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Q6P9A2

- GLT18_HUMAN

UniProt

Q6P9A2 - GLT18_HUMAN

Protein

Polypeptide N-acetylgalactosaminyltransferase 18

Gene

GALNT18

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 103 (01 Oct 2014)
      Sequence version 2 (17 Oct 2006)
      Previous versions | rss
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    Functioni

    Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor.1 Publication

    Catalytic activityi

    UDP-N-acetyl-alpha-D-galactosamine + polypeptide = UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide.

    Cofactori

    Manganese.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei194 – 1941SubstrateBy similarity
    Metal bindingi251 – 2511ManganeseBy similarity
    Metal bindingi253 – 2531ManganeseBy similarity
    Metal bindingi382 – 3821ManganeseBy similarity
    Binding sitei385 – 3851SubstrateBy similarity
    Binding sitei390 – 3901SubstrateBy similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. polypeptide N-acetylgalactosaminyltransferase activity Source: UniProtKB-EC

    GO - Biological processi

    1. protein glycosylation Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Ligandi

    Lectin, Manganese, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_115606. O-linked glycosylation of mucins.
    UniPathwayiUPA00378.

    Protein family/group databases

    CAZyiCBM13. Carbohydrate-Binding Module Family 13.
    GT27. Glycosyltransferase Family 27.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Polypeptide N-acetylgalactosaminyltransferase 18 (EC:2.4.1.41)
    Alternative name(s):
    Polypeptide GalNAc transferase 18
    Short name:
    GalNAc-T18
    Polypeptide GalNAc transferase-like protein 4
    Short name:
    GalNAc-T-like protein 4
    Short name:
    pp-GaNTase-like protein 4
    Polypeptide N-acetylgalactosaminyltransferase-like protein 4
    Protein-UDP acetylgalactosaminyltransferase-like protein 4
    UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase-like protein 4
    Gene namesi
    Name:GALNT18
    Synonyms:GALNTL4
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 11

    Organism-specific databases

    HGNCiHGNC:30488. GALNT18.

    Subcellular locationi

    GO - Cellular componenti

    1. Golgi membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA134950929.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 607607Polypeptide N-acetylgalactosaminyltransferase 18PRO_0000059141Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi144 ↔ 377PROSITE-ProRule annotation
    Glycosylationi146 – 1461N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi195 – 1951N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi320 – 3201N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi368 ↔ 447PROSITE-ProRule annotation
    Disulfide bondi482 ↔ 498PROSITE-ProRule annotation
    Disulfide bondi530 ↔ 543PROSITE-ProRule annotation
    Disulfide bondi571 ↔ 591PROSITE-ProRule annotation

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiQ6P9A2.
    PRIDEiQ6P9A2.

    PTM databases

    PhosphoSiteiQ6P9A2.

    Expressioni

    Gene expression databases

    ArrayExpressiQ6P9A2.
    BgeeiQ6P9A2.
    CleanExiHS_GALNTL4.
    GenevestigatoriQ6P9A2.

    Organism-specific databases

    HPAiHPA012955.

    Interactioni

    Protein-protein interaction databases

    STRINGi9606.ENSP00000227756.

    Structurei

    3D structure databases

    ProteinModelPortaliQ6P9A2.
    SMRiQ6P9A2. Positions 122-601.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 1212CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini36 – 607572LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei13 – 3523Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini469 – 599131Ricin B-type lectinPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni153 – 267115Catalytic subdomain AAdd
    BLAST
    Regioni324 – 38562Catalytic subdomain BAdd
    BLAST

    Domaini

    There are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding.By similarity
    The ricin B-type lectin domain binds to GalNAc and contributes to the glycopeptide specificity.By similarity

    Sequence similaritiesi

    Contains 1 ricin B-type lectin domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG123124.
    HOGENOMiHOG000038228.
    HOVERGENiHBG051699.
    InParanoidiQ6P9A2.
    KOiK00710.
    OMAiAPDKKLE.
    OrthoDBiEOG70087G.
    PhylomeDBiQ6P9A2.
    TreeFamiTF313267.

    Family and domain databases

    Gene3Di3.90.550.10. 1 hit.
    InterProiIPR001173. Glyco_trans_2-like.
    IPR029044. Nucleotide-diphossugar_trans.
    IPR000772. Ricin_B_lectin.
    [Graphical view]
    PfamiPF00535. Glycos_transf_2. 1 hit.
    PF00652. Ricin_B_lectin. 1 hit.
    [Graphical view]
    SMARTiSM00458. RICIN. 1 hit.
    [Graphical view]
    SUPFAMiSSF50370. SSF50370. 1 hit.
    SSF53448. SSF53448. 1 hit.
    PROSITEiPS50231. RICIN_B_LECTIN. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q6P9A2-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MVCTRKTKTL VSTCVILSGM TNIICLLYVG WVTNYIASVY VRGQEPAPDK    50
    KLEEDKGDTL KIIERLDHLE NVIKQHIQEA PAKPEEAEAE PFTDSSLFAH 100
    WGQELSPEGR RVALKQFQYY GYNAYLSDRL PLDRPLPDLR PSGCRNLSFP 150
    DSLPEVSIVF IFVNEALSVL LRSIHSAMER TPPHLLKEII LVDDNSSNEE 200
    LKEKLTEYVD KVNSQKPGFI KVVRHSKQEG LIRSRVSGWR AATAPVVALF 250
    DAHVEFNVGW AEPVLTRIKE NRKRIISPSF DNIKYDNFEI EEYPLAAQGF 300
    DWELWCRYLN PPKAWWKLEN STAPIRSPAL IGCFIVDRQY FQEIGLLDEG 350
    MEVYGGENVE LGIRVWQCGG SVEVLPCSRI AHIERAHKPY TEDLTAHVRR 400
    NALRVAEVWM DEFKSHVYMA WNIPQEDSGI DIGDITARKA LRKQLQCKTF 450
    RWYLVSVYPE MRMYSDIIAY GVLQNSLKTD LCLDQGPDTE NVPIMYICHG 500
    MTPQNVYYTS SQQIHVGILS PTVDDDDNRC LVDVNSRPRL IECSYAKAKR 550
    MKLHWQFSQG GPIQNRKSKR CLELQENSDL EFGFQLVLQK CSGQHWSITN 600
    VLRSLAS 607
    Length:607
    Mass (Da):69,561
    Last modified:October 17, 2006 - v2
    Checksum:i8FFA7BCB5016FF0C
    GO
    Isoform 2 (identifier: Q6P9A2-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         79-91: EAPAKPEEAEAEP → GYRRNFSLLNVSN
         92-607: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:91
    Mass (Da):10,397
    Checksum:i4EBD1A8E212F25C7
    GO

    Sequence cautioni

    The sequence AAD20062.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti133 – 1331D → G in AAH60864. (PubMed:15489334)Curated
    Sequence conflicti541 – 5411I → T in AAH60864. (PubMed:15489334)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei79 – 9113EAPAK…AEAEP → GYRRNFSLLNVSN in isoform 2. 1 PublicationVSP_011234Add
    BLAST
    Alternative sequencei92 – 607516Missing in isoform 2. 1 PublicationVSP_011235Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF131852 mRNA. Translation: AAD20062.1. Different initiation.
    BC060864 mRNA. Translation: AAH60864.1.
    BC037341 mRNA. Translation: AAH37341.3.
    CCDSiCCDS7807.1. [Q6P9A2-1]
    RefSeqiNP_940918.2. NM_198516.2. [Q6P9A2-1]
    UniGeneiHs.655152.

    Genome annotation databases

    EnsembliENST00000227756; ENSP00000227756; ENSG00000110328. [Q6P9A2-1]
    GeneIDi374378.
    KEGGihsa:374378.
    UCSCiuc001mjo.2. human. [Q6P9A2-1]

    Polymorphism databases

    DMDMi116242498.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Web resourcesi

    GGDB

    GlycoGene database

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF131852 mRNA. Translation: AAD20062.1 . Different initiation.
    BC060864 mRNA. Translation: AAH60864.1 .
    BC037341 mRNA. Translation: AAH37341.3 .
    CCDSi CCDS7807.1. [Q6P9A2-1 ]
    RefSeqi NP_940918.2. NM_198516.2. [Q6P9A2-1 ]
    UniGenei Hs.655152.

    3D structure databases

    ProteinModelPortali Q6P9A2.
    SMRi Q6P9A2. Positions 122-601.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9606.ENSP00000227756.

    Protein family/group databases

    CAZyi CBM13. Carbohydrate-Binding Module Family 13.
    GT27. Glycosyltransferase Family 27.

    PTM databases

    PhosphoSitei Q6P9A2.

    Polymorphism databases

    DMDMi 116242498.

    Proteomic databases

    PaxDbi Q6P9A2.
    PRIDEi Q6P9A2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000227756 ; ENSP00000227756 ; ENSG00000110328 . [Q6P9A2-1 ]
    GeneIDi 374378.
    KEGGi hsa:374378.
    UCSCi uc001mjo.2. human. [Q6P9A2-1 ]

    Organism-specific databases

    CTDi 374378.
    GeneCardsi GC11M011293.
    HGNCi HGNC:30488. GALNT18.
    HPAi HPA012955.
    neXtProti NX_Q6P9A2.
    PharmGKBi PA134950929.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG123124.
    HOGENOMi HOG000038228.
    HOVERGENi HBG051699.
    InParanoidi Q6P9A2.
    KOi K00710.
    OMAi APDKKLE.
    OrthoDBi EOG70087G.
    PhylomeDBi Q6P9A2.
    TreeFami TF313267.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .
    Reactomei REACT_115606. O-linked glycosylation of mucins.

    Miscellaneous databases

    ChiTaRSi GALNTL4. human.
    GenomeRNAii 374378.
    NextBioi 100162.
    PROi Q6P9A2.

    Gene expression databases

    ArrayExpressi Q6P9A2.
    Bgeei Q6P9A2.
    CleanExi HS_GALNTL4.
    Genevestigatori Q6P9A2.

    Family and domain databases

    Gene3Di 3.90.550.10. 1 hit.
    InterProi IPR001173. Glyco_trans_2-like.
    IPR029044. Nucleotide-diphossugar_trans.
    IPR000772. Ricin_B_lectin.
    [Graphical view ]
    Pfami PF00535. Glycos_transf_2. 1 hit.
    PF00652. Ricin_B_lectin. 1 hit.
    [Graphical view ]
    SMARTi SM00458. RICIN. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50370. SSF50370. 1 hit.
    SSF53448. SSF53448. 1 hit.
    PROSITEi PS50231. RICIN_B_LECTIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Mei G., Yu W., Gibbs R.A.
      Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Brain.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Placenta.
    3. "UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferases: completion of the family tree."
      Raman J., Guan Y., Perrine C.L., Gerken T.A., Tabak L.A.
      Glycobiology 22:768-777(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiGLT18_HUMAN
    AccessioniPrimary (citable) accession number: Q6P9A2
    Secondary accession number(s): O95903, Q8NDY9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 16, 2004
    Last sequence update: October 17, 2006
    Last modified: October 1, 2014
    This is version 103 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 11
      Human chromosome 11: entries, gene names and cross-references to MIM
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3