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Protein

Polypeptide N-acetylgalactosaminyltransferase 18

Gene

GALNT18

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor.1 Publication

Catalytic activityi

UDP-N-acetyl-alpha-D-galactosamine + polypeptide = UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide.

Cofactori

Mn2+By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei194 – 1941SubstrateBy similarity
Metal bindingi251 – 2511ManganeseBy similarity
Metal bindingi253 – 2531ManganeseBy similarity
Metal bindingi382 – 3821ManganeseBy similarity
Binding sitei385 – 3851SubstrateBy similarity
Binding sitei390 – 3901SubstrateBy similarity

GO - Molecular functioni

  1. carbohydrate binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. polypeptide N-acetylgalactosaminyltransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. protein glycosylation Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Lectin, Manganese, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_115606. O-linked glycosylation of mucins.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiCBM13. Carbohydrate-Binding Module Family 13.
GT27. Glycosyltransferase Family 27.

Names & Taxonomyi

Protein namesi
Recommended name:
Polypeptide N-acetylgalactosaminyltransferase 18 (EC:2.4.1.41)
Alternative name(s):
Polypeptide GalNAc transferase 18
Short name:
GalNAc-T18
Polypeptide GalNAc transferase-like protein 4
Short name:
GalNAc-T-like protein 4
Short name:
pp-GaNTase-like protein 4
Polypeptide N-acetylgalactosaminyltransferase-like protein 4
Protein-UDP acetylgalactosaminyltransferase-like protein 4
UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase-like protein 4
Gene namesi
Name:GALNT18
Synonyms:GALNTL4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 11

Organism-specific databases

HGNCiHGNC:30488. GALNT18.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1212CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei13 – 3523Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini36 – 607572LumenalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. Golgi membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134950929.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 607607Polypeptide N-acetylgalactosaminyltransferase 18PRO_0000059141Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi144 ↔ 377PROSITE-ProRule annotation
Glycosylationi146 – 1461N-linked (GlcNAc...)Sequence Analysis
Glycosylationi195 – 1951N-linked (GlcNAc...)Sequence Analysis
Glycosylationi320 – 3201N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi368 ↔ 447PROSITE-ProRule annotation
Disulfide bondi482 ↔ 498PROSITE-ProRule annotation
Disulfide bondi530 ↔ 543PROSITE-ProRule annotation
Disulfide bondi571 ↔ 591PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ6P9A2.
PRIDEiQ6P9A2.

PTM databases

PhosphoSiteiQ6P9A2.

Expressioni

Gene expression databases

BgeeiQ6P9A2.
CleanExiHS_GALNTL4.
ExpressionAtlasiQ6P9A2. baseline and differential.
GenevestigatoriQ6P9A2.

Organism-specific databases

HPAiHPA012955.

Interactioni

Protein-protein interaction databases

BioGridi131894. 6 interactions.
STRINGi9606.ENSP00000227756.

Structurei

3D structure databases

ProteinModelPortaliQ6P9A2.
SMRiQ6P9A2. Positions 122-601.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini469 – 599131Ricin B-type lectinPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni153 – 267115Catalytic subdomain AAdd
BLAST
Regioni324 – 38562Catalytic subdomain BAdd
BLAST

Domaini

There are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding.By similarity
The ricin B-type lectin domain binds to GalNAc and contributes to the glycopeptide specificity.By similarity

Sequence similaritiesi

Contains 1 ricin B-type lectin domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG123124.
GeneTreeiENSGT00670000097647.
HOGENOMiHOG000038228.
HOVERGENiHBG051699.
InParanoidiQ6P9A2.
KOiK00710.
OMAiAPDKKLE.
OrthoDBiEOG70087G.
PhylomeDBiQ6P9A2.
TreeFamiTF313267.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR001173. Glyco_trans_2-like.
IPR029044. Nucleotide-diphossugar_trans.
IPR000772. Ricin_B_lectin.
[Graphical view]
PfamiPF00535. Glycos_transf_2. 1 hit.
PF00652. Ricin_B_lectin. 1 hit.
[Graphical view]
SMARTiSM00458. RICIN. 1 hit.
[Graphical view]
SUPFAMiSSF50370. SSF50370. 1 hit.
SSF53448. SSF53448. 1 hit.
PROSITEiPS50231. RICIN_B_LECTIN. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6P9A2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVCTRKTKTL VSTCVILSGM TNIICLLYVG WVTNYIASVY VRGQEPAPDK
60 70 80 90 100
KLEEDKGDTL KIIERLDHLE NVIKQHIQEA PAKPEEAEAE PFTDSSLFAH
110 120 130 140 150
WGQELSPEGR RVALKQFQYY GYNAYLSDRL PLDRPLPDLR PSGCRNLSFP
160 170 180 190 200
DSLPEVSIVF IFVNEALSVL LRSIHSAMER TPPHLLKEII LVDDNSSNEE
210 220 230 240 250
LKEKLTEYVD KVNSQKPGFI KVVRHSKQEG LIRSRVSGWR AATAPVVALF
260 270 280 290 300
DAHVEFNVGW AEPVLTRIKE NRKRIISPSF DNIKYDNFEI EEYPLAAQGF
310 320 330 340 350
DWELWCRYLN PPKAWWKLEN STAPIRSPAL IGCFIVDRQY FQEIGLLDEG
360 370 380 390 400
MEVYGGENVE LGIRVWQCGG SVEVLPCSRI AHIERAHKPY TEDLTAHVRR
410 420 430 440 450
NALRVAEVWM DEFKSHVYMA WNIPQEDSGI DIGDITARKA LRKQLQCKTF
460 470 480 490 500
RWYLVSVYPE MRMYSDIIAY GVLQNSLKTD LCLDQGPDTE NVPIMYICHG
510 520 530 540 550
MTPQNVYYTS SQQIHVGILS PTVDDDDNRC LVDVNSRPRL IECSYAKAKR
560 570 580 590 600
MKLHWQFSQG GPIQNRKSKR CLELQENSDL EFGFQLVLQK CSGQHWSITN

VLRSLAS
Length:607
Mass (Da):69,561
Last modified:October 17, 2006 - v2
Checksum:i8FFA7BCB5016FF0C
GO
Isoform 2 (identifier: Q6P9A2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     79-91: EAPAKPEEAEAEP → GYRRNFSLLNVSN
     92-607: Missing.

Note: No experimental confirmation available.

Show »
Length:91
Mass (Da):10,397
Checksum:i4EBD1A8E212F25C7
GO

Sequence cautioni

The sequence AAD20062.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti133 – 1331D → G in AAH60864 (PubMed:15489334).Curated
Sequence conflicti541 – 5411I → T in AAH60864 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei79 – 9113EAPAK…AEAEP → GYRRNFSLLNVSN in isoform 2. 1 PublicationVSP_011234Add
BLAST
Alternative sequencei92 – 607516Missing in isoform 2. 1 PublicationVSP_011235Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF131852 mRNA. Translation: AAD20062.1. Different initiation.
BC060864 mRNA. Translation: AAH60864.1.
BC037341 mRNA. Translation: AAH37341.3.
CCDSiCCDS7807.1. [Q6P9A2-1]
RefSeqiNP_940918.2. NM_198516.2. [Q6P9A2-1]
UniGeneiHs.655152.

Genome annotation databases

EnsembliENST00000227756; ENSP00000227756; ENSG00000110328. [Q6P9A2-1]
GeneIDi374378.
KEGGihsa:374378.
UCSCiuc001mjo.2. human. [Q6P9A2-1]

Polymorphism databases

DMDMi116242498.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

GGDB

GlycoGene database

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF131852 mRNA. Translation: AAD20062.1. Different initiation.
BC060864 mRNA. Translation: AAH60864.1.
BC037341 mRNA. Translation: AAH37341.3.
CCDSiCCDS7807.1. [Q6P9A2-1]
RefSeqiNP_940918.2. NM_198516.2. [Q6P9A2-1]
UniGeneiHs.655152.

3D structure databases

ProteinModelPortaliQ6P9A2.
SMRiQ6P9A2. Positions 122-601.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi131894. 6 interactions.
STRINGi9606.ENSP00000227756.

Protein family/group databases

CAZyiCBM13. Carbohydrate-Binding Module Family 13.
GT27. Glycosyltransferase Family 27.

PTM databases

PhosphoSiteiQ6P9A2.

Polymorphism databases

DMDMi116242498.

Proteomic databases

PaxDbiQ6P9A2.
PRIDEiQ6P9A2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000227756; ENSP00000227756; ENSG00000110328. [Q6P9A2-1]
GeneIDi374378.
KEGGihsa:374378.
UCSCiuc001mjo.2. human. [Q6P9A2-1]

Organism-specific databases

CTDi374378.
GeneCardsiGC11M011293.
HGNCiHGNC:30488. GALNT18.
HPAiHPA012955.
MIMi615136. gene.
neXtProtiNX_Q6P9A2.
PharmGKBiPA134950929.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG123124.
GeneTreeiENSGT00670000097647.
HOGENOMiHOG000038228.
HOVERGENiHBG051699.
InParanoidiQ6P9A2.
KOiK00710.
OMAiAPDKKLE.
OrthoDBiEOG70087G.
PhylomeDBiQ6P9A2.
TreeFamiTF313267.

Enzyme and pathway databases

UniPathwayiUPA00378.
ReactomeiREACT_115606. O-linked glycosylation of mucins.

Miscellaneous databases

GenomeRNAii374378.
NextBioi100162.
PROiQ6P9A2.
SOURCEiSearch...

Gene expression databases

BgeeiQ6P9A2.
CleanExiHS_GALNTL4.
ExpressionAtlasiQ6P9A2. baseline and differential.
GenevestigatoriQ6P9A2.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR001173. Glyco_trans_2-like.
IPR029044. Nucleotide-diphossugar_trans.
IPR000772. Ricin_B_lectin.
[Graphical view]
PfamiPF00535. Glycos_transf_2. 1 hit.
PF00652. Ricin_B_lectin. 1 hit.
[Graphical view]
SMARTiSM00458. RICIN. 1 hit.
[Graphical view]
SUPFAMiSSF50370. SSF50370. 1 hit.
SSF53448. SSF53448. 1 hit.
PROSITEiPS50231. RICIN_B_LECTIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Mei G., Yu W., Gibbs R.A.
    Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Brain.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Placenta.
  3. "UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferases: completion of the family tree."
    Raman J., Guan Y., Perrine C.L., Gerken T.A., Tabak L.A.
    Glycobiology 22:768-777(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiGLT18_HUMAN
AccessioniPrimary (citable) accession number: Q6P9A2
Secondary accession number(s): O95903, Q8NDY9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: October 17, 2006
Last modified: March 4, 2015
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.