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Protein

Hydroxyacylglutathione hydrolase, mitochondrial

Gene

hagh

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid.

Catalytic activityi

S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate.

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi97 – 971Zinc 1; via tele nitrogenBy similarity
Metal bindingi99 – 991Zinc 1; via pros nitrogenBy similarity
Metal bindingi101 – 1011Zinc 2By similarity
Metal bindingi102 – 1021Zinc 2; via tele nitrogenBy similarity
Metal bindingi153 – 1531Zinc 1; via tele nitrogenBy similarity
Metal bindingi177 – 1771Zinc 1By similarity
Metal bindingi177 – 1771Zinc 2By similarity
Metal bindingi216 – 2161Zinc 2; via tele nitrogenBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-DRE-70268. Pyruvate metabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxyacylglutathione hydrolase, mitochondrial (EC:3.1.2.6)
Alternative name(s):
Glyoxalase II
Short name:
Glx II
Gene namesi
Name:hagh
ORF Names:zgc:73161
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Chromosome 3

Organism-specific databases

ZFINiZDB-GENE-030131-8921. hagh.

Subcellular locationi

Isoform 1 :
  • Mitochondrion matrix By similarity
Isoform 2 :
  • Cytoplasm By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 303Hydroxyacylglutathione hydrolase, mitochondrialPRO_0000382743
Transit peptidei1 – ?MitochondrionSequence analysis

Proteomic databases

PaxDbiQ6P963.

Expressioni

Gene expression databases

BgeeiQ6P963.

Interactioni

Protein-protein interaction databases

STRINGi7955.ENSDARP00000034038.

Structurei

3D structure databases

ProteinModelPortaliQ6P963.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni186 – 1883Substrate bindingBy similarity
Regioni216 – 2183Substrate bindingBy similarity
Regioni292 – 2954Substrate bindingBy similarity

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0813. Eukaryota.
COG0491. LUCA.
GeneTreeiENSGT00530000063033.
HOGENOMiHOG000058041.
InParanoidiQ6P963.
KOiK01069.
OMAiHVKCLST.
OrthoDBiEOG789CBX.
PhylomeDBiQ6P963.
TreeFamiTF105273.

Family and domain databases

Gene3Di3.60.15.10. 1 hit.
HAMAPiMF_01374. Glyoxalase_2.
InterProiIPR032282. HAGH_C.
IPR017782. Hydroxyacylglutathione_Hdrlase.
IPR001279. Metallo-B-lactamas.
[Graphical view]
PfamiPF16123. HAGH_C. 1 hit.
PF00753. Lactamase_B. 1 hit.
[Graphical view]
PIRSFiPIRSF005457. Glx. 1 hit.
SMARTiSM00849. Lactamase_B. 1 hit.
[Graphical view]
SUPFAMiSSF56281. SSF56281. 1 hit.
TIGRFAMsiTIGR03413. GSH_gloB. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform 1 (identifier: Q6P963-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MWFRSLAVSA CTVGVLGALS QKFAPTALFH SAIRKSSLVE QSDMKVELLP
60 70 80 90 100
ALTDNYMYLL IDEETKEAAI VDPVEPQKVV DAVKKHGVKL KTVLTTHHHW
110 120 130 140 150
DHAGGNEKLV KLMPGLTVYG GDDRVGALTQ KVTHYNTFKV GSLNVKCLFT
160 170 180 190 200
PCHTSGHICY FVTKENSTEA PAVFTGDTLF VAGCGKFFEG TADEMYKALI
210 220 230 240 250
EVLGRLPPET RVYCGHEYTI NNLKFARHVE PNNEVIRTKL AWAKEKYDNG
260 270 280 290 300
EPTIPSTVAE EFTFNPFMRV REKSVLEHAG TSDPIEAMRS IRKEKDGFRV

PKN
Length:303
Mass (Da):33,806
Last modified:September 1, 2009 - v2
Checksum:i0578D00267A11F85
GO
Isoform 2 (identifier: Q6P963-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-43: Missing.

Note: Produced by alternative initiation at Met-44 of isoform 1. Alternative initiation has been proven in human.
Show »
Length:260
Mass (Da):29,212
Checksum:i60367C46BF8D8F3B
GO

Sequence cautioni

The sequence AAH60913.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence AAH66607.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence AAQ97789.1 differs from that shown. Reason: Erroneous initiation. Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 4343Missing in isoform 2. 2 PublicationsVSP_037934Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY398356 mRNA. Translation: AAQ97789.1. Different initiation.
BC060913 mRNA. Translation: AAH60913.1. Different initiation.
BC066607 mRNA. Translation: AAH66607.1. Different initiation.
RefSeqiNP_956337.1. NM_200043.2. [Q6P963-2]
XP_005163922.1. XM_005163865.2. [Q6P963-1]
XP_009297606.1. XM_009299331.1. [Q6P963-1]
UniGeneiDr.114249.

Genome annotation databases

EnsembliENSDART00000034373; ENSDARP00000034038; ENSDARG00000025338. [Q6P963-1]
ENSDART00000134208; ENSDARP00000122766; ENSDARG00000025338. [Q6P963-1]
GeneIDi336977.
KEGGidre:336977.

Keywords - Coding sequence diversityi

Alternative initiation

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY398356 mRNA. Translation: AAQ97789.1. Different initiation.
BC060913 mRNA. Translation: AAH60913.1. Different initiation.
BC066607 mRNA. Translation: AAH66607.1. Different initiation.
RefSeqiNP_956337.1. NM_200043.2. [Q6P963-2]
XP_005163922.1. XM_005163865.2. [Q6P963-1]
XP_009297606.1. XM_009299331.1. [Q6P963-1]
UniGeneiDr.114249.

3D structure databases

ProteinModelPortaliQ6P963.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7955.ENSDARP00000034038.

Proteomic databases

PaxDbiQ6P963.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSDART00000034373; ENSDARP00000034038; ENSDARG00000025338. [Q6P963-1]
ENSDART00000134208; ENSDARP00000122766; ENSDARG00000025338. [Q6P963-1]
GeneIDi336977.
KEGGidre:336977.

Organism-specific databases

CTDi3029.
ZFINiZDB-GENE-030131-8921. hagh.

Phylogenomic databases

eggNOGiKOG0813. Eukaryota.
COG0491. LUCA.
GeneTreeiENSGT00530000063033.
HOGENOMiHOG000058041.
InParanoidiQ6P963.
KOiK01069.
OMAiHVKCLST.
OrthoDBiEOG789CBX.
PhylomeDBiQ6P963.
TreeFamiTF105273.

Enzyme and pathway databases

ReactomeiR-DRE-70268. Pyruvate metabolism.

Miscellaneous databases

NextBioi20812014.
PROiQ6P963.

Gene expression databases

BgeeiQ6P963.

Family and domain databases

Gene3Di3.60.15.10. 1 hit.
HAMAPiMF_01374. Glyoxalase_2.
InterProiIPR032282. HAGH_C.
IPR017782. Hydroxyacylglutathione_Hdrlase.
IPR001279. Metallo-B-lactamas.
[Graphical view]
PfamiPF16123. HAGH_C. 1 hit.
PF00753. Lactamase_B. 1 hit.
[Graphical view]
PIRSFiPIRSF005457. Glx. 1 hit.
SMARTiSM00849. Lactamase_B. 1 hit.
[Graphical view]
SUPFAMiSSF56281. SSF56281. 1 hit.
TIGRFAMsiTIGR03413. GSH_gloB. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Kidney marrow.
  2. NIH - Zebrafish Gene Collection (ZGC) project
    Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Eye and Kidney.

Entry informationi

Entry nameiGLO2_DANRE
AccessioniPrimary (citable) accession number: Q6P963
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 1, 2009
Last sequence update: September 1, 2009
Last modified: January 20, 2016
This is version 80 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Only one single gene encoding glyoxalase II has been identified in vertebrates. In yeast and higher plants, separate genes encode the cytosolic and mitochondrial forms of glyoxalase II.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.