Thioredoxin domain-containing protein 2

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Q6P902 (TXND2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 81. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Thioredoxin domain-containing protein 2

Alternative name(s):
Spermatid-specific thioredoxin-1
Short name=Sptrx-1
Thioredoxin-4

Gene names

Name:	Txndc2
Synonyms:	Sptrx, Sptrx1, Trx4

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	515 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probably plays a regulatory role in sperm development. May participate in regulation of fibrous sheath (FS) assembly by supporting the formation of disulfide bonds during sperm tail morphogenesis. May also be required to rectify incorrect disulfide pairing and generate suitable pairs between the FS constituents. Can reduce disulfide bonds in vitro in the presence of NADP and thioredoxin reductase.
Subcellular location	Cytoplasm.
Tissue specificity	Testis-specific. Strongly expressed in the testicular seminiferous tubules, mostly in the round spermatids. Ref.1 Ref.3
Developmental stage	Expressed during spermiogenesis, restricted to the postmeiotic phase of spermatogenesis. First detected in elongating spermatids tails during steps 9 and 10 and is prominent in this region during steps 11-16. Also weakly present in the cytoplasmic lobe of these spermatids. During the last steps of spermiogenesis (steps 17-19), it strongly diminishes in the tail but appears to increase or become concentrated in the shrinking cytoplasmic lobe. By the last step of spermiogenesis (late step 19), cytoplasmic localization is barely detectable in the resulting residual body but still detectable in the cytoplasmic droplet (at protein level). Detected in testis of pre-pubertal animals at very low level. Ref.3 Ref.4
Sequence similarities	Contains 1 thioredoxin domain.
Sequence caution	The sequence AAM94687.2 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Differentiation Spermatogenesis
Cellular component	Cytoplasm
Domain	Redox-active center Repeat
Molecular function	Developmental protein
PTM	Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW cell redox homeostasis Inferred from electronic annotation. Source: InterPro glycerol ether metabolic process Inferred from electronic annotation. Source: InterPro multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW spermatogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell motile cilium Inferred from direct assay Ref.1. Source: MGI nucleolus Inferred from electronic annotation. Source: Compara outer dense fiber Inferred from electronic annotation. Source: Compara
Molecular_function	electron carrier activity Inferred from electronic annotation. Source: InterPro protein disulfide isomerase activity Inferred from direct assay Ref.1. Source: MGI protein disulfide oxidoreductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 515

515

Thioredoxin domain-containing protein 2

PRO_0000120154

Regions

Repeat

Repeat

Repeat

Repeat

Repeat

Repeat

Repeat

Repeat

Repeat

Repeat

Repeat

Repeat

Repeat

Repeat

Repeat

Repeat

Repeat

Repeat

Repeat

Repeat

Repeat

Domain

118

Thioredoxin

Region

92 – 405

314

21 X 15 AA approximate tandem repeat of Q-P-K-X-G-D-I-P-K-S-[PS]-E-[KE]-X-I

Amino acid modifications

Disulfide bond

442 ↔ 445

Redox-active By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q6P902 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 46F178558C27A71A
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FASTA

515

57,767

        10         20         30         40         50         60 
MTLNNGGKAN ERGSNENPLQ ALSKNEAFLV PEFLDTAQSK EKAIASKVSN TLHMSTEESE 

        70         80         90        100        110        120 
FPQQVSSTPM FSENTVHPRH EVSPKPSSKN TQLKQENISK SSGYSKQTNY SNTPKSLAKT 

       130        140        150        160        170        180 
THPKQGSTLK PATNSTHYRE DDIPKSSEDI IQPKKGDRPK SSEDIIQSKK EDRPKSSEDI 

       190        200        210        220        230        240 
IQSKKEDRPK SSEDIIQSKK EDRPKSSEDI IQSKKEDRPK SSEDIIQPKK EDRPKSSEDS 

       250        260        270        280        290        300 
VPSKKGDRPK SSEDSVQPKK EDRPKSSEDS VQSKEGEVHK PLKDSIQSKE TKVPKSPQDS 

       310        320        330        340        350        360 
IQSKEDKTHR PLKDSVQSKE SEEPKSSHES IQSKEDKIHK PLKDSIPSKE GDIPKSPEDT 

       370        380        390        400        410        420 
IQSQEEITAS EEDTIQSQEG NTIKSSEEDV QLSESKLLGL GAEIETLEEG LVRVIKDKEE 

       430        440        450        460        470        480 
FEEVLKDAGE KLVAVDFSAA WCGPCRMMKP LFHSLSLKHE DVIFLEVDTE DCEQLVQDCE 

       490        500        510 
IFHLPTFQFY KNEEKVGEFS GALVGKLERS ISELK

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, expression and characterization of mouse spermatid specific thioredoxin-1 gene and protein." Jimenez A., Oko R., Gustafsson J.-A., Spyrou G., Pelto-Huikko M., Miranda-Vizuete A. Mol. Hum. Reprod. 8:710-718(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, ENZYME ACTIVITY IN VITRO.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
[3]	"Developmental expression of spermatid-specific thioredoxin-1 protein: transient association to the longitudinal columns of the fibrous sheath during sperm tail formation." Yu Y., Oko R., Miranda-Vizuete A. Biol. Reprod. 67:1546-1554(2002) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[4]	"Absolute mRNA levels and transcriptional regulation of the mouse testis-specific thioredoxins." Jimenez A., Prieto-Alamo M.J., Fuentes-Almagro C.A., Jurado J., Gustafsson J.-A., Pueyo C., Miranda-Vizuete A. Biochem. Biophys. Res. Commun. 330:65-74(2005) [PubMed] [Europe PMC] [Abstract] Cited for: DEVELOPMENTAL STAGE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF196282 mRNA. Translation: AAM94687.2. Different initiation. BC060981 mRNA. Translation: AAH60981.1.
IPI	IPI00453741.
RefSeq	NP_001139474.1. NM_001146002.1. NP_705739.2. NM_153519.2.
UniGene	Mm.255732.
3D structure databases
ProteinModelPortal	Q6P902.
SMR	Q6P902. Positions 411-514.
ModBase	Search...
PTM databases
PhosphoSite	Q6P902.
Proteomic databases
PaxDb	Q6P902.
PRIDE	Q6P902.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	213272.
KEGG	mmu:213272.
UCSC	uc008dge.2. mouse.
Organism-specific databases
CTD	84203.
MGI	MGI:2389312. Txndc2.
Phylogenomic databases
eggNOG	COG0526.
HOGENOM	HOG000154719.
HOVERGEN	HBG087115.
InParanoid	Q6P902.
OrthoDB	EOG47PX7J.
Gene expression databases
ArrayExpress	Q6P902.
Bgee	Q6P902.
CleanEx	MM_TXNDC2.
Genevestigator	Q6P902.
GermOnline	ENSMUSG00000050612. Mus musculus.
Family and domain databases
Gene3D	3.40.30.10. 1 hit.
InterPro	IPR005746. Thioredoxin. IPR012336. Thioredoxin-like_fold. IPR013766. Thioredoxin_domain. [Graphical view]
PANTHER	PTHR10438. PTHR10438. 1 hit.
Pfam	PF00085. Thioredoxin. 1 hit. [Graphical view]
SUPFAM	SSF52833. Thiordxn-like_fd. 1 hit.
PROSITE	PS00194. THIOREDOXIN_1. False negative. PS51352. THIOREDOXIN_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	373905.
SOURCE	Search...

Entry information

Entry name

TXND2_MOUSE

Accession

Primary (citable) accession number: Q6P902
Secondary accession number(s): Q8CJD1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 5, 2005
Last sequence update:	July 5, 2004
Last modified:	April 3, 2013
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents