Q6P963 (GLO2_DANRE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hydroxyacylglutathione hydrolase, mitochondrial
EC=3.1.2.6

Alternative name(s):
Glyoxalase II
Short name=Glx II

Gene names

Name:	hagh
ORF Names:	zgc:73161

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	303 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid.
Catalytic activity	S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate.
Cofactor	Binds 2 zinc ions per subunit By similarity.
Subcellular location	Isoform 1: Mitochondrion matrix By similarity. Isoform 2: Cytoplasm By similarity.
Sequence similarities	Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family.
Caution	Only one single gene encoding glyoxalase II has been identified in vertebrates. In yeast and higher plants, separate genes encode the cytosolic and mitochondrial forms of glyoxalase II.
Sequence caution	The sequence AAH60913.1 differs from that shown. Reason: Erroneous initiation. The sequence AAH66607.1 differs from that shown. Reason: Erroneous initiation. The sequence AAQ97789.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cytoplasm Mitochondrion
Coding sequence diversity	Alternative initiation
Domain	Transit peptide
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	hydroxyacylglutathione hydrolase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative initiation. [Align] [Select]

Isoform 1 (identifier: Q6P963-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q6P963-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-43: Missing.

Note: Produced by alternative initiation at Met-44 of isoform 1. Alternative initiation has been proven in human.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 303		Hydroxyacylglutathione hydrolase, mitochondrial		PRO_0000382743
Regions
	Region	186 – 188	3	Substrate By similarity
	Region	216 – 218	3	Substrate By similarity
	Region	292 – 295	4	Substrate By similarity
Sites
	Metal binding	97	1	Zinc 1 By similarity
	Metal binding	99	1	Zinc 1 By similarity
	Metal binding	101	1	Zinc 2 By similarity
	Metal binding	102	1	Zinc 2 By similarity
	Metal binding	153	1	Zinc 1 By similarity
	Metal binding	177	1	Zinc 1 By similarity
	Metal binding	177	1	Zinc 2 By similarity
	Metal binding	216	1	Zinc 2 By similarity
Natural variations
	Alternative sequence	1 – 43	43	Missing in isoform 2.		VSP_037934

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified September 1, 2009. Version 2. Checksum: 0578D00267A11F85	FASTA	303	33,806
	10 20 30 40 50 60 MWFRSLAVSA CTVGVLGALS QKFAPTALFH SAIRKSSLVE QSDMKVELLP ALTDNYMYLL 70 80 90 100 110 120 IDEETKEAAI VDPVEPQKVV DAVKKHGVKL KTVLTTHHHW DHAGGNEKLV KLMPGLTVYG 130 140 150 160 170 180 GDDRVGALTQ KVTHYNTFKV GSLNVKCLFT PCHTSGHICY FVTKENSTEA PAVFTGDTLF 190 200 210 220 230 240 VAGCGKFFEG TADEMYKALI EVLGRLPPET RVYCGHEYTI NNLKFARHVE PNNEVIRTKL 250 260 270 280 290 300 AWAKEKYDNG EPTIPSTVAE EFTFNPFMRV REKSVLEHAG TSDPIEAMRS IRKEKDGFRV PKN
	Isoform 2 [UniParc]. Checksum: 60367C46BF8D8F3B	FASTA	260	29,212
	10 20 30 40 50 60 MKVELLPALT DNYMYLLIDE ETKEAAIVDP VEPQKVVDAV KKHGVKLKTV LTTHHHWDHA 70 80 90 100 110 120 GGNEKLVKLM PGLTVYGGDD RVGALTQKVT HYNTFKVGSL NVKCLFTPCH TSGHICYFVT 130 140 150 160 170 180 KENSTEAPAV FTGDTLFVAG CGKFFEGTAD EMYKALIEVL GRLPPETRVY CGHEYTINNL 190 200 210 220 230 240 KFARHVEPNN EVIRTKLAWA KEKYDNGEPT IPSTVAEEFT FNPFMRVREK SVLEHAGTSD 250 260 PIEAMRSIRK EKDGFRVPKN

References

[1]

"Hematopoietic gene expression profile in zebrafish kidney marrow." Song H.-D., Sun X.-J., Deng M., Zhang G.-W., Zhou Y., Wu X.-Y., Sheng Y., Chen Y., Ruan Z., Jiang C.-L., Fan H.-Y., Zon L.I., Kanki J.P., Liu T.X., Look A.T., Chen Z. Proc. Natl. Acad. Sci. U.S.A. 101:16240-16245(2004) [PubMed: 15520368] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Kidney marrow.

[2]

NIH - Zebrafish Gene Collection (ZGC) project Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Eye and Kidney.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY398356 mRNA. Translation: AAQ97789.1. Different initiation. BC060913 mRNA. Translation: AAH60913.1. Different initiation. BC066607 mRNA. Translation: AAH66607.1. Different initiation.
IPI	IPI00483561. IPI00944319.
RefSeq	NP_956337.1. NM_200043.2.
UniGene	Dr.114249.
3D structure databases
HSSP	HSSP built from PDB template 1QH5 based on UniProtKB Q16775.
ModBase	Search...
Protein-protein interaction databases
STRING	Q6P963.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSDART00000034373; ENSDARP00000034038; ENSDARG00000025338. ENSDART00000123456; ENSDARP00000106852; ENSDARG00000025338. ENSDART00000134208; ENSDARP00000122766; ENSDARG00000025338.
GeneID	336977.
KEGG	dre:336977.
NMPDR	fig|7955.3.peg.17186.
Organism-specific databases
CTD	3029.
ZFIN	ZDB-GENE-030131-8921. hagh.
Phylogenomic databases
eggNOG	fiNOG06109.
GeneTree	ENSGT00530000063033.
HOGENOM	HBG753931.
InParanoid	Q6P963.
OMA	GSLNVKC.
Gene expression databases
Bgee	Q6P963.
Family and domain databases
InterPro	IPR001279. Beta-lactamas-like. IPR017782. Hydroxyacylglutathione_Hdrlase. [Graphical view]
KO	K01069.
Pfam	PF00753. Lactamase_B. 1 hit. [Graphical view]
SMART	SM00849. Lactamase_B. 1 hit. [Graphical view]
TIGRFAMs	TIGR03413. GSH_gloB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLO2_DANRE

Accession

Primary (citable) accession number: Q6P963

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 1, 2009
Last sequence update:	September 1, 2009
Last modified:	December 14, 2011
This is version 51 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program