The sequence AAD20062.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei79 – 9113EAPAK…AEAEP → GYRRNFSLLNVSN in isoform 2.
VSP_011234Add
BLAST
Alternative sequencei92 – 607516Missing in isoform 2.
VSP_011235Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti133 – 1331D → G in AAH60864. 1 Publication
Sequence conflicti541 – 5411I → T in AAH60864. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF131852 mRNA. Translation: AAD20062.1. Different initiation.
BC060864 mRNA. Translation: AAH60864.1.
BC037341 mRNA. Translation: AAH37341.3.
CCDSiCCDS7807.1. [Q6P9A2-1]
RefSeqiNP_940918.2. NM_198516.2. [Q6P9A2-1]
UniGeneiHs.655152.

Genome annotation databases

EnsembliENST00000227756; ENSP00000227756; ENSG00000110328. [Q6P9A2-1]
GeneIDi374378.
KEGGihsa:374378.
UCSCiuc001mjo.2. human. [Q6P9A2-1]

Polymorphism databases

DMDMi116242498.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

GGDB

GlycoGene database

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF131852 mRNA. Translation: AAD20062.1 . Different initiation.
BC060864 mRNA. Translation: AAH60864.1 .
BC037341 mRNA. Translation: AAH37341.3 .
CCDSi CCDS7807.1. [Q6P9A2-1 ]
RefSeqi NP_940918.2. NM_198516.2. [Q6P9A2-1 ]
UniGenei Hs.655152.

3D structure databases

ProteinModelPortali Q6P9A2.
SMRi Q6P9A2. Positions 122-601.
ModBasei Search...

Protein-protein interaction databases

STRINGi 9606.ENSP00000227756.

Protein family/group databases

CAZyi CBM13. Carbohydrate-Binding Module Family 13.
GT27. Glycosyltransferase Family 27.

PTM databases

PhosphoSitei Q6P9A2.

Polymorphism databases

DMDMi 116242498.

Proteomic databases

PaxDbi Q6P9A2.
PRIDEi Q6P9A2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000227756 ; ENSP00000227756 ; ENSG00000110328 . [Q6P9A2-1 ]
GeneIDi 374378.
KEGGi hsa:374378.
UCSCi uc001mjo.2. human. [Q6P9A2-1 ]

Organism-specific databases

CTDi 374378.
GeneCardsi GC11M011293.
HGNCi HGNC:30488. GALNT18.
HPAi HPA012955.
neXtProti NX_Q6P9A2.
PharmGKBi PA134950929.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG123124.
HOGENOMi HOG000038228.
HOVERGENi HBG051699.
InParanoidi Q6P9A2.
KOi K00710.
OMAi APDKKLE.
OrthoDBi EOG70087G.
PhylomeDBi Q6P9A2.
TreeFami TF313267.

Enzyme and pathway databases

UniPathwayi UPA00378 .
Reactomei REACT_115606. O-linked glycosylation of mucins.

Miscellaneous databases

ChiTaRSi GALNTL4. human.
GenomeRNAii 374378.
NextBioi 100162.
PROi Q6P9A2.

Gene expression databases

ArrayExpressi Q6P9A2.
Bgeei Q6P9A2.
CleanExi HS_GALNTL4.
Genevestigatori Q6P9A2.

Family and domain databases

Gene3Di 3.90.550.10. 1 hit.
InterProi IPR001173. Glyco_trans_2-like.
IPR029044. Nucleotide-diphossugar_trans.
IPR000772. Ricin_B_lectin.
[Graphical view ]
Pfami PF00535. Glycos_transf_2. 1 hit.
PF00652. Ricin_B_lectin. 1 hit.
[Graphical view ]
SMARTi SM00458. RICIN. 1 hit.
[Graphical view ]
SUPFAMi SSF50370. SSF50370. 1 hit.
SSF53448. SSF53448. 1 hit.
PROSITEi PS50231. RICIN_B_LECTIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Mei G., Yu W., Gibbs R.A.
    Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Brain.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Placenta.
  3. "UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferases: completion of the family tree."
    Raman J., Guan Y., Perrine C.L., Gerken T.A., Tabak L.A.
    Glycobiology 22:768-777(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiGLT18_HUMAN
AccessioniPrimary (citable) accession number: Q6P9A2
Secondary accession number(s): O95903, Q8NDY9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: October 17, 2006
Last modified: September 3, 2014
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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