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Q6P9A2 (GLT18_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Polypeptide N-acetylgalactosaminyltransferase 18

EC=2.4.1.41
Alternative name(s):
Polypeptide GalNAc transferase 18
Short name=GalNAc-T18
Polypeptide GalNAc transferase-like protein 4
Short name=GalNAc-T-like protein 4
Short name=pp-GaNTase-like protein 4
Polypeptide N-acetylgalactosaminyltransferase-like protein 4
Protein-UDP acetylgalactosaminyltransferase-like protein 4
UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase-like protein 4
Gene names
Name:GALNT18
Synonyms:GALNTL4
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length607 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Ref.3

Catalytic activity

UDP-N-acetyl-alpha-D-galactosamine + polypeptide = UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide.

Cofactor

Manganese By similarity.

Pathway

Protein modification; protein glycosylation.

Subcellular location

Golgi apparatus membrane; Single-pass type II membrane protein By similarity.

Domain

There are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding By similarity.

The ricin B-type lectin domain binds to GalNAc and contributes to the glycopeptide specificity By similarity.

Sequence similarities

Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily.

Contains 1 ricin B-type lectin domain.

Sequence caution

The sequence AAD20062.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q6P9A2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q6P9A2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     79-91: EAPAKPEEAEAEP → GYRRNFSLLNVSN
     92-607: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 607607Polypeptide N-acetylgalactosaminyltransferase 18
PRO_0000059141

Regions

Topological domain1 – 1212Cytoplasmic Potential
Transmembrane13 – 3523Helical; Signal-anchor for type II membrane protein; Potential
Topological domain36 – 607572Lumenal Potential
Domain469 – 599131Ricin B-type lectin
Region153 – 267115Catalytic subdomain A
Region324 – 38562Catalytic subdomain B

Sites

Metal binding2511Manganese By similarity
Metal binding2531Manganese By similarity
Metal binding3821Manganese By similarity
Binding site1941Substrate By similarity
Binding site3851Substrate By similarity
Binding site3901Substrate By similarity

Amino acid modifications

Glycosylation1461N-linked (GlcNAc...) Potential
Glycosylation1951N-linked (GlcNAc...) Potential
Glycosylation3201N-linked (GlcNAc...) Potential
Disulfide bond144 ↔ 377 By similarity
Disulfide bond368 ↔ 447 By similarity
Disulfide bond482 ↔ 498 By similarity
Disulfide bond530 ↔ 543 By similarity
Disulfide bond571 ↔ 591 By similarity

Natural variations

Alternative sequence79 – 9113EAPAK…AEAEP → GYRRNFSLLNVSN in isoform 2.
VSP_011234
Alternative sequence92 – 607516Missing in isoform 2.
VSP_011235

Experimental info

Sequence conflict1331D → G in AAH60864. Ref.2
Sequence conflict5411I → T in AAH60864. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 17, 2006. Version 2.
Checksum: 8FFA7BCB5016FF0C

FASTA60769,561
        10         20         30         40         50         60 
MVCTRKTKTL VSTCVILSGM TNIICLLYVG WVTNYIASVY VRGQEPAPDK KLEEDKGDTL 

        70         80         90        100        110        120 
KIIERLDHLE NVIKQHIQEA PAKPEEAEAE PFTDSSLFAH WGQELSPEGR RVALKQFQYY 

       130        140        150        160        170        180 
GYNAYLSDRL PLDRPLPDLR PSGCRNLSFP DSLPEVSIVF IFVNEALSVL LRSIHSAMER 

       190        200        210        220        230        240 
TPPHLLKEII LVDDNSSNEE LKEKLTEYVD KVNSQKPGFI KVVRHSKQEG LIRSRVSGWR 

       250        260        270        280        290        300 
AATAPVVALF DAHVEFNVGW AEPVLTRIKE NRKRIISPSF DNIKYDNFEI EEYPLAAQGF 

       310        320        330        340        350        360 
DWELWCRYLN PPKAWWKLEN STAPIRSPAL IGCFIVDRQY FQEIGLLDEG MEVYGGENVE 

       370        380        390        400        410        420 
LGIRVWQCGG SVEVLPCSRI AHIERAHKPY TEDLTAHVRR NALRVAEVWM DEFKSHVYMA 

       430        440        450        460        470        480 
WNIPQEDSGI DIGDITARKA LRKQLQCKTF RWYLVSVYPE MRMYSDIIAY GVLQNSLKTD 

       490        500        510        520        530        540 
LCLDQGPDTE NVPIMYICHG MTPQNVYYTS SQQIHVGILS PTVDDDDNRC LVDVNSRPRL 

       550        560        570        580        590        600 
IECSYAKAKR MKLHWQFSQG GPIQNRKSKR CLELQENSDL EFGFQLVLQK CSGQHWSITN 


VLRSLAS 

« Hide

Isoform 2 [UniParc].

Checksum: 4EBD1A8E212F25C7
Show »

FASTA9110,397

References

« Hide 'large scale' references
[1]Mei G., Yu W., Gibbs R.A.
Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Brain.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Placenta.
[3]"UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferases: completion of the family tree."
Raman J., Guan Y., Perrine C.L., Gerken T.A., Tabak L.A.
Glycobiology 22:768-777(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Web resources

GGDB

GlycoGene database

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF131852 mRNA. Translation: AAD20062.1. Different initiation.
BC060864 mRNA. Translation: AAH60864.1.
BC037341 mRNA. Translation: AAH37341.3.
RefSeqNP_940918.2. NM_198516.2.
UniGeneHs.655152.

3D structure databases

ProteinModelPortalQ6P9A2.
SMRQ6P9A2. Positions 103-601.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9606.ENSP00000227756.

Protein family/group databases

CAZyCBM13. Carbohydrate-Binding Module Family 13.
GT27. Glycosyltransferase Family 27.

PTM databases

PhosphoSiteQ6P9A2.

Polymorphism databases

DMDM116242498.

Proteomic databases

PaxDbQ6P9A2.
PRIDEQ6P9A2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000227756; ENSP00000227756; ENSG00000110328. [Q6P9A2-1]
GeneID374378.
KEGGhsa:374378.
UCSCuc001mjo.2. human. [Q6P9A2-1]

Organism-specific databases

CTD374378.
GeneCardsGC11M011293.
HGNCHGNC:30488. GALNT18.
HPAHPA012955.
neXtProtNX_Q6P9A2.
PharmGKBPA134950929.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG123124.
HOGENOMHOG000038228.
HOVERGENHBG051699.
InParanoidQ6P9A2.
KOK00710.
OMAAPDKKLE.
OrthoDBEOG70087G.
PhylomeDBQ6P9A2.
TreeFamTF313267.

Enzyme and pathway databases

ReactomeREACT_17015. Metabolism of proteins.
UniPathwayUPA00378.

Gene expression databases

ArrayExpressQ6P9A2.
BgeeQ6P9A2.
CleanExHS_GALNTL4.
GenevestigatorQ6P9A2.

Family and domain databases

InterProIPR001173. Glyco_trans_2-like.
IPR000772. Ricin_B_lectin.
[Graphical view]
PfamPF00535. Glycos_transf_2. 1 hit.
PF00652. Ricin_B_lectin. 1 hit.
[Graphical view]
SMARTSM00458. RICIN. 1 hit.
[Graphical view]
SUPFAMSSF50370. SSF50370. 1 hit.
PROSITEPS50231. RICIN_B_LECTIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSGALNTL4. human.
GenomeRNAi374378.
NextBio100162.
PROQ6P9A2.

Entry information

Entry nameGLT18_HUMAN
AccessionPrimary (citable) accession number: Q6P9A2
Secondary accession number(s): O95903, Q8NDY9
Entry history
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: October 17, 2006
Last modified: April 16, 2014
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Human chromosome 11

Human chromosome 11: entries, gene names and cross-references to MIM